Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,242,968	G→T	intergenic (‑1/+16)	yeiB ← / ← folE	DUF418 domain‑containing protein YeiB/GTP cyclohydrolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,242,968	0	G	T	100.0%	198.4 / NA	52	intergenic (‑1/+16)	yeiB/folE	DUF418 domain‑containing protein YeiB/GTP cyclohydrolase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/23);  total (29/23)

GTCTGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCT  >  NC_000913/2242724‑2243213                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      aacctggcaccacgtaaaatgcgcttcggtatctctgacggcatggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttttaaattcctaaagttgttttctttcgattttgtctctctctaacccccataaACGAGCGTGACGGTGCGCTCCATTGTTCCt                                                                                                                                                                                                                                                 >  2:593944‑M2/222‑251 (MQ=255)   cctggcaccacgtaaaatgcgcttcggtatctctgaaggcatggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttgtcttgcgattttgtctctctctatcccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGc                                                                                                                                                                                                                                               <  2:726252‑M2/32‑1 (MQ=255)       gcaccacgtaaaatgcgcttcggtatctctgaaggcatggtgatggctgccggtcctggcggggaagatattttcctgctaagtccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcatcaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCttt                                                                                                                                                                                                                                           >  2:168320‑M2/216‑251 (MQ=255)              gtaaaatgcgcttcggtatctctgaaggcatggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatggcggtgctaaaacgggtcatcaggggcactaatcccccttcaaagcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGGTACGTTGCGCTCCCTTGTTCCCGCCTTTTAatccg                                                                                                                                                                                                                                    >  2:635821‑M2/209‑249 (MQ=255)                                      aaggcatggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGa                                                                                                                                                                                                            <  1:143199‑M2/67‑1 (MQ=255)                                           atggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccggggcatcaggtgaaataatcccccttaaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgcttttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTc                                                                                                                                                                                                       >  1:301758‑M2/180‑251 (MQ=255)                                                 atggctgccggtcctggcggtaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg                                                                                                                                                                                                 <  1:2729‑M2/78‑1 (MQ=255)                                                 atggctgccggtcctggcgggaaagatatttccctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatccccctgcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgcg                                                                                                                                                                                                 <  2:392813‑M2/78‑1 (MQ=255)                                                                 gcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcagtggaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGAt                                                                                                                                                                                 <  2:301758‑M2/94‑1 (MQ=255)                                                                      aaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGACACTCGTGGCGCGTATTCTGACTGGATTTGa                                                                                                                                                                             >  2:156365‑M2/153‑250 (MQ=255)                                                                      aaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGa                                                                                                                                                                             >  2:166533‑M2/153‑250 (MQ=255)                                                                                        agcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagagccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGaggg                                                                                                                                                          >  2:432171‑M2/135‑250 (MQ=255)                                                                                         gcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTc                                                                                                                                                         >  1:231800‑M2/134‑251 (MQ=255)                                                                                            cggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACGCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGtt                                                                                                                                                      <  1:648634‑M2/121‑1 (MQ=255)                                                                                            cggatgccggtgctaaaccgggtcatcaggtgaaataatccccctgcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGtt                                                                                                                                                      <  2:231800‑M2/121‑1 (MQ=255)                                                                                             ggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCACGAGAGGTCGTTg                                                                                                                                                     >  2:578214‑M2/130‑251 (MQ=255)                                                                                              gatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTGGAACAATCCACCAAGAGAGGTCGTTGt                                                                                                                                                    <  1:544365‑M2/123‑1 (MQ=255)                                                                                                           aaccgggtcatcaggtgaaataatcccccttcaaggcgctgcaaagacaacgccttttctttataaatttctaaagttgttttattgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATAAGTTGTGATGACGCACAGCGCGCAGAAAATCGTGGCGCGTATTCTGACTGGATTTGCACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGtt                                                                                                                                       >  1:385567‑M2/116‑251 (MQ=255)                                                                                                                 gtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATcg                                                                                                                                 <  2:273156‑M2/142‑1 (MQ=255)                                                                                                                   catcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATcgc                                                                                                                                <  2:134267‑M2/143‑1 (MQ=255)                                                                                                                            aaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCAcg                                                                                                                      <  1:156365‑M2/153‑1 (MQ=255)                                                                                                                                             ggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAAt                                                                                                     <  1:230759‑M2/170‑1 (MQ=255)                                                                                                                                                   gcaacgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACcg                                                                                               <  2:495686‑M2/176‑1 (MQ=255)                                                                                                                                                     atcgaccgcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCAcg                                                                                                                      >  2:312782‑M2/74‑226 (MQ=255)                                                                                                                                                     atcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCGTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCAcg                                                                                                                      <  1:312782‑M2/153‑1 (MQ=255)                                                                                                                                                       cgacatcgccttttctttataaattactaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGCGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGtc                                                                                           >  1:421367‑M2/72‑251 (MQ=255)                                                                                                                                                        gacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGtcg                                                                                          >  1:418997‑M2/71‑251 (MQ=255)                                                                                                                                                         acagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGtcg                                                                                          >  1:162020‑M2/70‑250 (MQ=255)                                                                                                                                                          cagcgccttttctttataaattcctaaagttgttgtcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTGTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGGAATGCACCGCGtcgat                                                                                        <  2:361064‑M2/183‑1 (MQ=255)                                                                                                                                                           agcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGtcgatc                                                                                       <  1:170394‑M2/184‑1 (MQ=255)                                                                                                                                                            gcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGtcgatcg                                                                                      <  1:134658‑M2/185‑1 (MQ=255)                                                                                                                                                                cttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGAc                                                                                  >  1:78942‑M2/63‑251 (MQ=255)                                                                                                                                                                            aattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTAtt                                                                      >  1:107263‑M2/51‑251 (MQ=255)                                                                                                                                                                            aattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAAACAGCCACGTTAtt                                                                      >  1:566503‑M2/51‑251 (MQ=255)                                                                                                                                                                            aattcctaaagttgttttattgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGGGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTAtt                                                                      >  1:210079‑M2/51‑251 (MQ=255)                                                                                                                                                                             attcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTg                                                                     >  2:248776‑M2/50‑251 (MQ=255)                                                                                                                                                                                          ttttcttgcgattttgtatctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGAACTGGTTGCATCGCGGATGCCACGGGCCTTCACGCAGTAATGCACCGCGCCGATCGAGACAGCCACGTTATTGGTGCCAACCAGCg                                                        >  1:600891‑M2/37‑251 (MQ=255)                                                                                                                                                                                               ttgcgattttgtctctctataacccgcacaaTCGAGCGTAACGTTGCGCTCCCTTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATAGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCACCGTTATTGGTGCCCAGCAGCGTTTGt                                                   >  2:86920‑M2/32‑251 (MQ=255)                                                                                                                                                                                                                    acccgcatcaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGAGCAGAAACTCGTGGCGCGTATTATGACTGGATTTGAACAATCCACCCAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGCTGCCACGCGCCTTCACGCAGTAATGCCACGCGTCGCACGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTCGCGCCATAAGCATttgctgt                              >  2:637694‑M2/11‑250 (MQ=255)                                                                                                                                                                                                                     cccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCGGTTGTGATGGCGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACACTCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAAtttgctgtg                             >  2:128206‑M2/10‑249 (MQ=255)                                                                                                                                                                                                                       cgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTc                           <  1:166533‑M2/244‑1 (MQ=255)                                                                                                                                                                                                                        gcataaTCGAGCGTGACGTTGCGCTCAATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGCCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAatttgctgtgt                            >  1:631826‑M2/7‑246 (MQ=255)                                                                                                                                                                                                                        gcataaGCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCGTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGGTGCATCGCGGATGCCACGCGCCTGCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAatttgctgtgt                            <  2:631826‑M2/243‑4 (MQ=255)                                                                                                                                                                                                                          ataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAGGaatttgctgtgcc                           >  2:554997‑M2/5‑244 (MQ=255)                                                                                                                                                                                                                            aaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACg                      >  1:280732/1‑251 (MQ=255)                                                                                                                                                                                                                             aTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCTCAGCGCGCAGAACCTCGTGGCGCGCATTCTGACTGGATTTGAACACTCCCCCAAGCGAGGTCGTTGTCGTGGCCCTGGTTGCATCGCGGATGCTCCGCGCCTTCACGCAGTAATGCACCGCGTCGTTCGAGGCAGCCACGTTATTGGTGCCCAGCCGCGTTTGTCGCGCAATAAGACTTTGCTGTGTCAGatgt                     >  2:356492/1‑248 (MQ=255)                                                                                                                                                                                                                               cGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGtt                    <  1:637411/250‑1 (MQ=255)                                                                                                                                                                                                                                gAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCTTATTCTGACTGGATTTGAACAATCCACCAAGAGAGTTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGGGACAGCCACGTTATTTGTGCCCAGCAGCGGTTGTAGCGCAATAAGAAGTGGCTGTGTCATACGTTcc                  <  2:385567/251‑1 (MQ=255)                                                                                                                                                                                                                                      gACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCAcc            >  1:358945/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGCCGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGc         >  1:70919/1‑251 (MQ=255)                                                                                                                                                                                                                                             cgcTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGAc     <  2:70919/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ccATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGCGGACGCt  <  1:593944/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GTCTGCCACGGGGCAGCAACATTTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGGACGCT  >  NC_000913/2242724‑2243213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑