Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,249,3590TC32.3% 63.8 / 52.1 62D87G (GAC→GGC) yeiEDNA‑binding transcriptional regulator YeiE
Reads supporting (aligned to +/- strand):  ref base T (21/21);  new base C (12/8);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-02
Rejected as consensus: Frequency below/above cutoff threshold.

CGGCGCGGCGAAAACCACCAGCTCGTCTTCCAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGG  >  NC_000913/2249112‑2249608
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
cggcacggcgAAAACCACCAGCTCGTCTTCAAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTCCCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCtt                                                                                                                                                                                                                                                        <  1:255775/251‑1 (MQ=255)
 ggcgcggcgAAAACCACCAGCTCGTCTTCCAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTc                                                                                                                                                                                                                                                       <  1:31454/251‑1 (MQ=255)
             accaccAGCTCGTCTTCCAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAgttg                                                                                                                                                                                                                                           >  1:38410/1‑251 (MQ=255)
               caccaGCTCGTCTTCCAGCCACGGTTCAGAAATGCTTTCAGTGCTGTGGCACGGTCCTTCAATACCGCC‑AATATCAACGCGGAAATCCCGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATTGTACTACTGGCATAGATACGAATCGCGCCGTTGCCTTCGCGAAACAgttgtt                                                                                                                                                                                                                                         >  2:150306/1‑251 (MQ=255)
               caccaGCTCGTCTTCCAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGCCTTCGCGAAACAgttgtt                                                                                                                                                                                                                                         <  1:384914/251‑1 (MQ=255)
                cccaGCTCGTCTTCCAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGCCTTCGCGAAACAGTTGTTc                                                                                                                                                                                                                                        <  1:219753/250‑1 (MQ=255)
                accaGCTCGTCTTCCAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGCAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTc                                                                                                                                                                                                                                        >  1:237442/1‑251 (MQ=255)
                       cGTCTTCCAGCCACGGTTCAGAAATGAGTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCa                                                                                                                                                                                                                                 <  2:140616/251‑1 (MQ=255)
                          cTTCCAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAAcc                                                                                                                                                                                                                              >  1:126777/1‑251 (MQ=255)
                             ccAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAAGCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTGCGCGAAACAGTTTTTCGATTTCAACCGcc                                                                                                                                                                                                                           <  1:167507/251‑1 (MQ=255)
                              cAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCt                                                                                                                                                                                                                          >  1:410084/1‑251 (MQ=255)
                               aGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTg                                                                                                                                                                                                                         >  1:170926/1‑251 (MQ=255)
                                       ttCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGc                                                                                                                                                                                                                  >  2:13990/1‑250 (MQ=255)
                                                taTTTCAGTGTTGTGCCACGGTCTTTCAAAAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACTTCCTGTTTATTCCCAACGCTAAGTT‑CAATCGGCAACGGCGGATAACCATGGCGAAAACGGGCGATAACTGCAGGCAGAATGTAGTTCCCGATGGTACTACTGCCATAGATACGATTCGCGCCGTTGCCTCCGCGAAACATTTGTTCGTTTCCAACCGCCTGTTCAAGCAATGCCAac                                                                                                                                                                                                         <  1:205828/249‑1 (MQ=255)
                                                  tttaggTGCTGGGGCACGG‑CCCTAAAAAAAGCCCAATATAAACGGGAAAATCCAGCCCCGCTTGCATCACGTCCTGGCTATCCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAAAAGGTATTTCCCGAGGGTACTACGGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAacgc                                                                                                                                                                                                       <  2:410084/245‑1 (MQ=255)
                                                       gTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCACTGCCAACGCACGCgg                                                                                                                                                                                                 >  2:103796/1‑251 (MQ=255)
                                                         gcgtgggcccgttctttaaaaaaaccc‑AATATCAACGCGAAAACCCGCCCCCGCTTGCATCACGTCCTGGCTTTTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATCCGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGAt                                                                                                                                                                                               <  2:368623/226‑1 (MQ=255)
                                                                cccGGTCCTTCAATAAAGCC‑AATATCACCGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGAAGAATGTAGTTACCGATGGTACTGCTGGCATAGATACGAATCGCGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGagcag                                                                                                                                                                                        <  1:104725/249‑1 (MQ=255)
                                                                   ggTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAgccg                                                                                                                                                                                     >  1:390392/1‑251 (MQ=255)
                                                                       tcccgatctaGCC‑AATACCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCAAAGTT‑CAACCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACGGCAGGCAGAATGTAGTTACCGATGTTACTACTGGCATAGATACAAATCGCGCCGTGGCCTTCGCAAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGccc                                                                                                                                                                                  <  1:428245/241‑1 (MQ=255)
                                                                           aaTAAAGCC‑AATATCACCGCGGAAACCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTC‑CAAGCGGCAACTGCGGATAATCATGGCGATAACGGGCAAAAACTGCAGGCAGAGTGTATTTACCGAGGGTACTACTGGCATAGATACGACTCGCGCCGTTTTCTTCGCAAAACAGTTGTGCGATTCAAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGtt                                                                                                                                                                             <  1:169511/251‑1 (MQ=255)
                                                                            aTAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGCGTTc                                                                                                                                                                            >  2:360083/1‑251 (MQ=255)
                                                                               aaGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCAAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCAGGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTCCTACTGGCATAGATACGAATCGCGCCGTGGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCAtt                                                                                                                                                                         <  1:400693/251‑1 (MQ=255)
                                                                                aGCC‑ACTATCCACGCGGACATCCAGCACCGCTTGCATCCCGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGccccgatcggacg                                                                                                                                                                        >  2:428245/1‑241 (MQ=255)
                                                                                   c‑AATATCACCGCGGAAACCCATCCCCGCTGGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTTCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCTATGGTACGACTGGCATAGAGACTAATCGCTCCGTTGCCTTCGGGAAACATTTGTTCGATGTCAACCGCCTGTTCAAGCAATGCGAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTaaca                                                                                                                                                                     <  1:477216/251‑1 (MQ=255)
                                                                                     aaattcacctcgGAAACCCAGCCCCTCTTGCACCACGTCCTGGCTATTCCCAACCCTAAGTT‑CAACCGGCAACGGCGTATAATCATGGCGAAAACGGGCGATACCGGCGGGCAAAATGTAGTTACCGATGTTACTTGGGGCATAGATCCGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTaacaa                                                                                                                                                                    <  1:390816/241‑1 (MQ=255)
                                                                                            ccgcggaacaccacccccGCTTGCATCACGTCCTGGCTATCCCCAACGCTAATTT‑CAAGCGGCAATGGCGGATAATCAGGGCGATACCGGGCGATAACTGCGGGCGAAATGTATTTCCCGATGGTACAACTGGAATAGAAACGAATCGCGCGGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCTGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCt                                                                                                                                                             <  2:38410/235‑1 (MQ=255)
                                                                                                    aTCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTccccc                                                                                                                                                     >  2:207844/1‑250 (MQ=255)
                                                                                                                 tGCATCACGTCCTGACTCTTCGCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGACGATAACTGCAGGCAGAATGTAGTTACAGCTGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAACCAGTTGTTCGATTTCCACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACACCCAGTCTTTTCCCCACGCGATCCAAc                                                                                                                                          >  2:195204/1‑249 (MQ=255)
                                                                                                                  ccaccACGCCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCAATAACGGGCGATAACTGCAGGCAGAATGTAGTTCCCGATGGTACTACGGGCATAGATACGAACCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGt                                                                                                                                       <  1:13990/247‑1 (MQ=255)
                                                                                                                       acgccgtGGCTATTCCCAACGC‑AAGTTTCAACGGGCACATCCGGAGAAACAGGGGGAGAACGGGCGATAACAGCAGAAATAAGGTATTTACCGGGGGTACTACTGGCATAAATCCGAACCCCGCCGTTGTCGTCGTGAATCATTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCAc                                                                                                                                  <  2:107551/245‑1 (MQ=255)
                                                                                                                              ggCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATACTCATGGCGATACCGGGCGATACCTGCAGGCAAAATGTAGTTACCGCTGATACTACTGGCCTAGATACGCATCGCGCCGTTGCCTTCGCGAAACCGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCCACGCACGCGGATAGACCCGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGc                                                                                                                           >  1:217061/1‑251 (MQ=255)
                                                                                                                                    tCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCt                                                                                                                     >  1:250409/1‑251 (MQ=255)
                                                                                                                                     cccAACGCTAAGTT‑CAATCGGCAACGGGGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGAATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCt                                                                                                                     <  1:133278/250‑1 (MQ=255)
                                                                                                                                      ccAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTc                                                                                                                   >  1:3960/1‑251 (MQ=255)
                                                                                                                                        cacgtcaaagcacAATCGGAAACGGCGAATAATCATGGCGATAACGGGCGATATCTGCAGGCGGAATGTATTTCCAGAGGGTCCTTCTGGCACAGATCCGACTCGCGCCGTTGTTTGCGCAAAACAGTTTTACGATTGCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTCCCCCACGCGAGCAAACAGTTGCACGCCAAGCTGCCCTTcc                                                                                                                  <  2:99989/239‑1 (MQ=255)
                                                                                                                                                     aaccGGCACCTGCGGAAAATCATGGCGATACCGGGCAATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGTATAAATACGAAGCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAgg                                                                                                     <  1:71653/248‑1 (MQ=255)
                                                                                                                                                                   gATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACACGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCAccgct                                                                                       >  1:253758/1‑251 (MQ=255)
                                                                                                                                                                            ggCGATAACGGGCGATACATGCAGGCAGAAGGTAGTTCCCGATGTTACTACTGGCATAGATACGAATCGCGCGGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGAc                                                                              <  1:343309/251‑1 (MQ=255)
                                                                                                                                                                                 tAACGGGCGCTAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGGTGTCTTAGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACCCACGCTGATAGAGCCGCCGCCCGAGTTCATTAACAACCAGTCTTTTCCCCCCGCAATCAAACAGTTGCACGCCACGCTGCCCTTCCAGGTCGGTCAAGTCTGCGCTCACTCCTGATTGCggcaccgc                                                                         >  2:442186/1‑244 (MQ=255)
                                                                                                                                                                                  aaCGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGCACTCCTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGAGCTCACTGCTGATTGCGACAACGcc                                                                        >  1:65993/1‑251 (MQ=255)
                                                                                                                                                                                    cGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGcc                                                                        >  2:134580/1‑249 (MQ=255)
                                                                                                                                                                                     gggCGATACCTGCAGGAAGCATGTAGATACCGATGGTACTACAGACATAGATACGAATAGCGCCGTTGTATTCGCGAACCAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCCACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCCAACAGTTGCACGCCAAGCTGCCCTTCCCGGCCGGTCCACGCTGCGCTCACTGCTGATTGCGACAACGCCAGc                                                                     >  2:302818/1‑251 (MQ=255)
                                                                                                                                                                                       gctaTAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGACGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGCTAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGGCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCAt                                                                   >  2:122976/4‑251 (MQ=255)
                                                                                                                                                                                        cGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATc                                                                  >  2:120445/1‑251 (MQ=255)
                                                                                                                                                                                         gATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGAGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCCGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCCCGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTAAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATc                                                                  >  2:416485/1‑250 (MQ=255)
                                                                                                                                                                                                gcaggaagAATGTGGTACCCGAGGGTCCTACAGGCATAAATCCGAACCGCCCCGTTGCCTTCGCAAAACATTTGTTCAATTCCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCGTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGAc                                                            <  2:240034/249‑1 (MQ=255)
                                                                                                                                                                                                  aggcagAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCt                                                        >  1:86837/1‑251 (MQ=255)
                                                                                                                                                                                                     cagAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTggg                                                     >  1:118325/1‑251 (MQ=255)
                                                                                                                                                                                                        aaTGTAGTTACCGTGGGTACTGCGTGCATAAATACGACGCGCCCCGTTGTCTTCGCAAAACATTTGTGCTGTTTCACCCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGggtgg                                                  <  2:170926/251‑1 (MQ=255)
                                                                                                                                                                                                         aggtttttcccGATGGTCCTCCTGGCATAATTACGAATCCCCCCGTTGTCGTCGCAAAACGTTTTTTCTATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGggtggt                                                 <  2:15815/242‑1 (MQ=255)
                                                                                                                                                                                                         aTGTAGTTACCGATGGTACTACTGGCATAGATACGAACCGCGCCGTTGTCTTCGCGAAACAGTTGTCCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGggtggt                                                 <  1:120445/251‑1 (MQ=255)
                                                                                                                                                                                                              gTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGAt                                             <  1:360083/250‑1 (MQ=255)
                                                                                                                                                                                                                        gTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCa                                   >  2:379365/1‑250 (MQ=255)
                                                                                                                                                                                                                          actactGGCATAGATACGAAGCGCGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAAt                                 >  1:103371/1‑250 (MQ=255)
                                                                                                                                                                                                                                ggcaaaaaTACGAATCGCCCCTTTGCCTTCGCGAAACAGTTTTTCGATTCCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACTCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTc                            <  1:71766/242‑1 (MQ=255)
                                                                                                                                                                                                                                  cATAGATACGAATCGCGCCGTTGCCTTCGCGACACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGc                         >  2:216266/1‑250 (MQ=255)
                                                                                                                                                                                                                                   aTAGATACGAATCGCGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCa                        >  1:94598/1‑250 (MQ=255)
                                                                                                                                                                                                                                   aTAGATACGAACCGCGCCGTTGTCTCCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCaa                       <  2:126777/251‑1 (MQ=255)
                                                                                                                                                                                                                                     aGATACGAATCGCGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCaaa                      >  1:167550/1‑250 (MQ=255)
                                                                                                                                                                                                                                            aaTCGCGCCGTTGCCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGCTAGAGAAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTcc               >  1:418124/1‑250 (MQ=255)
                                                                                                                                                                                                                                                        gTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTACCAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAggg  >  2:286873/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         
CGGCGCGGCGAAAACCACCAGCTCGTCTTCCAGCCACGGTTCAGAAATGATTTCAGTGCTGTGGCACGGTCCTTCAATAAAGCC‑AATATCAACGCGGAAATCCAGCACCGCTTGCATCACGTCCTGGCTATTCCCAACGCTAAGTT‑CAATCGGCAACTGCGGATAATCATGGCGATAACGGGCGATAACTGCAGGCAGAATGTAGTTACCGATGGTACTACTGGCATAGATACGAATCGCGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGG  >  NC_000913/2249112‑2249608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑