| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913 | 2,449,820 | (GGA)3→2 | coding (228‑230/822 nt) | yfcO ← | DUF2544 domain‑containing protein YfcO |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 2449816 = | 3 (0.050) | 36 (0.660) | 34/474 | 0.4 | 94.8% | coding (234/822 nt) | yfcO | DUF2544 domain‑containing protein YfcO |
| ? | NC_000913 | = 2449812 | 1 (0.020) | coding (238/822 nt) | yfcO | DUF2544 domain‑containing protein YfcO | |||||
AAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTTTGTTGATATTTTGATGGTAGCCAATCAGCTTAACCGAAGGGGCAGGAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCAATAAGCATGACCAGAGCGAACAACCATCGT > NC_000913/2449569‑2450038 | aaGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGCTACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATAACCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTTTGTTGATATTTTTATGGTAGCCAATCAGCTTAACCGAAGGGGCaggaggg > 2:292602/1‑250 (MQ=255)aaGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCGTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTTTGTTGATATTTTGATGGTAGCCAATCAGCTTATCCGAATGGGCaggagg < 2:54278/250‑1 (MQ=255) tgaacagaaacggatggacaaatcgtgtcgtgactatcaggcccctgatacgtcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgtggatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCAc > 1:188865‑M1/240‑250 (MQ=255) gaacagaaacggatggacaaatcgtgtcgtgactatcaggcccctgatacgtcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtagaatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTc > 1:154848‑M1/239‑251 (MQ=255) gaaacggatggacaaatcgtgtcgtgactatcaggcccctgatacgtcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAggaggaccaatacagcc < 2:58079‑M1/18‑9 (MQ=255) ccGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGCGAGGCTTCAGCGTCATTCATCCTCCCCAACCCTGGCCACGCAACGTCAGCTTCACTCTCTGCAGCGCCACTAACCTCCCCTGTCTAATACCCGCCGTTCCACCCAGACGGTAATCCAGGCCCCCTTGCTTTGTTGCTATTTTGATGGTCGCCCCTCAGCTTAACCGACGGGGC‑‑‑AGGAGGACCACTCCAGCTcac > 1:151080/2‑251 (MQ=255) aaacggatggacaaatcgtgtcgtgactatcaggcccctgatacgtcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatagtagccaatcagcttaaccgaaggggcAGGAGGACCACACCAGCTc > 2:181250‑M1/233‑251 (MQ=255) acggatggacaaatcgtgtcgtgactatcaggcccctgatacgtcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTcac > 2:338375‑M1/231‑251 (MQ=255) acaaatcgtgtcgtgactatcaggcccctgatacgtcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGc < 1:69741‑M1/28‑1 (MQ=255) aatcgtgtcgtgactatcaggcccctgatacgtcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAat < 1:269950‑M1/31‑1 (MQ=255) atcgtgtcgtgactatcaggcccctgatacgtcacgccccatggagaggcttcagagtcattcataataaccaaacatggacaagcaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAatat < 1:208996‑M1/33‑1 (MQ=255) atcaggcccctgatacgtcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATg < 2:105545‑M1/46‑1 (MQ=255) caggcccctgatacggcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGc > 2:263275‑M1/203‑251 (MQ=255) ggcccctgatacgtcacgccccctggagaggcttcagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGcc < 1:319199‑M1/50‑1 (MQ=255) gtcacgccccctggagaggcttcagagtcattcataataaccaaccatggaccagacaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatcccggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACtttt < 2:301817‑M1/63‑1 (MQ=255) cagagtcattcataataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGc > 1:292602‑M1/167‑250 (MQ=255) ataaccaaccatggacaagaaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACGTTTATATTCACCAACGTACACTGCGGATGGCATCattat > 2:319199‑M1/153‑251 (MQ=255) aaaagtaagattcaatctctgcagagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGc < 1:130615‑M1/117‑1 (MQ=255) agagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTAttttt < 1:143811‑M1/103‑1 (MQ=255) agagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAAGATTACGCGAATCACCGTTGt > 2:284884‑M1/112‑251 (MQ=255) agagacaataacctcaaatgtataatacccgcagttccacccagacggtaatccaggacaacttgctttggtgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTAttttt > 2:143811‑M1/112‑214 (MQ=255) gtataatacccgcagtcccactcagacgtgaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATAGTTCCCCATTCATGAGCCGTTGACTACTGTTATATGCACCAACGGACACTTCGGATGGCATCATGATTTTTAAAGTAAAAGTCGCGATATTACGCGAATCTCCGTTGTAACCGTAGTAAACGTCAAc > 1:444197‑M1/93‑251 (MQ=255) acccgcagtaccacccagacgggaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTGCCCCATTCATGAGCCATTGACGACTGTGATATTCACCAACGTACACTGCGGATGGAAGCATTATTTGTAAATTAAAAGTCGCAATATTACACGAATCACCGTTGTACCCTTAGTAAACGTCAACGCTATGc > 2:239688‑M1/86‑251 (MQ=255) ccacccagacggtaatccaggacaacttgctttgttgatattttgatggtcgccaatcagcttaaccgaaggggcAGGCGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGcc < 2:455259‑M1/175‑1 (MQ=255) acccagacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCAt < 1:97362‑M1/177‑1 (MQ=255) gacggtaatccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTc < 1:62122‑M1/183‑1 (MQ=255) ggtaatccaggacaacttgcgttgttgatattttgatgggagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTGATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTgt > 2:103453‑M1/66‑251 (MQ=255) atccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTgtagta > 1:244091‑M1/62‑251 (MQ=255) ccaggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGtagtag > 2:97362‑M1/60‑250 (MQ=255) aggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCaa > 2:62122‑M1/58‑251 (MQ=255) aggacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggaAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCa < 2:298998‑M1/193‑1 (MQ=255) gacaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCAAATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCAAt < 1:304800‑M1/195‑1 (MQ=255) acaacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTCCGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCAATa < 2:389562‑M1/196‑1 (MQ=255) caacttgctttgttgatattttgatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCAATAAg > 1:105545‑M1/54‑251 (MQ=255) gatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCAATAAGCATGACCAGAGCGAACAACCAt > 1:301817‑M1/32‑251 (MQ=255) gatggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCGATGCCCGGCAGCCATAGCTTCTGTAGTAGCAATAAGCATGACCAGAGCGAACAACCAt > 2:98536‑M1/32‑251 (MQ=255) atgatagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAAGCACCGTTGTAACCGGAGGAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCAATAAGCATGACCAGAGCGAACAACCata > 2:151080‑M1/31‑250 (MQ=255) tggtagccaatcagcttaaccgaaggggcAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCAATAAGCATGACCAGAGCGAACAACCATCg < 1:408685‑M1/222‑1 (MQ=255) ggtagccaatcagcttgaccgaaggggcAGGAGGACCACTCGAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTTACTGCTTTTATATTCACCAACGTACACTGCGGATGGCAGCATGATTTATAAATTAAAAGTCGCAATGTTACGCGAATCCCCGTTGTAACCGTTGTAAACGTCAACGCTATGCCCGGGATTCATAGCGTCTGTAGTAGCAATAAGCATGACCAGAGCGAACAACCATCgt > 1:449227‑M1/29‑251 (MQ=255) | AAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTTTGTTGATATTTTGATGGTAGCCAATCAGCTTAACCGAAGGGGCAGGAGGAGGACCACTCCAGCTCACATTTTGCAATATTTCCCCAGTCATGAGCCATTGACTACTTTTATATTCACCAACGTACACTGCGGATGGCATCATTATTTTTAAATTAAAAGTCGCAATATTACGCGAATCACCGTTGTAACCGTAGTAAACGTCAACGCTATGCCCGGCAGCCATAGCTTCTGTAGTAGCAATAAGCATGACCAGAGCGAACAACCATCGT > NC_000913/2449569‑2450038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |