Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,174,041	A→T	E320D (GAA→GAT)	birA →	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,174,041	0	A	T	100.0%	205.5 / NA	55	E320D (GAA→GAT)	birA	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (30/25);  total (30/25)

GGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCGGTAAAAGCCCCTT  >  NC_000913/4173798‑4174281                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 ggcCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATa                                                                                                                                                                                                                                             >  1:258143/1‑249 (MQ=255)      tGCTAATCCGTCAATTAAGAGCTGCGTTGCAACTATTCGAACCAGATGGGCTGGAACCTTATCTGTCGCGTTGGGAAAACCTGGATAATTTTATTAATCGCACAGTGAAACTTATCATTGGTGATCAAGAAATATTTGGCATTTCCTGCGGAATAGACACACAGGGGGCTTTATTACTTGAGCAGGCTGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATCACTAAGAAAgg                                                                                                                                                                                                                                      >  2:301611/1‑251 (MQ=255)        cTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAgggg                                                                                                                                                                                                                                    >  2:573488/1‑251 (MQ=255)           aTACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATTGGCGGTTAAATATCCCTGCTGAGTGCAGATAAATAAGAAAGGGGAGt                                                                                                                                                                                                                                 <  1:280344/251‑1 (MQ=255)               gTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTc                                                                                                                                                                                                                             <  2:49650/251‑1 (MQ=255)                 gAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATGGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGTGCTTTATTACTGGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGGTAAATAAGAAAGGGGAGTATTCg                                                                                                                                                                                                                            <  2:317076/250‑1 (MQ=255)                      aCGTGCTGCGTTGCAACTCTTCGAACAAGCCGCTTTCGCAACTTCTCTGACGCGCTGGGAACAGCTGGATAATTTTATTAATCGCCCAGTGACACTTATCATTGGTGATAAAGAAATCTTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAgggatgacgaaatcatgc                                                                                                                                                                                                                      >  2:336419/1‑236 (MQ=255)                               gTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAatta                                                                                                                                                                                                             <  1:74622/251‑1 (MQ=255)                                  ggAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAattattg                                                                                                                                                                                                          <  2:499971/251‑2 (MQ=255)                                        ctTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGACAAGCTGGCTAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGACATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGt                                                                                                                                                                                                      >  2:507342/1‑249 (MQ=255)                                         ttCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGt                                                                                                                                                                                                   >  1:7985/1‑251 (MQ=255)                                           cGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTc                                                                                                                                                                                                  <  1:370423/250‑1 (MQ=255)                                           cGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCt                                                                                                                                                                                                 <  1:402990/251‑1 (MQ=255)                                            gAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAACCTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAatt                                                                                                                                                                                                              >  2:712668/1‑237 (MQ=255)                                            gAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAatt                                                                                                                                                                                                              <  1:712668/237‑1 (MQ=255)                                                     ggATTGGCAACTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGGGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTTAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGTAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTTGCTCCCCTGCAAATTATTTGCGTAGTTTGACCtcttct                                                                                                                                                                                       <  2:104655/251‑1 (MQ=255)                                                                 tATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGAtt                                                                                                                                                                            >  2:582783/1‑250 (MQ=255)                                                                      gTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACt                                                                                                                                                                      <  2:258143/251‑1 (MQ=255)                                                                              gggAAAAGCTGGATACTTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTc                                                                                                                                                               >  1:212554/1‑250 (MQ=255)                                                                                gAAAAGCAGGAAAATTTTATTCATCGCCCGGTGAAACTTATCATTGGCGCTATAGAACTCTTAAGCACTTCACCCCGAATTGACAAACCGGCGGCTTTATTACTTGAGCAGGATGGAATCATAAACCACTGGCTGGGCGGTGAAATAACCCTGCGTAGCGCAGATAAGTAAGAAAGGGGAGTATCCGCTCCTCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGACTAGCACTTTTCGTccgg                                                                                                                                                            >  2:388828/1‑248 (MQ=255)                                                                                           tAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgc                                                                                                                                                 >  2:135201/1‑251 (MQ=255)                                                                                                    taATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACg                                                                                                                                        >  1:110493/1‑251 (MQ=255)                                                                                                    taATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGGAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACg                                                                                                                                        >  2:283018/1‑251 (MQ=255)                                                                                                     aaTCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACGTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGa                                                                                                                                       <  1:349089/251‑1 (MQ=255)                                                                                                        cGCCCAGTGAAACTTATCATTGGTTATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAAGATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCGCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTGCGTCAGGATTAAACTTGCGCGCTCACGAgta                                                                                                                                    <  2:504186/251‑1 (MQ=255)                                                                                                        cGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACCGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCAc                                                                                                                                         >  2:87551/1‑246 (MQ=255)                                                                                                        cGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCAc                                                                                                                                         <  1:87551/246‑1 (MQ=255)                                                                                                             aGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCAGGTCACGCGGAATAGACAAACAGGGTGCTTTATTACTTGAGCAGGATTGAATAATAAAACCCTGGATGGGCGGTGAAATAGCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTATTCTGACCTCTTCTACCGCATTATTAGCGCGTTTCGTCAGGATTAAACTATCTCGCCCTCGAgtaggtag                                                                                                                               <  2:367972/251‑1 (MQ=255)                                                                                                               tGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGa                                                                                                                              >  2:238178/1‑250 (MQ=255)                                                                                                                  aaCTTATCATTTGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAatat                                                                                                                          <  2:291741/251‑1 (MQ=255)                                                                                                                            tGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACCAACAGGGGGCTTTATTACTTGAGACGGCTGGAATAATCAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATCAATCAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGCGTAGGTAGAATATTTTGCTTTa                                                                                                                 >  1:329506/1‑250 (MQ=255)                                                                                                                                                 cATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTGAAGTGCAGCCAGTTGATCTCttt                                                                                           <  1:583047/251‑1 (MQ=255)                                                                                                                                                      cACGCGGAATCGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCcac                                                                                       >  2:681604/1‑250 (MQ=255)                                                                                                                                                      cACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAGGTGCAGCCAGTTGATCTCTTTCcaca                                                                                      <  1:283018/251‑1 (MQ=255)                                                                                                                                                        cgcgGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAAt                                                                                    >  2:564363/1‑251 (MQ=255)                                                                                                                                                        cgcgGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAAt                                                                                    >  2:564340/1‑251 (MQ=255)                                                                                                                                                         gcgGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACGATGGATGGGCGGTGAACTATCCCTGCGTCGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAGTTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCCGGATTAACCTGTCGCGCTCACGAGTTGGTAGAATATTTTGCTTTAAGTTCAGCAAGTTGATCTCTTTCCACAATg                                                                                   >  2:642900/1‑251 (MQ=255)                                                                                                                                                                 gACAACCAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGc                                                                            >  2:231194/1‑250 (MQ=255)                                                                                                                                                                 gACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGCTGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTg                                                                                                   >  2:481249/1‑227 (MQ=255)                                                                                                                                                                 gACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTg                                                                                                   <  1:481249/227‑1 (MQ=255)                                                                                                                                                                    aaaCAGGGGGCTTTATTACTTGAGCAGGTTGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTTCCCTGCAAATTATTTGCGTAGGCTTGCCTCGGCTACCGGATGATTAGCACTTTTCGTCAAGATTAAACTGGCGCTCTCACGAGTAGGTAGAATATTTTGCTGTGAGTTCAGCCAGTTTATCTCTTTCGACAATGGCATGGCAGTc                                                                        <  2:651654/251‑1 (MQ=255)                                                                                                                                                                                        tGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTCCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAg                                                     <  1:582783/250‑1 (MQ=255)                                                                                                                                                                                                  ggAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTcgc                                           <  2:110493/250‑1 (MQ=255)                                                                                                                                                                                                     ataataAAACCCTGGATGGGCGGTGCAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGt                                         >  1:581424/1‑249 (MQ=255)                                                                                                                                                                                                          aaaaCCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                                  >  1:321628/1‑251 (MQ=255)                                                                                                                                                                                                                cTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGACAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAAt                             >  2:534290/1‑250 (MQ=255)                                                                                                                                                                                                                   gATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGCAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                                  >  1:733583/1‑242 (MQ=255)                                                                                                                                                                                                                   gATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCAGGGCAGGCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                                  <  2:733583/242‑1 (MQ=255)                                                                                                                                                                                                                    aTGGGCGGTGAAATATCCATGCGTAGTGCAGATAAATAAAAAAGGGGAGTCTTCGCTCCCCTGCCAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGGAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGa                         >  2:85873/1‑250 (MQ=255)                                                                                                                                                                                                                      gggCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCTTAGTTATGAAAATAGGAAt                       <  1:507342/250‑1 (MQ=255)                                                                                                                                                                                                                         cGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCgg                   >  1:508647/1‑251 (MQ=255)                                                                                                                                                                                                                                atagcccTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCGTCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATGAGCACTTTTCGTCAGGATTAACCTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTGCAGCCAGTGGATCTCTTTCCACAGTGGCATGGCAGGCTTAATCGCTTCGGCTTTAGCTAATTGCTCGTAGTTATGAAAAGAGGAATCCggtgcggta            <  2:448328/247‑8 (MQ=255)                                                                                                                                                                                                                                        gCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCACATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTc                                                                                 >  2:160224/1‑174 (MQ=255)                                                                                                                                                                                                                                        gCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAGCTCTTTCCACAATGTc                                                                                 <  1:160224/174‑1 (MQ=255)                                                                                                                                                                                                                                          gTAGTGCAGATAAATAAGAAAGGGGAGCATTCGCTGCCCTGAACATTATTTGCGTAGTCTGACCTCTTCTCCCGCATGCTTAGCACTTTTCGTCAGGATTAAACTGGCGAGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGATGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAACTAGGAATCCGGGTCGGTAAAAGCCCCtt  >  2:504269/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 GGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCGGTAAAAGCCCCTT  >  NC_000913/4173798‑4174281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑