Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,215	+C	intergenic (‑26/+23)	yjjA ← / ← dnaC	DUF2501 domain‑containing protein YjjA/DNA replication protein DnaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,214	1	.	C	100.0%	165.2 / NA	48	intergenic (‑25/+24)	yjjA/dnaC	DUF2501 domain‑containing protein YjjA/DNA replication protein DnaC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/22);  total (26/22)

CATTGTTCATGTTATCTGCGCTTAAGGCCTGGTTTCCGCTGCTAAGCAAGTTAGTTAATGACGCGAGCGACCAACCGCCTTCCTGTGTCGTGCTGTTTTGGTTGCCAAGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCG‑CAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTG  >  NC_000913/4599967‑4600456                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   ggtcgtgcgtgtggtcaccggtatcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttagggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaagtaacgaagaaagggagcgaaagctccctttattgtggttagcaaagtgtgcttcgttcattcctgaTGTGTcg‑cca                                                                                                                                                                                                                                                 <  1:296707‑M2/10‑3 (MQ=255)      tgcgtgtggtcaccggtatcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagctaaagctccctttagtattgatagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGtat                                                                                                                                                                                                                                              <  1:456972‑M2/14‑1 (MQ=255)                       atcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagatccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAacggggtt                                                                                                                                                                                                                            >  1:79393‑M2/220‑249 (MQ=255)                           gtttcggttttgttgtctccgtacgggaccagaccttcggcccgctgacgtgggtttatgccatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAAt                                                                                                                                                                                                                        <  2:166408‑M2/36‑1 (MQ=255)                               cggtttggtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATAct                                                                                                                                                                                                                     <  1:470881‑M2/39‑1 (MQ=255)                                      gtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCt                                                                                                                                                                                                             >  2:202986‑M2/205‑251 (MQ=255)                                                    ggaccagatcttcggcccgctgacgttggattatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCggctccg                                                                                                                                                                                               >  1:151191‑M2/191‑248 (MQ=255)                                                      accagatcttcggcccgctgacgttggtttatggcataccaccgctcacaggcgcactgttgccccgcaccagcttcttcttactcagcctcaggcagttcatgagaaaatcgtaaccgactgaacgaataaagggagcgcaagctccctttattattgttagcaaagcgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGt                                                                                                                                                                                             >  1:244642‑M2/189‑251 (MQ=255)                                                         atatcttcggcccgctgccgtgggtgtatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttctgcttaatcagcctgtggctgtgaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgctgcgttcattcctgaTGTGTCGCCAGTATACCGCGAGAAACGGGGATTAATGCTCTTTACCGGTTAGCCGGCTACGGTAGc                                                                                                                                                                                          <  2:189246‑M2/66‑1 (MQ=255)                                                          gatcttcggcccgctgacgttggtttatggcatcccgcagatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCt                                                                                                                                                                                         >  1:412958‑M2/185‑251 (MQ=255)                                                                cggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATccc                                                                                                                                                                                    >  1:336951‑M2/179‑250 (MQ=255)                                                                    cctctgacgttggtttatggcatcccgacgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagggtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGTCTACGGTAGCTATCCCAGTTg                                                                                                                                                                               <  1:294292‑M2/77‑1 (MQ=255)                                                                    ccgctgacgttggtttatggcatcccgccgatcatcggcgcactattgcccaccgccagcttcttcttaatcagcctgtggctgttaatgagcaaatcgtcaccgaatgaacgactaaagggcgcgcaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGCATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTg                                                                                                                                                                               >  1:218617‑M2/175‑251 (MQ=255)                                                                           cgttggtttatggcatcccgacgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGAtcc                                                                                                                                                                        >  1:143421‑M2/168‑250 (MQ=255)                                                                                           cccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAcc                                                                                                                                                        >  1:463327‑M2/152‑251 (MQ=255)                                                                                                   tcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgactgaacgcataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTAACCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGgcgc                                                                                                                                                 >  2:83326‑M2/144‑250 (MQ=255)                                                                                                           gcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaactcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTcc                                                                                                                                        >  2:50902‑M2/136‑251 (MQ=255)                                                                                                                    ccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGt                                                                                                                               <  1:266508‑M2/125‑1 (MQ=255)                                                                                                                      aagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATAACGCGTTc                                                                                                                             >  2:364443‑M2/125‑251 (MQ=255)                                                                                                                                gtcttcttaatcagcctgtggcggttaatgagaaaatcgtagccgaatgaacgaataaagggagctaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAAc                                                                                                                   <  2:17243‑M2/137‑1 (MQ=255)                                                                                                                                       taatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGt                                                                                                              <  1:348592‑M2/142‑1 (MQ=255)                                                                                                                                          tcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAtct                                                                                                         >  1:74717‑M2/105‑251 (MQ=255)                                                                                                                                                   ggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCata                                                                                                 <  1:130796‑M2/155‑1 (MQ=255)                                                                                                                                                             gagaaaatcgtaaccgcatgaacgaataaagggagcgtaagctaccttcataattgttagcaaagtgtgcttcgttcatccctgaTGTGTCGCCAGTCTACCGCCAGCAACGGGGATTACTCCTCTTTACCTGCTACCCGGCTATGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCCCGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGt                                                                                      >  1:463779‑M2/86‑251 (MQ=255)                                                                                                                                                               gaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGtt                                                                                                                                                                                                          >  2:326773‑M2/84‑133 (MQ=255)                                                                                                                                                               gaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGtt                                                                                                                                                                                                          <  1:326773‑M2/50‑1 (MQ=255)                                                                                                                                                                   atcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAACCGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCAt                                                                                >  1:243305‑M2/80‑251 (MQ=255)                                                                                                                                                                      gtaaccgaatgaacgaataaagggagcgaaagctccctttcttattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATccc                                                                             >  1:253372‑M2/77‑251 (MQ=255)                                                                                                                                                                      gtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCATCATccc                                                                             >  2:340838‑M2/77‑251 (MQ=255)                                                                                                                                                                        aaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAGACGGGTATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGTTCATCTCGTCCATAGTACTGTTGGTCAGCATCCCgg                                                                           <  1:399761‑M2/177‑1 (MQ=255)                                                                                                                                                                                        aaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGga                                                              >  2:157520‑M2/59‑248 (MQ=255)                                                                                                                                                                                          agggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaaga                                                           >  1:443174‑M2/57‑249 (MQ=255)                                                                                                                                                                                          agggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGaagaag                                                         <  2:350871‑M2/195‑1 (MQ=255)                                                                                                                                                                                               gcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAgcgg                                                    >  2:86471‑M2/52‑251 (MQ=255)                                                                                                                                                                                                       cccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATcgac                                            <  2:412958‑M2/208‑1 (MQ=255)                                                                                                                                                                                                       cccctttattattgttagcaaagtgtgcttcgtgcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCAGATTGCTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAATAAGAGCGGCGATcgac                                            <  2:443174‑M2/208‑1 (MQ=255)                                                                                                                                                                                                        ccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATcgac                                            <  1:8963‑M2/208‑1 (MQ=255)                                                                                                                                                                                                            ttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATc                                        <  1:202986‑M2/212‑1 (MQ=255)                                                                                                                                                                                                             tattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCt                                       <  2:151191‑M2/213‑1 (MQ=255)                                                                                                                                                                                                                  ttgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTtg                                  >  2:97941‑M2/33‑250 (MQ=255)                                                                                                                                                                                                                           aagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCCGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTc                                                                                     >  2:205449‑M2/24‑190 (MQ=255)                                                                                                                                                                                                                           aagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTc                                                                                     <  1:205449‑M2/167‑1 (MQ=255)                                                                                                                                                                                                                                 gcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGt                  >  1:328673‑M2/18‑251 (MQ=255)                                                                                                                                                                                                                                     cgttcattcctgaTGTGTCGCCAGTATCCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTGCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCAACACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTAttt              <  2:267664‑M2/238‑1 (MQ=255)                                                                                                                                                                                                                                     cgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCAGCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTAtt               <  1:479619‑M2/237‑1 (MQ=255)                                                                                                                                                                                                                                     cgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAAAGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTAttt              >  1:448782‑M2/14‑251 (MQ=255)                                                                                                                                                                                                                                          attcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGTCTACGGTAGCTATTCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTGCGAtt         <  2:133635‑M2/243‑1 (MQ=255)                                                                                                                                                                                                                                                 aTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTg  >  1:358162‑M2/2‑251 (MQ=255)                                                                                                                                                                                                                                                 aTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTg  <  1:289584‑M2/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   CATTGTTCATGTTATCTGCGCTTAAGGCCTGGTTTCCGCTGCTAAGCAAGTTAGTTAATGACGCGAGCGACCAACCGCCTTCCTGTGTCGTGCTGTTTTGGTTGCCAAGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCG‑CAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTG  >  NC_000913/4599967‑4600456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑