Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091399,2560TC38.6% 29.2 / 42.5 44V285A (GTA→GCA) ftsWpeptidoglycan glycosyltransferase FtsW
Reads supporting (aligned to +/- strand):  ref base T (16/11);  new base C (9/8);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTGTTGTTTGTGACTACGCTGGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCGGG  >  NC_000913/99009‑99496
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                
gtgtTGTTTGGGACTACGCTGGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACa                                                                                                                                                                                                                                               <  1:151414/251‑1 (MQ=255)
   ttgtttgtGACTACGCTGGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACaaa                                                                                                                                                                                                                                             >  1:424073/1‑250 (MQ=255)
         gtgACTACGCTGGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGGTTAGGTAACTCGGCACAAAAACTggg                                                                                                                                                                                                                                      >  1:235088/1‑250 (MQ=255)
                               gccgggCGGGGGCGAAATTGTGGATGTTCATTGCCATTATCGGTATGGGCAGTTCAGCGGTTGGTTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCacac                                                                                                                                                                                                                 <  2:382006/246‑1 (MQ=255)
                                   ggCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGGTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACt                                                                                                                                                                                                            >  1:276347/1‑251 (MQ=255)
                                     cGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACttt                                                                                                                                                                                                          >  1:284170/1‑251 (MQ=255)
                                           cGAAATTGTGGCAGTTCATTGCCATTCTCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGcc                                                                                                                                                                                                                             >  1:367482/1‑226 (MQ=255)
                                           cGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGcc                                                                                                                                                                                                                             <  2:367482/226‑1 (MQ=255)
                                            gAAATTGGGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTG‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCg                                                                                                                                                                                                   <  2:235088/251‑1 (MQ=255)
                                                  gtgGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGATGTGTC‑GCTGCTACTCGCAGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGACGATCCCTTTGGCCGCGGCTATCAGTTAACGCCATCGGTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAAATCGGCACAAAAACTGGCGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCAtt                                                                                                                                                                                              >  1:479594/1‑250 (MQ=255)
                                                    ggCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTTCCGGAAGCGCACACTGACTTTATTTTCGCCATTATc                                                                                                                                                                                           <  1:459447/251‑1 (MQ=255)
                                                     gCAGTTCATTGCCATTATCGGTATGGGCATTTCCGCGGTTGTGTT‑GCTGATACTCGCCGCACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACCAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCg                                                                                                                                                                                          >  2:27743/1‑251 (MQ=255)
                                                     gCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTT‑TGTTTGCTGATACTCGCCGACCCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGAGCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCGGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCg                                                                                                                                                                                          <  1:299922/251‑1 (MQ=255)
                                                        gTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCg                                                                                                                                                                                       >  1:336211/1‑251 (MQ=255)
                                                        gTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGCCGGAAGCGAACACTGACTTTATTTTCGCCATTATCGGCg                                                                                                                                                                                       >  2:77282/1‑251 (MQ=255)
                                                                     aTCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGt                                                                                                                                                                           <  1:414692/250‑1 (MQ=255)
                                                                            ggggCTTTTCGGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTAGCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGgt                                                                                                                                                                   <  2:284170/250‑1 (MQ=255)
                                                                                       gCGGTTGGGTT‑GCTGATACTCGCCGCACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCACGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACGCTGACTTTATTTTCACCATTATCGGCGAAGAACTGGGGGCTGTAGGTGTGGTGCTGGc                                                                                                                                                        >  2:403567/1‑251 (MQ=255)
                                                                                        cGGTTGTGTT‑GCTGATAATCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGACGATCCCTTTGGCAGCGGCTATCAGTTCCAGCAATCGCTGATGGCGTTTGGTCGCGGCGATCTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTCTGTCGGGGTGGTGCTGGc                                                                                                                                                        >  2:435928/1‑250 (MQ=255)
                                                                                               tgtagcggatattgcccgaccagtacggtaGCCGCCGTGTGACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTGAGGTAACTCGGCACAAAAACGGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTTTGCTGCTGGCACtttt                                                                                                                                                  <  2:334634/228‑1 (MQ=255)
                                                                                                           tCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTAttcttc                                                                                                                                     >  1:236902/1‑251 (MQ=255)
                                                                                                            cGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTAttcttc                                                                                                                                     <  1:364712/250‑1 (MQ=255)
                                                                                                             gcagAACCGTACCGTATCCGCCGTGTTAACGCCTTCTGGCACCCGTAAGAAGATCCCTATGGCAGCGGCTAACAGTTAACGCCATCGCTGATGGCGTTTGGTCGCGGCGAAATTTGGGGGCAAGGTTTAGGTAACTCGGCACCAAAACTGGAGTATATGCCGGCAGCGCACACTGACTTTATTTTCGCCATTATCGGCGACGAACTGGGGGATGTCGGTGGTGTGCTGGCCCCTTTAATGGAATTCAtcgt                                                                                                                                   >  2:408753/4‑251 (MQ=255)
                                                                                                               cGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCCTAGGACGATCCCTTTGGCCGCGGCTCTCAGTTAAACCAATCGCTGATGGAGTTTGGTCCCGGCGACCTTTGGGGCCAAGCTTTAGGTAACTCGGCACAAAACCTGGAGTCTCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGCGTGGTGCTGGCCCTTTTAATGGTATTCTtcgtcc                                                                                                                                 >  1:226871/1‑250 (MQ=255)
                                                                                                                gAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGc                                                                                                                                >  1:393606/1‑251 (MQ=255)
                                                                                                                gAACCGTACCGGATCCGCCGTGTTAACGCATTCTGGAACCCGTGGGAAGAGCCCTTTGGCAGCGGCTATCAGTTAACGAAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCGAGGTTTAGGTAACTCGGTACAAAAACTGGAGGATATGCCGGAAGCGAAAACTGACTTTATTTTCGCCATTATAGGCGAAGAACTGGGGCATATCGGTGTGGGGCTGGAACTTTGAATGGTAttctgtgacgc                                                                                                                                >  2:5821/1‑244 (MQ=255)
                                                                                                                 aaCCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCt                                                                                                                               >  1:334797/1‑251 (MQ=255)
                                                                                                                                  cgTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTTGCGCGATGTCGAt                                                                                                              >  2:376849/1‑251 (MQ=255)
                                                                                                                                  cgTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGGATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTTCTGGTACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGAt                                                                                                              <  1:322798/251‑1 (MQ=255)
                                                                                                                                   gtgtTGCCGCATTCTGGACCCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATGTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGAtt                                                                                                             <  2:269441/251‑1 (MQ=255)
                                                                                                                                        aCCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCGATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGcc                                                                                                         <  1:268291/250‑1 (MQ=255)
                                                                                                                                           gCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCCAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCCCTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTa                                                                                                      >  2:417837/1‑250 (MQ=255)
                                                                                                                                                              aaGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGGAACTCGGTACAAAAAATGGAGTATCTGCCGGAAGCGAACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGCTTGGCCGTACAGCATTAGAAATTGaccac                                                                                  >  2:418436/1‑251 (MQ=255)
                                                                                                                                                               aGATCACTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGaccacc                                                                                 >  1:372791/1‑251 (MQ=255)
                                                                                                                                                                               ggCTATCAGTTAACGCACTCGCTGCTGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGAAACTCGGTACAAAAACTGGAGTATCTGCCGGCAGCGCACACTGACTTTATTTTCGCCATTATCGGCGCAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGAATTCTTCGTCGCTTTTCGCGCGATGGCGCTTGGCCGTAAAGCATTAGCAATTGACCACCGTTTTTCCGGTTTtct                                                                 >  2:345224/1‑251 (MQ=255)
                                                                                                                                                                                   aTCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGcc                                                             <  1:193612/251‑1 (MQ=255)
                                                                                                                                                                                     cAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTGTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGTCACTTTTAATGGTATTCTTCGTCGCTTTTTGCGCGATGTCGATTGTCCGTAAAGCATTAGAAATTGACCTCCGTTTTTCCGGTTGTCTCGCCt                                                            <  2:287031/250‑1 (MQ=255)
                                                                                                                                                                                          aaCGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCtttt                                                                                                                            <  2:95353/181‑1 (MQ=255)
                                                                                                                                                                                          aaCGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCtttt                                                                                                                            >  1:95353/1‑181 (MQ=255)
                                                                                                                                                                                          aaCGCAATCGCTGATGGCGTTTGGACGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGttatt                                                      >  2:180575/1‑248 (MQ=255)
                                                                                                                                                                                              cAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGCACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTgg                                                  <  1:334012/251‑1 (MQ=255)
                                                                                                                                                                                                   gCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCt                                             <  2:424073/251‑1 (MQ=255)
                                                                                                                                                                                                                          aaCTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGtt                      >  2:82598/1‑251 (MQ=255)
                                                                                                                                                                                                                                              ggTAACTCGGCACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAATAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCTCCGTTTTTCCGGTATTCTCGCCTGTTCTATGGGGATCTGGTTTAGCTTCCAGGCGATGGGTAACGTAGGCGCGGCGGCggg  <  2:81027/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                
GTGTTGTTTGTGACTACGCTGGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGGCAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTT‑GCTGATACTCGCCGAACCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGCGGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGTTTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTCGCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTCTTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTTTCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTAGGCGCGGCGGCGGG  >  NC_000913/99009‑99496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑