Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,279,023	T→C	F182S (TTC→TCC)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,023	0	T	C	98.0%	165.5 / NA	49	F182S (TTC→TCC)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (26/22);  minor base A (1/0);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCT  >  NC_000913/4278778‑4279269                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gCTGGGGCAACATATTAGCGTACCGGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGAGGGTCTGCCCGTTGCGCTGTGTGATTTCGCGACCTCCCCg                                                                                                                                                                                                                                                    <  2:384960/250‑1 (MQ=255)    ggggCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGAGGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGt                                                                                                                                                                                                                                                  <  2:595634/249‑1 (MQ=255)     gggCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAt                                                                                                                                                                                                                                               <  1:504705/251‑1 (MQ=255)              atTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGGGATTTCGCGTCCTCCCCGGTGAT‑‑TGTCACTgg                                                                                                                                                                                                                                    >  2:566690/1‑251 (MQ=255)              agtAGCGTACCTGTCGGGCTGGGTGCCGTTTTCCTGAAGGGTGTGCTTTTTACGGTATTTTCTGCCACGGGTATCCGTAGCTGGAGTTTGCGCACCTTGCCTCACGGTGTGGCTCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCTCTAACGGTTTCGGTCTTTTGATTAAAGTCCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAttgtcactt                                                                                                                                                                                                                                       <  2:7659/248‑8 (MQ=255)                 aGCGTACCTGTCGCGCTGGGTGCCGAGGTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGATGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGCTTTCGCGACCTCCCCGGTGAttgtaactggt                                                                                                                                                                                                                                     >  2:53828/1‑240 (MQ=255)                      aCCTGTCGCGCTGTGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGAAATTTCTGCCACGGGTGTCCGTAGCTGGATTTTGCGCAAATTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGAATCGTTCTGTTCCTGCTGCTCATTGCCGTTAACGGTGGCGGTCTGGTGCTTAAAAACGCGCTTGATGGTCTACACGTTGCGGTGGGGGATTTCGCGACCTACCCGGTGAT‑‑TGTCACTGGTAggatat                                                                                                                                                                                                                            >  1:312763/1‑247 (MQ=255)                            cgcgCTGGGTGCCGTGTTCCTTATGGGTGGGCTGTTTACGGTAATTTCGGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGTTGTGGCGCACGGCACGGGGATTGGTATCTGTCTGTTCCTGCTGCTCATTGCCGCTAACGGGGTCGGTCTGGTGATTAAAGACCCGCTGGATGGTCTGCCCGTTGCGCGGGGTGATAGCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCataccta                                                                                                                                                                                                                       <  1:578340‑M1/250‑8 (MQ=255)                                  gggTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgac                                                                                                                                                                                                                  <  1:516271‑M1/249‑13 (MQ=255)                                      gCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGGCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGATCTCCCCGGTGAT‑‑TGTCACTGGTAGGGCataccgaacgacaacagg                                                                                                                                                                                                            <  2:350809‑M1/251‑19 (MQ=255)                                        cGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCTGTAGCTGGGTTTTGCGCAACTTGCCTCACGGGGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGCCCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgccaccaggt                                                                                                                                                                                                           >  2:519991‑M1/1‑231 (MQ=255)                                         gtgtTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaa                                                                                                                                                                                                         <  1:227978‑M1/251‑22 (MQ=255)                                                    gggTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatac                                                                                                                                                                                              <  2:14146‑M1/251‑33 (MQ=255)                                                     ggTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGATT‑T‑TCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacc                                                                                                                                                                                             <  1:520839‑M1/251‑34 (MQ=255)                                                      gtgtGCTGTTTCCGGTACTTTATGCCACGGGTATCCGCAGCAGGATTTTGCGCAACTTGACTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCATGCTGCTCATTGCCGATAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgcccacaggaaaaaaaaccataccc                                                                                                                                                                                            >  2:355974‑M1/1‑217 (MQ=255)                                                        gtgCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccataccca                                                                                                                                                                                           >  1:343786‑M1/1‑215 (MQ=255)                                                          gCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaa                                                                                                                                                                                                        >  2:149462‑M1/1‑213 (MQ=255)                                                          gCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaa                                                                                                                                                                                                        <  1:149462‑M1/235‑23 (MQ=255)                                                             gTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTTATTAAAAACCCGCTTGATGGGCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACGGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaag                                                                                                                                                                                       <  1:383041‑M1/249‑40 (MQ=255)                                                                   ggTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtga                                                                                                                                                                                >  1:345815‑M1/1‑204 (MQ=255)                                                                             gCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGTCCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgc                                                                                                                                                                       <  1:566690‑M1/249‑56 (MQ=255)                                                                             gCCACGGGTATCCGTAGCTGGATGTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaactacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttg                                                                                                                                                                        <  1:352064‑M1/248‑55 (MQ=255)                                                                                    ggatcCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGGCGGTCTGGTGAGTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTGCGCGACCTCCCCGGTGAT‑‑TGTCACTTGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactg                                                                                                                                                              <  2:27557‑M1/249‑65 (MQ=255)                                                                                               tGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaacaaaccatacccaaaaggtggggaatcgtttgcttttaactggggattgttc                                                                                                                                                    >  2:177060‑M1/1‑176 (MQ=255)                                                                                                ggATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttc                                                                                                                                                    <  1:173907‑M1/249‑75 (MQ=255)                                                                                                       gcgcAACTTGCCTCACGGTGGGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGTTGATTAAACACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGAGTTCGCGACCGCCCCGGTGAT‑‑TGTCACTGGTAGGTCatgcctaactacaacaggaaaaaaaaccattcccaaaatgttgttaatcgtttgcttttaactggggattgttcaggtggcg                                                                                                                                            <  1:341298‑M1/250‑83 (MQ=255)                                                                                                              ttGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtg                                                                                                                                    >  1:286570‑M1/1‑161 (MQ=255)                                                                                                                    cACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcagctggcgttgcggtgtttt                                                                                                                                <  1:519991‑M1/249‑95 (MQ=255)                                                                                                                     aCGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatccctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttga                                                                                                                              >  1:261566‑M1/1‑154 (MQ=255)                                                                                                                       ggTGTGGCGCACGGCCCGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggaatgttcaggtggcgttgcggtgttttgaatg                                                                                                                           >  2:149434‑M1/1‑152 (MQ=255)                                                                                                                       ggTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatg                                                                                                                           >  1:136175‑M1/1‑152 (MQ=255)                                                                                                                        gtgtGGCGCACGGCACGGGGAATGGTATCGGTCTGTTCATGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgt                                                                                                                          >  2:399641‑M1/1‑151 (MQ=255)                                                                                                                              ggcacggcacggGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagac                                                                                                                    <  2:97804‑M1/250‑107 (MQ=255)                                                                                                                                   ggcacggGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGATCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggcagtgaatcgtttgtttttaactggggattgttcaggtggcgttgcggtgttttgtatgttaagactgtgg                                                                                                               >  2:15968‑M1/1‑140 (MQ=255)                                                                                                                                              tGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaat                                                                                                     <  2:269716‑M1/250‑122 (MQ=255)                                                                                                                                                              ctgctgCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggcaccgcc                                                                                    >  2:556055‑M1/1‑113 (MQ=255)                                                                                                                                                               tgctgcTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggatccgcct                                                                                   >  2:55583‑M1/1‑112 (MQ=255)                                                                                                                                                                gctgctCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctc                                                                                  >  1:237412‑M1/1‑111 (MQ=255)                                                                                                                                                                       aTTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTATGCCCGTTGCGCTGGCTGATTTCGCGACCTCCCCGGTGAT‑‑TGACACTGGTAGGTCatacctaacgacaacaggaaaaacaaccatacccaaaaggtagtgcatagtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggttt                                                                            >  1:577685‑M1/1‑104 (MQ=255)                                                                                                                                                                        ttGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtat                                                                            >  1:379528‑M1/1‑103 (MQ=255)                                                                                                                                                                             gCTAACGGTGTCGGTCTGGTGATTAAAATCCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccat                                                                                                                                                                                                <  2:31258‑M1/128‑31 (MQ=255)                                                                                                                                                                             gCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggacaaaaaaccat                                                                                                                                                                                                >  1:31258‑M1/1‑98 (MQ=255)                                                                                                                                                                                   ggTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtg                                                                                                >  1:476820‑M1/1‑92 (MQ=255)                                                                                                                                                                                   ggTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCGGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtg                                                                                                <  2:476820‑M1/218‑127 (MQ=255)                                                                                                                                                                                          gTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctgggataggggggggcggagatagc                                                         >  2:337649‑M1/1‑85 (MQ=255)                                                                                                                                                                                            cTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTCGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcctt                                                      >  2:244105‑M1/1‑83 (MQ=255)                                                                                                                                                                                                    tAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccggcactggtatcgggggaggcgaagatagcattatttcctc                                              >  2:357573‑M1/1‑75 (MQ=255)                                                                                                                                                                                                                               tGCGCTGGGTGATTTCGCGACCTCCCCGGTGAT‑‑TGTCACTAGTAGGTCctacctaacgacaacaggaaaaaaacccatacccacaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctcttcttcgctgtaccagataac                   >  1:38759‑M1/1‑48 (MQ=255)                                                                                                                                                                                                                                                 gACCTCCCCGGTGAT‑‑TGTCACTGGCAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggcagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctcttcttcgctgtaccagataaccggcaccattaatttat  >  1:221205‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGCCAAGCGTTCTGGCGCT  >  NC_000913/4278778‑4279269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑