Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,008,439	(T)5→4	coding (621/621 nt)	rhtB ←	L‑homoserine/L‑homoserine lactone/L‑threonine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,008,439	0	T	.	100.0%	242.2 / NA	53	coding (621/621 nt)	rhtB	L‑homoserine/L‑homoserine lactone/L‑threonine exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (24/29);  total (24/29)

CTACCGCGCGCTGGGGCATTTTTGCGCT‑GATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCATGATGAATTGCGGAAATAGCGCCGCCAGAAACACAATACT  >  NC_000913/4008193‑4008687                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         cTACCGCGCGCTGGGGCATTTTTGCGCT‑GATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGCGTAAGTCGGATAAGGCGTTTACGCCGCATCCGCCATTATTTT‑CACGc                                                                                                                                                                                                                                                     >  2:3300/1‑251 (MQ=255)        gcgcTGGGGCATTTTTGCGCT‑GATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTc                                                                                                                                                                                                                                              <  1:203103/251‑1 (MQ=255)         cgcTGGGGCATTTTTGCGCT‑GATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCg                                                                                                                                                                                                                                             >  1:95684/1‑251 (MQ=255)            tGGGGCTTTTATCCGCT‑GATCATTGTCGACACGCTGGCGTGGATTACCGTCGTTGCACCCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGCCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCCTTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTAGCCg                                                                                                                                                                                                                                          >  2:213029/1‑251 (MQ=255)             ggggattttttGCGTG‑GATCATTGTCGAAACGCTGGCGTGGTTTCCCGCCGTTGCCAGCCTGTTTTCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTTCCGGATTTGGCATTCATTTGATTATTTCGCGGTGAGTCCATACTCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGcc                                                                                                                                                                                                                                           <  2:312610/243‑1 (MQ=255)             ggggCATTTTTGCGCT‑GATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTTGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGcc                                                                                                                                                                                                                                           <  1:276438/249‑1 (MQ=255)             ggggCATTTTTGC‑CTCGATCATTGTCGAAACGCTGGCGTGTTTTACCGGCGTTGCCGGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGcc                                                                                                                                                                                                                                           <  2:54249/249‑1 (MQ=255)              gggCATTTTTGCGCT‑GATAATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGAt                                                                                                                                                                                                                                        <  1:157531/251‑1 (MQ=255)                     tttGCGCT‑GATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTTATGGTTTTGCCGGGGCGTTAGTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGGGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCGCGCCGATGCTAAc                                                                                                                                                                                                                                  <  1:468447/250‑1 (MQ=255)                              gATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCACTGCCGCAAATGCGCCGTGGTTATCAATGTCTCGCGAAGTGGATTGATGGTTTTGCCCGGGAGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGCAGCGTCTTCAGAGTAAGACGGCTAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCTCCGCTGCTAACAGCGCTccc                                                                                                                                                                                                                         >  1:235081/1‑251 (MQ=255)                               atcatcGTCGAAACGCTGGCGCGGTTTACCGTCGTTGCCAGCCTGCTTGCCCTGCCGCAAATGCGCCGTGGCTATCAACGTCTCGCGAAGTGGATTGATGGATTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGTCGCGTCTTCAGAGTAAGTCGGATAAAGCGTTTACGTCGCATACGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTcccc                                                                                                                                                                                                                        >  2:248775/1‑250 (MQ=255)                               aTCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTGGCCGATGCTAACAGCGCTCcca                                                                                                                                                                                                                        <  1:94143/251‑1 (MQ=255)                                  aTTGTCAAAACGTTGGCGTGGTTTCCCGTCGTTGCCGTCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCccacc                                                                                                                                                                                                                      <  2:109028/250‑1 (MQ=255)                                                   gTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCg                                                                                                                                                                                                    >  1:242209/1‑251 (MQ=255)                                                          aCCGTCGTTGCCAGCCTTTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTGGCCGGGGCGTTATTTGCCGGATTGGGCATGCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTAGTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCt                                                                                                                                                                                             <  1:35036/251‑1 (MQ=255)                                                                   gactgcttGTTTGCCCTGCCGCAAGTGCGCCGTGGTTATCAACGTCTGGCTAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCTGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTCCAGAGTAAGTCTGATAATGCGTTTACGCCACATCCGACATTATTTT‑CACGCATGCCTCGTCGATGCTAACAGCGCACCCTTCACCATAAACATCGGCCCGAAAATCTTATTCAgc                                                                                                                                                                                     <  2:75065/243‑1 (MQ=255)                                                                      aGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCt                                                                                                                                                                                 <  1:260312/251‑1 (MQ=255)                                                                           gTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATc                                                                                                                                                                            >  1:232644/1‑251 (MQ=255)                                                                           gTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATc                                                                                                                                                                            >  2:191154/1‑251 (MQ=255)                                                                              tGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGc                                                                                                                                                                         >  1:331313/1‑251 (MQ=255)                                                                                       gCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTAGTCAGTGCCTTCATCTGCTTTGGTCCt                                                                                                                                                                <  1:388979/251‑1 (MQ=255)                                                                                           aTGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCCTTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTa                                                                                                                                                             >  1:301358/1‑250 (MQ=255)                                                                                                    ggTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATag                                                                                                                                                     >  1:80584/1‑249 (MQ=255)                                                                                                     gTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGc                                                                                                                                                  <  1:360677/251‑1 (MQ=255)                                                                                                     gTTATCAACGTCTGGCGAAGTGGATTGAGGGTTTTGGCGAGGCGTTAGTTGCCAGATTGGGAATACATGTGATTAACGCGTGGTTATGCCGGACGCGGCATCGGAGTAAGTCGGATAAGCCGTTTACGCCGGATCCGACAGTAGTTT‑CACGCAAGCCTTGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGGCCTTTAATCCATAGAGc                                                                                                                                                  <  2:10034/251‑1 (MQ=255)                                                                                                               tCTGGCGAAGTGGATTGATGGTTTTCCCGGGGCGATATTTGCCGGATTTCGCATTCATTTGATCATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTCCGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGCAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTg                                                                                                                                         >  1:234776/1‑250 (MQ=255)                                                                                                                   gCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCa                                                                                                                                     >  1:434393/1‑250 (MQ=255)                                                                                                                            aTTGATGGTTTGGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGGGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGt                                                                                                                           <  2:316924/251‑1 (MQ=255)                                                                                                                            aTTGATGGATTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGt                                                                                                                           >  1:89976/1‑251 (MQ=255)                                                                                                                                        gCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGAtcatca                                                                                                               <  1:248775/251‑1 (MQ=255)                                                                                                                                                   aTTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATc                                                                                                     <  1:333295/250‑1 (MQ=255)                                                                                                                                                     ttGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGAc                                                                                                  >  2:8652/1‑251 (MQ=255)                                                                                                                                                               tGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAgtg                                                                                        >  1:131838/1‑251 (MQ=255)                                                                                                                                                                  cattcataTGATTATTTCGCGGAGATGCCCGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGAATCCGACATTATTTT‑CACGCCTGCCTCGACGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAACTCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGCGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACAACAATAgtggtg                                                                                     >  2:422086/1‑251 (MQ=255)                                                                                                                                                                      cattTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGc                                                                                 <  1:8652/251‑1 (MQ=255)                                                                                                                                                                       attTGATTATTTCGCGTTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCAGCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGcc                                                                                <  2:88283/251‑1 (MQ=255)                                                                                                                                                                            attattTCGCGGTGATGCCAGACGCGTATTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGAAATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGcc                                                                           >  2:28408/1‑250 (MQ=255)                                                                                                                                                                                      ggTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGGTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTg                                                                 <  1:476447/251‑1 (MQ=255)                                                                                                                                                                                       gggATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGc                                                                <  2:356422/249‑1 (MQ=255)                                                                                                                                                                                          atgccaaactcttCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATc                                                             <  2:163225/239‑1 (MQ=255)                                                                                                                                                                                               aGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTg                                                        <  1:308919/251‑1 (MQ=255)                                                                                                                                                                                                       cTTCAGAGTAAGTCGGATAAGGCGTTAACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGGGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGtt                                                >  1:96680/1‑251 (MQ=255)                                                                                                                                                                                                           agagTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTg                                                        >  2:50879/1‑239 (MQ=255)                                                                                                                                                                                                           agagTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTGTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAAGAGTGGTGACGCCGAGCACGATATACTGCATCAGTTg                                                        <  1:50879/239‑1 (MQ=255)                                                                                                                                                                                                                 aGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCatgat                                      <  2:131838/251‑1 (MQ=255)                                                                                                                                                                                                                      gATAAGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGACAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGCGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCATGATGAAtt                                 >  1:474973/1‑251 (MQ=255)                                                                                                                                                                                                                          aGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGCTGCTACCAGCGCTCCCACCAGCATAAACAACGAGCCGAACATCTTATTCAGAGCCTTCCTCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAAGGTGGCGTACCCGATCATCCCAATAATATCGACGACCATCGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGGTGCGGCATGATGAATTGCgg                             >  1:229244/1‑251 (MQ=255)                                                                                                                                                                                                                          aGGCGTTTACGCCGCATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGGAtgatgacattcgg                             >  2:116938/1‑244 (MQ=255)                                                                                                                                                                                                                                  actccgcATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCGTCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATAGACGGCATCAGTTGCGGCTGTTGCGGCATGATGAATTGCGGAAATAgcg                     <  1:213029/248‑1 (MQ=255)                                                                                                                                                                                                                                   cgccgcATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCATGATGAATTGCGGAAATAgcgc                    >  1:118420/1‑251 (MQ=255)                                                                                                                                                                                                                                      cgcATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCATGATGAATTGCGGAAATAGcgccgc                 <  2:232644/251‑1 (MQ=255)                                                                                                                                                                                                                                       gcATCCGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCATGATGAATTGCGGAAATAGCgccgcc                <  1:76435/251‑1 (MQ=255)                                                                                                                                                                                                                                           ccGACATTATTTT‑CACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCATGATGAATTGCGGAAATAGCGCCGCCAg              <  2:95684/249‑1 (MQ=255)                                                                                                                                                                                                                                                 attattttCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCATGATGAATTGCGGAAATAGCGCCGCCCGAAacac        >  1:302178/5‑250 (MQ=255)                                                                                                                                                                                                                                                      tttCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCATGATGAATTGCGGAAATAGCGCCGCCAGAAACACAATACt  >  1:339866/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                         CTACCGCGCGCTGGGGCATTTTTGCGCT‑GATCATTGTCGAAACGCTGGCGTGGTTTACCGTCGTTGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGGTTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTGATGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGATGCTAACAGCGCTCCCACCAGCATAAACAACGAGCCGAAAATCTTATTCAGCGCCTTCATCTGCTTTGGTCCTTTAATCCATAGAGCAATCCGTTGAGCAAGGGTGGCGTAACCGATCATCACAATAATATCGACCACAATAGTGGTGACGCCGAGCACGATATACTGCATCAGTTGCGGCTGTTGCGGCATGATGAATTGCGGAAATAGCGCCGCCAGAAACACAATACT  >  NC_000913/4008193‑4008687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑