Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,605	0	T	C	35.2%	101.5 / 14.9	55	G187G (GGA→GGG)	ycfT	inner membrane protein YcfT
Reads supporting (aligned to +/- strand):  ref base T (32/3);  new base C (0/19);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 8.39e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTC  >  NC_000913/1174356‑1174842                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   cagcagGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATACCACTGTTCCTTc                                                                                                                                                                                                                                                   >  2:8994/1‑251 (MQ=255)  agcagGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAACTTGCGG‑ATCACACTGTTCATTcc                                                                                                                                                                                                                                                  >  2:713025/1‑251 (MQ=255)    cagGCGGGTGGAGCGCATACCGAATCGTTGCTCGTATGGATAAAACAGCTTCAGGATCACCCCAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑AGCACACGGTTCATTcccc                                                                                                                                                                                                                                                <  2:122959/251‑1 (MQ=255)       gCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCATATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATGCCccacc                                                                                                                                                                                                                                             <  2:498929/251‑1 (MQ=255)       gCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAAGCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCccacc                                                                                                                                                                                                                                             <  2:41527/251‑1 (MQ=255)                                       aTTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTc                                                                                                                                                                                                          >  2:256579/1‑251 (MQ=255)                                                   aagccgtgtgctctcccaatctccccAGCGACAACAGCAGCGAGATAGAAAACAACCGGCCCCCGCCCGCCAGAAGGGTAAGCAAAAAACCC‑ATCAGCAGAGGGCGGCGCAACGGCCCCTCTTTAACACGGGTCATTATTGTTGCGCAAAACCATGCGCCAGGGCTTAAAAAAGGCAAATTGCGG‑ATCACACTGTT‑ATTCCCCCCCCCCGGCG‑TGGGAA‑CAAAATTAACCGCCACACTCAGCAGTacaaac                                                                                                                                                                                              <  1:69769/227‑1 (MQ=255)                                                     ttCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACCACCAGGCACCGACCGCCAGAACGGTAAGCAAAGACCCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTacaaaca                                                                                                                                                                                             >  1:82291/1‑250 (MQ=255)                                                             caccacAATCGCTACCAGCGACAACAGCAGCGCGATAGTACACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGACGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCCAGGCTGTAATAAGGCACATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAAt                                                                                                                                                                                     >  2:455047/1‑250 (MQ=255)                                                              accacAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAAt                                                                                                                                                                                     >  1:340916/1‑249 (MQ=255)                                                                cacaATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTg                                                                                                                                                                                 >  1:30076/1‑251 (MQ=255)                                                                 acaATCGATACCAGCGACAACCGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCCGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAgggg                                                                                                                                                                                >  2:366938/1‑248 (MQ=255)                                                                  caATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGc                                                                                                                                                                               >  1:16123/1‑251 (MQ=255)                                                                   aaTCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGc                                                                                                                                                                               >  2:550735/1‑250 (MQ=255)                                                                      cGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGTGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAgcg                                                                                                                                                                           >  1:378003/1‑251 (MQ=255)                                                                         tACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTacaaac                                                                                                                                                                                              >  2:69769/1‑229 (MQ=255)                                                                            cAGCAACAACAGCAGGGAAAAAGAAAACACCCAGGCCCCGCCCCCCAGAACGGAAAACAAAGAAGCC‑AACAGCAGAGGGGGGCCCAACGGCCCCTCTTAAACAAGGGTCATTATTGTGGCGCAAACCCACGCGCAAAGGTTGAAATAAGGAAAATGGCGG‑ACCCCACTTTTCTTCCCCCCCCAC‑GGGG‑GGGAAA‑AGAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGAc                                                                                                                                                                     <  2:560973/251‑1 (MQ=255)                                                                               cGACAACAGCAGCGAGATCGTAACCAACCAGGCACCGACCGCCAGAACGGCAAGCAAAGAAGCC‑ATCAGCAGCTGGCGGCGCAACGGCACCTCTTTAACACAGGTCCTTATTGTTGCGCCACACCATGCGCCAAGGCTGTCATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACACAGCAGTACAAACAAGGCGAATAGGGGCAGCGCCAGACGGc                                                                                                                                                                  >  2:127723/1‑251 (MQ=255)                                                                                   aacaGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaa                                                                                                                                                               >  1:80879/1‑250 (MQ=255)                                                                                          gcGAGATAGTAAACAACCAGGCACCGACCGCCAGACCGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCCACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCACGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAACCAAGGCGAATAGTGGCAGGGCCCGACGGCTAAAAAtttt                                                                                                                                                        >  2:304030/1‑250 (MQ=255)                                                                                            gagaTAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTAc                                                                                                                                                      >  1:548001/1‑250 (MQ=255)                                                                                                aaGTAAACAACCAGGCACCCCCCGCCAGAACGGTAAGAAAAGAGCCC‑ATCAGCAGATGGCGGCGCACCGCCACCTCTTAAACACAGGTCATTATTGTTGCGCCAAACCAGGCGCCAGGGCTGAAAAAAGGCAAATGGCGG‑ACCACACTGTTCATCCCCCCCCGC‑GGCG‑TGGAAA‑CAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATAcc                                                                                                                                                 <  2:80879/250‑1 (MQ=255)                                                                                                     aacaacCAGGCACCGCCCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCaaga                                                                                                                                             >  2:112670/1‑247 (MQ=255)                                                                                                                      cgccgaaaCGGTAAGCAAA‑AAGCCCAGCAGCAGAGGGCGGCCCAACGGCTCCTCTTTAACACTGGTTATTTTTGTTGCGCCAAACCAGGCGCCAAGGCTTAAATAAGGAAAATTGCAG‑AACACCCTGTTCCTCCCCCGCCA‑‑GGCGGTGAGAA‑GAAAATCAACCGCCACTCTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCATACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCAt                                                                                                                           <  1:69703/245‑1 (MQ=255)                                                                                                                        ccAGAAGGGAAAGAAAAGAAACC‑AACAGCAGGTGGGGGCGCAACGGCACCTCTTAAACACAGGTAATTTTTGTGGCCCAAAACCATGCGCCAGGGCTGTAAAAAGAAAAATTGCGG‑ATCACCCTGTTCATCCCCCCCCAC‑GGCG‑GGGGAA‑CAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATAc                                                                                                                         <  1:8994/251‑1 (MQ=255)                                                                                                                           aaaagggaaaaaaaaaaagcaagcagcagATGGGGGCCAAACGCCCCCTTTTAAACACGGTTCATTATTGTGGCCCAAAACCAGGCGCCAAGGTTGTAAAAAGG‑AAAATGCGGGATCACACTGTTCTGCCCCCCCCAC‑GGC‑‑TGGGAAACGAATTAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAg                                                                                                                       <  1:612799/228‑1 (MQ=255)                                                                                                                                 aaaGCAAAAAAGCC‑ACCGGCAGGTGGCGGCGCACCGGCACCTCTTTAAAACAGGTCATTATTGTGGCCCCAAACCATGCGCCAGGGCGTTAAAAAGGCAAATGGCGA‑ATCACTCTGTTAATCCCCCCCCCC‑GGCG‑TGGAAA‑CGAAATTTACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTGACATACCACGAAATAGACAATTAACGCATACAGATACcac                                                                                                                <  1:455047/250‑1 (MQ=255)                                                                                                                                       aagaagCC‑AGAAGAAGAGGGGGGCGCAACGGCACCTCTATAACACAGATAATTTTTGTTGCCCCAACCCGTGCGCAAAGGCTGAAAAAAGACAAATGGCGG‑ATCACCTTTTTCATCCCCCCCCAG‑GGCG‑GGGGAA‑CAAAATTATCCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTg                                                                                                          <  2:251056/251‑1 (MQ=255)                                                                                                                                                   agcagATGGGGGAGGAGCGGCACCTCTTAAACACAGGTAATTTTGGTTCCGCAAAACCAGGCGCCAAGGCTGTAAAAAGGCAAATGGCGG‑ATCACACTGTTCATCCCCCACCAC‑GGGG‑GGGAAA‑CGAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGt                                                                                               <  2:548001/251‑1 (MQ=255)                                                                                                                                                   acgagATGGGGGCGCAACGGCCCCTTTTAAACACAGTTATTTATTGTTGCGCAAAACCATGCGCCAAGGCTGAAAAAAGGAAAATTGCGG‑ATCCCACTTTTCATCCCCCCCCAC‑GGCG‑TGGGAA‑GAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACGTACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGt                                                                                               <  1:698648/248‑1 (MQ=255)                                                                                                                                                       gagggcggcGCACCGGCCCCTCTTAAACAAAGGTCATTATTGTTGCGCAAACCCATGCCCAAAGGCTGTAAAAAGGAAAATGGCGG‑ATCCCCCTTTTCATCCCCCCCCAC‑GGCG‑TGGAAA‑CAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑atc                                                                                          <  1:256579/248‑1 (MQ=255)                                                                                                                                                          ggcggcGCACCGCCCCCTCTTTAACACGGTTCATTATTGTGGCGCAAACCCATGCGCCAAGGCTGTAAAAGGGCAAATGGCGG‑ATCACACTGTTCATCCCCCCCCAC‑GGCG‑GGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑atcatc                                                                                       <  1:184559/251‑1 (MQ=255)                                                                                                                                                                  aaCGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGc                                                                                 >  1:484256/1‑249 (MQ=255)                                                                                                                                                                               tAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTACTAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑AACATCCCGTGCCGGAAC‑TCACCgggg                                                                 >  1:272980/1‑249 (MQ=255)                                                                                                                                                                               tAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGATGTCATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCCGCGCCAGACGGCTAAAAATTTTACATACCACGACATAGCCAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGAG‑ATCATCCCGTGCAGGAAC‑TCACCGGTg                                                                 >  1:491638/1‑251 (MQ=255)                                                                                                                                                                                 acacAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAC‑CGAACTTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGTggg                                                               >  1:339201/1‑250 (MQ=255)                                                                                                                                                                                 acacAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATcccctgacggaacccacccggggg                                                                >  2:93041/1‑230 (MQ=255)                                                                                                                                                                                  cacaGTTCATTATTGTTGCGCAAACCCAGGCCCCAAGGCTGAAAAAGGGCAAATTGCGG‑ATCACACTTTTCTTCCCCCCCCGC‑GGCG‑TGGAAA‑CAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑Tc                                                                        <  1:147707/241‑1 (MQ=255)                                                                                                                                                                                  cacaGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑GTCATTCCGTGGAGGAAC‑TCaaccgtggg                                                               >  1:403069/1‑242 (MQ=255)                                                                                                                                                                                  cacaGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGGG‑ATCATCCCGTGCAAGAAC‑Tc                                                                        >  2:147707/1‑241 (MQ=255)                                                                                                                                                                                    caGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCCC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCCGGAAC‑TCACCggggggatt                                                            >  2:629679/1‑244 (MQ=255)                                                                                                                                                                                            tattGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGACTAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACAACAAGCAGGCGCTGGCGGAG‑AACATCCCGGGCAGGAACCTC‑CCGGGGGAACCGGCATa                                                      >  1:517866/1‑249 (MQ=255)                                                                                                                                                                                            tattGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGGGCTGGCGGGGCATCA‑CCCGGGCAGGAAcacccccggggaaactgcataa                                                      >  2:732270/1‑229 (MQ=255)                                                                                                                                                                                                gttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCCTGCAGGa                                                                             >  1:475332/1‑223 (MQ=255)                                                                                                                                                                                                 ttgCGCCAAACCATGCGCCAAGGGTGTAAAAAGGCAAATTCCGG‑ACCACACTGTTCATCCCCCACCAC‑GGCG‑GGGAAA‑CAAAATAAGCCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGTGGAATCGGCATAAGCGGc                                                <  1:304030/250‑1 (MQ=255)                                                                                                                                                                                                       aaaaCAATGCCCCAAGGCTGAAAAAAGGAAAATTGCGG‑AGCCCTTTGTCCTTCCCCCCCCCC‑GGGG‑GGGAAA‑CAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAATCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGTGGAATCGGCATAAGCGGCATTGGAg                                         <  1:484581/250‑1 (MQ=255)                                                                                                                                                                                                         aacctgacccaaggggtgaaaaaaGGCAAATTGCGG‑ACCCCTCTGTTCTTCCCCCCCCAG‑GGGG‑TGAGAA‑CAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAAGTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑AGCATCCCGTGCAGGAAC‑TCGCCGGTGGAATCGGCATTAGCGGCATTGGAGGc                                       <  2:82291/229‑1 (MQ=255)                                                                                                                                                                                                            cATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCAATCCGGGCAGGAAA‑CCACCGGGGGAATCGGCATAAGCGGCATTTGAGGCAtt                                    >  1:697572/1‑251 (MQ=255)                                                                                                                                                                                                                          gaaaaaaGGAAAATTGCGG‑ATCACCCTTTTCATCCCCCCCCAC‑GGCG‑GGGAAA‑AAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATcgcg                        <  1:629679/244‑1 (MQ=255)                                                                                                                                                                                                                           aaaaaaGGAAAATTGGGG‑ATCCCCCTGTTCTTCCCCCCCCGC‑GGCG‑GGGAAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGTGGAAGCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTc                     <  1:233150/247‑1 (MQ=255)                                                                                                                                                                                                                               aaGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGGGGAATCGGCATAAGCGGCATTGGAGGCCTTGCTTAAATCGCGCTCAggg                 >  1:100121/1‑250 (MQ=255)                                                                                                                                                                                                                                  gaaaaTTGCGG‑ATCCCACTGTTCATCCCCCCCCAC‑GGCG‑GGGAAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgcc              <  1:550735/249‑1 (MQ=255)                                                                                                                                                                                                                                    aaaTTGCGG‑ATCACCCTGTACATCCCCCCCCCC‑GGGG‑TGGAAA‑CAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑AGCATCCCGTGCAGGAAC‑TCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgccag            <  1:112670/251‑1 (MQ=255)                                                                                                                                                                                                                                               tCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGTGGGAACGGCATAAGCGGCATTGGAGGCATTGCTTAAAACGAGCCCAGGCgccaccccccggttt  >  1:55752/1‑240 (MQ=255)                                                                                                                                                                                                                                                 acacTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGGGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTgggtc  >  1:237142/1‑246 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   CAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCC‑ATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGCGG‑ATCACACTGTTCATTCCCCACCAC‑GGCG‑TGGGAA‑CGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTG‑ATCATCCCGTGCAGGAAC‑TCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTC  >  NC_000913/1174356‑1174842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑