Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,959	T→C	D236G (GAT→GGT)	agaR ←	DNA‑binding transcriptional repressor AgaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,959	0	T	C	100.0%	259.9 / NA	70	D236G (GAT→GGT)	agaR	DNA‑binding transcriptional repressor AgaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/25);  total (45/25)

CCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCGACTGACGGCGCAAATGCCCGCCG  >  NC_000913/3277713‑3278201                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   ccTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAAt                                                                                                                                                                                                                                                >  1:260242/1‑251 (MQ=255)    ataCCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCAGGTCGATACGTTGAGTACCAATGa                                                                                                                                                                                                                                              <  2:200972/250‑1 (MQ=255)        cGTATCCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACGGGGAAACCCACCCCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAGTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCATCCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCATCATCAACAAGGATCATGGCGATACGTGGAGTACCAATTATCTTa                                                                                                                                                                                                                                         <  2:198264/251‑1 (MQ=255)                 cAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGCTACGTTGAGTACCAATGATCTTATGTAAACTg                                                                                                                                                                                                                                >  2:394112/1‑251 (MQ=255)                 cAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTg                                                                                                                                                                                                                                >  1:360290/1‑251 (MQ=255)                  aaaaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCAGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTgg                                                                                                                                                                                                                               >  1:228381/1‑251 (MQ=255)                     aTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAAc                                                                                                                                                                                                                            >  1:225003/1‑251 (MQ=255)                      ggTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACg                                                                                                                                                                                                                           <  1:369850/250‑1 (MQ=255)                        ttAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGAt                                                                                                                                                                                                                         >  1:482404/1‑251 (MQ=255)                          aaaaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTg                                                                                                                                                                                                                       >  2:332946/1‑251 (MQ=255)                                 ggACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGAATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTAc                                                                                                                                                                                                                >  1:86689/1‑251 (MQ=255)                                        ctcctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATc                                                                                                                                                                                                         >  1:281964/1‑251 (MQ=255)                                          cctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATc                                                                                                                                                                                                         <  1:332946/249‑1 (MQ=255)                                                       aaaCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACgatgat                                                                                                                                                                                          >  1:276596/1‑251 (MQ=255)                                                            cTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGtt                                                                                                                                                                                     >  1:351153/1‑251 (MQ=255)                                                              cACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTcc                                                                                                                                                                                   <  1:122045/251‑1 (MQ=255)                                                                        aCAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGc                                                                                                                                                                         >  1:72816/1‑251 (MQ=255)                                                                        aCAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGc                                                                                                                                                                         >  1:59468/1‑251 (MQ=255)                                                                               aaaCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCg                                                                                                                                                                  >  1:325846/1‑251 (MQ=255)                                                                               aaaCCCATTGATGGGGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTTAGTTCCAATGATCGTATGTAAACTGGGACGAGTGAACGGACTGGAATCGGTGACCACGAGGATCCGGTCCGCAACTTCGCACATCCg                                                                                                                                                                  <  2:475976/251‑1 (MQ=255)                                                                                   ccATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACgg                                                                                                                                                              >  1:378950/1‑251 (MQ=255)                                                                                     cgggagggtgttccaaaggCCCGCCGAACGCAGTCGTGTTGACCAGCGGGGATCCTTGCTCACTCCCCGAACAGAATCACTTCAACCCCAGGCTTTCGCAGGCCTTCCAGGCTATTCGCAGGAATGCTTTCATCAACAATGATCATGTCGATACGTTGAGTCCCAATGATCTGATGTAAACTGTACCGATTGACCGTACGGGAATCGGTGACCACGATGATCCGGTCCGCATCCTGGCACATCCGACGGtt                                                                                                                                                            <  2:44815/233‑1 (MQ=255)                                                                                       tGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGttt                                                                                                                                                           <  2:360290/250‑1 (MQ=255)                                                                                        gATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTa                                                                                                                                                          >  1:92644/1‑250 (MQ=255)                                                                                           ggTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACg                                                                                                                                                      <  2:72816/251‑1 (MQ=255)                                                                                              gtTCACAATGCCCGTCGAACGCATTCGTGTTGACCAGCGGGCCTCCTTTCTCACTCCCCGACCAGAATCACTTCAGCCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCGCACGGTTTAAACGAGc                                                                                                                                                   >  2:186416/1‑251 (MQ=255)                                                                                                   aaaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCTCACATCCGACGGTTTAAACGAGcttcat                                                                                                                                              <  2:226004/250‑1 (MQ=255)                                                                                                             cgAACGCAGTCGTGGTGGCCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtg                                                                                                                                    <  2:5572/251‑1 (MQ=255)                                                                                                              gAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtgt                                                                                                                                   >  1:116560/1‑251 (MQ=255)                                                                                                               aaCGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTAtgtg                                                                                                                                  >  1:319849/1‑251 (MQ=255)                                                                                                                    aTTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGCCCAGAATAACTTCACCCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTg                                                                                                                             >  1:445051/1‑251 (MQ=255)                                                                                                                    aTTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTg                                                                                                                             >  1:133725/1‑251 (MQ=255)                                                                                                                       cGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGGCCAGAATCACTTCAACCCCAGCCTTTCGCAGTCATGCCAGGCTATCCGTAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTCCCAATGATCTGATGTAAGCTGGAACGATTGAACTTACGCGAATCGGTGACCACGATGATCCGTTCCGCAACTGCGCACATCCTAGGGTTTAAACGAGTTTCATCTTCATTATGTGTGCTGAc                                                                                                                           <  1:275326/250‑1 (MQ=255)                                                                                                                                cAGCGGTCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCgcgcccc                                                                                                                 <  2:260242/251‑4 (MQ=255)                                                                                                                                      gCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgtccg                                                                                                           >  2:131307/1‑247 (MQ=255)                                                                                                                                        aTCCTTTCTCACGCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAgatcgat                                                                                                         <  2:183357/251‑1 (MQ=255)                                                                                                                                                       ccGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACTCCAAg                                                                                          <  1:328407/251‑1 (MQ=255)                                                                                                                                                         gACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa                                                                                         >  2:408706/1‑250 (MQ=255)                                                                                                                                                         gACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGa                                                                                         >  2:410946/1‑250 (MQ=255)                                                                                                                                                          aCCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCAGCTACACCAAGaaa                                                                                       <  2:86689/251‑1 (MQ=255)                                                                                                                                                                  cACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCatat                                                                               <  2:116560/251‑1 (MQ=255)                                                                                                                                                                   aCTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATc                                                                              >  1:135982/1‑251 (MQ=255)                                                                                                                                                                         aCCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTg                                                                                                                                                                                                     >  2:383053/1‑126 (MQ=255)                                                                                                                                                                         aCCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTg                                                                                                                                                                                                     <  1:383053/126‑1 (MQ=255)                                                                                                                                                                               gCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAAtt                                                                  <  2:351153/251‑1 (MQ=255)                                                                                                                                                                                      gCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGCTCTTATGTAAACTGCAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAGCTGCGCACATCCGACGGTTTAAACGAGCTTCATCTGCATGATGTGTGCTGACGCCGCGCTCCAGATCGAGCGCATCTACACCAAGAAACAGCACATCGAAGTGGTAATTTTGCAGc                                                           <  1:208918/251‑1 (MQ=255)                                                                                                                                                                                              tCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTc                                                   >  1:287526/1‑251 (MQ=255)                                                                                                                                                                                               ccAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTc                                                   >  2:98179/1‑250 (MQ=255)                                                                                                                                                                                                    cTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTg                                             >  1:471377/1‑251 (MQ=255)                                                                                                                                                                                                     tATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCACTGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCACCTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTg                                             >  1:202840/1‑250 (MQ=255)                                                                                                                                                                                                      aTCCGCAAGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTg                                             <  2:482404/249‑1 (MQ=255)                                                                                                                                                                                                        ccGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATc                                          <  1:408706/250‑1 (MQ=255)                                                                                                                                                                                                         cGCAGGAATGCCTTCATCAACAATGAACATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGc                                        <  2:418511/251‑1 (MQ=255)                                                                                                                                                                                                           cAGGAATGCCTTCATCAACAATGATCATGTAGATACGTTGAGTACCACTGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCg                                      >  1:48548/1‑251 (MQ=255)                                                                                                                                                                                                            aGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGt                                     >  2:394234/1‑251 (MQ=255)                                                                                                                                                                                                            aGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGt                                     >  2:371595/1‑251 (MQ=255)                                                                                                                                                                                                             ggAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGt                                     >  1:254600/1‑250 (MQ=255)                                                                                                                                                                                                              gAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTACCACTGCCCTTACGTAAACTCGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTa                                    >  1:29775/1‑250 (MQ=255)                                                                                                                                                                                                                    cTTCATCAACAATGATCATGACGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCACCTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGACGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTaaaa                                 >  1:183223/1‑247 (MQ=255)                                                                                                                                                                                                                        atcaACACTAATCATGTGGATACGTTGAGTACCAATGATCTTATGAAAACTGTCACGATTGAACTTACTAGAATCGGTGACAACGCTGATCCGTTCCGCAACTTCGCACATCCGACGGTTTCAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTaaaaatcttgcg                         >  2:289856/1‑244 (MQ=255)                                                                                                                                                                                                                        atcaACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCg                         >  1:164262/1‑251 (MQ=255)                                                                                                                                                                                                                         tcaACAATGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCg                         >  2:128746/1‑250 (MQ=255)                                                                                                                                                                                                                           aacaaTGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCGACt                      >  2:392813/1‑251 (MQ=255)                                                                                                                                                                                                                            acaaTGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCGACTg                     >  2:320937/1‑251 (MQ=255)                                                                                                                                                                                                                            acaaTGATCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATAGCGAAGTGGTAATGTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCGACTg                     <  2:202840/251‑1 (MQ=255)                                                                                                                                                                                                                                 gATCATGCCGATACGTTGTGTACCAATAATCTTAGGTAAACTTGAACGATGTAACTGACGTTAATCGGTGACCACGATGATCCTTTCCGCAACTTCGCACATCCGACGGTTTAAACCATCTTCTGCTTCAGGATGTGTGCTGACGCCTCGCTCCAGATCTATCGCATCTACGCCAAGAAGCAGCATATCAAAGTTGAAAGTTTGCAGCGATGGCTCAGCCTGATCGcctttaaaaaattacgctgaactgc                <  2:312069/251‑24 (MQ=255)                                                                                                                                                                                                                                   tCATGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCACGAAACAGCATATCGAAGTGGTAATTTTGCAGCGCTTGCTCAGCCTGATCGCCGTAAAAAGATTGCGACTGACGgcgc              >  2:371965/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCGACTGACGGCGCa             >  2:279990/1‑250 (MQ=255)                                                                                                                                                                                                                                     aTGTCGATACGTTGAGTACCAATGATCTTATGTAAACTGAAACGATTACACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCCTTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCCAGAAACAGCATATCGCAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCGACTGACGGCGCa             >  1:42616/1‑250 (MQ=255)                                                                                                                                                                                                                                               gTTGAGTACAAATGATGTTCTGTACCCTGGAAAGATTGAACTTACTGGACTCGGTGCCCACGATGATCCGTTCCGCAACTTCGCAAATCCGACGGTTTAAACGAGCTTAATCTCCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCGACTGACGGCGCAATTGCccgccg  >  2:285651/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGGAATCGGTGACCACGATGATCCGTTCCGCAACTTCGCACATCCGACGGTTTAAACGAGCTTCATCTTCATTATGTGTGCTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAACAGCATATCGAAGTGGTAATTTTGCAGCGATTGCTCAGCCTGATCGCCGTAAAAAGATTGCGACTGACGGCGCAAATGCCCGCCG  >  NC_000913/3277713‑3278201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑