Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	499,060	T→G	D305A (GAC→GCC)	aes ←	acetylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	499,060	0	T	G	100.0%	138.6 / NA	43	D305A (GAC→GCC)	aes	acetylesterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/22);  total (21/22)

GGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGACGGCTGTCATCCAGCAGCGGATCGAACTCCGCCCCGGCAATAAAACAGGGCGGAACTTCGCGAGTGAGATCATTATTAAACAGACAGTAATACGGCGACTCGCGGTCCGCGTCGTTGCTTAAATATGCCTCT  >  NC_000913/498817‑499299                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                ggAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGTCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGt                                                                                                                                                                                                                                          <  1:299514/251‑1 (MQ=255)    gaAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGtttt                                                                                                                                                                                                                                       <  1:269570/251‑1 (MQ=255)     aaaaaGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAAAATGAATATATTCCGGCGCTTAATGCCCCGCCGGAACCTATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAAATGAGCGCCGTCGCGGAGAGCCTCGGCGGCGGGTTtc                                                                                                                                                                                                                                      >  1:145636/1‑251 (MQ=255)      aaaaGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCGCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATAGCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCGGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCTGTTTtca                                                                                                                                                                                                                                     <  2:305885/251‑1 (MQ=255)                  tCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTTCCGCGCATCGCTAAAGCTGCGAGGGAAAGAAATGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGGTTTCATCATCCGTGt                                                                                                                                                                                                                           >  1:21361‑M1/1‑248 (MQ=255)                                                     aTTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGACGGAAAATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTTAGCGGTAAAGAAATGGGCGGCGTCGCGAAAAGCCTCGGCGGGGGTTTTCATCATCCGTGtagtggtaagcattagacattttgtcctgatgctacgc                                                                                                                                                                                      >  1:361547‑M1/1‑213 (MQ=255)                                                        gTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttg                                                                                                                                                                                   <  2:168411‑M1/251‑42 (MQ=255)                                                          cTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCCCGAAGAGCCTCGGCGGCGGTTTTCTTCATCCGTGttgtcgttggcattagacattttttccttattcgccgctttac                                                                                                                                                                                 >  1:115479‑M1/1‑208 (MQ=255)                                                            ggAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCGTCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgac                                                                                                                                                                                 <  1:288750‑M1/249‑44 (MQ=255)                                                                 cgcgggcagagattgccgAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAAGCTTGTTGCCGCGTATCGCTAAAGCTGATCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGAGtagtcggaggcattagacatttgtgccttatgcgccgcttgacgcgtctt                                                                                                                                                                          <  2:479786‑M1/244‑51 (MQ=255)                                                                 cGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtctt                                                                                                                                                                          <  1:352542‑M1/251‑51 (MQ=255)                                                                                    gCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtg                                                                                                                                                       <  2:145636‑M1/251‑70 (MQ=255)                                                                                            gTGAGGTCTTCCTCGGTGCCGGCGGGACAAAATATGAATATATTCCGGCGCTTAATGCCAAGCCCGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCCACCGCGtagtcgtaggcctttgacatttgtgcctgatgcgacgctttacgcgtcttatcaggcctacaaccggtgccccatcc                                                                                                                                               >  1:177595‑M1/1‑174 (MQ=255)                                                                                              gAGGTCTTCCGCGGGTCCGGCGGGAAAAAATATGAATATATGCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccgg                                                                                                                                             <  2:129289‑M1/251‑80 (MQ=255)                                                                                                      ccTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGTCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcagccggcaattgg                                                                                                                                      <  2:468459‑M1/250‑87 (MQ=255)                                                                                                                 gcggGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcct                                                                                                                          <  1:207005‑M1/251‑99 (MQ=255)                                                                                                                  cggGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcc                                                                                                                           >  1:106132‑M1/1‑152 (MQ=255)                                                                                                                  cggGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcc                                                                                                                           <  2:106132‑M1/249‑98 (MQ=255)                                                                                                                         aaaTATGAATATATTCCGGCGCTTAATGCCACGCCAGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGCCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtcggccttagacatttttgcctgatgcgccgcttgacgcgtcttatcaggcctacaaccggagccgcatcaggccattggtgccccatgcctgttgcg                                                                                                                    >  2:237655‑M1/1‑145 (MQ=255)                                                                                                                             atGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctt                                                                                                              >  1:398991‑M1/1‑141 (MQ=255)                                                                                                                                     atgccggcGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatc                                                                                                      <  2:400653‑M1/248‑119 (MQ=255)                                                                                                                                                 aaTGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcct                                                                                           <  1:258607‑M1/250‑130 (MQ=255)                                                                                                                                                 aaTGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcct                                                                                           <  1:222976‑M1/250‑130 (MQ=255)                                                                                                                                                  aTGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGAGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctac                                                                                         >  1:331574‑M1/1‑120 (MQ=255)                                                                                                                                                     ccACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggggcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaat                                                                                      >  1:378092‑M1/1‑117 (MQ=255)                                                                                                                                                          ccGGAACATATCGCAATGATGGCTACTCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggg                                                                                  >  1:399779‑M1/1‑112 (MQ=255)                                                                                                                                                          ccGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgtcgcttcttatcaggcctacaatgggt                                                                                  >  2:329308‑M1/1‑112 (MQ=255)                                                                                                                                                                   atCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcgg                                                                        >  1:479846‑M1/1‑103 (MQ=255)                                                                                                                                                                        aaTGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggc                                                                   >  1:412708‑M1/1‑98 (MQ=255)                                                                                                                                                                                cTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggata                                                             >  2:379364‑M1/1‑90 (MQ=255)                                                                                                                                                                                   aTCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcg                                                        >  1:98735‑M1/1‑87 (MQ=255)                                                                                                                                                                                        gttgCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaatttgtgcacaatgccttatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgttta                                                    <  1:30917‑M1/250‑169 (MQ=255)                                                                                                                                                                                           gCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgcc                                                >  2:96439‑M1/1‑79 (MQ=255)                                                                                                                                                                                           gCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgcc                                                >  1:175647‑M1/1‑79 (MQ=255)                                                                                                                                                                                           gCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACGGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCAGCCGTGtagtcgtaggcattagacatgtgtgcctgatgcggcgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgcc                                                <  2:326146‑M1/251‑173 (MQ=255)                                                                                                                                                                                                gTATCGCTACAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctaccatgggtaccggatcggtaggccggataaggcgtttacggcgcatc                                           >  2:106683‑M1/1‑74 (MQ=255)                                                                                                                                                                                                               aGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagc                            <  1:288968‑M1/251‑193 (MQ=255)                                                                                                                                                                                                                  ggTAAAGAACTGAGCGCCTTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcacc                         <  1:342746‑M1/251‑196 (MQ=255)                                                                                                                                                                                                                  ggTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcggttacgccgcatccggcaagaatcgcgcacc                         >  2:274696‑M1/1‑56 (MQ=255)                                                                                                                                                                                                                              aGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttggcgcatcttatcaggcctacaatggggaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaac             <  1:329308‑M1/251‑208 (MQ=255)                                                                                                                                                                                                                               gcgcCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccgacaagaatagagcaccagttaaccgaact            <  1:222220‑M1/251‑209 (MQ=255)                                                                                                                                                                                                                                    gTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaacttactc       <  1:126321‑M1/251‑214 (MQ=255)                                                                                                                                                                                                                                         gAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaacttactctgcgc  >  1:175246‑M1/1‑33 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                GGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGACGGCTGTCATCCAGCAGCGGATCGAACTCCGCCCCGGCAATAAAACAGGGCGGAACTTCGCGAGTGAGATCATTATTAAACAGACAGTAATACGGCGACTCGCGGTCCGCGTCGTTGCTTAAATATGCCTCT  >  NC_000913/498817‑499299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑