Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,393	G→A	intergenic (‑75/+20)	glyQ ← / ← ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,393	0	G	A	100.0%	241.0 / NA	63	intergenic (‑75/+20)	glyQ/ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/41);  total (22/41)

AAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAAC  >  NC_000913/3725147‑3725606                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                      aaGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAAc                                                                                                                                                                                                                   <  2:216423/251‑1 (MQ=255)           cATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGGGGATTTAAAGGAATGGATCAGTATGCCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGc                                                                                                                                                                                                         <  1:697322/251‑1 (MQ=255)               ggTTCTGGCCGCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGAGATACAGTACAAAACGGGAAAAAGCAggg                                                                                                                                                                                                     <  2:603552/251‑1 (MQ=255)                gTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGc                                                                                                                                                                                                    <  1:312512/251‑1 (MQ=255)                     ggCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAAc                                                                                                                                                                                               >  1:230170/1‑251 (MQ=255)                     ggCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGAAGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAAc                                                                                                                                                                                               >  2:129672/1‑251 (MQ=255)                           aGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAATCAGGGCTTAACGCATgg                                                                                                                                                                                         <  1:618425/251‑1 (MQ=255)                                gcgcAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTGCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGTGAAAAGGCAAGTCCGAACGCATGGAAAGa                                                                                                                                                                                    <  2:309875/251‑1 (MQ=255)                                                gggTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATCCCCGCTGGAAGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCa                                                                                                                                                                     <  1:129672/250‑1 (MQ=255)                                                gggTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCa                                                                                                                                                                     <  2:628213/250‑1 (MQ=255)                                                 ggTGAGCGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATc                                                                                                                                                                   >  2:589748/1‑251 (MQ=255)                                                  gTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATcc                                                                                                                                                                  <  2:414747/251‑1 (MQ=255)                                                      gagGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACCATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGt                                                                                                                                                              >  1:658285/1‑251 (MQ=255)                                                                 gcCGACTACCATGTCCAATGGTTGAACAATGGTGCAGCCCGGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTGTTGCATATTATTTCGTGCTGGATACGTGTGTATTTAAAGGAAGGGATCAGTAAACCCGCTGGATGGAAGATAGACATTACAAAACGCGAAAAAGCATGGCTGAACGCATGGAAAGATGCAAGAAATTGCCGTCCGCGTTAAAAGAACAg                                                                                                                                                   <  2:475596/251‑1 (MQ=255)                                                                     aCTTCCATGTCCAATGGTTGAACAATGGGGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTGTTGCATATTAGTTCGTGCTGGATACGTGTGGAGTTAAAGGAATGGATCAGTATACCCGCGGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGAAAAGATGCAAAAAATGGCCAGCCGCGTCAAAATAACAGACaa                                                                                                                                               <  2:699663/251‑1 (MQ=255)                                                                          cATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGc                                                                                                                                          >  1:230937/1‑251 (MQ=255)                                                                          cATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATGTCGTTCTGGATACGTGTGGATTTAAAGGAATTGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGATAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGc                                                                                                                                          <  1:659705/251‑1 (MQ=255)                                                                               ccAATGGTTGAACAATGCTGACGCCCTGGCGAGCCCAGTAATCCAGTAAGATCACGATAAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATCCCCGCTGGATGGAAGATATACAGTACAAAAAGGGGCAAAGCAGGGCTTAACGACTGGAAAGATGCAAAAAATGGCAATCCGCGTACacagaacagaaacaatgcccttt                                                                                                                                     >  2:324735/1‑238 (MQ=255)                                                                                 aaTGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTAc                                                                                                                                   <  2:164780/251‑1 (MQ=255)                                                                                 aaTGGGTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTAc                                                                                                                                   <  2:390000/251‑1 (MQ=255)                                                                                     gTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGaa                                                                                                                               <  1:407015/251‑1 (MQ=255)                                                                                         aaCAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGt                                                                                                                            >  1:456279/1‑250 (MQ=255)                                                                                               ggTGCAGTCCTGGCGAGCCCAGTAATCCTGTCAGGACCGGATCAAGCCCTGGAAGGTCCAGGTATCAAACTTTTGCATATTATTTCGTGCTGGATCCGTGCGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGCTATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAACAAATGGCCATCCGCGTCAAAAGAGCAGACAAACTGATCTTTACGGAAGGTATACCcg                                                                                                                     >  2:736226/1‑251 (MQ=255)                                                                                               ggTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGAATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGAATAACcg                                                                                                                     >  1:704125/1‑251 (MQ=255)                                                                                                  gCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCgcgc                                                                                                                  <  2:565774/251‑1 (MQ=255)                                                                                                    aGCCCTGGCGAGCCCAGTAATCCTGTCAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGaa                                                                                                                               >  2:309022/1‑236 (MQ=255)                                                                                                    aGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGaa                                                                                                                               <  1:309022/236‑1 (MQ=255)                                                                                                    aGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCAt                                                                                                                <  1:515171/251‑1 (MQ=255)                                                                                                      cccTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCAttt                                                                                                              <  1:568305/251‑1 (MQ=255)                                                                                                       ccTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATATCCGCGCATTTc                                                                                                             <  1:206397/251‑1 (MQ=255)                                                                                                                 ccAGTAATCCTGTAAGGTCAGGATCAAGCCCTAGAAGGTCCTGGCATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTCTAACCGCGCATTTCATAGCTTccc                                                                                                   >  2:135956/1‑251 (MQ=255)                                                                                                                          cTGTAAGGTCAGGTTCAAGCCCTGGCAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGt                                                                                          >  1:588225/1‑251 (MQ=255)                                                                                                                          cTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGt                                                                                          >  1:96335/1‑251 (MQ=255)                                                                                                                            gTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTc                                                                                        <  1:649964/251‑1 (MQ=255)                                                                                                                              aaGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAATAACAGTCAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCGGGAACTGTTCGa                                                                                      <  2:588225/251‑1 (MQ=255)                                                                                                                                ggTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCgc                                                                                      >  1:509847/1‑248 (MQ=255)                                                                                                                                   cagtaTCAAGCCCTGGAAGGTCCTGGTATCAAACTTGTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATTCAAAAAATGGCCATCAGCGTCAATAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCAGAGCTTCCCTGGAACTGTTCGAAGGc                                                                                  <  1:323214/246‑1 (MQ=255)                                                                                                                                   cAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGGCAAACTGCTCTTTACGGAAGGTAGAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGc                                                                                  <  1:57744/250‑1 (MQ=255)                                                                                                                                          aaGCCCTGGAAGGTCCTGGTATCAAACGTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATTAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACTCATGGAAATATTCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGaa                                                                                     <  1:422568/240‑1 (MQ=255)                                                                                                                                          aaGCCCTGGAAGGTCATGGTATCACACTTTTGCCTATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATCCCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTCCGGAAGGGATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTcaaa                                                                                     >  2:422568/1‑237 (MQ=255)                                                                                                                                                   aaGGTCCTGGTATCAAACTTTTGCATATTATTTCGGGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGAtttttt                                                                 <  2:701333/251‑1 (MQ=255)                                                                                                                                                    aGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATCCGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTACAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTg                                                                >  2:594158/1‑251 (MQ=255)                                                                                                                                                    aGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTg                                                                <  1:332598/251‑1 (MQ=255)                                                                                                                                                    aGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTg                                                                >  1:39340/1‑251 (MQ=255)                                                                                                                                                        ccTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTc                                                             <  1:409406/250‑1 (MQ=255)                                                                                                                                                           ggTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGcc                                                          <  1:367698/250‑1 (MQ=255)                                                                                                                                                                  aacgtttGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTTAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGTGCATTTCATAGCTTCCCGGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTtat                                                  <  1:254824/247‑1 (MQ=255)                                                                                                                                                                    cTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTtata                                                 >  1:591450/1‑250 (MQ=255)                                                                                                                                                                      ttggCATATTATTGCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAAc                                               <  2:235462/247‑1 (MQ=255)                                                                                                                                                                         gCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGtt                                            <  2:256237/250‑1 (MQ=255)                                                                                                                                                                             attattTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTgcc                                       <  1:435011/251‑1 (MQ=255)                                                                                                                                                                                attTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAACAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTgccgcc                                    >  1:355866/1‑251 (MQ=255)                                                                                                                                                                                   tCGTGCTGGATACGTGTGGATTTAAAGGAAGTGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTGAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGACGATTTTTTGCTCGCCGGTGTTATAAc                                               <  1:317932/237‑1 (MQ=255)                                                                                                                                                                                   tCGTGCTGGATACGAGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACACAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGTTTTTTTGCTCGCCGGTGTTATAAccgatcggacgagcg                                 >  2:317932/1‑237 (MQ=255)                                                                                                                                                                                       gCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTGCCCTGGAACTGTTCGAAGTCGGGGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATcc                              <  1:433803/250‑1 (MQ=255)                                                                                                                                                                                           gATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTgaa                         <  2:456279/251‑3 (MQ=255)                                                                                                                                                                                                gtgtGGATTTAAAGGAAGGGATCAGTATCCCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTta                                                   >  1:624544/1‑220 (MQ=255)                                                                                                                                                                                                gtgtGGATTGAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTta                                                   <  2:624544/220‑1 (MQ=255)                                                                                                                                                                                                    ggATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCat                 <  2:509847‑M1/250‑3 (MQ=255)                                                                                                                                                                                                    ggATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATAGACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAGTGTCCATCCGCGTCAAAAGAACAGACAAACTGCGCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatc                <  1:736226‑M1/251‑4 (MQ=255)                                                                                                                                                                                                      aTTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcgg              <  2:573383‑M1/251‑6 (MQ=255)                                                                                                                                                                                                          aaaGGAAGGGAGCAGTATACCCGCGGGATGGAAGAGATAAAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAAAAGACAAACTGCTCTTTACGGAAGGGATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGGGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctc          <  2:391284‑M1/251‑10 (MQ=255)                                                                                                                                                                                                                  ggATCAGTATACCCGCTGGATGGAAGATCTACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGACCAGACAAACTGCTCTTTACGGCAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcac  >  1:282310‑M1/1‑234 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                      AAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAAC  >  NC_000913/3725147‑3725606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑