Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,379,638	0	G	A	28.6%	76.2 / 10.2	43	F45F (TTC→TTT)	frdC	fumarate reductase membrane protein FrdC
Reads supporting (aligned to +/- strand):  ref base G (26/4);  new base A (0/12);  total (26/17)
Fisher's exact test for biased strand distribution p-value = 1.65e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCGCCCAGAGACTTTTGATAATTGGCTCTGGTCCCATTTTTTCGTCTTTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGC  >  NC_000913/4379388‑4379886                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                 gggCAACAAACAGAATTACGATGGTGGCATCCACAGTTACCGCCCAGAGACTTTTGATAATTGGCTCTGTTCCCATTTTTTCGTCTGTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑GTTTGT‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGAGCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGG‑TT‑CCGTG‑CCATTTGTC‑A‑TGT‑CAAACAGCCCg                                                                                                                                                                                                                                                                  <  2:288908/251‑1 (MQ=255)        aaacaGGATTACGATGGTGGCAACCACAGTTACCGCCCAGAGACTTTTGATAATTGGCTCTGGTCCCATTTTTTCGTCTTTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATc                                                                                                                                                                                                                                                            >  1:177588/1‑250 (MQ=255)                ttACGATGGCAGCAACTACAGTTACCGCCCCGAGACTTTTAATAATTGGCTCTGATCCCATTTTTTCGTCTTTTACACTGATATTGGCCGCTTTCGGTGCCCGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGAAAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCCTTTTTC‑A‑GGG‑CCAACCGCCCGAAAATAAGTT‑CAA‑Tg                                                                                                                                                                                                                                                 >  2:212188/1‑251 (MQ=255)                 tACGATGGTGGCAACCACAGTTACCGCCCAGAGACTTTTGATAATTGGCTCTGGTCCCATTTTTTCGTCTTTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑Tg                                                                                                                                                                                                                                                 <  1:324160/250‑1 (MQ=255)                    gATGGTGGCAACCACAATTACCGCCCAAAGACTTTTGATAATTGGCTCTGGACCCATTTTTTCGTCTTTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCCTTTTTC‑A‑GGG‑CAAACAGCACGACAATCAGTT‑CAA‑TGCTg                                                                                                                                                                                                                                              >  2:44686/1‑250 (MQ=255)                          ggCAACCACAGTTACCGCCCAGAGACTTTTGATAATTGGCTCTGGTCCCATTTTTTCGTCTTTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACcac                                                                                                                                                                                                                                       <  1:224976/250‑1 (MQ=255)                                       aCCGCCCAGAGACTTTTGATAATTGGCTCTGGTCCCATTTTTTCGTCTTTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAAcc‑gc                                                                                                                                                                                                                    >  2:137240/1‑251 (MQ=255)                                       aCCGCCCAGAGACTTTTGATAATTGGCTCTGGTCCCATTTTTTCGGCTGTTACAAGTATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTGTGTAAAAATTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAATCAGCCTGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAAcc‑gc                                                                                                                                                                                                                    <  1:428175/251‑1 (MQ=255)                                          gCCCAGAGACTTTTGATAATTGGCTCTGGTCCCATTTTTTCGTCTTTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGt                                                                                                                                                                                                                 >  2:410238/1‑251 (MQ=255)                                                        gATAATTGGCTCTGGTGCCATTTTTTTGTCTTTTACAATGATATTGGCAGCTTTCGGTGAAAGCTTAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCTCCAGATCGATCAGGTTAATGATCACGATAACCGGGTTTTGCAAAAAGTCGAAGAATCCCGCACAGGC‑TT‑CCGTC‑CCATTTTTA‑A‑GGG‑CAAACAGCACGAAAATAATTT‑CGA‑TGCTGA‑ACCAC‑AC‑A‑T‑ACGCAAcc‑gccgcgacctttcccctct                                                                                                                                                                                                   >  2:260515/1‑236 (MQ=255)                                                                           aTTTTTTCGTCTTTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACgg                                                                                                                                                                                >  2:181347/1‑251 (MQ=255)                                                                              ttttCGTCTTTTACACAGATATTTGCCGCCTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGATGCCGCCAGAGTGATCAGGTTAATGATCACGATCACCGGGTTTTGTAACAAGTCGACGAATACCGCCCAGGC‑TA‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGATAATCAGTT‑AAA‑TGCTGA‑ACCAC‑AC‑C‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAACCGGCa                                                                                                                                                                              >  2:292992/1‑250 (MQ=255)                                                                                                     gccggCTTCCGGTGCCAGTT‑AAACCCGG‑TTTTGG‑GGGGCAAGATGGGCTGCCGCAAGAGTGATCAGTTAAATGATCCCGATAGCCGGTTTTTCAAAAAAGTCGACGAACCCCGCCC‑GTC‑TTTCGGGG‑CCTTTTTTC‑A‑GGG‑CAAACAGCCCAAAAAGCAGTA‑AAA‑GGCTAA‑CCCCC‑CC‑A‑C‑CGGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCa                                                                                                                                                      <  1:285009/247‑1 (MQ=255)                                                                                                               ggTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTAc                                                                                                                                            >  2:28761/1‑251 (MQ=255)                                                                                                                        tCAAACCAGGGTTTTGG‑TGGGCA‑GAGGGGCTGCCGCAAGAGTGAGAAGTTAAATGATCACAATACCGGGGTTTTAAAAAAAGCCGAC‑AACCCCCCCCCGGCCTC‑CCGGG‑CATTTTTTC‑G‑GGC‑CAAACGGCCCAAAAATAAGTT‑AAA‑GGCTAA‑CCCCC‑AC‑A‑G‑CCGAAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGGCGTCATTGGCCGTACATACGGt                                                                                                                                     <  1:137240/250‑1 (MQ=255)                                                                                                                           aaCCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACg                                                                                                                                >  2:62269/1‑251 (MQ=255)                                                                                                                             cccgg‑TTTTGGGTGTGAA‑GAAGAGCTGCCGCCAGAGTGACCAGTTAAAGGACCACAAAACCCGGTTTTTGAAAAAAGCCGCCAAACCCCCCCCAGCC‑TT‑CCGG‑‑CCATTTTTCCA‑GGG‑AAAACAGCCCAAAAATCAGTT‑CAA‑TCCTGA‑CCCAC‑AC‑A‑G‑CCGAAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGt                                                                                                                               <  1:148231/248‑1 (MQ=255)                                                                                                                               aGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCAACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGttt                                                                                                                             >  2:130245/1‑250 (MQ=255)                                                                                                                                   ttttGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAACCAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAgtc                                                                                                                         >  2:169763/1‑251 (MQ=255)                                                                                                                                      tGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAgtcgtc                                                                                                                      >  1:371607/1‑251 (MQ=255)                                                                                                                                      tGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAgtcgtc                                                                                                                      >  1:307432/1‑251 (MQ=255)                                                                                                                                       gg‑TGTGCA‑GCAGAGCTGAAGCCAGAGTGATCAGGCTAATGATAGCGATAACCGGGTTTTGTAAAAAGTCGAGGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGACAATCAGTT‑CAA‑TGCTGA‑ACTAC‑AC‑A‑G‑CCTGAACC‑GCCGTGCATTCGCGCAGCCTGTAAAAGCGATAAAACGGCACTTGTTTCCACCAGTTGTACGTGATTGGCCGTACATACGGTTTACGTTTAgtcgtc                                                                                                                      >  2:76207/1‑250 (MQ=255)                                                                                                                                        gggtgtcaagaagggctcccgccAGAGTGATAAGGTAAATGACCACAATACCGGGGTTTTGAAAAAAGCCGACGAACCCCGCCCAGGC‑TG‑CCGGG‑CC‑TTTTTC‑A‑GGGGAAAACACCCCAAAAATAATTT‑CAA‑T‑CCGA‑ACCCCCAC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAgtcgt                                                                                                                       <  1:169763/232‑1 (MQ=255)                                                                                                                                                   agagCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGACCC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTc                                                                                                           >  2:435235/1‑251 (MQ=255)                                                                                                                                                        tGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCtt                                                                                                        >  2:334406/1‑249 (MQ=255)                                                                                                                                                                 agTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTc                                                                                             >  1:166243/1‑251 (MQ=255)                                                                                                                                                                     aTCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAggg                                                                                         >  2:11710/1‑251 (MQ=255)                                                                                                                                                                       aaGGTTAATGATCACGATAACGGGTTTTTGAAAAAATGCGACGAACCCCCCCCAGCC‑TC‑CCGGC‑CCTTTTTTC‑A‑GGC‑CAAACAGCCCAAAAAGCAGTA‑CAA‑TGCTGA‑ACCCC‑AC‑A‑G‑CGGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTc                                                                                       <  1:435235/250‑1 (MQ=255)                                                                                                                                                                              aTGACCACGATAACGGGGTTTTGAAAAAAGTCGACGAACCCCGCCCAGGC‑TT‑CCGGC‑CAATTTTCC‑A‑GGG‑CAAACAGCCAAAAAACCATTT‑AAA‑TGCGGA‑ACCAC‑AC‑G‑G‑CCGAAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATaa                                                                                <  1:181347/251‑1 (MQ=255)                                                                                                                                                                               tGATCACGCTAACCCGGTTTTGTAAAAAGTCGCCGACTCCCGCCAAGCC‑TT‑CAGGG‑CCATTTTTC‑A‑GGG‑CAAACCGCCCGAACCTCCGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTCAAAGCGATAAAACGGCAATTTTTTCCACCAGGAGGACGTCATTGGCCGTCCATACGGTTTAAGTTTAGTCGTCATGTTGTACTCCTTAGCGTGGTTTCAGGGTCGCGATa                                                                                 >  2:182416/1‑249 (MQ=255)                                                                                                                                                                                 aTCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGaa                                                                                                                                                                                                                         <  2:343620/112‑1 (MQ=255)                                                                                                                                                                                         aaCCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTc                                                                     >  2:179365/1‑251 (MQ=255)                                                                                                                                                                                               ttgttaaaaaagcccgacgaACCCCGCCCAGG‑‑TT‑CCGGGACCATTTCCC‑G‑GGG‑CAACCAGCCCAAAAATAAATT‑CAA‑TG‑TGA‑ACCAC‑CC‑CCG‑CCGAAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACt                                                                <  2:112167/236‑1 (MQ=255)                                                                                                                                                                                                 attgaaaaaaGGCGACTACTCCCGCCCGGGT‑GT‑CCGGG‑CCTTTTTTC‑A‑GGC‑CAACGAGCCAAAAAATAATTT‑AAA‑TGCTGA‑‑CCCC‑AA‑A‑GACCGGAACC‑GTCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAAGTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACttt                                                              <  1:228086/245‑1 (MQ=255)                                                                                                                                                                                                   aaaaaaaaGTCGACAACCCCCGCCCAGCC‑TC‑CCGGG‑CTGTTTTAC‑A‑GGC‑CAAACACCCCAAAAATCCTTA‑CAA‑TGATAA‑CCCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCTATAAGAAAGTCTTTCGAACTTTCt                                                            <  2:305956/247‑1 (MQ=255)                                                                                                                                                                                                     aaaaaagccaccaaaccccGCCCAGGC‑TT‑CCGG‑‑CCATTTTTCCA‑GGG‑AAACCACCCCAAAAACCA‑TT‑CAAAGGCTAA‑ACCAC‑AC‑A‑G‑CCGAAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACt                                                         <  1:28761/235‑1 (MQ=255)                                                                                                                                                                                                               acgaATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCctgct                                               >  1:216785/1‑251 (MQ=255)                                                                                                                                                                                                                ccgacccccgccAGGC‑TG‑CCGGG‑CCATTTTTA‑C‑GGG‑CAAACCCCCCAAAAAAGAGTTACAA‑TGC‑GG‑ACCAC‑ACCG‑G‑CGGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCctg                                                 <  2:236253/239‑1 (MQ=255)                                                                                                                                                                                                                  aaTCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGa                                                                   >  1:16742/1‑228 (MQ=255)                                                                                                                                                                                                                  aaTCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGa                                             >  1:375806/1‑250 (MQ=255)                                                                                                                                                                                                                     cccccccccgggct‑CCGGG‑CCTTTTTTC‑AAGGC‑CAAACGCCCCAAAAATCAGTT‑AAA‑GGTTAA‑CCCAC‑AC‑A‑G‑CGGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAAt                                           <  1:11710/238‑1 (MQ=255)                                                                                                                                                                                                                          ccAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAACTCAGTT‑CAA‑TGCTGA‑CCCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACt                                                                >  2:469832/1‑223 (MQ=255)                                                                                                                                                                                                                                     cGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCg                           >  2:261704/1‑251 (MQ=255)                                                                                                                                                                                                                                              atttgagcagg‑CAAACACCCCCAAAAACAGTT‑CAA‑AGGCGACACCAC‑AC‑‑‑G‑CCGGAACCCGCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTGCATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACgtg                      <  2:255334/242‑1 (MQ=255)                                                                                                                                                                                                                                                                    gAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGc  >  2:9186/1‑249 (MQ=255)                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                 GGGCAACAAACAGGATTACGATGGTGGCAACCACAGTTACCGCCCAGAGACTTTTGATAATTGGCTCTGGTCCCATTTTTTCGTCTTTTACAATGATATTGGCCGCTTTCGGTGCCAGTTCAAACCAGG‑TTTTGG‑TGTGCA‑GCAGAGCTGCCGCCAGAGTGATCAGGTTAATGATCACGATAACCGGGTTTTGTAAAAAGTCGACGAATCCCGCCCAGGC‑TT‑CCGGG‑CCATTTTTC‑A‑GGG‑CAAACAGCCCGAAAATCAGTT‑CAA‑TGCTGA‑ACCAC‑AC‑A‑G‑CCGGAACC‑GCCGTGCCTTCGCGCAGCATGTAAAAGCGATAAAACGGCAATTTTTTCCACCAGGTGGACGTCATTGGCCGTACATACGGTTTACGTTTAGTCGTCATGTTGCACTCCTTAGCGTGGTTTCAGGGTCGCGATAAGAAAGTCTTTCGAACTTTCTACTTTGCCCTGCTGAATGGCCGCAGCCGGATCGACGTGTTTCGGGCAGACTTCGGAGC  >  NC_000913/4379388‑4379886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑