Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	97.8%	150.3 / ‑3.7	46	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (23/21);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

CGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑GTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGA‑TGAAGAGCCGCGCCGTCGACACGGTTTCTGGC  >  NC_000913/1905517‑1906006                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       cGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGcc                                                                                                                                                                                                                                                    <  2:14293/249‑1 (MQ=255)         aaCGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCATCCTCCATa                                                                                                                                                                                                                                          <  1:160514/251‑1 (MQ=255)            ggggCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCAATGCCTGCCTGCACGCGAGAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGTTGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGCTGGATGCATTTTCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAAc                                                                                                                                                                                                                                       >  2:273615/3‑251 (MQ=255)                   gTACGGACTGAATAAATTCAGTCCGGCGAGACCATCTGCACATCACTCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCCTGTATGCATGTTTGCTGGGGGCGCTGATGTGTTTTATGGATAACCCGGTCAGGAAATTGTCATGAATCTCACTGCTACTGTTCTTCTTGTGTTTGGTATGTCGATGGATGCATTTTCTGCATCAATCGGTCACG‑ATGCCCCCCTCCATAAACCGCAAtt                                                                                                                                                                                                                                >  2:286414/1‑251 (MQ=255)                        gACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCACTCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGGTCTTCTTGCGTTTGGTATGTCGAGGGAGGCATTTGCTGCATCAATCGGTAAAGAGT‑CCACCCGCCATAAGCCGAAATTTTCTg                                                                                                                                                                                                                           <  2:40906/251‑1 (MQ=255)                                  tttCAGTCAGGCGAGAACATATGCACATCAATCGCTATGCCTGCATGAACGCGCAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGTTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCg                                                                                                                                                                                                                 >  2:562815/1‑251 (MQ=255)                                                gaCCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAttt                                                                                                                                                                                                   >  1:142539/1‑251 (MQ=255)                                                          acaTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAATCCGAAATTTTCTGAAGCAtt                                                                                                                                                                                                                    <  1:204443/224‑1 (MQ=255)                                                          acaTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                                                                                                                                                                                                                    >  2:204443/1‑224 (MQ=255)                                                           caTCAAGCGCTAGGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCGTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATTCCACCCTCCATAAACCTAAATTTTCTGAAGCATTGCGAACCTGCCTTATTTTTGGTGCCGTc                                                                                                                                                                                        <  2:555516/251‑1 (MQ=255)                                                             tCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATCCCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGACGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCg                                                                                                                                                                                       >  2:361018/1‑250 (MQ=255)                                                               aaTCGCTATGCCTGCATGAACGCGAAAACCATCACTGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTACTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGaaa                                                                                                                                                                                    <  2:429749/251‑1 (MQ=255)                                                                   gCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCt                                                                                                                                                                                >  1:7206/1‑251 (MQ=255)                                                                         ccTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGCCATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgcc                                                                                                                                                                          >  1:48803/1‑251 (MQ=255)                                                                         ccTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgcc                                                                                                                                                                          >  1:179113/1‑251 (MQ=255)                                                                         ccTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTAGGCATGTTTGCTGGGGGCGAGGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGAcgcc                                                                                                                                                                          <  1:562815/251‑1 (MQ=255)                                                                                gAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCTCCCTCCAGAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGAt                                                                                                                                                                    <  1:100200/250‑1 (MQ=255)                                                                                   cgcgAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGCAACCCTGACGCCGCTGATCGGc                                                                                                                                                                >  2:448658/1‑251 (MQ=255)                                                                                    gcgAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGACCGGCt                                                                                                                                                               >  2:226936/1‑251 (MQ=255)                                                                                      gAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTgg                                                                                                                                                             >  1:341690/1‑251 (MQ=255)                                                                                                         tgttCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGAGCATGTTagcc                                                                                                                                           <  2:123287/250‑1 (MQ=255)                                                                                                          gttCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCAACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTagcc                                                                                                                                           >  1:97953/1‑249 (MQ=255)                                                                                                                  aTGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGT‑AGCCAGCCGGTTTGt                                                                                                                                >  1:90047/1‑251 (MQ=255)                                                                                                                        gTTTGCTGGGGGCGATGAGGTGGTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTg                                                                                                                           <  2:29344/251‑1 (MQ=255)                                                                                                                             gtgggggggaggattggtTTGATGGATACCCCGTTCAGGACATTGTCAGGAATATCACTTCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTGATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCTGTTTGTCCTTGAATgg                                                                                                                      <  2:314712/235‑1 (MQ=255)                                                                                                                                 gggCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAAcc                                                                                                                  >  2:104015/1‑251 (MQ=255)                                                                                                                                  ggCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGCATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAAcc                                                                                                                  >  2:418773/1‑250 (MQ=255)                                                                                                                                     gatgatGTGTTTTAGGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTg                                                                                                              <  1:493221/251‑1 (MQ=255)                                                                                                                                           gtgtTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTg                                                                                                         <  1:226936/250‑1 (MQ=255)                                                                                                                                               tttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGttt                                                                                                    >  2:105886/1‑251 (MQ=255)                                                                                                                                                ttATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTtg                                                                                                   >  1:205766/1‑251 (MQ=255)                                                                                                                                                    ggATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTCTGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGACATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgct                                                                                               >  2:559754/1‑251 (MQ=255)                                                                                                                                                    ggATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCAGCAAACGGTAAAT‑ATGCCACCCTCCATAAACCGAAAGTTTCGGAAGCATTGCGAACCGGCCGTATTGTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCTGTTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGtgc                                                                                                <  1:306559/250‑1 (MQ=255)                                                                                                                                                                      aTTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTcggcgg                                                                             <  1:510930/251‑1 (MQ=255)                                                                                                                                                                           cATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGGACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCggcggggggg                                                                        >  2:133497/1‑247 (MQ=255)                                                                                                                                                                              gAATATCACTGCTACTGTTCTGCTTTCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGGAAAG‑AGGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGGGTGTGCTGCTGATATTCCGCGGTGGGCGAATg                                                                      <  2:44286/250‑1 (MQ=255)                                                                                                                                                                                    cACTGCTACTGTTCTTCTTGAGTTTGGTCTGTCGATGGATGCATTTGATGCCTCAATCGGTAACG‑ATGCCACCCTCCATAAACCGCAATTTTCTGAAGCATTGCGCACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAACGGGAATGTTAGCCAGCCGGTTTGTCCTTGACTGGAACCACTGGCTTGCGTTTGTGCTGCTGATTTTCGTCGGCCGGGGACTGattatgt                                                               >  2:575166/1‑249 (MQ=255)                                                                                                                                                                                           aCTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGTAACCACTGGATTGCGGTTGTGCGGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGttt                                                        <  1:76389/251‑1 (MQ=255)                                                                                                                                                                                                  ttcttGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGGATGATTATTGAGGGGTTTCGTggg                                                 >  1:356336/1‑250 (MQ=255)                                                                                                                                                                                                         gTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAgatg                                          <  1:105886/251‑1 (MQ=255)                                                                                                                                                                                                            tGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGT‑AGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGatgatg                                       <  2:90047/250‑1 (MQ=255)                                                                                                                                                                                                                  gTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTTTCTGACGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCg                                                                                                                                                                  >  1:275997/1‑122 (MQ=255)                                                                                                                                                                                                                  gTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑ATGCCACCCTCCATAAACCGAAATTGTCTGAAGCATTGCGAACCGGCCGTATTTTTGGTGCCGTCGAAACCCTGACGCCGCGGATCg                                                                                                                                                                  <  2:275997/122‑1 (MQ=255)                                                                                                                                                                                                                              aTTTGCTGCATCAATCGGGAAAG‑ATGCCCCCCTCCATAAACCGAAATTTTCCGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGGCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGA‑TGAAGAGCCGCGcc                    <  2:429048/251‑1 (MQ=255)                                                                                                                                                                                                                                          aaTCGGTAGAG‑CTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGGATGGTTATTTAGGGGTTTTGTGGGGGAGTTGATGA‑GAGTTAAGAGGCGCGCCGTCggcgcgggt        >  2:212579/1‑243 (MQ=255)                                                                                                                                                                                                                                                 aaaG‑ATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGA‑TGAAGAGCCGCGCCGTCGACACGGTTTCTGGc  <  1:380687/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CGTTGCAAAACGTGGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAG‑GTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGA‑TGAAGAGCCGCGCCGTCGACACGGTTTCTGGC  >  NC_000913/1905517‑1906006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑