Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,242,968	G→T	intergenic (‑1/+16)	yeiB ← / ← folE	DUF418 domain‑containing protein YeiB/GTP cyclohydrolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,242,968	0	G	T	100.0%	119.9 / NA	34	intergenic (‑1/+16)	yeiB/folE	DUF418 domain‑containing protein YeiB/GTP cyclohydrolase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/15);  total (19/15)

AGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCG  >  NC_000913/2242750‑2243207                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                cggtatctctgaaggcatggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCTTGTTAATCAGTTGTGATGACGc                                                                                                                                                                                                                  <  1:340928‑M2/55‑1 (MQ=255)  ggtatctctgaaggcatggtgatggctgccggtcctggcgggaaagctattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCCTTGTTCCTGCCTTTTAATCAGTTGTGATGAcgccc                                                                                                                                                                                                                >  2:83528‑M2/195‑249 (MQ=255)  ggtatctctgaaggcatggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGcac                                                                                                                                                                                                                <  2:381466‑M2/57‑1 (MQ=255)    tatctctgaaggcatggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgacggtgctaaaacgggtaatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgagtttgtctctctctaacccgcataaTCGAGCGTGACGGTACTCTCCATTGTTTCTGCCTTTTAATCAGTTGTTATGAcgcaccg                                                                                                                                                                                                              >  2:348094‑M2/193‑249 (MQ=255)                   ggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttttttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgaataaTCGAGCGTGACGTTGCGCTCCCTTGTTCCTGCCTTTTAATCAGTTGTTATGACGCACAGCGCGCAGAaacttgt                                                                                                                                                                                               >  1:231648‑M2/178‑248 (MQ=255)                         ggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGcgcg                                                                                                                                                                                         <  2:86102‑M2/80‑1 (MQ=255)                                              agatattttcctgctaagcccggatgccggtgctaaaacgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAAc                                                                                                                                                                     >  1:276551‑M2/151‑250 (MQ=255)                                                                                                             cttcaaggagctgaatcgacagcgactattctatataaattactaaagttgttatcttgagattttgactctatctaaccagcataaTCGAGCGTGACGTTGCGCTCAATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAACCTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGCTGCCACGCGCCTTCACGc                                                                                                     >  2:243679‑M2/88‑251 (MQ=255)                                                                                                                   ggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAAt                                                                                               >  1:402999‑M2/82‑251 (MQ=255)                                                                                                                         gcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACcg                                                                                         <  2:244980‑M2/176‑1 (MQ=255)                                                                                                                          catcgacagcgccttttctttataaattcctaaagttgttttcgtgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGTTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACcgc                                                                                        <  1:319018‑M2/177‑1 (MQ=255)                                                                                                                                       ttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGAc                                                                            >  1:98999‑M2/62‑250 (MQ=255)                                                                                                                                              tataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCAc                                                                                                                 <  2:389876‑M2/152‑1 (MQ=255)                                                                                                                                              tataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCAc                                                                                                                 >  1:389876‑M2/55‑206 (MQ=255)                                                                                                                                              tataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATTCACGGCGTCGATCGAGACAGCCACg                                                                     <  1:136460‑M2/196‑1 (MQ=255)                                                                                                                                                   attcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATACACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTg                                                               >  1:479644‑M2/50‑251 (MQ=255)                                                                                                                                                       ctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGc                                                           >  2:379229‑M2/46‑251 (MQ=255)                                                                                                                                                          aagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCca                                                        <  2:351575‑M2/209‑1 (MQ=255)                                                                                                                                                                   tcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGtt                                                <  1:52362‑M2/217‑1 (MQ=255)                                                                                                                                                                    cttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGTATGCCACGCGCCTGCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGccc                                                         <  2:435218‑M2/208‑1 (MQ=255)                                                                                                                                                                    cttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGATCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCACGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGccc                                                         >  1:435218‑M2/33‑240 (MQ=255)                                                                                                                                                                       gcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGt                                             >  2:15234‑M2/30‑249 (MQ=255)                                                                                                                                                                        cgattttgtctctctctcacccgcataaTAGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAgc                                          >  1:388382‑M2/29‑251 (MQ=255)                                                                                                                                                                          attttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGGGCCCAGCAGCGTTTGTAgcgc                                        >  2:264744‑M2/27‑251 (MQ=255)                                                                                                                                                                           ttttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAgcgc                                        >  1:95310‑M2/26‑250 (MQ=255)                                                                                                                                                                               gtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATa                                    >  1:198105‑M2/22‑250 (MQ=255)                                                                                                                                                                                              gcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCTCAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACTCAGTAATGCACCGCGTCGATCGAGACAGCCTCGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCa                    <  1:208136‑M2/245‑1 (MQ=255)                                                                                                                                                                                               cataaTCGCGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGGCGTTGTCGCGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTc                     >  1:350050‑M2/6‑249 (MQ=255)                                                                                                                                                                                                  aatcaggCGTGACGTTGCGCTCCATTGTTCCTGACTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGAc                 <  2:137727/244‑1 (MQ=255)                                                                                                                                                                                                      gAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTcc            >  1:338231/1‑251 (MQ=255)                                                                                                                                                                                                       ggCGTGGCGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGAGGACGCACAGGGCGCAGAAACTCGGGGCGCGTATTCGGACGGGATGTGAGCAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCAACTCGGATGCCACGTTCCTGCACGCAGTAATGGACCGCGTCGATCGAGACAGCCTCGTTATTGGTGCCCACCAGGTTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCt           <  1:60545/250‑1 (MQ=255)                                                                                                                                                                                                          gTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGGTGTGAGGACGCGCAGCGCGCAGAAGCTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCACTAAGAATTTGCTGTGTCAGACGTTCCTGCa        <  2:8994/251‑1 (MQ=255)                                                                                                                                                                                                               gTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGc   >  1:390510/1‑251 (MQ=255)                                                                                                                                                                                                                 tGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCg  <  2:365960/250‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                AGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCG  >  NC_000913/2242750‑2243207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑