Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	844,900	T→C	D194G (GAC→GGC)	ybiO ←	moderate conductance mechanosensitive channel YbiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	844,900	0	T	C	100.0%	155.9 / NA	56	D194G (GAC→GGC)	ybiO	moderate conductance mechanosensitive channel YbiO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/26);  total (30/26)

ATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGA  >  NC_000913/844657‑845140                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 aTCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGataat                                                                                                                                                                                                                                           >  1:15007/1‑251 (MQ=255)        tGCTTAACCAGCTCAGGCGACGACTCCAGTAACGTGCGCTCTCGTCCTGCATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATCAACGcc                                                                                                                                                                                                                                    >  2:156237/1‑251 (MQ=255)                     caggcaccgacTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTGGAGAATCAAACTTTGTTGGAAAGCGATTGTGCGACTGCCTGCATTCAGGTTGTCTCTTGATGCCTGGCCGACAAAGAATGGCAGTGCGTGTAACAGTATGCCGATAATAACCGCCCCGATAatcat                                                                                                                                                                                                                         <  1:188218/242‑1 (MQ=255)                     cAGGCGACGCCTCCAGTAACGGGCACCCTCGTCCGGAACCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAatcatc                                                                                                                                                                                                                        <  2:143133/250‑1 (MQ=255)                             gacTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCCAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGc                                                                                                                                                                                                               >  1:42951/1‑251 (MQ=255)                                      aaCGGGCGCACTCGTCCTGCATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCCATGAGGGCAACGGCGTTGCGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCATGTTGTCGCTTACTACCTGGCCGACACATAATGTCAGGGCCAGTAACAGCAGGCCGATACTACACGCCCCGATACTCATCGCCGGACGCTGCAAcc                                                                                                                                                                                                        >  2:168324/1‑249 (MQ=255)                                                   gTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCAc                                                                                                                                                                                         >  1:40277/1‑251 (MQ=255)                                                        gAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGAtt                                                                                                                                                                                     <  1:405006/250‑1 (MQ=255)                                                                  cGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCcgcg                                                                                                                                                                          <  2:130267/251‑1 (MQ=255)                                                                   gggCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCgcgc                                                                                                                                                                         >  1:363148/1‑251 (MQ=255)                                                                        cAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACt                                                                                                                                                                    <  1:136797/251‑1 (MQ=255)                                                                               gCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCCATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGGAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCAt                                                                                                                                                             >  1:119856/1‑251 (MQ=255)                                                                               gCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATTCCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAAGCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCAt                                                                                                                                                             <  1:297645/251‑1 (MQ=255)                                                                                    gTTTGGGCAAAAAATCAGGGGGAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGTGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGc                                                                                                                                                        <  2:208391/251‑1 (MQ=255)                                                                                             aaaaaTCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCg                                                                                                                                               >  2:205863/1‑251 (MQ=255)                                                                                              acaaTCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGATGCAACCAGTTGCTGCGCTCACGCTTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCgg                                                                                                                                              >  2:2445/3‑251 (MQ=255)                                                                                               aaaTCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATGCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCGGCAACCAGTTGCGGCGCTCACGATGTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGc                                                                                                                                             <  1:249650/251‑1 (MQ=255)                                                                                                 aTCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGACAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGc                                                                                                                                                        >  2:407212/1‑238 (MQ=255)                                                                                                 aTCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGc                                                                                                                                                        <  1:407212/238‑1 (MQ=255)                                                                                                    aGGCGTAGTACGGCTTTGCAAAATTCAATGAGGGCAAAGGCGTTGAGAACCAAACTTTGTTGGAAAGCGCTGGAGCGCCTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACCAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGCAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCCCCCACTGACCCATTTTGCGATACAGGGGCAGTgc                                                                                                                                        >  2:215955/1‑251 (MQ=255)                                                                                                        gtagtaCGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGcac                                                                                                                                    <  1:112232/251‑1 (MQ=255)                                                                                                             aagggtttGAAAAATGCAAGGAGGGCAAAGGGGTGTATAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCTCTTAATACCGGTCCGACAAAGCATGTCAGGGCCAGTAACAACAGGCCGATAATAAACGCCCCGATAATCAGCGCCGGAATCGGCGGCCAGTTGCTGCGCTCACGGGTTTTTGGCCGCGCCCACTGACCCATGTTGCGATACAGCTGCATTGCTCACAgacg                                                                                                                               <  2:284508/246‑4 (MQ=255)                                                                                                             aCGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCg                                                                                                                               >  1:134913/1‑251 (MQ=255)                                                                                                                gCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAAt                                                                                                                            <  1:7700/251‑1 (MQ=255)                                                                                                                 cTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATc                                                                                                                           >  1:158490/1‑251 (MQ=255)                                                                                                                         aaTTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTa                                                                                                                   <  1:265490/251‑1 (MQ=255)                                                                                                                            tCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCCCAGGCGAATCAGCCAGTaac                                                                                                                 >  2:185334/1‑249 (MQ=255)                                                                                                                              aaTGAGGGCAAAGGCGTTGAGAAGCAAACTTTGTTGGAAAGCGATTGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCATCCAGTTGCTGCGCTCACGAGTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAAc                                                                                                              <  1:292249/251‑1 (MQ=255)                                                                                                                               aTGAGTGCAGAGGCGTAAAGAAACCAACATTGTTAGCAAGCGATGGTGCGGCTGCCTGCATGCAGGCTGAAGGTAACTACCTAGCCTACAAAAAATCTCAGCCCCCGGCACAGCCGCCCGCTAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAATTATTACGATCACGATCTTTTTGCTGAGCCGACTTACCCATTTTGGCATGCAGCGGCAGCGCGGACAGGCGACTAAGCCAGTAAAAAcc                                                                                                             >  2:222099/1‑251 (MQ=255)                                                                                                                                 gAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCAGTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCaa                                                                                                           <  2:152362/251‑1 (MQ=255)                                                                                                                                    ggCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAac                                                                                                         >  1:222727/1‑250 (MQ=255)                                                                                                                                         aGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCGGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAAt                                                                                                   <  1:212297/251‑1 (MQ=255)                                                                                                                                            cgtagggaAACAACCTTGGTTGGAAAGCAATGGTGCGACTGCCTGCGTTCAGTGTGTCGCTTAATACCTGTCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGGGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACATGCGAATCAGCCAGTAAAAACCAAACACTAATAcc                                                                                                <  2:226217/245‑1 (MQ=255)                                                                                                                                                gagaAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCt                                                                                             >  1:470148/1‑250 (MQ=255)                                                                                                                                                      caaacTTTGTTGGAAAGCGAGGGGGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTGTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTg                                                                                      <  1:313656/251‑1 (MQ=255)                                                                                                                                                             tgttgGCACGCGATGGAGCGACTGCCTGCATTCAGGATGTCGCTTAATAACTGGTCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGAGCATAAACGACCCGATAATCATCGCCGGCAGCTGCAACCAGTTGCAGAGATCCCGATTTTTTTGCCGCGCCCACAGACCCTTTTTGCGATACAACCGGAGTGCGCACAGGCAAATCAGCCAGTAAAACCCAAACACTATTACCGATAACATTGacacatg                                                                               >  2:410559/1‑245 (MQ=255)                                                                                                                                                              gttgGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATgg                                                                              >  1:121338/1‑251 (MQ=255)                                                                                                                                                                 ggAAAGCGATGGGGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCGGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAGCCAGTTGCTGCGCTCACGATTGTTTTGCCGGGCCCACGGACCCATTTTGCGATACAGCGGCAGTGCGGACAGGCGAAGCAGCCAGTAAAAACCAAACACTAATACCGGTAACAGTGAAAAAGGGGTc                                                                           <  2:42951/251‑1 (MQ=255)                                                                                                                                                                  gAAAGCGATGGTGAGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGCCAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATAGCCGGAAGCTGCAACCAGTTGCTGCGCTCACCATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATggggc                                                                           >  2:338487/1‑248 (MQ=255)                                                                                                                                                                   aaaGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAAt                                                                                                   >  1:270388/1‑225 (MQ=255)                                                                                                                                                                   aaaGCGATGGGTCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAAt                                                                                                   <  2:270388/225‑1 (MQ=255)                                                                                                                                                                        gATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGCTAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATCCCGCTAACATTGAAAAATGGGTCAGCGCAt                                                                    >  1:405165/1‑251 (MQ=255)                                                                                                                                                                                gACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAAACAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGa                                                              >  1:471065/1‑249 (MQ=255)                                                                                                                                                                                    gcctgcATTCAGGTTGTCGATTAATACCTGGCCGACAAATACTGTAAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATACTCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCCGTGCGCACAGGCGAATCAGCCAGTAAAAACCACACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTgtcggttt                                                        >  2:481728/1‑244 (MQ=255)                                                                                                                                                                                      ctgcATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTtgt                                                      >  1:306862/1‑251 (MQ=255)                                                                                                                                                                                        gcATTCAGGTTGTCGCTGAATACCTGACCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCTCATTGCTGAAGGTTtgt                                                      <  2:280418/249‑1 (MQ=255)                                                                                                                                                                                          aTTCAGGTTGTCGCTTAATACCAGGCCGACAAATACTGTCAGGGCCAGTAACAGCAGGCAGATACTAAACGCCCCGATAATCAACGCGGGAAGCTACAACCAGTTGATGCGCTCACGATTTTTTTGACGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAAACCTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGAGGAt                                                  >  2:61906/1‑251 (MQ=255)                                                                                                                                                                                             cAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTa                                                >  1:201537/1‑250 (MQ=255)                                                                                                                                                                                                     cGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCTCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCAGTGCTGAAGGTTTGTGGATTAAACGGCtt                                        <  1:145493/250‑1 (MQ=255)                                                                                                                                                                                                      gCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTAt                                      >  2:178510/1‑251 (MQ=255)                                                                                                                                                                                                             aCCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCgggg                                <  2:96695/250‑1 (MQ=255)                                                                                                                                                                                                                    cGACAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTg                        <  2:167028/251‑1 (MQ=255)                                                                                                                                                                                                                      aCAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAAACAGTTGCTGCCCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGat                      <  1:254230/251‑1 (MQ=255)                                                                                                                                                                                                                       cAAATAATGTCAGGGCCAGTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGat                      >  2:3032/1‑250 (MQ=255)                                                                                                                                                                                                                                         gTAACAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCGTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCg   <  1:178510/251‑1 (MQ=255)                                                                                                                                                                                                                                            aCAGCAGGCCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGa  >  1:152822/1‑249 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 ATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGA  >  NC_000913/844657‑845140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑