Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,116	C→T	P21L (CCT→CTT)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,116	0	C	T	100.0%	222.7 / NA	62	P21L (CCT→CTT)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/30);  total (32/30)

GAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTAC  >  NC_000913/497873‑498360                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     gAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGc                                                                                                                                                                                                                                                <  2:594500/250‑1 (MQ=255)      gAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTaaa                                                                                                                                                                                                                                          <  1:22075/251‑1 (MQ=255)         cAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACg                                                                                                                                                                                                                                       <  2:10991/251‑1 (MQ=255)          aGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGc                                                                                                                                                                                                                                      >  1:152597/1‑251 (MQ=255)                gCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTg                                                                                                                                                                                                                                <  1:484982/251‑1 (MQ=255)                         aaCTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAATCCTCAATCTGCGCTATTCCTTGTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGTCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAACGGTAGAACGCTATCTGAAACAAttt                                                                                                                                                                                                                       <  1:484569/251‑1 (MQ=255)                          aCTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTCCAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTGTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAAtttt                                                                                                                                                                                                                      <  1:389391/251‑1 (MQ=255)                              aTCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTaa                                                                                                                                                                                                                   <  1:153694/250‑1 (MQ=255)                                       ctcAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGAcg                                                                                                                                                                                                         >  2:213964/1‑251 (MQ=255)                                        tcAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAg                                                                                                                                                                                                            <  2:137738/247‑1 (MQ=255)                                        tcAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAg                                                                                                                                                                                                            >  1:137738/1‑247 (MQ=255)                                           ggCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTg                                                                                                                                                                                                     >  1:208437/1‑251 (MQ=255)                                             ccGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTGTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGt                                                                                                                                                                                                   <  2:208437/251‑1 (MQ=255)                                                  tatTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTCTCTGAAACACTTTTTAAGCGACAGCCGCGGGGTTGAt                                                                                                                                                                                               >  2:151910/1‑250 (MQ=255)                                                    ttcattcGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATAcc                                                                                                                                                                                            >  1:4818/1‑251 (MQ=255)                                                         tcGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCGGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCAc                                                                                                                                                                                        <  1:585951/250‑1 (MQ=255)                                                            gCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGAACTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGAAAACCTGGGTACGCCCGATGCCCCCACACTTGa                                                                                                                                                                                                                                                   >  1:249329/1‑188 (MQ=255)                                                            gCCTTTTACAAAAACCTCAATCTGCGCTATGCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGa                                                                                                                                                                                                                                                   <  2:249329/188‑1 (MQ=255)                                                             ccTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTAAGCCCGATGCCCCCACACTTGAAGCGGGAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACgggtg                                                                                                                                                                                   >  2:202717/1‑248 (MQ=255)                                                              cTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGgttgt                                                                                                                                                                                  >  1:203549/1‑251 (MQ=255)                                                                   aCAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTAtgg                                                                                                                                                                             >  1:181474/1‑251 (MQ=255)                                                                             cAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGGTGATACCTCACGGGTGTTATGGTGGCCATtgc                                                                                                                                                                   >  1:14699/1‑251 (MQ=255)                                                                                  tGCGCTATTCCTTTTTCTGATTTGACATCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGATGAATCGATAAGAGGCGGTAATGCGACAGACTAAAAACGGTATCCTGCTGGCAAATCTGGGTACGCCCGGAGCCCCCCCACTTGACGCGGTAAAAGGCTATCTTAAAAAATTTTTAAGCGAAAGACGCGAGGTTGAGACCTACCGGTTGCTTTTGTGGCCATtgttgggg                                                                                                                                                              >  2:272666/1‑245 (MQ=255)                                                                                        aTTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCgcggcg                                                                                                                                                          <  1:95211/249‑1 (MQ=255)                                                                                             tttttCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCTCGGCGTGAtttt                                                                                                                                                   >  2:318363/1‑251 (MQ=255)                                                                                              ttttCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGAttt                                                                                                                                                    <  1:373748/249‑1 (MQ=255)                                                                                                  cTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGc                                                                                                                                              >  2:66533/1‑251 (MQ=255)                                                                                                     aTTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTgc                                                                                                                                           >  1:536476/1‑251 (MQ=255)                                                                                                        tGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATGTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTTATGTTGCCGCTgcgc                                                                                                                                         <  2:379617/250‑1 (MQ=255)                                                                                                           ccTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTcgc                                                                                                                                     >  1:421607/1‑251 (MQ=255)                                                                                                              ctcACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATAcc                                                                                                                                                                                            >  1:155051/1‑193 (MQ=255)                                                                                                              ctcACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATAcc                                                                                                                                                                                            <  2:155051/193‑1 (MQ=255)                                                                                                                                tcttcCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCCAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATg                                                                                                                >  1:431281/1‑251 (MQ=255)                                                                                                                                   tcCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGGATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACTCTATCTGAAACAATTTTTAAGCGACAGACGCGTGTTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGcc                                                                                                              <  2:536476/250‑1 (MQ=255)                                                                                                                                       cACTTTTCCGCTCCAATTATCCACAAGTTGAATCGATCAGCGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCCGCGCTCGCCGCGTGGGGCGAAGCTGTATGCCtctgt                                                                                                         >  2:304230/1‑251 (MQ=255)                                                                                                                                          ttttCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGAGGTAATTCGTCAGACTAAAACCGGGATCCTGCTGTCCAACCTGGGTACGCTCGTCGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGGGGTTGACACCTCACGGTTGTTATTGTGGCCCTTGCTGCGCGGCGTGCTTTTGCCGCTGGGCTTGCCGCGTTTGGCGGAGCTGTATTCCtctgtatg                                                                                                      >  1:291865/1‑248 (MQ=255)                                                                                                                                          ttttCAGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCtctgtctg                                                                                                      >  1:167729/1‑251 (MQ=255)                                                                                                                                                gCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGCTACCTCACGGTTGTTTTGGTGGACATTTCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGGAACTGAATGCCTCTGTCtggttggg                                                                                                >  2:26065/1‑250 (MQ=255)                                                                                                                                                           caacaaGTTGAATCGATAAGAGGCTGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCTCGTTTGTTATGGTGGCCATTGCTGCTCGGCGTGATTTTGCCGCTGCGCTCGCCTCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTcgc                                                                                     <  1:272666/251‑1 (MQ=255)                                                                                                                                                             acaaGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTcgccg                                                                                   <  1:315539/251‑1 (MQ=255)                                                                                                                                                                 tttGAATCGATAAGAGGCGGTAATGCGTCAAACTAAAACCGGTATCCTGCTGGCAAACCTGGGTAAGCCCGCTGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGCCAGACGCGTGGTTGATACCTCACGGTTGTTATGGGGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGGGGCGAAGCTGTATGCCCCTGTCTGGATGGAAGGTGGCTcgccgcgtc                                                                               >  2:361420/2‑248 (MQ=255)                                                                                                                                                                      aTCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGtt                                                                          <  2:258483/251‑1 (MQ=255)                                                                                                                                                                         gATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTAc                                                                       <  1:66533/251‑1 (MQ=255)                                                                                                                                                                             agagGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGGAGGTGGCTCGCCGCTGATGGTTTACAgcc                                                                   <  2:450513/251‑1 (MQ=255)                                                                                                                                                                                 gCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGTTGGAAGGTGGCTCGCCGCTGATGGTTTACAgccgcc                                                                >  1:348249/1‑250 (MQ=255)                                                                                                                                                                                 gCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAgccgcc                                                                >  1:494116/1‑250 (MQ=255)                                                                                                                                                                                  cgggaaTGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAg                                                              <  2:447661/247‑1 (MQ=255)                                                                                                                                                                                                   aaaaCCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGTCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTgg                                              <  1:304230/250‑1 (MQ=255)                                                                                                                                                                                                    aaaCCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCGGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGca                                            <  1:403485/251‑1 (MQ=255)                                                                                                                                                                                                     aaCCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGcac                                           >  1:54540/1‑251 (MQ=255)                                                                                                                                                                                                        cGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAAc                                        >  2:296700/1‑251 (MQ=255)                                                                                                                                                                                                          gTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGGGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGt                                      >  2:464310/1‑251 (MQ=255)                                                                                                                                                                                                                    ggCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCtggatgg                                                                                                 >  1:194821/1‑182 (MQ=255)                                                                                                                                                                                                                    ggCAAACCTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCtggatgg                                                                                                 <  2:194821/182‑1 (MQ=255)                                                                                                                                                                                                                        atccTGTGTACGCCCGATGCCCCCACACTGGAAGCGGTAAAACGCTATCGGAAACAATTTTTAAGCGAAAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCGGCGCGGCGTGATTGTGTCGCTGCGCTCTCGGCGTGGGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGTTGGCGCGCCGCTGAGTGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGcc                        <  2:553910/249‑1 (MQ=255)                                                                                                                                                                                                                          ccTGGGTACGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCg                      <  1:252758/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCGCCCGATGCCCCCACACTTGAAGCGGTAAACCGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATCCCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCt               <  2:431281/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGCCCGATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTTATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGTTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTg              <  1:389810/251‑1 (MQ=255)                                                                                                                                                                                                                                       gATGCCCCCACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGCCAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCAATTGCTGCGCGGCGTGCTTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAAt         >  1:387034/1‑251 (MQ=255)                                                                                                                                                                                                                                             cccACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCt    >  1:513623/1‑250 (MQ=255)                                                                                                                                                                                                                                              ccACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTAc  <  1:296700/251‑1 (MQ=255)                                                                                                                                                                                                                                              ccACACTTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTAc  >  1:85905/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     GAGGGGAACAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTAC  >  NC_000913/497873‑498360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑