Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	380,022	(G)10→9	intergenic (‑141/+47)	frmR ← / ← yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,022	0	G	.	100.0%	119.2 / NA	29	intergenic (‑141/+47)	frmR/yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/11);  total (18/11)

GCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGACTGACGCTCTTCATGCTGCCGTAGCGTGTTTCTGGCGTCCAGTCATAGGTGTAAGCACCTGTTCCGCG  >  NC_000913/379777‑380266                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     gCATTCGGCATCCCCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGCGGAAAGGGGGGGGG‑CTAATc                                                                                                                                                                                                                                                >  2:397584/1‑251 (MQ=255)              cccTCCAGCGACCGTTCCAGAGCATCAAGCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGATTACTGGGCATTTCGCACCTCATCATCGGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATGCCCCCCTATAGGATAGAACACTGGAGAATAAAATTTATCCGGTGAATGTTGTGGGAGAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGgcc                                                                                                                                                                                                                                   <  1:213398/251‑1 (MQ=255)                          cGTTCCAGAGCATCAATCTGCCCCCGAAGACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATTTCAATGCAAACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGTGAAGGCCGCCCCGGATAgc                                                                                                                                                                                                                       <  1:294306/251‑1 (MQ=255)                              ccAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc                                                                                                                                                                                                                   <  1:23841/251‑1 (MQ=255)                                       cAATCTGCCCCCGAATACGACGACCTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgt                                                                                                                                                                                                          <  2:156884/251‑2 (MQ=255)                                              cccccGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCTGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                                                                                                                                                                                      >  1:34028/1‑248 (MQ=255)                                               ccccGAATACGAAGAACTCGCGTACGGACCTTTTTCTTTTCTTCCGGAGCACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGCGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACCAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCTGGAGAAGGAAACAgt                                                                                                                                                                                                   >  2:270148/1‑249 (MQ=255)                                                       acgacgAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGTAAAACAAAGAGTAAAGGGGGGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                                                                                                                                                                                         <  1:220023/251‑1 (MQ=255)                                                                     tAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCCTCATCTGCATGCAATATACTATAGGGGGGAATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGCAGGAATCAGAATTTCCAGGGCAGACGGGCTGc                                                                                                                                                                             >  2:253611/1‑250 (MQ=255)                                                                                    tctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCCTGCCATATACTATAGGGGGGTATTCTATATGTCAACACATACCCCCCTATAGCATATAACACTGGAGCATAACATTTATCCGGTGAATGCGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGCCAGACGGGCTGCAAGTTGCAGCCCGtt                                                                                                                                                              >  1:435878/1‑250 (MQ=255)                                                                                          ccGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATTCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAGTTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatca                                                                                                                                                       <  1:317220/251‑1 (MQ=255)                                                                                               gTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                                                                                                                                                  >  1:261663/1‑251 (MQ=255)                                                                                                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTACAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTggggg                                                                                                                                            >  1:310408/1‑250 (MQ=255)                                                                                                                ccTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACcac                                                                                                                                  >  1:302219/1‑250 (MQ=255)                                                                                                                      catcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc                                                                                                                          <  1:9552/251‑1 (MQ=255)                                                                                                                           gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGcc                                                                                                                      <  1:211640/250‑1 (MQ=255)                                                                                                                                         ataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGAt                                                                                                         <  1:397750/250‑1 (MQ=255)                                                                                                                                              ggggTCTTCTATATGTCCATGCATACCACCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGCAGCCCGCCCGGGATAGCGTGCGGCAGAAGGAATAAGAATTTCCAGGTCAGACGTGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTAGTACCACACTTTACCTGCCGTCAGTCCGAGATTACGtt                                                                                                   >  2:278195/1‑251 (MQ=255)                                                                                                                                              ggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGtt                                                                                                   >  1:334829/1‑251 (MQ=255)                                                                                                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGCCAGCCCGGGATTAAGTTGCccagtc                                                                                           >  2:127328/1‑250 (MQ=255)                                                                                                                                                                  gCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGCTTAAGTTGCTCAGTCAGCGGTTGaac                                                                                >  2:277361/1‑249 (MQ=255)                                                                                                                                                                            cTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGCATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTCAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGAc                                                                     >  1:316639/1‑251 (MQ=255)                                                                                                                                                                              ataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGCCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGGAGAAGGAATCAGAATTTCCAGGTCAGCCGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTCCCACATTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGCCTg                                                                   >  2:23439/1‑251 (MQ=255)                                                                                                                                                                                aGTATATAACACTGGAGCATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGG‑‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCCGTCAGCGGTTGAATACGTTGCCGACTGACGc                                                               >  2:38875/1‑251 (MQ=255)                                                                                                                                                                                             ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCAGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGGAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAc                                                                                                                                     >  2:155413/1‑170 (MQ=255)                                                                                                                                                                                                    aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTACTCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGCAGGCATCAGAATTTCCCGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGGTGAATACGTTGCAGACTGACGCTCTTCATGCTGCCGTAGc                                             >  1:189443/1‑251 (MQ=255)                                                                                                                                                                                                                  gAATGGGGGCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGACTGACGCTCTTCATGCTGCCGTAGCGTGTTTCTGGCGTc                               <  2:228844/251‑1 (MQ=255)                                                                                                                                                                                                                        ggTCGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGACTGACGCTCTTCATGCTGCCGTAGCGTGTTTCTGGCGTCCAGTc                          >  1:174100/1‑250 (MQ=255)                                                                                                                                                                                                                         ggcGGAAAACAAAGAGGAAAGGGGGGGGG‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGACTGACGCTCTTCATGCTGCCGTAGCGTGTTTCTGGCGTCCAGTCAt                        <  2:334241/249‑1 (MQ=255)                                                                                                                                                                                                                                          gaaagggggggggCTAATCGGCAGGGAAGGCCGCCCCGGACAGCGGGAGGCAGAAGGAATCAGAATTTCCAGGCCAGACGGGCAGCAAGTTGCAGCACGTTAAAATCATCGGTTGGGGTATCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGACTGACGCTCTTCATGCTGCCGTAGCGTGTTTCTGGCGTCCAGTCATAGGTGTAAGCACCTGt        >  1:471572/5‑251 (MQ=255)                                                                                                                                                                                                                                             gtggggggggCCAATCGGCAGGGAACGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGCCGGGCGGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTGACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTGGCAGACTGACGCTCTTCATGCTGCCGTAGCGTGTTTCTGGCGTCCAGTCATAGGGGTAAGCACCTGTTcc     <  2:58269/249‑1 (MQ=255)                                                                                                                                                                                                                                                gggggggCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGACTGACGCTCTTCATGCTGCCGTAGCGTGTTTCTGGCGTCCAGTCATAGGTGTAAGCACCTGTTCcgcg  >  2:265475/1‑251 (MQ=255)                                                                                                                                                                                                                                                gggggggCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGACTGACGCTCTTCATGCTGCCGTAGCGTGTTTCTGGCGTCCAGTCATAGGTGTAAGCACCTGTTCcgcg  >  1:300500/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     GCATTCGGCATCACCCTCCAGCGACCGTTCCAGAGCATCAATCTGCCCCCGAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCAGCCCGAGATTAAGTTGCTCAGTCAGCGGTTGAATACGTTGCAGACTGACGCTCTTCATGCTGCCGTAGCGTGTTTCTGGCGTCCAGTCATAGGTGTAAGCACCTGTTCCGCG  >  NC_000913/379777‑380266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑