BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,809	A→G	K252R (AAA→AGA)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,809	0	A	G	100.0%	383.4 / NA	101	K252R (AAA→AGA)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (47/54); total (47/54)

Only 100 of 101 total aligned reads displayed.

TGGCGAACAGCGTACGCGCTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGA  >  NC_000913/498569‑499053

tGGCGAACAGCGTACGCGCTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCCGCGTTCTGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGCT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCggg                                                                                                                                                                                                                                             >  1:333825/1‑250 (MQ=255)

 ggCGAACAGCGTACGCGCTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGgag                                                                                                                                                                                                                                            <  2:532586/251‑1 (MQ=255)

    gAACAGCGTACGCGCTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCTGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGaaa                                                                                                                                                                                                                                         <  1:65744/251‑1 (MQ=255)

      aCAGCGTACGCGCTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGaaaaa                                                                                                                                                                                                                                       <  2:134042/251‑1 (MQ=255)

      aCAGCGTACGCGCTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGaaaa                                                                                                                                                                                                                                        >  2:349502/1‑250 (MQ=255)

      aCAGCGTACGCGCTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGaaaa                                                                                                                                                                                                                                        >  1:491584/1‑250 (MQ=255)

           gTACGCGCTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGt                                                                                                                                                                                                                                  <  2:267660/251‑1 (MQ=255)

                cgcTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGAGGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTc                                                                                                                                                                                                                             <  1:334779/251‑1 (MQ=255)

                  cttcttTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGAGTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGTCCTTTCAGTCGCGCTTTTTTCGGGAACCCTGGCGGATGCCTGATACCTACGAAACGCTGAGAAGGCTCGGAGAAAAATGCGTAGGTc                                                                                                                                                                                                                             <  2:607391/249‑1 (MQ=255)

                   ttcttTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCat                                                                                                                                                                                                                           >  1:252159/1‑250 (MQ=255)

                    tcttTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCatat                                                                                                                                                                                                                         >  2:196647/1‑251 (MQ=255)

                    tcttTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCat                                                                                                                                                                                                                           <  2:596949/249‑1 (MQ=255)

                       tttGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTc                                                                                                                                                                                                                       >  2:376748/1‑250 (MQ=255)

                       tttGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTc                                                                                                                                                                                                                       <  2:546248/250‑1 (MQ=255)

                             aaaCATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGAt                                                                                                                                                                                                                >  2:594521/1‑251 (MQ=255)

                               aCATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCCTTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGAtgt                                                                                                                                                                                                              >  1:432291/1‑251 (MQ=255)

                                cATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGAtgtg                                                                                                                                                                                                             <  1:571992/251‑1 (MQ=255)

                                   ggCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGccc                                                                                                                                                                                                          <  1:253110/251‑1 (MQ=255)

                                   ggCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGcc                                                                                                                                                                                                           <  1:503023/250‑1 (MQ=255)

                                       aaCCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGc                                                                                                                                                                                                      <  2:494423/251‑1 (MQ=255)

                                          cGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCttt                                                                                                                                                                                                   <  1:570281/251‑1 (MQ=255)

                                              tCTGCCACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGACGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGCGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTACTGTGCCCGGGCTTtgctg                                                                                                                                                                                               >  2:50473/1‑251 (MQ=255)

                                                 gCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTtgctgc                                                                                                                                                                                              <  2:191591/249‑1 (MQ=255)

                                                 gCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCgg                                                                                                                                                                                            >  2:558501/1‑251 (MQ=255)

                                                       gCTCTCTTATCATGGCATTCCCCAGCGTTAGGCAGATGAAGGCGATGATTACCCGCAACGGTGGCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTGTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCGGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTg                                                                                                                                                                                        <  1:479444/249‑1 (MQ=255)

                                                              tATCATGGCATTCCCCAGCGTTATGCCGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTtgctgc                                                                                                                                                                                              >  1:262183/1‑236 (MQ=255)

                                                              tATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGCTTACCCGCAACGTTGCCGCACAAAGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAACCGCTGAGAATGCTCGGAGAAAAAGGCGAAGGTCATCTTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTATGGAGAc                                                                                                                                                                               >  2:479245/1‑251 (MQ=255)

                                                              tATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTtgctgc                                                                                                                                                                                              <  2:262183/236‑1 (MQ=255)

                                                                     gCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTCCCCGCCACGTTGCCGCACAACGAATCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGAGCT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCCGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGa                                                                                                                                                                         >  1:459912/1‑250 (MQ=255)

                                                                     gCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGa                                                                                                                                                                         >  1:146589/1‑250 (MQ=255)

                                                                      cATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGa                                                                                                                                                                         >  1:395539/1‑249 (MQ=255)

                                                                         tCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTGTGGTCGGAAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGAGGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAagag                                                                                                                                                                     <  1:481703/250‑1 (MQ=255)

                                                                               gCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCg                                                                                                                                                              >  2:581319/1‑251 (MQ=255)

                                                                                  ttATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGCCTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGCAAAAGCGCT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGc                                                                                                                                                           >  2:304205/1‑251 (MQ=255)

                                                                                   tATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGc                                                                                                                                                           <  1:397132/250‑1 (MQ=255)

                                                                                   tATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGc                                                                                                                                                           <  1:376748/250‑1 (MQ=255)

                                                                                      gCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCaaaa                                                                                                                                                       <  1:26136/251‑1 (MQ=255)

                                                                                      gCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCaaaa                                                                                                                                                       <  1:196647/251‑1 (MQ=255)

                                                                                      gCAGATAAAGGCGATGTTTACCCGCAACGTTGCCGCACACAGACTCGTGAAAAGGATTCCGCATTGCCGATGACAACGGCAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGAAGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGGCACATTCAGGGTATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGTGATTGCCGCGCaaa                                                                                                                                                        >  2:470850/1‑250 (MQ=255)

                                                                                                  gatgatTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCtt                                                                                                                                           >  1:469963/1‑251 (MQ=255)

                                                                                                      atTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGCGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTc                                                                                                                                       >  2:491710/1‑251 (MQ=255)

                                                                                                      atTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTc                                                                                                                                       <  2:603631/251‑1 (MQ=255)

                                                                                                        tACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCgg                                                                                                                                     >  2:538637/1‑251 (MQ=255)

                                                                                                         aCCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGt                                                                                                                                    >  2:252280/1‑251 (MQ=255)

                                                                                                            cGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGcc                                                                                                                                 <  1:349502/251‑1 (MQ=255)

                                                                                                            cGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGcc                                                                                                                                 <  1:581319/251‑1 (MQ=255)

                                                                                                                  gTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCggg                                                                                                                           <  1:281403/251‑1 (MQ=255)

                                                                                                                     gCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaa                                                                                                                        <  2:395539/251‑1 (MQ=255)

                                                                                                                     gCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGGCTTCCTCGGTGCCGGCGGGaaa                                                                                                                        <  2:101939/251‑1 (MQ=255)

                                                                                                                      ccGCACAACGACCCGTGAACTGGCTGCCGCATTGGGGATGGCACCGGAAAAAGTGATTGA‑GACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCCTTGCTGCGGATTGTCAGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGaaaa                                                                                                                       <  2:491584/251‑1 (MQ=255)

                                                                                                                        gCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGgaaaa                                                                                                                      >  1:488461/1‑250 (MQ=255)

                                                                                                                          ccaACGACTCGTGAACTGGCTTCCGCTATGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGAGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGCGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATAGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGTGAAAAAata                                                                                                                   <  2:109862/250‑1 (MQ=255)

                                                                                                                          acaACGACTCGTGCACTGGCTTCCGCATTGGGGATGGCACCGAAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCATGGCTGATGCCTTATCCCGACGAAAAGCTGAGAATGCTCGGAGAAACAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGCTTGTCTGGAGACGCTGGAAGAGCTTGCCGAGCAAAACCTTGAGGTCTTCCTCGGTGCAGGCggggagaaat                                                                                                                    >  2:673195/1‑243 (MQ=255)

                                                                                                                           caACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCCCCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAatat                                                                                                                  >  1:674318/1‑251 (MQ=255)

                                                                                                                            aaCGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCg                                                                                                                                                    <  1:297563/216‑1 (MQ=255)

                                                                                                                            aaCGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCg                                                                                                                                                    >  2:297563/1‑216 (MQ=255)

                                                                                                                            aaCGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATg                                                                                                                 >  1:163465/1‑251 (MQ=255)

                                                                                                                              cGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGCCCTTTCAGTCGCGCTTTGGCCGGGCACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAACGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGa                                                                                                                >  1:652676/1‑250 (MQ=255)

                                                                                                                               gACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCTAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGTTGGAAAAAATATGAat                                                                                                              <  2:432291/251‑1 (MQ=255)

                                                                                                                               gACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTGTCAGTCGCGCTTGGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCGTCCGCGGTGCCGGCGGGAAAAAATATGAat                                                                                                              <  2:319643/251‑1 (MQ=255)

                                                                                                                                 cgcGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGTCGGGAAAAAATATGAatat                                                                                                            <  1:639412/249‑1 (MQ=255)

                                                                                                                                    gTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAACCGAT‑GATGCCATTTCAGTCGCGCTTTGGACGAGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCCCGGAGAACAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGACGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATTAACATAtt                                                                                                         >  1:311003/1‑251 (MQ=255)

                                                                                                                                               tCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAAGATATTCCGGCGCTTa                                                                                               <  1:248305/250‑1 (MQ=255)

                                                                                                                                                       ggggATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGTGACAAAATGAATAAATTCTGGGGCTTAATGCCCCGc                                                                                      >  2:179692/1‑251 (MQ=255)

                                                                                                                                                       ggggATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACg                                                                                       <  1:538637/250‑1 (MQ=255)

                                                                                                                                                        gggATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATAAGAATATATTTCGGCGCTTAatgccatgcc                                                                                     >  1:269294/1‑251 (MQ=255)

                                                                                                                                                         ggATGGCACCGGAAAAAGGGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATGCAGGGGATGTGCCCGGGCTTTGCTGCGGATGTTCTGGAGACGCTGGAAGAGATTGCCGGTCAAAGCCTGGAGGTCTTCCTGGGTGCGGGCTGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCg                                                                                    <  1:379594/251‑1 (MQ=255)

                                                                                                                                                               cACCGGAAAAAGTGAT‑GATGTCCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACat                                                                              <  2:214804/251‑1 (MQ=255)

                                                                                                                                                                 ccGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACatat                                                                            <  1:206270/251‑1 (MQ=255)

                                                                                                                                                                    gAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCGCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTTCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTGCCTCGGTGCCGGCTGGAAAAAATATGAATATATTCCGGCGATTAATGCCACGCCGGAACATATCGa                                                                         <  1:351644/251‑1 (MQ=255)

                                                                                                                                                                        aaGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTTCGGCGCTTTATGCCACGCCGGGACATATCGAAatg                                                                     >  1:59673/1‑251 (MQ=255)

                                                                                                                                                                         aGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTTCGGCGCTTAATGCCACGCCGGAACATATCGAAatg                                                                     >  1:231245/1‑250 (MQ=255)

                                                                                                                                                                         aGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatga                                                                    <  1:304205/251‑1 (MQ=255)

                                                                                                                                                                         aGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatga                                                                    <  2:146589/251‑1 (MQ=255)

                                                                                                                                                                         aGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATAAGAATATTTTCCGGCGCTTATAGCCACGCCGGAAAATATctgaa                                                                       >  2:179695/1‑244 (MQ=255)

                                                                                                                                                                          gTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAatgat                                                                   <  1:252280/251‑1 (MQ=255)

                                                                                                                                                                            gat‑gatgaCCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACAGATCGAAatgatg                                                                  <  1:179692/250‑1 (MQ=255)

                                                                                                                                                                               ggatgaCCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCTAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACAGATCGAAATGATGG‑Ct                                                              <  1:179268/250‑1 (MQ=255)

                                                                                                                                                                                   gaCCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTAGACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTTGAAGAGATTGCCGAGCAAAACCGTGAGGTCTGCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGTTGCTTAATGCCTCGCCGGAACATATCGAAATGATGG‑CTAAt                                                           <  1:50473/250‑1 (MQ=255)

                                                                                                                                                                                    aCCTTTCAGTCGCGATTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGCAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAAAATATTTCGGCGCTTAATGGCACGCCCGGACATATCGAAATGATGG‑Cttattt                                                         >  2:701193/1‑246 (MQ=255)

                                                                                                                                                                                       tttCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAACAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTACTCGGTGCCGGCGGGGAAAAATATGAAAATATTTCGGGGCTTTTTGCCACGCCGGGCAATATCGAAAAGTTGG‑GTTATCttgt                                                      >  2:707760/1‑251 (MQ=255)

                                                                                                                                                                                        ttCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGAGGG‑CTAATCttgt                                                      <  2:469963/250‑1 (MQ=255)

                                                                                                                                                                                          cAGTCGCGCTTTGGTCGGGAACCATGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTGc                                                   <  1:452072/251‑1 (MQ=255)

                                                                                                                                                                                            gTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTGCcg                                                 <  2:674318/251‑1 (MQ=255)

                                                                                                                                                                                                 gcgttGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTGCCGCGTAt                                            <  1:94831/248‑1 (MQ=255)

                                                                                                                                                                                                 gcTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTGCCGCGTAt                                            <  1:558501/251‑1 (MQ=255)

                                                                                                                                                                                                 gatttGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGT‑CTAATCTTGTTGCCGCGTAt                                            <  1:239408/249‑1 (MQ=255)

                                                                                                                                                                                                  cTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTTCCGCGTATc                                           >  1:631168/1‑251 (MQ=255)

                                                                                                                                                                                                      ggTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTGCCGCGTATCGCTa                                       <  1:491710/251‑1 (MQ=255)

                                                                                                                                                                                                        tCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGACGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAAAATATTTCGGGGCTTTATGCCACGCCGGAACATAACGCAAAGATGG‑CTAATCTTGTTGTcgcggatatccaa                                      >  2:60670/1‑241 (MQ=255)

                                                                                                                                                                                                            gAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAGAGTGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATAAATTCCGGCGCCTTATGCCACGCCGGAACATAACGAAATGATGG‑GTAATCTTGTTTCCGCGGATTGCTAAAGccg                                 >  2:583019/1‑249 (MQ=255)

                                                                                                                                                                                                                           ccTTATACCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGGACATATCGAAAAGATGG‑CTAATCTTGTTGCCGCGGATCGCTAAAGCTGAGCGGTAAAGaaatg                  >  1:93890/1‑248 (MQ=255)

                                                                                                                                                                                                                              tataCCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTCTCGCGGTTAATTCCCCGCCGGGACACATAGAAATGATGGGCTA‑TCTTGTTGCCGCGGACCGCTAAAGCTGGACGGGAAAGaaatgggg               >  2:250859/1‑245 (MQ=255)

                                                                                                                                                                                                                                 aCCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGCTGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAggcc                                                                                                                                             >  2:318661/1‑121 (MQ=255)

                                                                                                                                                                                                                                 aCCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTc                                                                                                                                             <  1:318661/123‑1 (MQ=255)

                                                                                                                                                                                                                                 aCCGACGAAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTTCGGCGCCTAATTGCACGCCGGGACATCACGAAATTATGG‑CTCATCTTGTTGCCGCCTATCGCTAAAACTTAGAGGTAAAAAACTGAACGcc            >  1:569554/1‑251 (MQ=255)

                                                                                                                                                                                                                                   cgacgaAACGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAAAATATTTCGGCGCTTAATGCCCCGCCGGGACAAATCGGAATGATTG‑CTAATCTTGTTGGCGCGTATCGCTCAAACTGAGCGGGaaaaaaatgggggccgg          >  2:545811/1‑237 (MQ=255)

                                                                                                                                                                                                                                    gacgaAACGCTGAGAATGCTCTGAGAAAAAGGCGTAGGTCATATTCAGGTGAGGTGCCCGGGCTTTGCGGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGGCGTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTGGCGCGTATCGCTAAAGCTGAGCGGGAAAGAACTGAGGGCCGTc         <  2:333825/251‑1 (MQ=255)

                                                                                                                                                                                                                                          aCGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGTTGTGCCCGGGCTTTGCTGCGGATTGCCTGGAGACGCTGGAAGAGATTGCCGAGCAACACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCCGCGCTTAATGGCACGGCGGAACATATCGAAATTAATG‑CCAATCTTTTTGACGCGTCTCGCTAAAACATAGCGGCAAAGAAATGGGCGCCgacccga     >  1:315131/1‑243 (MQ=255)

                                                                                                                                                                                                                                           cGCTGAGAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTTCCGCGCTTTATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTGCCGCGGATCGCTAAAACTGAGCGGGAAAGAACTGAGCGCCGTCGCGAagg  >  1:663786/1‑250 (MQ=255)

TGGCGAACAGCGTACGCGCTTCTTTTGCCAAACATGGCGAACCGGATCTGCTACTGCTCTCTTATCATGGCATTCCCCAGCGTTATGCAGATGAAGGCGATGATTACCCGCAACGTTGCCGCACAACGACTCGTGAACTGGCTTCCGCATTGGGGATGGCACCGGAAAAAGTGAT‑GATGACCTTTCAGTCGCGCTTTGGTCGGGAACCCTGGCTGATGCCTTATACCGACGAAACGCTGAAAATGCTCGGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGG‑CTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGA  >  NC_000913/498569‑499053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`