Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,457,1050AG28.4% 148.7 / 21.5 81I441V (ATC→GTC) mplUDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
Reads supporting (aligned to +/- strand):  ref base A (4/54);  new base G (22/1);  total (26/55)
Fisher's exact test for biased strand distribution p-value = 8.65e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAA  >  NC_000913/4456858‑4457354
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                               
ggAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGAt                                                                                                                                                                                                                                                                <  2:232005/249‑1 (MQ=255)
ggAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGAt                                                                                                                                                                                                                                                                <  2:567346/249‑1 (MQ=255)
       cgcTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCGGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCGCAGCCGGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGaa                                                                                                                                                                                                                                                       <  2:506485/251‑1 (MQ=255)
               tACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGGTCCATCAGAAA‑ATGCTgg                                                                                                                                                                                                                                              >  1:698592/1‑251 (MQ=255)
                   aTGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATgg                                                                                                                                                                                                                                         <  2:364780/251‑1 (MQ=255)
                         aTGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTTGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGc                                                                                                                                                                                                                                   <  1:342663/251‑1 (MQ=255)
                            gggATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTGGGGGGGTCCATCAGAAA‑ATGGtgg‑gtggggcggggg                                                                                                                                                                                                                                 >  1:518287/1‑243 (MQ=255)
                             ggATCTGCAAAGACGATCTGGCCCCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGTAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTTGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCTTCCATATTCTGGTGATGAGCGGCTGCGG‑TTTGTGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCgaag                                                                                                                                                                                                                               <  1:645292/251‑1 (MQ=255)
                             ggATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCATAAA‑CTGCTGG‑ATGGGCTGGCgaag                                                                                                                                                                                                                               <  2:688440/251‑1 (MQ=255)
                                  tGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGCTGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCCTATTCTGGTGATGAGCAACGGCGG‑GTTTTGTGGGGGCCCTCATAAA‑ATGCTGG‑GTGGGGTGGCGAAGAAggg                                                                                                                                                                                                                          >  1:456317/1‑250 (MQ=255)
                                   gCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAgg                                                                                                                                                                                                                           <  1:720455/249‑1 (MQ=255)
                                     aaaGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGa                                                                                                                                                                                                                       <  1:368859/251‑1 (MQ=255)
                                      aaGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGGTTTTGGGGGGGTC‑ATCAGGAA‑CTGCGGG‑GTGGGGTGGGGGAGAAgggggg                                                                                                                                                                                                                       >  1:107455/1‑246 (MQ=255)
                                       aGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAg                                                                                                                                                                                                                     <  1:206193/251‑1 (MQ=255)
                                                tgCACCTTCATTAGGTCGGGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGT‑ATGGGCTGTCGAAGAAGGCGGAAGCcgcg                                                                                                                                                                                                                <  2:437865/246‑1 (MQ=255)
                                                                          aaGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCTTCAGAAA‑CTGCTGT‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTGAGCCAGATATAGTAt                                                                                                                                                                               <  2:390169/251‑1 (MQ=255)
                                                                            gTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGc                                                                                                                                                                             <  1:151572/251‑1 (MQ=255)
                                                                                 ccTGCTGCAGCCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAgg                                                                                                                                                                        <  2:497626/251‑1 (MQ=255)
                                                                                    gctgcAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGGTACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGGGCCCTCAGAAA‑CCGCTGG‑GAGGGGGGGCGGAGAAGGGGGAAGCCGGGCAG‑G‑AAATC‑GGGCTTTGGCCGATATAGTATGCGGAgggcg                                                                                                                                                                     >  1:644818/1‑248 (MQ=255)
                                                                                    gctgcAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGGGGGGTCCATCAGAAA‑AAACTGG‑GTGGGGTGGGGGAGAAAGGGGAAGGCGCGCCG‑T‑AATTT‑GGGCGTTGCCAGAGATAGTATGCGTAAGCCg                                                                                                                                                                     >  1:500416/1‑251 (MQ=255)
                                                                                       gcAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTAt                                                                                                                                                                               <  1:139030/238‑1 (MQ=255)
                                                                                            cGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGGTCCATCCGAAA‑ACGCTTG‑ATGGGCTGGCGAAGAAGGGGGGAGCCGCGCCG‑G‑AAATC‑GGCCTTAGCCCGATATAGTATGCGTAGGGCGGATaaagg                                                                                                                                                             >  1:523977/1‑248 (MQ=255)
                                                                                               cgcATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCtt                                                                                                                                                                                              <  2:51448/215‑1 (MQ=255)
                                                                                                  atatTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCTTAGGCCGGATAAGTCGTTCAc                                                                                                                                                       <  1:573811/251‑1 (MQ=255)
                                                                                                  atatTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCAc                                                                                                                                                       <  1:668884/251‑1 (MQ=255)
                                                                                                             caggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGGGGGGGCCATCAGAAAACT‑CTGG‑GTGGGCTGGGGGAGAAAGCGGGAGCCGCGCCG‑T‑AATTCCGGCCT‑AACCCGATATAGTATGCGGAGGGCCGATAAGGGGTTCACGCCGCaatcgg                                                                                                                                            >  1:17042/1‑246 (MQ=255)
                                                                                                                gtggcagaagtggcagaaGCCTGCGTTCAGCCGGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATGCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAGGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATATGATGCGTGGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                                                                                                                         <  2:701061/251‑1 (MQ=255)
                                                                                                                            gcagaagCCTGCGGTCAGCCTGCACACTGGAGTGGCGATGGGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTg                                                                                                                            <  2:583626/251‑1 (MQ=255)
                                                                                                                             cagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGTGGGGGCCATCAGAAA‑ATTCTGG‑GTGGGGTGGCGAAGAAAGCGGAAGCCGCGCAG‑G‑AATTT‑GGCCTTAGCCAGATATAGTATGCGGAGGCCGGAGAAGGGGGTCACGCCGCAACCCGCATTTGAGGATA‑GTGc                                                                                                                           >  1:626309/1‑251 (MQ=255)
                                                                                                                              agaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGcc                                                                                                                                                                      <  1:140991/208‑1 (MQ=255)
                                                                                                                              agaagCCTGCGTTCAGCCTGCACAATGGCGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGGACCCTCAGCAA‑ATGCTGGGATGG‑CTGGGGGCGAAAGGGGGAGCCGCGCAG‑T‑AAATT‑TGCCTTTGCCCGAGATAGTATTCGTAGGCCGGGTAAAGCGGTCACGCCGCATCCGGCCTTTTAGCATA‑gagac                                                                                                                          >  1:639312/1‑247 (MQ=255)
                                                                                                                               gaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑ATTTGGGGGGGGCCCTTCGAAA‑CTGTCGG‑GTGGGGTGGTGGAGAAGGCGGGAACCGCGCGGGA‑AATTG‑GGCCT‑AGCCAGAAATAGTATGCGGAGGGGGGGTAAGGGGCTCACGCCGCACCCGGCGCTTGATCATA‑GTggcc                                                                                                                         >  2:548398/1‑248 (MQ=255)
                                                                                                                                           cAGCCTGCACACTGGAGTGGCGATGTGGATCCGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCtt                                                                                                             <  1:198311/251‑1 (MQ=255)
                                                                                                                                           cAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATAGGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGT‑TTTTGGTGGGATCCATCAGAAT‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCATTCTCGTGGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCt                                                                                                              <  1:682019/250‑1 (MQ=255)
                                                                                                                                           cAGCCGGCACACGGGGGGGGCGATGTGGATACGCGGGACGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCAGATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGATCCATCAGAAA‑CGGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCt                                                                                                              <  2:321923/250‑1 (MQ=255)
                                                                                                                                            aGCCTGCACACTGGAGGGGCGATGGGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTg                                                                                                            <  2:121108/251‑1 (MQ=255)
                                                                                                                                                     acTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGTCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCtt                                                                                                   <  1:373448/251‑1 (MQ=255)
                                                                                                                                                       gggAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTAt                                                                                                 <  2:518287/250‑1 (MQ=255)
                                                                                                                                                        ggAGTGGCGATCTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGGTCCATCAGAAA‑ATGCTGG‑ATGGGCTGGGGCAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCCGATATAGTATGCGTAGGGCGGGTAAGGCGTTCACGCCGCCTCCGGCATTTTAGCATA‑GTGCCCGATGCGACCCTTGGTGCGTCTTATc                                                                                                >  1:168788/1‑251 (MQ=255)
                                                                                                                                                          aGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑ATTTGTGGGGAACCATAAGAAA‑CTGCTGG‑GTGGGGTGGAGCGGACAGCGGGAGCCGCGCAG‑G‑AAATT‑GGCCTTAGCCCGAAATAGGCTGCGTATGGCGGATAAGGGGTTCACGCCGCATCCGGCTATTGTGCATA‑GTGCCTGAGGCGACGCTTGATGAGTCTTATc                                                                                                >  1:474112/1‑249 (MQ=255)
                                                                                                                                                            tGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGTGGGGGCCCTCAGAAA‑CTGCTGG‑GTGGGGTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTTGCCCGATATAGGATGGGGAGGCCGGATAAAGGGGTCACGCCGCATCCGGCAGTTTAGCATA‑ATGCCTGATGCGGCGCTTGATGCGTCTTATCAggg                                                                                            >  1:590103/1‑250 (MQ=255)
                                                                                                                                                            tGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGAGATAGTATGCGTAGGcc                                                                                                                                                                      <  1:228939/178‑1 (MQ=255)
                                                                                                                                                             ggCGATTTGGATACGCTGGCAGATATGGTGGTGAAAACCTCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGTGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAGGAAGGCGGAAGCCGCGCGG‑T‑AATTC‑GGCTTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGTCATTTGATCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGcc                                                                                           <  2:733117/251‑1 (MQ=255)
                                                                                                                                                                gaggtgGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTAc                                                                                        <  1:390336/248‑1 (MQ=255)
                                                                                                                                                                   gtgGATACGCTGGCAGTTATTGTTGTGAAAACCGCTCAGCCGGGCGACCATATTCTGGTGAGGAGCAACGGCGG‑TTTTGGTGGGATCCTTCAGAAA‑CTGCTGG‑ATGGGCTGGCTAAGAAGGCGGAAGCCGCGCAG‑T‑AATGC‑GGCCTTATCCAGATATAGTAGGCGTAGGCCGGATAAGGCTTTTACGCCGCATCCTGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAgg                                                                                     <  2:644818/251‑1 (MQ=255)
                                                                                                                                                                      cgcgtccgatctgATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAC‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCTCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGc                                                                                  <  2:97557/239‑1 (MQ=255)
                                                                                                                                                                        tACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGGTCCCTCAGGAA‑CTGCTGG‑GTGGGCTGGCGGAGAAGGCGGAAGCCGCGCAG‑G‑AATTC‑GGCCCTAGCCAGATATAGTATGCGGAAGCCGGGTAAGGCGGTCACCCCGCCTCCCGCCTTTTAGCATA‑GGGCCTGATGCGGCGCTTTATGCGTCTTATCCAGCCTACAgggggcc                                                                                >  1:161910/1‑246 (MQ=255)
                                                                                                                                                                         aCGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCAcc                                                                               <  1:550825/251‑1 (MQ=255)
                                                                                                                                                                         aCGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGATGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCAcc                                                                               <  1:209408/251‑1 (MQ=255)
                                                                                                                                                                          cGCTTGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGTTGAGGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAACCCGCGCAG‑T‑AATGC‑GGCCTTAGCCAGATATAGTAGGCTTAGGCCTGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCAcc                                                                               <  1:306156/250‑1 (MQ=255)
                                                                                                                                                                            cTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGa                                                                                                                                                                   <  1:163484/165‑1 (MQ=255)
                                                                                                                                                                            cTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGGTCCCTCACAAA‑ATGGCGG‑AGGGGGTGGCGAAGAAAGCGGGAGGCGCGCAG‑T‑AATTT‑GGGCTTAGCCAGATATAGTATTAGGAGGCCGGGTAAAGCGGTCCCGCCGCATCCGGCATTTGAGCATA‑GTGCCCGGTGGGGCGCTTGTTGCGTGTTATCAGGGCTAccagggcaccggt                                                                            >  1:313331/1‑239 (MQ=255)
                                                                                                                                                                             tGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGT‑TTTTGGTGGGGTCCATCAGAAA‑CTGGTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGTATAAGGCGTTc                                                                                                                                                         <  1:454911/174‑1 (MQ=255)
                                                                                                                                                                              ggctgatttggtggTGAAACCCGCTCAGCCTGGCGCCCATATTCTGGTGATAAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑ATGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCCGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATc                                                                          <  2:180123/243‑1 (MQ=39)
                                                                                                                                                                               gCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATcc                                                                         <  1:151401/251‑1 (MQ=255)
                                                                                                                                                                                  gATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGATCCATCCGAAA‑ATGGTGG‑GTGGGCTGGGGGAGGAGGGGGGAGGCGGGGAG‑G‑AATTC‑GGCCTTAGCCAGATATAGTATTCGGAGGCCGGAGAAGGGGGTTACGCCGCCTACGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATgtggtttatctgggccacaggggc                                                                                  >  1:97557/1‑216 (MQ=255)
                                                                                                                                                                                     atggtgtggAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑AGGGGCTGGCGAAGAAGGCGGAGGCCGCGCAG‑G‑AATTC‑GGCCTTAGCCGGATATAGGTTGCGTAGGCCGGATAAGGCGTTCACGCCGCTTGCGGGATTGGAGCAGA‑GTGCCTTATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCaaa                                                                   <  1:57774/243‑1 (MQ=255)
                                                                                                                                                                                       ggtggtGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAAcc                                                                 <  2:472240/251‑1 (MQ=255)
                                                                                                                                                                                        gtggtgAAAACCGCGCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCa                                                                <  2:265077/251‑1 (MQ=255)
                                                                                                                                                                                           gtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGACTACAGGTGCACCGTATCCGGCAAACCATAc                                                             <  2:626309/251‑1 (MQ=255)
                                                                                                                                                                                           gtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCGGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTCCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATAc                                                             <  1:695237/251‑1 (MQ=255)
                                                                                                                                                                                              aaaaCCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGGGCCATCAGAAA‑CTGCTGG‑GTGGGCTGGCGGAGAAAGCGGGAGCCGCGCCG‑TTAATT‑‑GGCCTTAGCCCGGTATTGTTTGGGTAGGCCGGGTAAGGCGTTCCCGCCCCATCCCGCCTTTTAGCAAA‑GTGCCTGATGCGACGCTTTGTGCGTCTTATCAGGCCTCCAGGGGCACCCTATCCCGCaaaacctcctcc                                                          >  2:104643/1‑242 (MQ=255)
                                                                                                                                                                                                 aCCGCTCAGCCGGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACAt                                                       <  2:456317/251‑1 (MQ=255)
                                                                                                                                                                                                       cAGCCTGGCGACCAAATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCAGCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCTGCAAACCATACTCACATcaaca                                                  <  1:590896/250‑1 (MQ=255)
                                                                                                                                                                                                        aGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TCTTGGTGGGGTCCATCAGAAA‑CCGCTGG‑GTGGGAATGCGAAGAAGGGGGAAGCCGCGCAG‑A‑AATTC‑GGCCTTAGCCCGCTATAGTATTCGGAGGCCGGATAAGGGGTTCACGCCACATCCGGCAATTGAGCATA‑GGGCCTGAAGCGACGCCTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATTCGGCAAACCATACTCACATcaaacac                                                >  1:640163/1‑247 (MQ=255)
                                                                                                                                                                                                            tGGCGACCATATTCTGGTGATGAGCAACGGCGG‑GTTTGGTGGGAACCATACGGAA‑CTGCTGG‑GCGGGCTGGCGGAGAAAGGGGAAGCCGCGCAG‑G‑AATTC‑GGCCTTAGCCCGAAATAGTATGCGTAGGCCGGATAAGGCGTTCAAGCCCCATCCGGCATTTGAGACTA‑GTGCCCGATGCGACGCTTGATGCGTCTTATTAGGCCCACCGGTGCCCCCTATCCGGCAAACCAAACTCACAACCACAACGac                                             >  1:730264/1‑249 (MQ=255)
                                                                                                                                                                                                             ggCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGaaaa                                           <  1:9179/251‑1 (MQ=255)
                                                                                                                                                                                                                gACCATATTCTGGTGATGAGCAACGGCGG‑TTTTTGGGGGGTTCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGGAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCCGATATAGTATGCGTAAGCCGGATAAGGGGTTCACGCCGCATCCGGCATTTGTGCCTA‑GTGCCTGGTGGGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCCTACTCACATCAACAACCaaaaatt                                        >  1:113603/1‑248 (MQ=255)
                                                                                                                                                                                                                 aCCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGGGGGGTCCCTAAGACA‑CTCCTGG‑GTGGGCCGGCGGAGAAGGCGGGAGCCGCCCAG‑G‑AATTC‑GGCCTTTGCCCGCTATTATATGCCTAGGCCGGAGAAGGCGTTACCGCCCCCTCCCGCATTTTAGCATAAGT‑CCTGATGGGACGCTTTGTTCCTCTTATCTGGCCCACAGGTGCACCGTATCCGGCCAACCATAcccccaccaccaaaaaaaactt                                        >  2:704255/1‑229 (MQ=255)
                                                                                                                                                                                                                 aCCATATTCTGGTGATGAGCAACGGCGG‑TTTGGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCGGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTAc                                       <  2:577328/251‑1 (MQ=255)
                                                                                                                                                                                                                    atatTCTGGTGCTGAGCAACGGCGG‑GTTTGGTGGGGTCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGCCGAAGGGGGAAGCCGCGCAG‑T‑AATTC‑GGGCCTAGCCAAACATAATATGGGTTGGCCCGGTAAGGGGGTCACGCCCCATCCGGCATTTTAGCCTA‑GTGCCTGACGCACCGCTTGATGCGGCTTTTCCGGCCCACACGGGCACCCGATCCGGCACACCCTACTCCCCTCAACAACGAAAATTAcccc                                    >  2:102432/1‑250 (MQ=255)
                                                                                                                                                                                                                           ggTGATGAGCAACGGCGG‑TTTTTGGGGGGTCCATCAGAAA‑ATGCTGG‑ATGGGGTGGCGAAGAAAGCGGAAGCCGCGCAG‑T‑AATTT‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGGTAAGGCGTTCAAGCCGCATCCGGCATTTGAGCATA‑GTGCCTGGTGCGACGCTTGATGCGTCTTATCAGGCCTACAGGGGCACCGTATCCGGCAAACCATAATCACATCAACAACGAAAATTACCCCTCGTtct                             >  1:245248/1‑251 (MQ=255)
                                                                                                                                                                                                                           ggTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTtct                             <  1:311691/251‑1 (MQ=255)
                                                                                                                                                                                                                            gTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTtctc                            <  1:79833/251‑1 (MQ=255)
                                                                                                                                                                                                                               atgaGCAACGGCGG‑TTTTTGTGGGGTCCCTCAGAAA‑CTGCTGG‑GTGGGCTGGCGAAGAAAGCGGGAGGCGCGCCG‑T‑AATTT‑GGCCTTAGCCAGGTATAGTATGCGTAGGGCGGATAAGGCGGTCACGCCCCATCCGGCATTTTAGCATA‑ATGCCTGATGCGACGCCTGGTGCGTCTTATCAGGCCTACCGGGGCACCGTATCCGGCAAAACATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGc                         >  1:423264/1‑251 (MQ=255)
                                                                                                                                                                                                                                 gagtaaCGGCGG‑TTTTGGGGGGATCCATCGAAAA‑CTGCTGG‑ATGGGCTGGGGAAGAAGGCGGAAGGCGCGCGG‑T‑AATGC‑GGCCTAAGCCAGATATAGTATGCGTATGCCGGATAAGGCTTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGGCGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTCCCCTTCGTTCTCCGCt                        <  2:556564/246‑1 (MQ=255)
                                                                                                                                                                                                                                  aGCAACGGCGG‑TTTTGGTGGGAGCCACCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTaa                      <  2:698592/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAAc                     <  1:162976/251‑1 (MQ=255)
                                                                                                                                                                                                                                         gcgg‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCgcgc               >  1:703087/1‑251 (MQ=255)
                                                                                                                                                                                                                                            g‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGa            <  2:168788/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       gATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGaaaa  <  2:544337/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                               
GGAGCCGCGCTCGAATACCATGAAAATGGGGATCTGCAAAGACGATCTGGCACCTTCATTAGGTCGTGCCGATGAAGTCTTCCTGCTGCAACCGGCGCATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGG‑TTTTGGTGGGATCCATCAGAAA‑CTGCTGG‑ATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAG‑T‑AATTC‑GGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATA‑GTGCCTGATGCGACGCTTGATGCGTCTTATCAGGCCTACAGGTGCACCGTATCCGGCAAACCATACTCACATCAACAACGAAAATTACCCTTCGTTCTCCGCTAACTCGCGCAGATACTGGAAAA  >  NC_000913/4456858‑4457354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑