Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,393	G→A	intergenic (‑75/+20)	glyQ ← / ← ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,393	0	G	A	100.0%	174.2 / NA	47	intergenic (‑75/+20)	glyQ/ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

AGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTG  >  NC_000913/3725148‑3725631                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               agcagcCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGCCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACg                                                                                                                                                                                                                                           >  1:165196/1‑251 (MQ=255)    agcCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGa                                                                                                                                                                                                                                        <  2:20604/251‑1 (MQ=255)    agcCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATAGACAGTACAAAACGGGa                                                                                                                                                                                                                                        <  2:304112/251‑1 (MQ=255)     gccgccATCGGTCCTGGCCCCAGCTCGCGCAGACAGGTCAGTGGGTGGTAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCGTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGaa                                                                                                                                                                                                                                       <  2:262753/251‑1 (MQ=255)            tCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGc                                                                                                                                                                                                                                  >  1:226013/1‑249 (MQ=255)                    ggCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGATGGTCGCGCGCCGACTTCCATGTCCAATGGTTGAACAAGGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAATGTCAGGATCAAGCCCTGGAAGGTCCTGGGATCAAACTGTGGCATATTATGTCGTGCTGGATACTTGTTGATTTAAAGGAAGGGATTAGTATACCCTCTGGAGGGAAGAGGTACAGTGCAAGACGGGAAAAAGCAGTGCTTAAc                                                                                                                                                                                                                        <  2:189721/251‑1 (MQ=255)                    ggCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTaa                                                                                                                                                                                                                         <  2:89422/250‑1 (MQ=255)                                              tGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGcc                                                                                                                                                                                               <  2:314906/250‑1 (MQ=255)                                                                  cGACTTGCATGTCCCATGGTTGAACAATGGTGCAGCCCTGGCGAGCACAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCGGGTATCAAACGTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAGCGGGAAAAAGCAGGGCTTAACGCATGGAAAGATTCAAAAAATGGCCATCCGCGTCAAAAGAACAGAc                                                                                                                                                                          <  2:244483/251‑1 (MQ=255)                                                                   gACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCATGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGAc                                                                                                                                                                          >  1:459504/1‑250 (MQ=255)                                                                                 agggTTGAACAATGGTGCAGCCCTGGCGATCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGTAGTTCCTGGTATCAAACTTTTGCATATTATGTCGTGCTTGAGACGTGTGTATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAATAGATACAGTGCAAATCGTGTAAAAGCAGGGCTTAATGCATGGGAAGATGTAAAAAATGGCCGTCCTCGTCAAAAGAACAGACAGACGGCTCTTTACg                                                                                                                                                           <  2:194692/249‑1 (MQ=255)                                                                                   ggTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAATGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACgg                                                                                                                                                          <  1:41934/250‑1 (MQ=255)                                                                                        aaCAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGt                                                                                                                                                     >  1:344469/1‑250 (MQ=255)                                                                                        aaCAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTAAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCttttatgccgact                                                                                                                                                    >  1:421523/1‑241 (MQ=255)                                                                                             tGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAAcc                                                                                                                                               >  2:248263/1‑251 (MQ=255)                                                                                                 gCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCgcgc                                                                                                                                           <  2:438604/251‑1 (MQ=255)                                                                                                   ggCCCTGGCGAGCCCAGTAATCCTGTAAGTGCCGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTTGAGTTAAAGGAAGGGATCAGTATACCCTCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCGTTACGGAAGGTATAACCGCGCAt                                                                                                                                         <  2:404795/250‑1 (MQ=255)                                                                                                    gCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGAGATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCAtt                                                                                                                                        <  1:212051/251‑1 (MQ=255)                                                                                                         ggCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTGCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGAGACGTGTGGATTTAAAGGAAGGGATCAGTATGCCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGAGGCAAAAAATGGCCATCCGCGTCAAAATAACATACAAACTGCTCGGTACGGAAGGTATAGCCGGGGATTTCAt                                                                                                                                    <  2:289990/250‑1 (MQ=255)                                                                                                         ggCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATa                                                                                                                                   <  2:137545/251‑1 (MQ=255)                                                                                                         ggCGAGCCCAGTAATCCTGTAAGGACAGGATCAAGCCCTGGAAGGTCCTGGCATCAAACTTTTGAATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCCTGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCAt                                                                                                                                    >  2:55502/1‑250 (MQ=255)                                                                                                                ccAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTccc                                                                                                                            >  1:447740/1‑251 (MQ=255)                                                                                                                 cAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCt                                                                                                                           >  1:13954/1‑251 (MQ=255)                                                                                                                              aGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAAATTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTAAGGAAGGAATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGa                                                                                                               >  1:254360/1‑250 (MQ=255)                                                                                                                                       acAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGAc                                                                                                     <  1:314755/250‑1 (MQ=255)                                                                                                                                                ggAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGAtttt                                                                                            <  1:335500/251‑1 (MQ=255)                                                                                                                                                 gAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATTCGTGTGGATTTAAAGGAAGGGAACAGTATACCCGCTGGAGGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGGTTAACGCATGGGAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACGGCTCTTTACGGAAGGGATAACCGCGCATTTCATAGCTTTCCTGGAACTGTTCGAAGGCGGTGACGGCGAttttt                                                                                           <  2:410386/251‑1 (MQ=255)                                                                                                                                                               cAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTTCCTGGAACTGTTCGAAGGCGGTTACGACGATTTTTTGTTTGCCGgtgt                                                                              <  2:153788/250‑1 (MQ=255)                                                                                                                                                                        gCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGtt                                                                     <  1:248263/250‑1 (MQ=255)                                                                                                                                                                               attTCGTGCTGGATACGTGTGGATTTAAAGGACGGGATCAGTATCCCCGCTGCATGGAAGATATAAAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATCGCTTCCCTGGAACTGTTCGCAGGCGGTGACGTAGATTTTTTGCTCGCCGGTGTTATAACGTTTTgccgcc                                                             >  2:293490/1‑251 (MQ=255)                                                                                                                                                                                     ggCTGGATACGTGTGGATTTAAAGGAAGGGATCAGGATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGGCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTAGATCCGCGCAGTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGAAGTTGTGCTCGCCGGTGGTATGACGTTTTGCCGCCTGATcc                                                       <  1:408282/250‑1 (MQ=255)                                                                                                                                                                                      gCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCGTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCTGTGTTATAACGTTTTGCCGCCTGATCCt                                                      <  2:447740/251‑1 (MQ=255)                                                                                                                                                                                                  tgGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCat                                          >  1:271776‑M1/1‑249 (MQ=255)                                                                                                                                                                                                     aTTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAAcc                                                                                                                                               >  1:316644/1‑147 (MQ=255)                                                                                                                                                                                                     aTTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATAGACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAAcc                                                                                                                                               <  2:316644/147‑1 (MQ=255)                                                                                                                                                                                                                  gATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGACATCAGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGATTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacg                          >  2:1297‑M1/1‑233 (MQ=255)                                                                                                                                                                                                                    tCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGCAAGATGCAAAAAATGGCCATACGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGCTTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtt                        >  2:347989‑M1/1‑231 (MQ=255)                                                                                                                                                                                                                      aGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAAc                                                                                                                      <  1:354851/155‑1 (MQ=255)                                                                                                                                                                                                                      aGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAAc                                                                                                                      >  2:354851/1‑155 (MQ=255)                                                                                                                                                                                                                         ataCCCGCTGGATGGAAGATATACAGTACACAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGcc                                                                                   >  2:208841/1‑187 (MQ=255)                                                                                                                                                                                                                         ataCCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAAGGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTGCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGcc                                                                                   <  1:208841/187‑1 (MQ=255)                                                                                                                                                                                                                            cccGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtg                >  1:125099‑M1/1‑223 (MQ=255)                                                                                                                                                                                                                              cGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTAAGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgtt              >  2:243098‑M1/1‑221 (MQ=255)                                                                                                                                                                                                                                cTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgt            >  2:371722‑M1/1‑219 (MQ=255)                                                                                                                                                                                                                                       gaAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttggg     >  2:381776‑M1/1‑212 (MQ=255)                                                                                                                                                                                                                                       gaAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttggg     >  2:174154‑M1/1‑212 (MQ=255)                                                                                                                                                                                                                                          gATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctg  >  2:72404‑M1/1‑209 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               AGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTG  >  NC_000913/3725148‑3725631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑