BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,523,800	C→G	Y310* (TAC→TAG)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,523,800	0	C	G	98.9%	675.2 / NA	180	Y310* (TAC→TAG)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand): ref base C (1/0); major base G (107/70); minor base T (1/1); total (109/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 180 total aligned reads displayed.

GGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGA  >  NC_000913/3523553‑3524046

ggATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGCCACACACTGAGGCGATTAACGCTACCTCTCACGAGCCGGAGATTGATTTCTCTGCGCCGTACCTGAGCGAACTGGTGCGCCTGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGAAGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGGACGTAATAACGTGCTGGACTAGGAc                                                                                                                                                                                                                                                     >  1:700858/1‑251 (MQ=255)

         aTGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCAc                                                                                                                                                                                                                                            <  1:338487/251‑1 (MQ=255)

           gAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACgg                                                                                                                                                                                                                                          >  2:368170/1‑251 (MQ=255)

            aaGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGc                                                                                                                                                                                                                                         >  1:564717/1‑251 (MQ=255)

               ggTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTAt                                                                                                                                                                                                                                      >  2:571229/1‑251 (MQ=255)

               ggTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTGTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTAt                                                                                                                                                                                                                                      <  1:296585/251‑1 (MQ=255)

                  atatCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGCTTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGACCTGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATcgc                                                                                                                                                                                                                                   >  1:696944/1‑251 (MQ=255)

                   tatCACCCAACAACCGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATTGCATTTACACCACCATAACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGCACGGAATCACGTGCTGGACTAGGACATGCGCCACGGCCATcgcg                                                                                                                                                                                                                                  >  2:556144/1‑251 (MQ=255)

                    atCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTTCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCgg                                                                                                                                                                                                                                 <  2:328668/251‑1 (MQ=255)

                    atCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATcgcg                                                                                                                                                                                                                                  <  1:556144/250‑1 (MQ=255)

                        cccAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCg                                                                                                                                                                                                                             >  2:320726/1‑251 (MQ=255)

                           aacaacAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGc                                                                                                                                                                                                                           >  1:386558/1‑250 (MQ=255)

                            acaacaGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCa                                                                                                                                                                                                                          >  2:713064/1‑250 (MQ=255)

                              aacaGTTCGATCAGACACGCACTGAGGCGATTAACGCCAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGCAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAAt                                                                                                                                                                                                                       >  1:684520/1‑251 (MQ=255)

                                 aGTTCGATCAGCCACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCACAtgtg                                                                                                                                                                                                                    >  2:438308/1‑251 (MQ=255)

                                  gTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGc                                                                                                                                                                                                                   <  2:140111/251‑1 (MQ=255)

                                     cGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCtgt                                                                                                                                                                                                                <  2:564717/251‑1 (MQ=255)

                                     cGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGAGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGCGCCTATGAAGACGGTTATCGCATTTACACCCCCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGAGCTGGACTAGGCCACGCGCCACGGCTATCGCGGCCCGGCAAACGTGCtgt                                                                                                                                                                                                                >  1:432590/1‑251 (MQ=255)

                                       aTCAGACATGCACTGAGGCGATTACCGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCtgt                                                                                                                                                                                                                <  2:379183/249‑1 (MQ=255)

                                                gCACTGAGGCGATTACCGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAAAGTGCTGTGGAAAGGGGGc                                                                                                                                                                                                     >  2:488262/1‑251 (MQ=255)

                                                 cACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTAGCGCGGCCCGGCAAATGTGCTGTGGAAAGGGGGCg                                                                                                                                                                                                    <  2:238493/251‑1 (MQ=255)

                                                               aCGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGAt                                                                                                                                                                                      <  2:321605/251‑1 (MQ=255)

                                                               aCGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCACGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGAt                                                                                                                                                                                      <  2:369768/251‑1 (MQ=255)

                                                                cGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGAt                                                                                                                                                                                      >  1:581488/1‑250 (MQ=255)

                                                                 gCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATa                                                                                                                                                                                     >  1:616448/1‑250 (MQ=255)

                                                                  cTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaac                                                                                                                                                                                   >  2:208282/1‑251 (MQ=255)

                                                                  cTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGGGGGCGAGTCGGCGGGGGATaac                                                                                                                                                                                   >  2:176712/1‑251 (MQ=255)

                                                                     aCTATCACGCGCAGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaacaa                                                                                                                                                                                 >  1:35885/1‑250 (MQ=255)

                                                                         tCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAacaaca                                                                                                                                                                               >  1:358636/1‑248 (MQ=255)

                                                                          cACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAacaaca                                                                                                                                                                               >  1:69125/1‑247 (MQ=255)

                                                                             gcgcCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTAc                                                                                                                                                                        >  2:226365/1‑251 (MQ=255)

                                                                              cgcCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTAcc                                                                                                                                                                       >  2:151112/1‑251 (MQ=255)

                                                                               gcCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCg                                                                                                                                                                      >  2:103758/1‑251 (MQ=255)

                                                                                   gagaTTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGCAAGTGGGCGAGTCGGCGGGGGATAACAACAAGATTACCGATAc                                                                                                                                                                  >  2:129608/1‑251 (MQ=255)

                                                                                     gaTTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGc                                                                                                                                                                >  1:423156/1‑251 (MQ=255)

                                                                                     gaTTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGAGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGAGTGGGATAACAACAAGATTACCGATACGc                                                                                                                                                                >  1:149264/1‑251 (MQ=255)

                                                                                       ttGCTTTCTCTGCGCCGTACCCGCGCGAAATGGAGCGCCAGACGCTTCATAACCGTTATGGCGAAAGAGCCTATGAAGACGGTTATAGCATTTACTCTACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGAAGGAGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATAGCGGCCCGGCAAATGGGCCGTGGAAAATGGGAGAGTCGGCGTGGGATAGCAACAAGATTCCCGATACGCTg                                                                                                                                                              >  2:501993/1‑251 (MQ=255)

                                                                                        tGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTg                                                                                                                                                              <  1:57056/250‑1 (MQ=255)

                                                                                             tctcTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCATCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGgc                                                                                                                                                         <  1:159294/250‑1 (MQ=255)

                                                                                                 tGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGc                                                                                                                                                    >  1:624890/1‑251 (MQ=255)

                                                                                                   cgcCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCa                                                                                                                                                  <  1:51446/251‑1 (MQ=255)

                                                                                                    gcCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCa                                                                                                                                                  >  1:456201/1‑250 (MQ=255)

                                                                                                     ccGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAAc                                                                                                                                                <  1:139799/251‑1 (MQ=255)

                                                                                                      cggaCCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGagctatcaa                                                                                                                                                                                                     <  2:396989/194‑9 (MQ=255)

                                                                                                      cGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAAcc                                                                                                                                               >  2:394012/1‑251 (MQ=255)

                                                                                                              aGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATgggcc                                                                                                                                       <  2:427893/251‑4 (MQ=255)

                                                                                                              aGCGAAATGCTGCGCCCGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTcc                                                                                                                                       >  1:596256/1‑251 (MQ=255)

                                                                                                                 gAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCgct                                                                                                                                    <  2:42660/251‑1 (MQ=255)

                                                                                                                   aaTGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACACGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCgctgc                                                                                                                                  >  1:180594/1‑251 (MQ=255)

                                                                                                                       gTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGctgcc                                                                                                                              >  2:381785/1‑251 (MQ=255)

                                                                                                                         gcgcCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGctgcct                                                                                                                             <  1:320726/250‑1 (MQ=255)

                                                                                                                             cAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCACAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGATGCCTGCCGc                                                                                                                        >  2:594020/1‑251 (MQ=255)

                                                                                                                              aGGAGATGTATACCCCTTATGGCGAAAGTGCCAACGCAAACGGTTATCGCATTTACACCACCATCACCCGCACAGTGCCGCAGCCCGCGCAGCAGGCGGTACGAAATAACGTGCCGGACTAGGACATGCGCCACGGCTATAGCGGCCCGGCCAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCGGGAGGCGCTGCCAACCTCTGGTCCGCTGCGGCCTGCCGc                                                                                                                        >  2:78511/1‑250 (MQ=255)

                                                                                                                               ggAGATGTATAACCGTTATGGCGAACGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGc                                                                                                                        >  1:606423/1‑249 (MQ=255)

                                                                                                                                gagaTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGCTTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAgg                                                                                                                     >  2:196752/1‑250 (MQ=255)

                                                                                                                                 agaTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTc                                                                                                                    >  1:435601/1‑251 (MQ=255)

                                                                                                                                 agaTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTAAGGTCCGCTGCTGCCTGCCGCAGTc                                                                                                                    >  2:551634/1‑251 (MQ=255)

                                                                                                                                 agaTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGAGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTc                                                                                                                    >  2:386944/1‑251 (MQ=255)

                                                                                                                                     gTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCAccc                                                                                                                >  2:467039/1‑250 (MQ=255)

                                                                                                                                       ataACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAgc                                                                                                              <  1:537897/251‑1 (MQ=255)

                                                                                                                                         aaCCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCCTCACCCGCAAAGTGCCGAAGGCCGCGCAGCAGGCGGTACGTCATAACGTGCTGGAATAGGACCTGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGCCGGCGTGGGCTAACAACAAGCTTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAgcgc                                                                                                            >  1:291077/1‑251 (MQ=255)

                                                                                                                                          aCCGTTATGGCGACAGTGCCTACGAAGACGGTTATAGCATTTACACCACCATCACCCGCCAAGTGCAGCAGGCCGCGCAGCAGGCGGAACGTAATAACGAGCTGGCCTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCAATGGTCCGCTGCTGCCTGCCGCAGTCCCCAGCGcc                                                                                                           >  2:107535/1‑251 (MQ=255)

                                                                                                                                            cGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTGCCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCaa                                                                                                         <  1:519283/251‑1 (MQ=255)

                                                                                                                                                     gAAAGTGCCGATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTATGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGGCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCa                                                                                                <  2:606423/251‑1 (MQ=255)

                                                                                                                                                      aaaGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCa                                                                                                >  2:222952/1‑250 (MQ=255)

                                                                                                                                                        aGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGc                                                                                             >  1:263691/1‑251 (MQ=255)

                                                                                                                                                         gTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCg                                                                                            <  1:566767/251‑1 (MQ=255)

                                                                                                                                                               aTGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACgg                                                                                        <  1:564218/249‑1 (MQ=255)

                                                                                                                                                               aTGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCg                                                                                      >  1:237221/1‑251 (MQ=255)

                                                                                                                                                                tGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGCTAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCg                                                                                      >  1:427133/1‑250 (MQ=255)

                                                                                                                                                                  aaGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATg                                                                                   >  1:627868/1‑251 (MQ=255)

                                                                                                                                                                       ggTTATCGCATTTACACCACCAGCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCGATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATGCGCTGAAGGCGCTGCCAACCGATGGTCCTCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGTCGGCGATGCTGGc                                                                              <  2:696944/251‑1 (MQ=255)

                                                                                                                                                                        gTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACAAGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCg                                                                             >  1:393986/1‑251 (MQ=255)

                                                                                                                                                                          tATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCgg                                                                            >  1:214928/1‑250 (MQ=255)

                                                                                                                                                                            tCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACg                                                                         >  1:713403/1‑251 (MQ=255)

                                                                                                                                                                                aTTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTc                                                                     <  1:488766/251‑1 (MQ=255)

                                                                                                                                                                                 tttACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGCCGGCGATGCTGGCGGAccgggcg                                                                    >  2:27138/1‑245 (MQ=255)

                                                                                                                                                                                   tACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGAc                                                                  >  1:48558/1‑251 (MQ=255)

                                                                                                                                                                                    acacCACCATCACCCGCAAAGTGCGGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGGCTAGGACATGCGCCACGGCTATCGCGGCGCGGCAAATGTGCTGGGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATGACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCGTCTGCGTGCCGCAGTCACCAGCGCCAAGCCTCAGCAAGCGACGGCGATGATGGCGGACGGGTCGAc                                                                  <  2:344812/250‑1 (MQ=255)

                                                                                                                                                                                    acacCACCATCACCCGCAAAGTGCGGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTAGCGCGACCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGTAGGCGCTGCCAACCTGTGGTCCGCTGCTGCCGGCCGCAGTCGCCAGCTCCAATCCACAGCAAGCGACGGCGATGCTGGCGGACGGGTCGAcc                                                                 <  2:675239/251‑1 (MQ=255)

                                                                                                                                                                                      accaccATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGAAATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGAGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGCAGGCGCGGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGt                                                               >  1:54471/1‑251 (MQ=255)

                                                                                                                                                                                        caccaTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGCACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACCAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGCTGCTGGCGGACGGGTCGACCggcg                                                             >  2:547254/1‑248 (MQ=255)

                                                                                                                                                                                         accaTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGCACGTAATAAAGTGCTGGACTCGGACATGCGCCACGGCTATCGCGGCCGGGCAAATGTGCTGTGGACAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGc                                                            >  1:348072/1‑251 (MQ=255)

                                                                                                                                                                                            aTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCAtt                                                         >  2:491155/1‑251 (MQ=255)

                                                                                                                                                                                              cACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCCTTgg                                                       >  2:84893/1‑250 (MQ=255)

                                                                                                                                                                                               aCCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCCAGCGACGGCGATGCTGGCGGACggggccacaggcgcattggg                                                      >  2:120865/1‑234 (MQ=255)

                                                                                                                                                                                                cccGCAAAGTTCAGCAGGCCGCGCGGCAGGCGGGACGTAATAACGTGCTGGACTAGGACATGCGTCACGGCGATCGTGGCCCGGCAAATGTGCTGTGGAAGGTTGGCGAGTCGGCGTGGGATAACAACAAGAATACCGATACGCTGAAGGCGCTGCCAACCTGTGGGCCGCTGCGGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGAATTGAGt                                                     <  1:459529/251‑1 (MQ=255)

                                                                                                                                                                                                   gCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACATGCTGGACTCGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGATGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTAACGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGGATg                                                  >  1:423405/1‑251 (MQ=255)

                                                                                                                                                                                                      aaGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGCCTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGGCGGCGTGGGATAACACCAAGATTACCGATACGCTGACGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGCCGACCGTCGcctttggtatggg                                                >  2:19063/1‑238 (MQ=255)

                                                                                                                                                                                                        gTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAgg                                             >  2:258053/1‑251 (MQ=255)

                                                                                                                                                                                                         tGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGGCGACCGTCGCATTGAGTCTGGAAGGc                                            >  2:65741/1‑251 (MQ=255)

                                                                                                                                                                                                           cagcagGCCGCGCAGCAGTCGGTACGTAAGAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCGGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGt                                          <  2:619619/251‑1 (MQ=255)

                                                                                                                                                                                                              cagGCTGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCg                                       >  1:226838/1‑251 (MQ=255)

                                                                                                                                                                                                              cagGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCg                                       <  1:379524/251‑1 (MQ=255)

                                                                                                                                                                                                                        agcagGCGGTACGTAATACAGTGCTGGACTATGACATGCGCCCCGGCTATCGCGGCCCGGCAAATGTGCTGAGGAAAGTGGGCGCGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGCCGCGTCGCACGTCGCCTTGGGTATgggaagagttcgccggtcgagc                             >  2:467082/1‑231 (MQ=255)

                                                                                                                                                                                                                                           cGTGCTGGACTAGGACATGCGCCACGGCTGTCGCGGCCCGGCAAATGTGCTGTGGAAAGGGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTc          <  2:358636/251‑1 (MQ=255)

                                                                                                                                                                                                                                           cGTGCTGGACTAGGACAGTCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTc          <  2:596256/251‑1 (MQ=255)

                                                                                                                                                                                                                                                  gACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGACAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAgg   >  1:721281/1‑251 (MQ=255)

                                                                                                                                                                                                                                                  gACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTggg                                  >  1:210694/1‑220 (MQ=255)

                                                                                                                                                                                                                                                   aCTAGGACATGCGCCACGGCTAGCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGGGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGa  <  1:196752/251‑1 (MQ=255)

GGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGGA  >  NC_000913/3523553‑3524046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`