| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,072,544 | T→C | A36A (GCA→GCG) | yihT ← | 6‑deoxy‑6‑sulfofructose‑1‑phosphate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,072,544 | 0 | T | C | 97.3% | 176.2 / NA | 73 | A36A (GCA→GCG) | yihT | 6‑deoxy‑6‑sulfofructose‑1‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (39/32); minor base G (2/0); total (41/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTG > NC_000913/4072297‑4072787 | cGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATCACCACGCTCTCGACCGGAATACCGTTGCCAGGAATGACCTCATCGGCGGCGAAAACCATGGCGAAACTTTTGGCAAACGCCTTTAGCTCCACC‑ACCTGGCGGTAGAAGAATTGTTGATCTACCAGCATCGCCGAGGCATAAGGCGAGAGGGACTTTGCAGCGTTAACTTTGAAATCCGTCAAAACGCTATCGGCTACCGGAGCGGGCGcc > 1:445457/1‑251 (MQ=255) gTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGccc > 1:435237/1‑251 (MQ=255) gTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGccc < 2:74152/251‑1 (MQ=255) gagcggTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCTCCCCAGCcgcg < 2:243805/245‑1 (MQ=255) aTCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGCGGCATAAGGCGCGAGGGGTTTTGCCGCGTTTAATTTGAAATCAGTTAAAACGCTATCGGCTAACGGAGCGGGCGCCCCAACCGCGGc > 2:86720/1‑251 (MQ=255) ccTGTAGCGGATTGATTTTGCGGTCAATAACCACGCGATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCa < 2:161915/250‑1 (MQ=255) gTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCgg < 1:102177/246‑1 (MQ=255) ggATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATAACGTTGCCAGGAATGAACTCATCGGCGGCGACAATAATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAAca‑tcct > 1:424024/1‑249 (MQ=255) attgattTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTGAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATgc < 2:446660/251‑1 (MQ=255) gCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCCTAAGGCGCGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCtt > 1:313920/1‑251 (MQ=255) ggTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCCGAATTGTTGCTCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCCGCCCCGGCAAACA‑TCATGCGCATGGCTTcg > 2:406747/1‑251 (MQ=255) aaCCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGGATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGGGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCCTGGGTTCGCGCTggt > 2:13680/1‑249 (MQ=255) ccACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACACTCCTGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCCTAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCCGTTAAAACGCTATCGGCCACCGGAGCGGGCGCCCCCGCCGCGGCAC‑CACTCATGCGCATGGCTTAGCGCTgctcc > 2:22062/1‑247 (MQ=255) ccACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCg < 1:368363/251‑1 (MQ=255) cACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCCGTTAAAACGCTATCGGCTACCGGCGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCg > 1:28594/1‑250 (MQ=255) aCGCTATCGACCGGAATACCGTTGCCAGGAATGCACTCATCGGCGGCGACAATCATGGCGCCACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTTAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGGGCCCCAGCCGCGGCAAACA‑TCCTGCGCCTGGCCTCGCGACGATCGa > 2:470077/1‑250 (MQ=255) cGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGTCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGAcc < 2:227272/251‑1 (MQ=255) acTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGTGACAATCATGGCGCCACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTTGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAAGCATAAGGCGAGAGGGCCTTTGCAGCGGTTACTTTGAAATCCGCTAAAACGCTATCGGCTAAAGGAGCGGGCGCCCCCGCCCCGGCAAACA‑TCATGCGCCTGGCTTCtcgcctctccaccg > 2:142514/2‑241 (MQ=255) cTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCt < 1:89144/234‑1 (MQ=255) cTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑CTATGCGCATGGCt > 2:89144/1‑234 (MQ=255) aTCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCa < 1:141673/251‑1 (MQ=255) cGACCGGAATCCCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCCACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGCATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCCGTTAAAACGCTATCGGCTACCGGAGCGGGGGCCCCAGCCGCGGCAAACC‑ACCTGCGCCTGGCTTcggcgcggtcgcccgccagg > 2:443846/1‑233 (MQ=255) gAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCCACTTTTGGCAATAGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCCGCCGCGGCAAACA‑TCCTGCGCCTGGCTTCCCGCTgcccgcccgcccgcactgc > 2:252947/1‑232 (MQ=255) aaTACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCa > 1:3507/1‑250 (MQ=255) aTACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATgggca > 1:61889/1‑247 (MQ=255) cGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCg < 2:99457/251‑1 (MQ=255) tGCCAGGAATGACCCCCTCGGCGGCGACAATCATGTCGAAACTTTTGCCAATCGCGTTTTGC‑CAACCCACCGGGCGGTAGCAGAATTGTTGATCTACCAGAATCCCCGAGGCATAAGGCGAGAGGGCCTTGGCAGCGTTAACTTTGAAATCAGTAAAAACGCTATCGGCTACCGGAGCGGCCGCCCCAGCCGCGGCAAACA‑TCATGCGCAGGGCTTCGCGCTAATCGACCGCCAGCATGGCAAAACCGcc < 2:435237/250‑1 (MQ=255) cggcggCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTc < 2:28594/250‑1 (MQ=255) cggcggCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCg < 1:406747/251‑1 (MQ=255) ggcggcGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTAGCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGt < 1:443846/251‑1 (MQ=255) aCAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGgt > 1:249409/1‑251 (MQ=255) aagcagggCGCAACTTTTGGCAAGCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGgtgt < 1:23238/245‑1 (MQ=255) aaTCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTCGCAGAATTGTTGGTCTACCAGAATCGCCGCGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCCTGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGgtgt > 1:173699/1‑251 (MQ=255) gcAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCCTGGCTTCGAGCTGATCGACCGCCAGGATGGCAAAACCGCCCGATGCGCGCGGCATGGCGTTGAGGGGGTACtttttgc > 2:204576/1‑246 (MQ=255) aCTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATATACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGtt > 1:420340/1‑231 (MQ=255) ttGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTcattcatt < 2:357949/250‑1 (MQ=255) tGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAg < 1:22062/250‑1 (MQ=255) cAACC‑CCCTGGCGGTAGCAGATTTGGTGATCTACCAAAATCGCCGAGGCAGAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAATTAAAACGCTATCGGCTACCGGGGCGGGCGCCCCAGCCGCGGCAAACA‑TCAGGCGCATGGCTTCGCGCTGATCTACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGtt < 2:459962/251‑1 (MQ=255) cAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAA‑CGCCATCCGCTAACCGAACGGGCGCCCCAACCGCGGCCAACC‑TCATGCCCATGGCCTCGCGCTGATCGACCGCCAGCATGGCAAAAACGCCCGAAGCGCGCGTAATGGCGTTGGTGGGGTACTTATTCATTCCTTTAATTCTTTATTCAGTCGttt > 1:340898/1‑250 (MQ=255) cAACC‑ACCTGGCGGTAGCAAGATTGTTAGTCGACCAGAATCGCCGAGGCAGAAGGCTATAGGCCCTTTGCAGCGTGATCTTTGAAATCAGTTAAACCGCTATCGGCTACCGGAGCGGGCGCCCCTGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCGGATCGCCCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATTGCGTTGATGCTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGtt < 1:65046/251‑1 (MQ=255) ggCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACAttttt > 1:171722/1‑250 (MQ=255) aGCAGATTTGTGGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGGGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATATTTACTTTg < 2:258856/250‑1 (MQ=255) aGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGCTCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTgg > 2:316967/1‑251 (MQ=255) aaTTGTTGATCTACCAGAATCGCCGGGGCATAAGGCGAGGGGGCCTTTGCAGCGTTAACTTTGAAATCAGGTAAAACGCTATCGGCTCCCGGAGCGGGCCCCCCAGCCCCGGCAAACA‑CCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGGAATGTCGTTGATGGTGTACTTATTCATTCATTTATGCCTTTATCCAGTCGTTACATTGTTACTTTGGCAGTc < 2:173699/251‑1 (MQ=255) tgtttATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAAGCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCAt < 2:365041/250‑1 (MQ=255) ttgATCTAACAGCATCGCCGAGGCCTAAGGCGAGAGGGCCTTTGAAGCGTTAACTTTGAAATCAGTTAACACGCTATCGGCTACCGGCGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCCTTTATTCCTTTATCCAGTCGTTACCTTTTTACTTTGGCAGTCATccc > 2:469214/1‑251 (MQ=255) acaagaataccagAGGCATAAGGCGCGAGGCCCTTTGCAGCGTTAACTTTGAAACCATTTAAAGCGCTTTCTGCTGCCGGGGCGGGCGCCCCAGCCGCTGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCTGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCgg < 2:1290/234‑1 (MQ=255) ccAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGa < 1:359529/251‑1 (MQ=255) ccAGAATCGCCGAGACCTAAGGCGAGCGGGCCTTTGCAGCGTTAACTTTGACATCAGTTCAAACGCTATCGGCTACCGGCGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGCTCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTACTGTCGTTGTTGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCCGTCATCCCGGCACTg > 2:300369/1‑250 (MQ=255) gAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAACTCCGTTAAAACGCTATCGGCTACCGGCGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGacac > 1:222193/1‑251 (MQ=255) gAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGacac > 1:46033/1‑251 (MQ=255) gggtaTAAGGCGAGAGGGCCTTTGCAGCGTTAACTGTGAAATCAGTAAAAACGCTATCGGCTACCGGAGGGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTGCATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGtt < 2:251005/247‑1 (MQ=255) aTAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTcc > 2:26346/1‑249 (MQ=255) aaGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCCACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGAGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAAACGCCCGCAGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGAAACGGACCTGGTCCAGa > 2:346370/1‑250 (MQ=255) gggCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGAcc > 1:8962/1‑251 (MQ=255) ccTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGt > 2:107066/1‑251 (MQ=255) ttGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTCCCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCTATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTcc < 1:311563/250‑1 (MQ=255) aGCGTTAACTTTGAAATCAGTTTAAACGCTATCGCCTACCGGATCGGGCGACCCACCAGCGGCAAACA‑TCATGCGCATGGCCTCGCGCTGCGCCACCGCCCGCATGGCAAAACCGCCCGATGCGCGCGTAATGTAGTTGATCGTGGACTTCTTCATTCATTTATTCCTTTATCCACTCGTTACATTTTTACTTTGACAGTCATCCCGGCACTGCCACGGACCTGTTCCAGAATGGCGGACCCGTCCTGgcg > 2:331162/1‑251 (MQ=255) aGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGgcg > 2:229883/1‑251 (MQ=255) aaCTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGTTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGTCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGgcgaccgc < 1:40294/250‑1 (MQ=255) tttGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGAGCCAAACA‑TCATGCGCATGGCCTCGCGCTGAACGACCGCCAGAATGGCACAACCGCCCGATGCGCGCGTAATGTCGTTGATGGGGTACTTCTTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGCCCTGTTCCAGAATGGCGGACCAGTCCTGgcgaccgcgccc > 1:219659/1‑248 (MQ=255) taaaaccAGTTAAAGCGCTACGGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGAGCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATGCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGAccc < 2:27633/244‑1 (MQ=255) cAGTTAAAACGCAATCGGCTGCCGGGGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCGTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATGCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCGGTTCCAGAATGGCTGACCAGTCCTGTCGACCGCGACCCGCTgcgc < 2:318957/251‑1 (MQ=255) aaaccgtATCGGCTACCGGCGCGGGCGCCCCAGCCCGGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCTACCCGCTGCGCGCGCCt < 2:291849/245‑1 (MQ=255) aCGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTgg > 2:175013/1‑251 (MQ=255) aCGCTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTgg > 1:272026/1‑251 (MQ=255) cTATCGGCTACCGGAGCGGGCGCCCCAGCCGCGGCACACC‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCt > 2:308663/1‑250 (MQ=255) ggCTACCGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAAc > 2:122796/1‑251 (MQ=255) aCCGGAGCGGGCGCACCAGCCGCGGCAAACC‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGCTGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGAACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGCCCCGCCGCGCGCGCCTGGCTATAAACCTcc > 2:207599/1‑251 (MQ=255) ccGGGGCGGGCGCCCCAGCCGCGGCAAACA‑CCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTccc < 2:171722/251‑1 (MQ=255) ccGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCTCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCAGCCCGGCACTGACACTGGCCTGTTCCAGAATGGCGGACCAGTCCTTGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTccc < 1:205895/251‑1 (MQ=255) cGGAGCGGGCGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCg > 1:143680/1‑251 (MQ=255) gAGCGGGCGCCCCAGCCGCGGCAAACA‑TCACGCGCATGGCTTCGTGTTGATCGACCGCCAGCACGGCAAAACCGTCCGATGCGCGCGTAATGTCGTTGATAGAGTACATATACACTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATAACGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGGCCGCGACCCGCAGCGCGAGACTGGCTATACACCTCCCGTg > 1:51231/1‑251 (MQ=255) | CGTCTTGTTTGATCTGTAGCGGATTGATTTTGCGGTCAATAACCACGCTATCGACCGGAATACCGTTGCCAGGAATGAACTCATCGGCGGCGACAATCATGGCGCAACTTTTGGCAATCGCGTTTTGCTCAACC‑ACCTGGCGGTAGCAGAATTGTTGATCTACCAGAATCGCCGAGGCATAAGGCGAGAGGGCCTTTGCAGCGTTAACTTTGAAATCAGTTAAAACGCTATCGGCTACCGGAGCGGGTGCCCCAGCCGCGGCAAACA‑TCATGCGCATGGCTTCGCGCTGATCGACCGCCAGCATGGCAAAACCGCCCGATGCGCGCGTAATGTCGTTGATGGTGTACTTATTCATTCATTTATTCCTTTATCCAGTCGTTACATTTTTACTTTGGCAGTCATCCCGGCACTGACACGGACCTGTTCCAGAATGGCGGACCAGTCCTGGCGACCGCGACCCGCTGCGCGCGCCTGGCTATAAACCTCCCGTG > NC_000913/4072297‑4072787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |