Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,7451.G56.0% 44.3 / 40.7 25coding (400/423 nt)yphAputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (2/9);  new base G (4/10);  total (6/19)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGA  >  NC_000913/2673517‑2673991
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
gctattagtggggTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTTCTCTCGATCGGCGTTa                                                                                                                                                                                                                                  <  2:210300/240‑1 (MQ=255)
                 tatggactggCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGAt                                                                                                                                                                                                                   <  2:373875/241‑1 (MQ=255)
                     gAAGGGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATgcggcg                                                                                                                                                                                                             <  2:114173/251‑1 (MQ=255)
                              gccgcGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTGCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCt                                                                                                                                                                                                    <  1:308898/251‑1 (MQ=255)
                                         aaTCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTGTCTACACGCTGGGTACGGCTGTGATTGGTCTCCATTGCTGGGATATGACCGGCGATGCGTTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTTGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                                                                                          <  1:468775/251‑1 (MQ=255)
                                                        tttCACCCGGCCGCTGGCGGTGCTGGTTAATTTCCTCACGCTGGGTTCGGCGGTGATTGGTCCCCATTTCCGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAg                                                                                                                                                                            <  1:24439/250‑1 (MQ=255)
                                                              ccGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTa                                                                                                                                                                    <  1:153905/251‑1 (MQ=255)
                                                                             gctttttATTTTCTACACGCTGGGTGCGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtct                                                                                                                                                     <  2:273284/247‑1 (MQ=255)
                                                                                                        ggcggTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCt                                                                                                                           >  1:4389/1‑250 (MQ=255)
                                                                                                                               gggATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTc                                                                                                    <  1:351093/251‑1 (MQ=255)
                                                                                                                                         cGGCGATGCGGTTGGGCCAAAGATGATTAATTGCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTTCTCTCGATCGTCGTTAGGAAAGATGCCGGATGCGGCGTTAACGCCTGATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGGCATCGCTGAAGTTGTTGATGCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgat                                                                                         <  2:448836/251‑1 (MQ=255)
                                                                                                                                                gCGGTTGGCCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTAAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGatcat                                                                                   <  1:153398/251‑1 (MQ=255)
                                                                                                                                                     ggggCCAAAAATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGGAATTACCGGGCCGGGGGGCAATTTCTCGCGATCGGCGTTAGGAAAGATGCCGGATTCGGCGTGAACGCCTTATCCGGCATTTAATAAAGTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATGCCCCTGGGGCTAGCGCAATGAGATCACCCCCTTCCGTGCTATGAGGAGCAGCCGgc                                                                              <  2:292319/250‑1 (MQ=255)
                                                                                                                                                                            ggAAGAATGTCGGTATCGCTGGCGGGTTCTTGCAATGGGCAATTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGAGGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcagaca                                                       <  2:127890/251‑1 (MQ=255)
                                                                                                                                                                             gaagaaTGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTACCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCcagacag                                                      <  1:59295/251‑1 (MQ=255)
                                                                                                                                                                                         tATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTTCTCTCGGTCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAAc                                          <  1:20252/250‑1 (MQ=255)
                                                                                                                                                                                            cGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCTGGGGG‑CAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTTGCCGGCAATTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTTATTCCCCTGGTGCTAGCTCAATGAGATCACCCCCTTCCTGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCat                                      <  1:241792/251‑1 (MQ=255)
                                                                                                                                                                                             gCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCat                                      >  1:452553/1‑251 (MQ=255)
                                                                                                                                                                                             gCTGGCGCGTTCGTGCTATTGGCAATTGCCGGGCCGGGGG‑CAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGTAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCata                                     <  1:11199/251‑1 (MQ=255)
                                                                                                                                                                                              cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCat                                      >  2:122306/1‑250 (MQ=255)
                                                                                                                                                                                                       tCTTGCTATTGGAAATTACAGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGCACCCCTTATCCGGCATTTACTACATTACAGCCACTCTACCCGCAAGGACATCTCTGAAGTTGCTGATTCCCCTGGTGCTAGCGCAATGAGAACCCACCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGCAATCAAACGCATATCCTTTATc                            >  2:237399/1‑251 (MQ=255)
                                                                                                                                                                                                                    aaTTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCAct                                                                                                                                                                       <  2:100185/99‑1 (MQ=255)
                                                                                                                                                                                                                    aaTTACCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCAct                                                                                                                                                                       >  1:100185/1‑99 (MQ=255)
                                                                                                                                                                                                                        aCCGGGCCGGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGaaa           <  1:285530/251‑1 (MQ=255)
                                                                                                                                                                                                                               cgggGG‑CAATTTATCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTACTCAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATAATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATg   >  2:476377/1‑251 (MQ=255)
                                                                                                                                                                                                                                ggggg‑AAAGTTCTCCCGTTCGGCGTTAGTAAATATGCCGGAGGCGGCGTGACCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGa  <  2:404283/251‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
GCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTGGGATATGACCGGCGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGG‑CAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGA  >  NC_000913/2673517‑2673991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑