Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,243,185	C→T	T156T (ACG→ACA)	folE ←	GTP cyclohydrolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,243,185	0	C	T	97.6%	142.8 / NA	42	T156T (ACG→ACA)	folE	GTP cyclohydrolase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/22);  minor base A (1/0);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATT  >  NC_000913/2242939‑2243431                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCTCAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACTCAGTAATGCACCGCGTCGATCGAGACAGCCTCGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCa                                                                                                                                                                                                                                                    <  1:208136‑M2/245‑1 (MQ=255)  cataaTCGCGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGGCGTTGTCGCGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTc                                                                                                                                                                                                                                                     >  1:350050‑M2/6‑249 (MQ=255)     aatcaggCGTGACGTTGCGCTCCATTGTTCCTGACTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGAc                                                                                                                                                                                                                                                 <  2:137727/244‑1 (MQ=255)         gAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTcc                                                                                                                                                                                                                                            >  1:338231/1‑251 (MQ=255)          ggCGTGGCGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGAGGACGCACAGGGCGCAGAAACTCGGGGCGCGTATTCGGACGGGATGTGAGCAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCAACTCGGATGCCACGTTCCTGCACGCAGTAATGGACCGCGTCGATCGAGACAGCCTCGTTATTGGTGCCCACCAGGTTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCt                                                                                                                                                                                                                                           <  1:60545/250‑1 (MQ=255)             gTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGGTGTGAGGACGCGCAGCGCGCAGAAGCTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCACTAAGAATTTGCTGTGTCAGACGTTCCTGCa                                                                                                                                                                                                                                        <  2:8994/251‑1 (MQ=255)                  gTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGc                                                                                                                                                                                                                                   >  1:390510/1‑251 (MQ=255)                    tGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCg                                                                                                                                                                                                                                  <  2:365960/250‑1 (MQ=255)                             ttgTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGc                                                                                                                                                                                                                        >  1:335972/3‑251 (MQ=255)                              tgTTCCTTCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGTCACTGGTTGCATCGCGGATGCCACGCTCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGTGGCCCATCAGCGGTTGTAGCGCAATAAGAATTTGCTTTGGCAGACGGTCCTGCACCTGCGGACGCTGGGc                                                                                                                                                                                                                        <  2:193176/249‑1 (MQ=255)                               gTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGCGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCa                                                                                                                                                                                                                       >  1:320875/1‑250 (MQ=255)                                        ttttAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGAATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTCATGCACCGCGTAGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGc                                                                                                                                                                                                              >  1:73106/1‑250 (MQ=255)                                            aaTCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAAt                                                                                                                                                                                                         <  1:348094/251‑1 (MQ=255)                                                      gatgaCGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAAtt                                                                                                                                                                                               <  2:69916/251‑1 (MQ=255)                                                      gatgaCGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAAtt                                                                                                                                                                                               <  1:324240/251‑1 (MQ=255)                                                                                       tATTCTGACTGGATTTGAACAATCCACCAAGAGAGATGGTTGTCGTGACACTGGTTGCATCGCGGATGCCCAGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGatc                                                                                                                                                              >  2:72354/1‑250 (MQ=255)                                                                                        aTTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGatat                                                                                                                                                             >  2:92332/1‑251 (MQ=255)                                                                                        aTTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGatat                                                                                                                                                             <  1:83528/251‑1 (MQ=255)                                                                                                 ggATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCAc                                                                                                                                                      <  2:402999/249‑1 (MQ=255)                                                                                                        aaCAATCCACCACGAGAGGTAGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGCTCACCGAATCTTTCGGGATATAGGCCACCGTCGCttt                                                                                                                                             >  2:284640/1‑251 (MQ=255)                                                                                                          caaacaaccaAGAGAGGTCGTTGTCGGGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCGCCGCGTCGATCGAGACAGCCTCGGTATTGGTGCCCAGCAGCGTATGTAGCGCAATAAGAATTTGCTGTGTCAGAAGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGGACAATGCTGTTAATTTTTGACAGACCGATCTACGAACCTTTCTGGAGATAGGCGACCGTCGCTTTGc                                                                                                                                           <  2:37207/245‑1 (MQ=255)                                                                                                                                      gCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTcac                                                                                                                >  2:335336/1‑250 (MQ=255)                                                                                                                                        ccgggTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAAGGCACCGCGTAGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAgg                                                                                                             <  2:350050/248‑1 (MQ=255)                                                                                                                                           ggTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGc                                                                                                          >  1:351679/1‑251 (MQ=255)                                                                                                                                                cagcgcgGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTc                                                                                                     <  2:338231/248‑1 (MQ=255)                                                                                                                                                 aTCGCGGATGCCACGCGCCTTCACGCAGTAATGAACCCCGATGATCCAGCCAGCCACGTTATTGGCGCCCAGCAGCGCTTCTAGCGCAATCAGCCTCTGCTGAGCCCGACGTTACTGCCCCTGCGGACGCTGCGCAAAGACCAGCACACTCCGGTTAATTTTTGACAGACCGATCACAGAATCTTGCGGGATAAAGGCCACCGTCGCTTTGCCCTCGATGGTCACAAACTGGCGTTCACAGGTGCTggccc                                                                                                    >  1:446228/1‑248 (MQ=255)                                                                                                                                                   cgcgGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGAACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTc                                                                                                     >  2:370078/1‑248 (MQ=255)                                                                                                                                                      ggATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCt                                                                                                         <  2:166670/241‑1 (MQ=255)                                                                                                                                                      ggATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCt                                                                                                         >  1:166670/1‑241 (MQ=255)                                                                                                                                                          gCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGACACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGatat                                                                                           >  1:220058/1‑251 (MQ=255)                                                                                                                                                           ccACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATc                                                                                          <  1:264744/251‑1 (MQ=255)                                                                                                                                                                cgcCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCa                                                                                     <  2:335972/251‑1 (MQ=255)                                                                                                                                                                     tCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGt                                                                                 <  1:290797/250‑1 (MQ=255)                                                                                                                                                                       aCGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGAc                                                                              >  2:182019/1‑251 (MQ=255)                                                                                                                                                                              aaTGCACCGCGTCGATCGAGACAGCCACGTTAGTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACTGTGACCATTTCa                                                                       <  1:107228/251‑1 (MQ=255)                                                                                                                                                                                               gagaCAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGtt                                                      <  1:52843/251‑1 (MQ=255)                                                                                                                                                                                                     gCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAAt                                                <  2:351679/251‑1 (MQ=255)                                                                                                                                                                                                     gCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAAt                                                <  2:95310/251‑1 (MQ=255)                                                                                                                                                                                                          gTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAggg                                           >  1:116239/1‑251 (MQ=255)                                                                                                                                                                                                                ggTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGAttt                                     >  2:95335/1‑251 (MQ=255)                                                                                                                                                                                                                 gTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGAGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGAGAtttt                                    >  2:466289/1‑251 (MQ=255)                                                                                                                                                                                                                                               aTTTGATGTGTCAGAAGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCAAACTGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACCGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGgagc       >  2:43566/1‑249 (MQ=255)                                                                                                                                                                                                                                                   gctgtGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAAtt  <  1:379229/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCTGGGCAAAGAACTGCACAATGCGGTTAATTTTTGACAGACCGATCACCGAATCTTTCGGGATATAGGCCACCGTCGCTTTGCCATCGATGGTAACAAAATGGTGTTCACAGGTGCTGGTCAGAGTGATATCGCGCACGGTGACCATTTCATCGACCTTCATTTTGTTTTCAATGAGGGTGATTTTCGGGAAATTGGCGTAATCCAGACCGGAGAAAATT  >  NC_000913/2242939‑2243431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑