BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,523,800	C→G	Y310* (TAC→TAG)	mrcA →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,523,800	0	C	G	100.0%	590.2 / NA	157	Y310* (TAC→TAG)	mrcA	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (87/70); total (87/70)

Only 100 of 157 total aligned reads displayed.

GCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGG  >  NC_000913/3523557‑3524045

gCTGGATGAAGGGTATATCACACAACAACAGTTCGATCAGACTCGCTCTGAGGCGATTCCCGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATCGCGAAAGTGCATATGAAGACGGTTATCGCATTTAACCCA‑CCATCACCTGCAAAGTGCAGAAGGACGAGCAGCAGGCTGTGCGTTATAACGTGCTGGACTAGGCCATgc                                                                                                                                                                                                                                                >  2:542920/1‑251 (MQ=255)

   ggATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAGCTATCACGCGCCGGAGATTGCTGTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGcc                                                                                                                                                                                                                                             <  2:243104/251‑1 (MQ=255)

   ggATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATgcgc                                                                                                                                                                                                                                              <  1:458705/250‑1 (MQ=255)

   ggATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATgcgc                                                                                                                                                                                                                                              <  1:288994/250‑1 (MQ=255)

   ggATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATgcg                                                                                                                                                                                                                                               <  1:199293/249‑1 (MQ=255)

      tGAAGGGTATATCACCAACCACCAGTTCGATCAGCCACGCACTGAGGCGATTAACGCTAACTCTCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGCGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGACAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACg                                                                                                                                                                                                                                          >  2:566102/1‑251 (MQ=255)

         aGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTGGGACATGCGCCACGGCt                                                                                                                                                                                                                                       >  1:323424/1‑251 (MQ=255)

         aGGGTATAGCACCCAACAACAGTTCGATCAGACGCGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGTCTAGGACATGCGCCACGGCt                                                                                                                                                                                                                                       <  2:310987/251‑1 (MQ=255)

            gTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCGGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATc                                                                                                                                                                                                                                    <  1:317949/251‑1 (MQ=255)

              atatCACCCACCAACAGTGCGGTCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGGCGGAGATTGCTGTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATGACGTGCTGGACTAGGACATGCGCCACGGCTATcgc                                                                                                                                                                                                                                  <  2:301051/251‑1 (MQ=255)

               tatCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATcgcg                                                                                                                                                                                                                                 >  2:15179/1‑251 (MQ=255)

                  cACCCAACAACAGTTCGATCAGACACGCACTGAGGCGCTTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGcc                                                                                                                                                                                                                              >  1:240204/1‑251 (MQ=255)

                   aCCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGccc                                                                                                                                                                                                                             <  1:452558/251‑1 (MQ=255)

                      caacaaCAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCCGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTCGGACATGCGCCACGGCTATCGCGGCCCGGc                                                                                                                                                                                                                          >  2:555500/1‑251 (MQ=255)

                      caacaaCAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGc                                                                                                                                                                                                                          >  2:303561/1‑251 (MQ=255)

                          aacaGATCGATCAGACACGCACTGAGGCGATTACCGCTCACTATCACGCGCCGGCGATTGCTTTCTCTGCGCCGTACCTGAGCGACCTGGTGCGCCAGGAGATGTATAACCGTTATGGCGACAGTGCCTATGATGACGGTTAACGCATTTACACCA‑CCATCACCCACAAAGTGCCGCAGGCCGAGCCGCAGGCGGTACGTTATTACGTGCTGGCCTAGGAAATGCGCAACGGCCAGCGCGGCCCGGCCAAt                                                                                                                                                                                                                      >  2:537725/1‑251 (MQ=255)

                             aGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAAtgtg                                                                                                                                                                                                                   >  1:351777/1‑251 (MQ=255)

                                tCGATCAGACACGCACTGAGGCGATTACCGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGcgg                                                                                                                                                                                                                >  2:398597/1‑249 (MQ=255)

                                     cAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGa                                                                                                                                                                                                            >  2:496368/1‑250 (MQ=255)

                                     cAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGa                                                                                                                                                                                                            <  2:522802/250‑1 (MQ=255)

                                       gACACGCACTGATGAGATGAACGCTAAGTATCACGCGCCGGAGATTGCTTTCTCTGCGACGTACCTGAGCGCAATGTTGCGCCAGGAGATGTATAACAGTTATGGCGAAAGAGCCTATGAAGACGGTTATCGCATTTA‑ACAAACCCTCAACCGCCACGTGCAGCAGGCCGCGCAGCTGGCTGTACGTAATAACGTGCTGGCCTAGGGCATGCGCCCCGGCTATCGCGGCCCGGCAAAtgggatggggta                                                                                                                                                                                                           >  2:380080/1‑239 (MQ=255)

                                        acacGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGCAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAgg                                                                                                                                                                                                        >  1:121486/1‑250 (MQ=255)

                                            gCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGc                                                                                                                                                                                                    <  1:498469/251‑1 (MQ=255)

                                             cACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCg                                                                                                                                                                                                   >  1:413863/1‑251 (MQ=255)

                                              aCTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGa                                                                                                                                                                                                  <  1:189886/251‑1 (MQ=255)

                                                 gAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTc                                                                                                                                                                                               >  1:142561/1‑251 (MQ=255)

                                                  aGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACATGAGCGCAATGGCGCGCCAGGAGATGTATAACCGTTATGGCGAAAGCGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAACGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGGGGGAAGTGGGCGAGTCg                                                                                                                                                                                              >  2:564278/1‑251 (MQ=255)

                                                   ggCGATTAACGCTAACTATCACGCGCCGGCGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGCGATGTATAACCGTTATGGCGAAAGGGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGctcgtcgg                                                                                                                                                                                             >  1:489795/1‑244 (MQ=255)

                                                     cGATTAACGCTAAGTATCCCGAGACGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGCAATGGTGCGCCAGGAGCTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCCTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATACCGTGCTGGACTAGGAAATGCGCCACGGCTCTCGCGGCACGGCAAATGTGCTGTGGAAAGTGGGCGAGTAGGCg                                                                                                                                                                                           >  2:536688/1‑251 (MQ=255)

                                                      gATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCATGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGTCCCGGCAAATGAGCTGTGGAAAGTGGGCGAGTCGGCGt                                                                                                                                                                                          >  1:4434/1‑251 (MQ=255)

                                                          aaCGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTgggg                                                                                                                                                                                      >  1:9266/1‑250 (MQ=255)

                                                            cGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTgg                                                                                                                                                                                        <  1:128266/247‑1 (MQ=255)

                                                            cGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTgg                                                                                                                                                                                        >  2:128266/1‑247 (MQ=255)

                                                              cTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaac                                                                                                                                                                                  >  2:66370/1‑251 (MQ=255)

                                                                 aCTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaacaa                                                                                                                                                                                >  2:115957/1‑250 (MQ=255)

                                                                  cTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACATGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATaacaac                                                                                                                                                                               >  1:575377/1‑250 (MQ=255)

                                                                   tATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGGGCAGCAGGCCGCGCAGCAGGCGGTACGTACTAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCCGTGGAAGTTGGGCGAGTCGGCGTGGGATAaccaca                                                                                                                                                                              >  2:2839/1‑250 (MQ=255)

                                                                    aTCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAg                                                                                                                                                                            <  1:160251/251‑1 (MQ=255)

                                                                      cACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAacaaca                                                                                                                                                                              >  2:141805/1‑247 (MQ=255)

                                                                      cACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAacaaca                                                                                                                                                                              >  1:355676/1‑247 (MQ=255)

                                                                      cACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGa                                                                                                                                                                           <  1:508764/250‑1 (MQ=255)

                                                                         gcgcCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAACGTGGGCGAGTCGGCGTGGGATAACAACAAGATTAc                                                                                                                                                                       >  1:39461/1‑251 (MQ=255)

                                                                              ggAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATa                                                                                                                                                                  <  2:173171/251‑1 (MQ=255)

                                                                                     gcgttCTCTGCTCCGTACCTGAACGAAATGGTGCGCCAGGAGATGTATACCCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCCAAGTGCAGCCGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCACATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGa                                                                                                                                                            >  2:80982/4‑250 (MQ=255)

                                                                                                 ccGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAAc                                                                                                                                               <  1:442891/251‑1 (MQ=255)

                                                                                                   gTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCt                                                                                                                                             >  1:7512/1‑251 (MQ=255)

                                                                                                     cccggAGCGAAATGGTGCGCCAGGAGATGTATATCCCGTAGGGCGAAAGTTCCTATGAAGACAGTTATCGCCTTTACACCA‑CCATCCCCCTCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCCCGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCTAACCTAt                                                                                                                                           <  2:23039/247‑1 (MQ=255)

                                                                                                         gAGCGAAATGGTGCTCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACCCCA‑CCATCTCCCGCAAAGTGCAGCATGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCGATCGCGGCCCGGCAAATGTGCTGTGGAAAGGGGGCGAGTCGGCGTGAGATAACAACAAGATTACCGATCCGCTGACGGCACTGCCAGCCTATGGt                                                                                                                                        <  2:366152/250‑1 (MQ=255)

                                                                                                         gAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGGGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATgg                                                                                                                                         <  2:481829/249‑1 (MQ=255)

                                                                                                             gacatggctagacAGGAGATGTATAACAGTTATGGCGAAAGGGCAAATGAAGACGGTTATCGCTTTTACACCA‑GCATCACCCGCAAAGTGCAGAAGGCCGAGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGTCATGCGCCCCGGATATCGCGGCCCGGACAATGGGCTGAGGAAAGTGGGCGAATCGGCGGGGGATTACAACAAGATTGACGATACCCTGCAGGCGAAGCCAACCTATGGTCCgct                                                                                                                                   >  2:486643/13‑251 (MQ=255)

                                                                                                             gAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGACACTGCCTATGAAGACGGTTATCGCACTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCCAATGTGCTGTGGAACGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCgct                                                                                                                                   >  1:379914/1‑251 (MQ=255)

                                                                                                              aaaTGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTGCGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCgctg                                                                                                                                  >  2:208753/1‑251 (MQ=255)

                                                                                                                aTGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAAAAACAAGATAACCGATACGCTGAAGGCGCTGCCAACCTATGGTCAgctgct                                                                                                                                >  2:44968/1‑251 (MQ=255)

                                                                                                                     gcgcCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGctgcctg                                                                                                                           <  1:144987/251‑1 (MQ=255)

                                                                                                                       gcCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATATCGTGCTGGACTAGGACATTCGCCACGGCTATCGCTGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATa                                                                                                                                                                  <  1:379700/210‑1 (MQ=255)

                                                                                                                         cAGGAGATGTATAACCGTTATGGCGTACCTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCAACCGCCAAGAGCAGCAGGCCGCGCAGCAGGCGGTCCGTAATAACGTGCTGGACTCGGACATGAGCCACGGCTATCGCGGCCCGGCAAATGTGATGTGGCAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGACGc                                                                                                                       >  2:589247/1‑251 (MQ=255)

                                                                                                                              gaTGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTc                                                                                                                   >  2:247874/1‑250 (MQ=255)

                                                                                                                                   ataACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAgc                                                                                                             <  1:224619/251‑1 (MQ=255)

                                                                                                                                          ttATGGCGAAAGTGCCAACGCAGACGCTTATCACATTTCCACTA‑CCATCACCCGCAAAGTGCAGCAGGCCGAGCAGGAGGCGGTACGTAATCACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGCGCTGTGGAACGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCCATc                                                                                                      >  2:58383/1‑251 (MQ=255)

                                                                                                                                                    aGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCAACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGc                                                                                            >  1:37701/1‑251 (MQ=255)

                                                                                                                                                     gTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGTCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAGGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCg                                                                                           <  1:237536/251‑1 (MQ=255)

                                                                                                                                                     gTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCg                                                                                           >  1:66098/1‑251 (MQ=255)

                                                                                                                                                       gCCTATGAAGACGGTTATCGCATTTACACCC‑CCATCACCCGCAAAGGGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCGAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAGCAAGAAGATTGCCGAGACGCTGAAGGCGCTGCCAGCCTAGGGTCCGCTGCTGCCTGCGGCAGTCACCTGCGCCAATCCTCAGCTAGCGa                                                                                          <  2:533943/250‑1 (MQ=255)

                                                                                                                                                       gCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGAc                                                                                         <  1:564278/251‑1 (MQ=255)

                                                                                                                                                           aTGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTAACCAGCGCCAATCCTCCGCAAGCGACGGCg                                                                                     >  2:16773/1‑251 (MQ=255)

                                                                                                                                                            tGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGc                                                                                                                                                                                                    >  2:455870/1‑139 (MQ=255)

                                                                                                                                                            tGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGc                                                                                                                                                                                                    <  1:455870/139‑1 (MQ=255)

                                                                                                                                                             gAAGACGATTATCGCATTTACACCA‑CCATCCCCCGCCAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGAt                                                                                   >  2:377079/1‑251 (MQ=255)

                                                                                                                                                             aaaGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGAt                                                                                   <  2:538927/250‑1 (MQ=255)

                                                                                                                                                                   ggTTATCCAATTTACACCA‑CCATCACCCGCACAGTGAAGCAGGCCGCGCAGCAGGCGGTACGCAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGc                                                                             >  2:220666/1‑251 (MQ=255)

                                                                                                                                                                       aTCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGAc                                                                         >  2:438426/1‑251 (MQ=255)

                                                                                                                                                                         cGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACgg                                                                       <  1:262831/251‑1 (MQ=255)

                                                                                                                                                                          gCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACggg                                                                      >  1:130456/1‑251 (MQ=255)

                                                                                                                                                                           cATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGt                                                                     >  1:461596/1‑251 (MQ=255)

                                                                                                                                                                           cATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGt                                                                     <  1:295594/251‑1 (MQ=255)

                                                                                                                                                                             tttACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGAGGTAAGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCAGGCAAATAAGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCg                                                                   >  1:551360/1‑251 (MQ=255)

                                                                                                                                                                                acacCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCTCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGAc                                                                 <  1:237935/250‑1 (MQ=255)

                                                                                                                                                                                   cca‑ccaTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTc                                                             >  1:522164/1‑251 (MQ=255)

                                                                                                                                                                                         agcACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCAtt                                                        <  2:28635/249‑1 (MQ=255)

                                                                                                                                                                                         aTCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGGAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCATATGTGCTGTGGAAAGAGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCagt                                                        >  1:545421/1‑249 (MQ=255)

                                                                                                                                                                                               cGCAAAGTGCAGCAGGCCGCGCAGCCGGCGGTCCGTAATCACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTCCCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCCTTGGGTAt                                                  >  2:296766/1‑251 (MQ=255)

                                                                                                                                                                                               cGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCCATCCTCAGCAAGCGACGGCGATGCTGGCGGGAGGGTCGACCGGCGCCTTgcgg                                                    >  2:261676/1‑246 (MQ=255)

                                                                                                                                                                                                gCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGGCTTGAGTATg                                                 >  2:373397/1‑251 (MQ=255)

                                                                                                                                                                                                   aaGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGGGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGaa                                              <  1:387696/251‑1 (MQ=255)

                                                                                                                                                                                                     gTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAgg                                            >  2:187163/1‑251 (MQ=255)

                                                                                                                                                                                                        cagcagGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGt                                         >  1:508611/1‑251 (MQ=255)

                                                                                                                                                                                                              gCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTg                                   >  1:22622/1‑251 (MQ=255)

                                                                                                                                                                                                               ccGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTgg                                  <  1:115957/251‑1 (MQ=255)

                                                                                                                                                                                                                cgcgCAGCAGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGCGGGATAACAACCAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCAGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCACGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTggg                                 >  1:243851/1‑251 (MQ=255)

                                                                                                                                                                                                                   gcagcaGGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGGGCATGGAAGGCGTTCGCTGGgcgc                              >  2:447566/1‑251 (MQ=255)

                                                                                                                                                                                                                     agcagGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTTCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGTTTCGCCCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGt                            <  1:537725/251‑1 (MQ=255)

                                                                                                                                                                                                                        agGCGGTACGTAATAACGTGCTGGACTAGGACATGCGCCACCGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGCCGGAGTGGGCTCACAACAAGATTACAGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCt                         >  1:38586/1‑251 (MQ=255)

                                                                                                                                                                                                                                cgtgaaaaCGTGCTGGACTCGGACATGCGCCACGGCTACCGCAGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATTGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCTACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCTCGTCCTTACCGtgc                 <  2:419000/245‑3 (MQ=255)

                                                                                                                                                                                                                                 gTAATAACGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCg                >  1:436708/1‑251 (MQ=255)

                                                                                                                                                                                                                                  taataaCGTGCTGGCCTAGGAACTGCGCCACGGCTATCGCGGACCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGCGTATGGAAGGCGTTCGCTGGGCGCGGCCTTACCGTCCgg               >  2:237298/1‑251 (MQ=255)

                                                                                                                                                                                                                                   aataaCGTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCgg               <  1:472950/250‑1 (MQ=255)

                                                                                                                                                                                                                                         gTGCTGGACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTc         >  1:528234/1‑250 (MQ=255)

                                                                                                                                                                                                                                           gCTGGACTAGGAAATGCGCCACGGCTCTCGCGGCCCGGAAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTGCGGATACTCAg       <  2:355676/250‑1 (MQ=255)

                                                                                                                                                                                                                                              ggACTAGGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAACGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGGAGGAGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCa     >  2:460846/1‑249 (MQ=255)

                                                                                                                                                                                                                                               gACTAGGACATCCGCCACGGCTATCGAGGCCAGGCAAATGTGCTGTGGCAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTCAGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCCGTTACTCAGCAAgg  >  2:97943/1‑251 (MQ=255)

GCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCA‑CCATCACCCGCAAAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCAAATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCAACCTATGGTCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGCAAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCATTGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAAGG  >  NC_000913/3523557‑3524045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`