| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,381,720 | 0 | T | A | 26.6% | 133.0 / 17.7 | 64 | Y200F (TAT→TTT) | frdA | fumarate reductase flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base T (43/4); new base A (0/17); total (43/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.34e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTCGATTTTCATGC > NC_000913/4381478‑4381963 | tGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAAcgcg > 1:260775/1‑250 (MQ=255) ggCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCAcc > 1:262941/1‑251 (MQ=255) ggCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGAGATACTGAAC‑GAATTCCATGTCCCGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGGGTTGTAACGATCAACGCGACCCGCAcc > 2:369670/1‑251 (MQ=255) cctgCCCAGCGGAGTTCCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCg < 2:397180/247‑1 (MQ=255) tCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATCACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTAACCGCGTCAACCTTCGGTCATCAGG‑ATACCGGACCCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGcc > 2:319069/1‑251 (MQ=255) gCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCg > 1:397180/1‑247 (MQ=255) ggAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCAt > 2:89058/1‑251 (MQ=255) aGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATa > 1:112571/1‑250 (MQ=255) ccattGCCGTAATCTTGCAGATACCGGTAGCCTTTTTTGTTGACCAGAATCCCGCCTTCCCCGCGGCACCCTTCGGTCACCAGG‑ATCCCGAACCCTGG‑AAGCCCGGTTGGGTGATCCTGACC‑GAATCCCATGTCACGCAGCGGAACGCCG‑GGG‑CTTAGCGCCATCCCCATCCCGCCCCCGGAAACGATGCC‑GCGGT‑TGGTGTTGAAACGAAAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGtt < 1:57941/247‑1 (MQ=255) ccATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGtt > 1:245512/1‑250 (MQ=255) aTGCCGTAATCTTGCAGATACCGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑CTACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGc > 2:476382/1‑251 (MQ=255) gaaaTCTTGCAGAAACCGGTAGCCTTTTTTTTTTACAAGAATACCGCCTCCCCCGGGGCACCTTTCGTTCAGCAGG‑ATACCG‑AACCTGGGCACACCGTTTGGGTAATAC‑GAACCGAATTCAATGTCCCGCAGCGGAACGCCG‑TGG‑CTTATCCCCATCCCCATACCGTCCCCGGAAACGTTGCC‑GCTGT‑TGGTGTTTTAACGAAAAACGCGCCCGGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGa < 1:247484/249‑1 (MQ=255) ttGCAGAAAGCGGTGGCAATTTTTGTTGACCAAAATCCCCCCTCCACCGCGGCACCCTCCGGTCATAAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATTTCACGCAGCGGACCGCGG‑TGG‑CTTAGCGCCATCCCCATCCCGCCACCGGAAACGATGCC‑GCGGT‑TGGTGTTGTAACGATAACCGCGCCCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGc < 1:346428/250‑1 (MQ=255) gCAGATAACGGTAGCCTTTTTTGTTGACAAGAATCCCGCCTCCCCCGCGGCACCCTGCGGTCATCAGG‑ATACCGAAACCGGG‑CAGACCGGTGGGGTGATACTGAAC‑AAATCCAATGTCACGCAGCGGACCGCGG‑TGG‑TTTAGCGCCATCCCCATCCCGTCCCCGGAAACGATGCC‑GCGGT‑TGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCAc < 1:225737/250‑1 (MQ=255) aGATAACGGTAGCCATTTTTGGTGACCAGAATACCGCCTTCACCGCGGCAAACTTCGGTCATCAGG‑ATAACGGAACCAGG‑CAAACCGGTTGGGTGCTACTGCAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCCTAACGACCGCGTTAGCACGGATCTGCAcc > 2:428635/1‑249 (MQ=255) tAACGGTAGCCATTTTTGTTGACCAGAATCCCGCCTTAAACCAGGCCCCCTTCGGTCATCAGG‑CTACCGGCCCCTAG‑CAAACCGGTTGGGTGATACTGCCC‑GAATTCCATGTCACGCAGCGGCACGCCG‑TGG‑CTTAGCGCCATCCCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCgg > 1:200563/1‑250 (MQ=255) aCGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGc > 1:83543/1‑251 (MQ=255) ttgttgACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCCCGGATCTGCACCAGCGTGCCTTCCATCATGtt > 2:367072/1‑251 (MQ=255) ttgcccAGAATCCCCCTTTCCCCGCGGCA‑CCCTCGGTTCTCAGGGATACGGGACCCTGG‑CGGACCGGTGGGGTGATACTGACC‑GAATTCCATGTCACGCGGCGGACCGCGG‑TGG‑TTTAGCCCCATCCCCATCCCGTCCCCGGAAACG‑TGCCCGCCGT‑TGGTGTTGTAACGAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCa < 1:222576/246‑1 (MQ=255) gACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTg > 1:180076/1‑251 (MQ=255) aGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGCACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTAc > 1:295020/1‑251 (MQ=255) aatcccGCCTGCCCCGCGGCAACGTCCGGTCATCAGG‑AGTCTGAACCCTGG‑GAGGCGGTTTGGGTGATACTGAAG‑GAATTCCGTGTCACGCGGCGAAACGCGG‑TGG‑GTTAGCGCCATACCCTTCCCGCCGCCGAAAAGAATGCC‑GCCGT‑TGGTTGTGTAACAAAAAGCGGCCCCCGCTCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCa < 1:480926/247‑1 (MQ=255) aTACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGCAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTAcc > 2:50409/1‑249 (MQ=255) ccGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGcc > 2:203067/1‑251 (MQ=255) ttCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGa > 1:252950/1‑250 (MQ=255) cgcgGCAAACTTCGGTCATCAGG‑ATACCCGAACCTGG‑CACACCGGTCGGGGGATACTGCAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TAG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GACGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACCACCGCGTTAGAACGGATCTGCACCAGCGTGCCTTCCACCATGTTCACTGCTACCAGGCCGCGAACA‑Tg > 2:57370/1‑250 (MQ=255) ggCAACCTTCGCTCATCCGG‑ATACCGGAAACGGG‑CAGCCGGGTTGGGTGCTAATGCAC‑GAATTCCATGACACGCAGCCGAACGACG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCACCAGTAGCCATCACGACCGCGTTAGCACGGATCTGAACCAGCGTGCCTTCCATCATGATCATTGCTACCAGGCCGCGCACA‑TGAcc > 2:249894/1‑250 (MQ=255) aaCCTTCGGTCATCAGG‑ATCCCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACcatc > 1:174576/1‑250 (MQ=255) aaCCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGCACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCAAGGCTCTGCACCAGCGTGCCTTCAATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACcatcc > 2:170035/1‑250 (MQ=255) cccTTGGGTCACCAGG‑ATCCCGAACCCGGG‑CAGCCCGGTGGGGTGATACTAACC‑AAATCCCAGTTCACGCAG‑GGAACCCCGGTGG‑CTTAGCGCCATCCCCACCCCTTCCCCGGAAACGATCCC‑GCCTT‑GGGTGTTTTAACAAAAACCGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACcatcat < 1:37973/250‑1 (MQ=255) ccTTCGGTCAACAGG‑ATCCCGGACCCTGG‑CCGCCGGGTTGGGTGATACTAAAC‑GAATTCCATGTCCCGCGGCGGAACGCCG‑TGG‑CTTAGCGCCATCCCCATCCCGCCCCCGGTACCGATGCC‑GCCGT‑TGGTTTTGTAACGATAACCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCatcatc < 1:109531/251‑1 (MQ=255) gTCACCAGG‑ATCCGGGACCCGGG‑CAGCCCGGGTGGGTGATACTGAAC‑GAATTCCATGTCCCGCGGCGAAACGCGG‑GGG‑CTTAGCGCCATCCCCATCCCGCCCCGGGAAACGATGCC‑GCCGT‑TGGTGTTGTAACGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAg < 1:161348/251‑1 (MQ=255) caGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACACATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCCTCATGTTAATTGCTACCAGGCCGCGA‑CACTGACCCTCATCCACCAGAatat > 2:186891/1‑251 (MQ=255) gg‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATcc > 1:355206/1‑251 (MQ=255) ccccGGACCCGGG‑CAGCCCGGTGGGGTGATTCTGAAC‑GATTCCCATGCCACGCCGGGGAACGCC‑‑TGGTCTTAGCCCCATCCCCATACCGCCCCCGGAAACGATCCC‑GCCGT‑TGGTTTTGTAACAAAAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGc < 1:209189/249‑1 (MQ=255) cgggacccgggcagcccgtgtGGGGGATACTGAAC‑GAATCCCATGCCCCCCAGCGGACCGCGG‑TGG‑CTAAGCCCCCCCCCCACACCGCCCCCGGAAACGGTCCC‑GCGGT‑TGGTGTTAAAACGAAAAACGCGCCCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCAc < 2:83543/231‑1 (MQ=255) aaCCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGaa > 2:88012/1‑250 (MQ=255) ccTGG‑CAGACCGGTTCGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCCTAACGACCGCGTTAGCACGGATCTGCACCAGCTTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCACCCAGAATATCCAGCACGAAATg > 2:234423/1‑251 (MQ=255) tGG‑CAGACCGGTTGGGTGATAATGAAC‑GAATTCCATGTCACGCAGCGGAACGACG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTCACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGtt > 2:396414/1‑251 (MQ=255) gg‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGCACGCCG‑TGG‑CTTAGCGACATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGCAGCCATAACGACCGCGTTCGCACGGATCTGCACCAGCGTGCCTTCCATCATGCTCATTGCTCCCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATACAGCACGCAATGTTc > 2:186511/1‑251 (MQ=255) gACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGACG‑TGG‑CTTAGCGCCATAACCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTc > 1:329901/1‑250 (MQ=255) aCCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCa > 1:366621/1‑250 (MQ=255) aaTTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTACCGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATAATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCCACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTg > 2:284833/1‑251 (MQ=255) aTTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGAcc > 1:348806/1‑190 (MQ=255) tCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATAACGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATCGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGACTTGc > 2:274963/1‑249 (MQ=255) gaggCGGAACGCCG‑TGG‑CTTACCGCCAACCCCACACCAGCCCCGGTAACGGTGCC‑GCCGTGTGGT‑TTGTAACAATAAACGCGACCCCCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAAGGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTgg < 1:166345/248‑1 (MQ=255) ggaaaCGCCG‑GGT‑CTGAGCGCCACCCCCATCCCGTCCCGGGTAACGATGCC‑GCGTT‑TGGTGTTGTAACGAAAAACGCGCCCCGCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACa < 1:89058/248‑1 (MQ=255) cGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAACCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCAGGAAATGTTCGTCAACACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCgtg > 2:275790/1‑251 (MQ=255) gctaacccccatacccatacCGCCACCGTAAACGAGGCC‑GCTGT‑TGGTTTTGTAGCGAAAACCGCGCCCCGCTCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTgcagca < 1:50409/243‑1 (MQ=255) catacccataccGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGc > 2:319733/1‑251 (MQ=255) cccataccGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCggg > 1:436732/1‑250 (MQ=255) atcccgccccgggTAACGATGCC‑GCGGT‑TGGTGTTGTAACGAAAAACGCGCCCCGCCCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCtt < 1:476382/240‑1 (MQ=255) cctccccGGTAACGATGCC‑GCCGT‑TGGGTTTGTAACGAAAACCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATcg < 1:396414/246‑1 (MQ=255) gTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAAAGCGACTAGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGTTCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATcgg > 1:42064/1‑251 (MQ=255) tCACCGGTAACGATGCC‑GCGGT‑TGGTTTTGTAACAAAAACCGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATcg < 1:129586/249‑1 (MQ=255) ccccgggTAACGTTGCC‑GCCGT‑TGGGGTTTAAAAGAAAAACGCGCCCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATcggt < 1:236947/246‑2 (MQ=255) aaCGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCACAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAAcc > 1:186994/1‑251 (MQ=255) aaCGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAAcc > 1:259044/1‑251 (MQ=255) aagttcgcgggctggggttttatAACGAAAAACGCGACCCGCGCCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCGGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGTGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAAcc < 1:41432/229‑1 (MQ=255) gcc‑gccGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGACCCAggggc > 2:189543/1‑248 (MQ=255) gT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATTACGCCCGCGTTAGCAGGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCAGGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGAACGCTCg > 1:275320/1‑250 (MQ=255) gT‑TGGTGTTGTAACGATAAACGCGACCAGCACCGCCAGTAGCCATAACGAACGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCCACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGAGTGCAGCATATGGAAGCCGGCCTTATCGGCGGCGAACCAGGGGCGCTCgg > 2:52588/1‑250 (MQ=255) tgtAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTCGATTTTCATg > 1:397956/1‑251 (MQ=255) gaaaCGAAAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTCGATTTTCATgc < 1:189543/249‑1 (MQ=255) | TGTTTTTCGGCTCGCCCAGCGGAGTTTCCGGGCCCATGCCGTAATCTTGCAGATAACGGTAGCCATTTTTGTTGACCAGAATACCGCCTTCACCGCGGCAACCTTCGGTCATCAGG‑ATACCGGAACCTGG‑CAGACCGGTTGGGTGATACTGAAC‑GAATTCCATGTCACGCAGCGGAACGCCG‑TGG‑CTTAGCGCCATACCCATACCGTCACCGGTAACGATGCC‑GCCGT‑TGGTGTTGTAACGATAAACGCGACCCGCACCGCCAGTAGCCATAACGACCGCGTTAGCACGGATCTGCACCAGCGTGCCTTCCATCATGTTCATTGCTACCAGGCCGCGAACA‑TGACCATCATCAACCAGAATATCCAGCACGAAATGTTCGTCAAAACGCTGGATCTGCGGGAATTGCAGAGAGGTCTGGAACAGCGTGTGCAGCATATGGAAGCCGGTCTTATCGGCGGCGAACCAGGTGCGCTCGATTTTCATGC > NC_000913/4381478‑4381963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |