Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,278,657	T→C	V60A (GTT→GCT)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,278,657	0	T	C	98.5%	196.0 / NA	66	V60A (GTT→GCT)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (32/32);  minor base A (1/0);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAAC‑GTGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCT‑GGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGG  >  NC_000913/4278414‑4278902                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       tCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTTAATCGACGCCTGGTTTTAAATTTCACAAC‑CTGGAAGCACTGGCCCTCCGGGA‑GGGGTTG‑CC‑GGG‑GTAACAACGGTTCTGGGGGTGGTCTCCTCGGTTCTCGTCGTT‑CCAGGGCTGTTTGGTAAAAACGGCTTCCCCCCCGCGGCCGCTTTCGtt                                                                                                                                                                                                                                                 >  2:146229/1‑251 (MQ=255)    cTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCCACGCCTGGGTTTAAATTTCCCAAA‑GGGGGAGCACTGTCCGGCAGGAAAGGGGTT‑‑CC‑GGG‑GTAACAAACTTTCTTGCGATGGTCTACTCCGTCATCCTCGTTTCCAGG‑ATGTTGGGTAAAACAGGCGTCCCGCCTGCGGCCGCTTTCCTTGc                                                                                                                                                                                                                                               >  1:413358/1‑250 (MQ=255)              aTTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGCTGTTTTCTTATGTCTACGCCCTCAGCGCGTACCGGCGGTTAACTCGACGCCCGGGTTAAAAATTCACAAC‑GTGGGAGCCCTGTTCGCCCGGAA‑GTGGGTG‑CCCGGG‑TTA‑CAACGTTTCTGGGGCTGGGCTACTCTGCCATCGTCGTT‑CCAGGGATGTTGGGGAAAGAAGGCCTCCCGCCTCTGGCACCCTTCGTTTCAACCCGTCT‑gg                                                                                                                                                                                                                                   >  1:194370/1‑251 (MQ=255)                tGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAAC‑GTGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGt                                                                                                                                                                                                                                  <  1:255006/250‑1 (MQ=255)                                tGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGGCTACGCCATCAGCGCGTACCGGCGGTTCACGCGACGCCTGGTTTAAAATTTCACAAC‑GGGGAAGCACGGTCCGCCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCTATGGTCTTCTCGGTCATCGTCGTT‑CCAGGTATTTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGtt                                                                                                                                                                                                                    <  1:294107/248‑1 (MQ=255)                                 gACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATAAGCGCGTACCGGCGGATCCATCGCCGCCTGGCTTTAAATTTTACAAA‑GAGGAAGGACTCTCCCTCAAGAA‑GGGGCTG‑CC‑CGG‑GTAACAACGCTTCTCGCGGTGGGCTTCTCGGTCAACGTCGTT‑TCCGGGATATTGGGGAAAGCAGGACTCCCCCCCGACGCCGGCTTCGTTGCAACCTGTCTTGGTT‑CTGGGCTCGGTTCtct                                                                                                                                                                                                                >  1:421800/1‑249 (MQ=255)                                        aTTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTTACCCGACGCCTGGTTTAAAATTTCACAAA‑GTGGAAGCCCCGGCCGGCAGGAA‑GGGGTTG‑CC‑GGG‑GTAAAAACGTTTCTTGGGATGGTCTACTCGGTCATCGTCGGT‑CCCGGGATGTTGGGGAAAACAGGCTTCCCGCCTGCGGCCGCTTTCGTTTAAACCTGTCT‑GGGTGCTGGACCCGGttttctcggggtg                                                                                                                                                                                                         >  1:440127/1‑240 (MQ=255)                                                                ttATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAAC‑GTGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGtt                                                                                                                                                                                  <  1:96956/250‑1 (MQ=255)                                                                ttATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCCACGCCCGGGTTTAAATTTCACAAC‑GGGGGAGCACTGTCCGTCAGGAA‑GTGGGTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGGCATCGGCGTT‑CCAGGTATGTTTGGGAAAGGAGGGTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGGTTTATCGTGGTGGGTCTGTGGGCTAATCTGCCGTTg                                                                                                                                                                                 >  1:141866/1‑251 (MQ=255)                                                                    gTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACCCCCGGTTTTAAATTTTACAAC‑GTGGAAGCACTGTCCGTCAGGGA‑GTGGTTG‑CC‑GGGGTTAA‑AACGTTTTTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGGATGTTGGGGAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTTCTGGACTCGGGTCTTTCGTGGTGGGGCTGTGGGGTAATCTGCCGTTggggg                                                                                                                                                                             >  1:443318/1‑248 (MQ=255)                                                                                   gcgTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAAC‑GTGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCAtct                                                                                                                                                              <  1:150260/251‑1 (MQ=255)                                                                                     ggaCCGGCGGTTCACTCGACGCTTGGTTTAAAATTTAACAAC‑GGGGAGGCACGGTCCGTCAGGAA‑GTGGTTG‑AC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGTTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTTGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTcc                                                                                                                                                            <  2:189092/249‑1 (MQ=255)                                                                                            cggTTCACTCGACGCCTGGTTTAAAATTTCACAAC‑GTGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTTGCGATTGGTTGCGCCATCTCCCTGACAg                                                                                                                                                     <  1:396987/251‑1 (MQ=255)                                                                                              gTTCACTCGACGCCTGGTTTAAAATTTCACAAC‑GTGGCAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCTTCTCCCTGACAGCg                                                                                                                                                   >  2:152767/1‑251 (MQ=255)                                                                                                    ccGACGCGTGGTTTAAA‑TTTCCCAACCGTGGATGCTCTGTCCTTCAGGAA‑‑TGGTTGGCC‑GGT‑TTAACACCGTGTCTGGCGTTGGTCTACGCGGTCACCGTCGTT‑CGAGGGAGGTTGGTTAAAGCAGGCTCCCCGCCTGCGGCATCTTTCGTTGCAACCTGTCT‑TGTTGCTGGACTCGGTGCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTAcc                                                                                                                                             <  2:435274/250‑1 (MQ=255)                                                                                                          ccTGGTTTAAAATTTCACAAC‑GTGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACATGTCT‑GGTTGCTGGACTCGGTTCTATCGTGCTGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCAttt                                                                                                                                       >  2:173178/1‑250 (MQ=255)                                                                                                            tGGTTCAAACTTTCCCAAC‑GCGAAAGAACTGTCCGTCACGCC‑ATGGATG‑CC‑GGG‑TTATCAACGTTTCTCGCGATCGTCTACTCGATCATCGTCGTT‑CCCGGAATGTTGGGTACAGCAGGCTTCCCCACTGCGGCAGATTTAGTTGCCACCTGTCA‑GGTTGATGGACTCGGTTCTATCGTGCTGGGTCTGTGGGCTAATCTGCCGATGGCGGTTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCCTTc                                                                                                                                       >  2:214672/1‑249 (MQ=255)                                                                                                             ggTTTAAACTTTCACAAC‑GTGGAAGCACTGTCCGTAAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTCCTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATAGTGATGGGTCt                                                                                                                                                                                                    >  2:185881/1‑187 (MQ=255)                                                                                                             ggTTTAAAATTCCACAAC‑GTGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGTTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCt                                                                                                                                                                                                    <  1:185881/187‑1 (MQ=255)                                                                                                                ttAAAATTTCACAAC‑GGGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCt                                                                                                                                  <  1:263411/250‑1 (MQ=255)                                                                                                                    aaTTTCACAAC‑GTGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGc                                                                                                                             <  1:117952/251‑1 (MQ=255)                                                                                                                     aTTTCACAAC‑GGGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGTCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCt                                                                                                                            <  1:451201/251‑1 (MQ=255)                                                                                                                         cacaAC‑GGGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTTTACTCGGTCATCGTCGTT‑CCAGGTATTTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTgggg                                                                                                                        <  1:17151/251‑1 (MQ=255)                                                                                                                             aC‑GTGGAAGCACTGTCCGTAAGGAA‑GTGGATG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCCGGCTTCCCGCCTGCGGCAGCTTTCGTTGCCAACTGTCT‑GGTTGCTGGACTCGGTTCTCTCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGTTTGGTTGCGCCATCCCCCTGACAGCGTTTACCGCATTCCACCTGGTGCTGGGGCac                                                                                                                     >  2:480683/1‑249 (MQ=255)                                                                                                                                     aGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATAtt                                                                                                               >  2:388201/1‑249 (MQ=255)                                                                                                                                        aCTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAACCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGt                                                                                                          >  1:384030/1‑251 (MQ=255)                                                                                                                                          tGTCCGTCGGGAG‑GGGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTCCTCGGTCATGGTCTTT‑CCAGGTATGTTGGGAAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTa                                                                                                         <  2:443318/250‑1 (MQ=255)                                                                                                                                           gTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTCCTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGTTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTAcc                                                                                                       <  1:333082/251‑1 (MQ=255)                                                                                                                                               gTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTc                                                                                                   >  1:404477/1‑251 (MQ=255)                                                                                                                                                 cgggAA‑GTGGTTG‑CG‑GGG‑TTAACATCGTTTCTGGCATTGTTTTACTCGGTCATCGTCTTT‑CCAGGTATGTTGGTTAATGCAGGCTTCCCGCCGGCGTCAGCTTTCGTGGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAAGCTTCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCTCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTcgc                                                                                                 <  1:430974/249‑1 (MQ=255)                                                                                                                                                   ggAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCgcgc                                                                                               <  1:366116/251‑1 (MQ=255)                                                                                                                                                    gAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCt                                                                                              <  1:117690/251‑1 (MQ=255)                                                                                                                                                          ggTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTGGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTTTCGCGCTGGGTg                                                                                         >  2:146856/1‑251 (MQ=255)                                                                                                                                                           gTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTTCCTGTCGCGCTGGGTGc                                                                                        <  1:378157/251‑1 (MQ=255)                                                                                                                                                                 c‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCgtgt                                                                                   >  1:158240/1‑251 (MQ=255)                                                                                                                                                                   ggg‑TTAACAGCGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCAt                                                                                                                                         <  2:197582/196‑1 (MQ=255)                                                                                                                                                                   ggg‑TTAACACCTTTGCTGGGGATGGTCTCCTCGGGCATCGTGGTT‑CCAGGTATGGTGGGTAAAGCAGGCTGCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACGCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGGTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCGGGGTGCCgtg                                                                                    <  2:421800/249‑1 (MQ=255)                                                                                                                                                                   ggg‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCAt                                                                                                                                         >  1:197582/1‑196 (MQ=255)                                                                                                                                                                            aacGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGGGCCGTGTTCCTGATgg                                                                          >  2:378896/1‑251 (MQ=255)                                                                                                                                                                               gTTTCTGGCGATGGTCTACTCGGTCATCGGCGTT‑CCAGGTATGTGGGGTAAAGCAGGCTGCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACGCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGGTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGgtg                                                                       <  1:152767/251‑1 (MQ=255)                                                                                                                                                                                     ggCGATGGTCTACTCGGTCATCGTCGTG‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCGCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGc                                                                    <  1:306566/248‑1 (MQ=255)                                                                                                                                                                                      gcgagggccgaCTCGGTCATCGTCGTT‑CCAGTGATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACAGATTAGCGTACCTGTCGCGCTGGGTGCCGGGTTCCTGATGGGTGTGCTGtt                                                                <  2:194370/241‑1 (MQ=255)                                                                                                                                                                                        gATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGttt                                                               >  2:436463/1‑250 (MQ=255)                                                                                                                                                                                        gATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGttt                                                               >  1:196115/1‑250 (MQ=255)                                                                                                                                                                                         gtgtcatccTCGGTCGCCGTCGTT‑CCAGGTAGGTGGGGTAAAGCAGGCTGCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTTGCGTTCCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTTTGCTGTTTAc                                                             <  2:183702/243‑1 (MQ=255)                                                                                                                                                                                         aTGGTCCACACGGTCATCCTCGTA‑CACGGTATGCTGGGTGAACCAGGCTTCCCGCATCCGGCCGCTTTCGTTGCCCCACGTCT‑GGTTGCTGAACTCGGTTCTATCGGGACAGGTCTGTGGGCAAATCTGCCGTTGGCGATTGATTGCGCCATCTCCCTGACAGCGTTTACCGCTTTCAGCTTGGTGATGGGGCCACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGCTGGgtgcgtggtttac                                                             >  2:424053/1‑241 (MQ=255)                                                                                                                                                                                                 cTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAAttt                                                     >  1:255176/1‑251 (MQ=255)                                                                                                                                                                                                  tCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTc                                                    >  1:339112/1‑251 (MQ=255)                                                                                                                                                                                                   cGGTCATCGTCGTT‑CCAGTTATGTTGGGAAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCGCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCt                                                   <  2:76208/251‑1 (MQ=255)                                                                                                                                                                                                    ggTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTg                                                  <  1:341268/251‑1 (MQ=255)                                                                                                                                                                                                       cATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGcc                                                >  1:73315/1‑250 (MQ=255)                                                                                                                                                                                                             cgtT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGCTTGGTTGTGCCATCTCCCTGACAGCGTTTCCCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTTGGTGCCGTGTTCCTGCTGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGtt                                         >  2:15206/1‑250 (MQ=255)                                                                                                                                                                                                              gtT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTAt                                        >  1:116844/1‑251 (MQ=255)                                                                                                                                                                                                              gtT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTAt                                        >  2:106835/1‑251 (MQ=255)                                                                                                                                                                                                              cgtccaagtaaggttggtaaaaGCAGCCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGt                                          <  2:141866/231‑1 (MQ=255)                                                                                                                                                                                                                  ccAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGCTGGGGGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCg                                     >  1:274659/1‑251 (MQ=255)                                                                                                                                                                                                                    aggtattgtGGGTAAGGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCG‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTa                                   <  2:45974/243‑1 (MQ=255)                                                                                                                                                                                                                      gTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGc                                 >  1:53544/1‑251 (MQ=255)                                                                                                                                                                                                                            tGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCt                                                                   <  1:376490/211‑1 (MQ=255)                                                                                                                                                                                                                            tGGGTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCt                                                                   >  2:376490/1‑211 (MQ=255)                                                                                                                                                                                                                             gggTAAAGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGAtt                           <  1:436463/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaaGCAGGCTTCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTgcg                      <  2:12764/251‑1 (MQ=255)                                                                                                                                                                                                                                          ttCCCGCCTGCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCCGCCTGGTGCTGGGGCACCATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGATTACGGAAATTTCTGCCACGGGTATCAGTCGCTGGATTTTGCGCAACTTGcc             >  1:256201/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gCGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGgt      <  2:339112/249‑1 (MQ=255)                                                                                                                                                                                                                                                    cGGCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGAGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGtgtg   >  1:102508/1‑251 (MQ=255)                                                                                                                                                                                                                                                     ggCAGCTTTCGTTGCAACCTGTCT‑GGTTGCTGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTgg  >  2:340620/1‑251 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       TCGCTGGCAGGGGATTGTCCGCCACGCATCTTGACGAAAATTAAACTCTCAGGGGATGTTTTCTTATGTCTACGCCATCAGCGCGTACCGGCGGTTCACTCGACGCCTGGTTTAAAATTTCACAAC‑GTGGAAGCACTGTCCGTCAGGAA‑GTGGTTG‑CC‑GGG‑TTAACAACGTTTCTGGCGATGGTCTACTCGGTCATCGTCGTT‑CCAGGTATGTTGGGTAAAGCAGGCTTCCCGCCTGCGGCAGTTTTCGTTGCAACCTGTCT‑GGTTGCCGGACTCGGTTCTATCGTGATGGGTCTGTGGGCTAATCTGCCGTTGGCGATTGGTTGCGCCATCTCCCTGACAGCGTTTACCGCATTCAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGG  >  NC_000913/4278414‑4278902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑