Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,756,045	T→C	intergenic (+90/‑114)	ssrA → / → intA	tmRNA/integrase IntA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,756,045	0	T	C	98.2%	181.5 / ‑4.0	57	intergenic (+90/‑114)	ssrA/intA	tmRNA/integrase IntA
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (28/27);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.68e-01

CCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTATGACGGTGACGGGCTTACTCTGTTAATCAAGTCCAGTGGC  >  NC_000913/2755802‑2756275                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       cctgcctgGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTccc                                                                                                                                                                                                                                 <  1:566190/251‑1 (MQ=255)    gcctgGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTATTACCGAGGATGTAGGAATTGCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCcg                                                                                                                                                                                                                                <  1:290317/249‑1 (MQ=255)           gtagcaGCGTTAAAACTGAATCGGGCTAGTTTGTTAGTGGCGAGTCCGTCAGCAGCTGGCAAGCGAATGTAAAGACTAACGAAGCATGTAGTACCGAGAATGAAGAAATTCCGGACGCGGGTACAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCg                                                                                                                                                                                                                       <  2:15702/246‑1 (MQ=255)            ttGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGGTCGGAGCTGGCAAAGGAATGTAAAAAATGACTAAGCATGTAGTAACGAGGGAGTAGGAAATTTGGGCGCGGGGTCAACTCCCGCCCGCTCCACCAAAATTCTCCATCGGGGATTACCAGCGTCAACCGCAGAAGTCCTAAGAGCACGCACGGCGAAAACCCCGGCGGCCTTTTTGTGCCCTCACATCCTCCCGCGAAGTccc                                                                                                                                                                                                                       >  2:524345/1‑249 (MQ=255)                aGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAagag                                                                                                                                                                                                                  <  1:694976/251‑1 (MQ=255)                      aaaaCTTAATCAGGCTAGTTTGTTAGTGGCGGGCCCGTCCGCAGCTGGCAAGCGAAGGTAAAGACTGACTAAGCATGTAGTACCGAGGAGGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAAGTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTa                                                                                                                                                                                                             <  1:686981/250‑1 (MQ=255)                             aaTCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAAt                                                                                                                                                                                                      <  1:525899/250‑1 (MQ=255)                                                gggggtgcccgtccgCAGCTGGCAAGGGAATGTAAAGACTGACTAAGCATGTAGGACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCAtt                                                                                                                                                                                  <  2:541720/243‑1 (MQ=255)                                                 ggCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTg                                                                                                                                                                                 <  1:675711/251‑1 (MQ=255)                                                  gCGTGTCCGTCCGCAGCTGGCAAGCGCATGAAAAGACTGACTAAGCATGTAGTAACGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTg                                                                                                                                                                                 >  2:692160/1‑250 (MQ=255)                                                         cgtccgCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCCTGTCGTACCGAGGATGTAGGCATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTACGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGccc                                                                                                                                                                         >  1:474301/1‑251 (MQ=255)                                                         cgtccgCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGCAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGccc                                                                                                                                                                         >  2:506731/1‑251 (MQ=255)                                                         cgtccgCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGccc                                                                                                                                                                         >  2:11191/1‑251 (MQ=255)                                                           tccgCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGcc                                                                                                                                                                          >  2:194098/1‑248 (MQ=255)                                                           tccgCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGcc                                                                                                                                                                          <  1:194098/248‑1 (MQ=255)                                                                aGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGCATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGaa                                                                                                                                                                   >  1:129474/1‑250 (MQ=255)                                                                aGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGaa                                                                                                                                                                   >  1:254655/1‑250 (MQ=255)                                                                 gCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGGACAGAGGATGAAGGAATTTCGGACGCGGGTTCAACACCCGCCAGCTCCCCCCAAATTCTCCATCGGCGATTACCAGACTCATCCGATGAAGTCCTAAGAGCACGCACGGCGCAAGCCATGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGACGCGACCTAATTAAATCCGAAACTTTTCGGCCCATTGATAGGCACAACGaca                                                                                                                                                                  >  1:707690/1‑248 (MQ=255)                                                                    ggCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGTCCAACGAAAAGct                                                                                                                                                              <  1:574026/251‑1 (MQ=255)                                                                        aGCGAATGTAAAGACTGACTAAGCATGTAGTATCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCCGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTTGGCCCCTTGATATGGCCAACGAAAAGATCTAt                                                                                                                                                          >  2:256992/1‑251 (MQ=255)                                                                          cGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTg                                                                                                                                                        >  1:431782/1‑251 (MQ=255)                                                                               gTAAAGACTGTCTAAGCATGTAGTTCCGCGGATGTAGGAATTGCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCGCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTAc                                                                                                                                                   <  1:469048/251‑1 (MQ=255)                                                                                        gactAAGCATGTCGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGcc                                                                                                                                           <  1:503563/250‑1 (MQ=255)                                                                                             agcagttagtaCCAAGGATGTAGGAATTCGGGAGGGGGGTTAAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACg                                                                                                                                     <  2:145610/245‑1 (MQ=255)                                                                                                    agtaCCGAGGATGTAGGAATTCCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCAGCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGga                                                                                                                               <  1:553624/250‑1 (MQ=255)                                                                                                       aCCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACt                                                                                                                           >  2:275150/1‑251 (MQ=255)                                                                                                        ccGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCCGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTc                                                                                                                          >  2:484361/1‑251 (MQ=255)                                                                                                                gTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGACCTAAGACCCCGCACGGCGCAAGCCCTGAGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGACGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGc                                                                                                                  >  1:307858/1‑251 (MQ=255)                                                                                                                               gcgGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGcc                                                                                                   >  1:416303/1‑251 (MQ=255)                                                                                                                               gcgGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGcc                                                                                                   >  1:344026/1‑251 (MQ=255)                                                                                                                                          tCCCGACAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGCAGACCGAAGAGAACTAATTAAATCAGACCCTTTTCGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGCCCTCACCGCAGGGAGACGCCCCATTGCCAGAAAAACCACGCCGTTaccttat                                                                                         >  2:673487/1‑244 (MQ=255)                                                                                                                                           cccGCCAGCTCCCCCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGCCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACTTTGGGCCTAAACGCAGGGATACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGAt                                                                                         <  1:482095/249‑1 (MQ=255)                                                                                                                                                   cTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTTGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATc                                                                                >  2:238095/1‑250 (MQ=255)                                                                                                                                                    cccaccaAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGGGGGCTTTTTTGTGCCCTCAACCCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGGTAACTGATACGGAAATCaa                                                                              <  2:431782/250‑1 (MQ=255)                                                                                                                                                          aaaaTTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTATTGAAATCCGACCCTTTAAGGCCCATTGAGAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCGGGGAGACTCCCCATGCCAAGAAAAGCCAAGCCATTAACTGATACGGAAATCAAAgccgc                                                                        <  2:71155/251‑1 (MQ=255)                                                                                                                                                                  ccatcggggatgcccagatacagccGATGAAGTCCTAAGAACCCGCACCGCGCAAGCCCGGCGGGCTTTTTTGTGCCCTCAACGCGTCCCGCGAAGTCCGAAGAGAACTAATTAAACCCGATCCTTTAAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCAGGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAAcc                                                                  <  2:129474/226‑1 (MQ=255)                                                                                                                                                                    aTCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACCCAGGGACACGCCCCATGGCAAGAAAAACCCAGCCGTTAACTGATCCGGAATACCCAGCCGCCACACCTaa                                                               >  2:23757/1‑250 (MQ=255)                                                                                                                                                                     ccGGTGATTCCCAGATTTATCCGATGAAGTCCTAAGAGCCCGCACGGCAAAATCCCTTCGGGCTTTTTTGTGCCCACAACGCGTCCCGCAAAGTCCGAAGAGAACTAATAAAAGCCAACCCTTTTAGACCCATTTAAAGCCCCAAGGAAAACCTCTTTTGTTTACTTTGGGCATAAACGCAGGGAGACTCCCCATGCCAAGAAAAACCAAGCCGTTAACTTATACGGAAATCAAATCCGCCAAACCTAAAg                                                             <  2:651504/250‑1 (MQ=255)                                                                                                                                                                        gTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCCCGCCATGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTCATTAAATCCGACCCTTTTAGGCCCATTGACAGGCCCAACGACACGCTCTATTGTTTACGTTGGCACTAAACGCAGGGCGACTCACCATGGCAAGAAACAACAAGCCGTTAACTGATACGGACATCAAAGCCGCCAAACCTAAAGATg                                                          >  1:729100/1‑251 (MQ=255)                                                                                                                                                                           atttccAGAGGCACCCGATGAAGTCCTAAGAGCCCGCCGGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGCCCCGGGAAGTCGGAAGAGAACGAATTAAACCCGAACTTTTTGGGCCCATTGAGAGGCCCAACGAAGAGCGCGATTGTTTCCGTTGGGCCTAAGCGCAGGCATACGCCCCATGGCAAGAAAAACTGAGCGGTTAACTGATTCGGAAGTTAAAGCCGGCAAACCTAAAGATGCCg                                                       <  2:377330/247‑1 (MQ=255)                                                                                                                                                                             tACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGAt                                                     >  1:605711/1‑251 (MQ=255)                                                                                                                                                                               ccAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGCAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGAtt                                                    >  2:438575/1‑250 (MQ=255)                                                                                                                                                                                cAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTAc                                                  <  1:256992/251‑1 (MQ=255)                                                                                                                                                                                 agagccttcctaTGAAGTCCTAAGAGCCCGCACGGCGCAGGCCCTGCGGGCTTTGTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAACCCGCACCTTTTAGGCCCATTGAAAGGCCCAACGAAAACCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAACCCAAGCCGGTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATTCCGATTAcc                                                 <  2:425125/240‑1 (MQ=255)                                                                                                                                                                                    gTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCGCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGc                                              >  2:590433/1‑251 (MQ=255)                                                                                                                                                                                      cATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCtt                                            >  2:159745/1‑251 (MQ=255)                                                                                                                                                                                            aTGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTAtgac                                      >  1:491578/1‑251 (MQ=255)                                                                                                                                                                                            aTGAAGTCCTAAGAGCCCGCACGGCCCAAGCCCTGCGGGCTTTTTTGGGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCGATTGTTTAGGTTGGGCATAAACGCAGGGAGACTCCCGATGGCAAGAAAAACCAAGCCGTTAACTGATACGTGAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTAtgac                                      <  1:293534/251‑1 (MQ=255)                                                                                                                                                                                              gAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTAtgacgg                                    <  1:560415/251‑1 (MQ=255)                                                                                                                                                                                                aGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTAtgacggtg                                  <  1:159745/251‑1 (MQ=255)                                                                                                                                                                                                    cTAAGAGCCAGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGCTAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTAtgacggtgacgg                              >  2:365787/1‑251 (MQ=255)                                                                                                                                                                                                       agagCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGACCCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTTGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTATGACGGTGACGGGCt                           <  1:238095/251‑1 (MQ=255)                                                                                                                                                                                                              gCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTATGACGGTGACGGGCTTACTCTg                    >  1:42834/1‑251 (MQ=255)                                                                                                                                                                                                               cccGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTTTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTATGACGGTGACGGGCTTACTCTGt                   <  2:42834/249‑1 (MQ=255)                                                                                                                                                                                                                   gcgcAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTATGACGGTGACGGGCTTACTCTGTTAAt               >  2:159622/1‑251 (MQ=255)                                                                                                                                                                                                                    cgcAAGCCCTGCGGGCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAg                                                                                                               <  2:246886/154‑1 (MQ=255)                                                                                                                                                                                                                                  gCTTTTTTGTGCCCTCAACTCGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTATGACGGTGACGGGCTTACTCTGTTAATCAAGTCCAGTGGc  >  2:43863/1‑249 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                       CCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGTAAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGTCCTAAGAGCCCGCACGGCGCAAGCCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTATGACGGTGACGGGCTTACTCTGTTAATCAAGTCCAGTGGC  >  NC_000913/2755802‑2756275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑