Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,768	C→T	W362* (TGG→TGA)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,768	0	C	T	100.0%	190.6 / NA	54	W362* (TGG→TGA)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/19);  total (35/19)

GCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTC  >  NC_000913/3468522‑3469004                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             gCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTGCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAgg                                                                                                                                                                                                                                          <  1:191335/251‑1 (MQ=255)          gTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGt                                                                                                                                                                                                                                 <  1:13504/251‑1 (MQ=255)                   gACAGAAGTGTTATAATGTGCATCGTTATAATCTACGATGCGGTCCGCGCGGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTAc                                                                                                                                                                                                                        <  2:59099/251‑1 (MQ=255)                      aGAAGTGTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCAt                                                                                                                                                                                                                     <  1:232086/251‑1 (MQ=255)                                       cATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTaa                                                                                                                                                                                                    <  2:127712/251‑1 (MQ=255)                                        aTCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCACCAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTCGCTGTACGCTCGAGAGGCCATGGGTTATTGGAGTTTTCACCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTAc                                                                                                                                                                                                                        >  1:250236/1‑230 (MQ=255)                                                      cGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATACGCAAAAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACAGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGATATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTcc                                                                                                                                                                                     >  1:303678/1‑251 (MQ=255)                                                                 gcgcTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACCTTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTcc                                                                                                                                                                           >  2:139143/1‑250 (MQ=255)                                                                        ccGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTg                                                                                                                                                                    <  1:145410/249‑1 (MQ=255)                                                                             ttGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAAt                                                                                                                                                                <  1:156011/249‑1 (MQ=255)                                                                               gctgcacttgaacccacccGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGAGTCAATTCCGCAGCGGTAGCTGTACGCCCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTTCCCCGTGGGCAATCTTTAGACCCAGCCCATCAGGCGTTCTTGTAAACATTGCCACTGAATATAACATGGTAAATTACGCTGCCTTCCTGGCCGCTCCACGCTTACAATGAGt                                                                                                                                                            <  2:156643/234‑1 (MQ=255)                                                                                      tGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGtt                                                                                                                                                     >  1:8714/1‑251 (MQ=255)                                                                                       ggAAACCATCCGCAACAATGACGCCGCCGTTATCAATTACACAGGTAGTCGCGCTACCGTACTGACAAAATTCCGCAGCGGTAGCTGTACGCTCGCGAAGCCATGGGTTATTGGAGTTATCAGCGATGGTACCCCGTGGGCAATCTTTAGAACCAACCCATCAGGCGTTCTTGTAAACATTACCATTGACAATAACATGGTCAATTTCGCTACCTTCCTGGCCGCTCAACGCTTGCAATGAGTAATCGtt                                                                                                                                                     >  1:327896/1‑250 (MQ=255)                                                                                           cccATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCTTACTGACTCAAGTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGc                                                                                                                                                <  2:46369/250‑1 (MQ=255)                                                                                            ccttCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACGCAAGTCCGCAGCGGTAGCTGTACTCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCATCCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTGCTCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCt                                                                                                                                               <  2:396394/248‑1 (MQ=255)                                                                                                  gCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCCTGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGc                                                                                                                                         >  2:117905/1‑251 (MQ=255)                                                                                                    aacaaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGCGTAATCGTTAACGCTATCCGCTg                                                                                                                                       >  2:332028/1‑251 (MQ=255)                                                                                                      caaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTggg                                                                                                                                     >  2:48629/1‑250 (MQ=255)                                                                                                                  cgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCg                                                                                                                         >  2:129912/1‑251 (MQ=255)                                                                                                                         aaTTTCACAGGAAGTGCGGTTACCGCACTGACCCAACTCCGCAGCGGCCGCTGTACGATCGAGACGCCCTGGGTTCTTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCACCCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAACTTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTAt                                                                                                                  >  2:38689/1‑251 (MQ=255)                                                                                                                                      gTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTggggg                                                                                                     >  2:338335/1‑250 (MQ=255)                                                                                                                                              aCCGTACTGAATCACTTCCGACGCGGTAGCTGTAAGCCCGCGCCGCCATGGGTTATTGGAGTTTTAAGAGCTGGCACCACGTGGGCAATCTTTAGACCCACCCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACCTGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGTGGTCACCGgtg                                                                                             >  2:442406/1‑251 (MQ=255)                                                                                                                                                 gTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTGTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCGCCACGCTTGCAATGATTAATCGTTAACGCTATCCGCTGTTTTTGATGGCTCGCTGTTATCCGGAGTGGGGGTTACCGGTGttt                                                                                          <  2:303678/251‑1 (MQ=255)                                                                                                                                                  tACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGACATGGGTTATTGGAGTTTTCACCGATGAAACCAAGTGCGCAATCTTTAGACCCAACCCATCCGGCGTTCTTGTAAACATTACCCTTGAACATAACATGGTAAATTTCGCTACCTTCCTGGCAGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTc                                                                                         >  1:189071/1‑251 (MQ=255)                                                                                                                                                   aCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTCAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCACTGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTACCCGGTGTTTcc                                                                                        >  2:139632/1‑251 (MQ=255)                                                                                                                                                         tCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGtt                                                                                  >  1:38288/1‑251 (MQ=255)                                                                                                                                                          cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTTTAAACATTACCATTGAAAAt                                                                                                                                                                                                              <  2:149394/126‑1 (MQ=255)                                                                                                                                                          cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAAt                                                                                                                                                                                                              >  1:149394/1‑126 (MQ=255)                                                                                                                                                          cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGCAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTTCCGTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGtt                                                                                  <  1:197395/250‑1 (MQ=255)                                                                                                                                                            aTTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTgg                                                                                >  1:396548/1‑250 (MQ=255)                                                                                                                                                                   aGCGGTAGCTGCGCGCTCGACACGCCACGGGACTTTGCAGGTTTCAGTGATGGTACCCCGTGCGCAATCCTTAGACCCAACCCATCAGGCGTTCTTGTAAGCATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCCTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGCTGATGGCACGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATAAACCg                                                                        >  2:23917/1‑251 (MQ=255)                                                                                                                                                                     cGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGa                                                                      <  1:123872/251‑1 (MQ=255)                                                                                                                                                                            tGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATTAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTcac                                                                 >  2:3583/1‑249 (MQ=255)                                                                                                                                                                               aCGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCAc                                                            >  2:134194/1‑251 (MQ=255)                                                                                                                                                                               aCGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCAc                                                            >  2:110481/1‑251 (MQ=255)                                                                                                                                                                               aCGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCAc                                                            >  1:332231/1‑251 (MQ=255)                                                                                                                                                                                    cGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGcc                                                        <  1:196738/250‑1 (MQ=255)                                                                                                                                                                                           ccATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTCAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATtattat                                                >  1:197650/1‑251 (MQ=255)                                                                                                                                                                                           ccATGGGTTATTGGAGTTTTCAGCGCTGGTACCCAGTGGGCAATCTTTCGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGACAATAACATGGTACATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGCGTTGGATCAACCGGAGTCACAGCACCGCCATtattat                                                >  2:444950/1‑251 (MQ=255)                                                                                                                                                                                            cATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATc                                               >  1:11141/1‑251 (MQ=255)                                                                                                                                                                                            cATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATc                                               >  1:323149/1‑251 (MQ=255)                                                                                                                                                                                                         aGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGa                                   >  1:40092/1‑250 (MQ=255)                                                                                                                                                                                                                aGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGc                           >  2:228503/1‑251 (MQ=255)                                                                                                                                                                                                                      ggTACCCCGTGGGAAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCCCGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGt                     >  2:475386/1‑251 (MQ=255)                                                                                                                                                                                                                       gtcccccGTGGGCAATCTTAAGACCCCACCCATCAGGCTTTCTTGTAAACATTACCATTGAAAAAAACATGGTAAATTTCGCTCCCTCCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGAGGGCTCGCTGTTACCGGGAGTGGGGGTCACGGGTGTTCCCGGAGTGGGATCACCCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGtt                    <  1:139143/248‑1 (MQ=255)                                                                                                                                                                                                                        tACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGtt                                                                                                                                  <  1:145540/140‑1 (MQ=255)                                                                                                                                                                                                                        tACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGtt                                                                                                                                  >  2:145540/1‑140 (MQ=255)                                                                                                                                                                                                                                 ggCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTgg          >  2:2556/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaTCTTAAGACCCAACCCATGAGGCTTTCTTGAAAACATTCCCTTTAAAAATAACATGGTAAATTTCTCTCCCTCCCTGGCCCCCCCCCGCTTGCAATGAGTAATCGTTAACGCTATCCGTGGGTTGTGAGGCCCCGGTGTTATCCGGAGTTGGGGTCCCCGGTTTTTCCGGAGTGGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTg       <  1:228503/251‑1 (MQ=255)                                                                                                                                                                                                                                     aTCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTACATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTgc      >  2:283064/1‑250 (MQ=255)                                                                                                                                                                                                                                       cTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGAtt    >  2:360648/1‑251 (MQ=255)                                                                                                                                                                                                                                         ttAGACCCACCCCATCAGGCGTTCTTGTCCACATTACCATTGAAAATAACATGGTACATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCCCCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTc  >  2:290384/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cccaTCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCAc                                                            <  2:340026/183‑1 (MQ=255)                                                                                                                                                                                                                                                   cccaTCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCAc                                                            >  1:340026/1‑183 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             GCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTC  >  NC_000913/3468522‑3469004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑