Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,242,968	G→T	intergenic (‑1/+16)	yeiB ← / ← folE	DUF418 domain‑containing protein YeiB/GTP cyclohydrolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,242,968	0	G	T	100.0%	165.9 / NA	44	intergenic (‑1/+16)	yeiB/folE	DUF418 domain‑containing protein YeiB/GTP cyclohydrolase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/19);  total (25/19)

TTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGG  >  NC_000913/2242746‑2243208                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 gcttcggtatctctgaaggcatggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccgttgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttggtttcttgcaatggtgtctctctctagcccgcataaTCGAGCGTTACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGtgatga                                                                                                                                                                                                                      <  1:380999‑M2/52‑1 (MQ=255)             ctgaaggcatggtgatggctgccggtcctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAgcg                                                                                                                                                                                                             <  2:527800‑M2/61‑1 (MQ=255)                                       cctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGAc                                                                                                                                                                                 <  1:27488‑M2/89‑1 (MQ=255)                                       cctggcgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGAc                                                                                                                                                                                 <  2:333252‑M2/89‑1 (MQ=255)                                            cgggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGCCGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGAtt                                                                                                                                                                           >  1:341215‑M2/157‑251 (MQ=255)                                             gggaaagatattttcctgctaagcccggatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGAttt                                                                                                                                                                          >  1:306049‑M2/156‑251 (MQ=255)                                                                        gatgccggtgctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGt                                                                                                                                               >  1:323773‑M2/129‑251 (MQ=255)                                                                                  ctaaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTg                                                                                                                                     >  1:317552‑M2/119‑251 (MQ=255)                                                                                   taaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattactaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTgg                                                                                                                                    >  1:76316‑M2/118‑251 (MQ=255)                                                                                    aaaccgggtcatcaggtgaaataatcccccttcaaggcgctgcttcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGt                                                                                                                                   >  1:378390‑M2/117‑251 (MQ=255)                                                                                              atcaggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCgg                                                                                                                         <  2:310444‑M2/145‑1 (MQ=255)                                                                                                 aggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgc                                                                                                                                                                                             <  1:248101‑M2/77‑1 (MQ=255)                                                                                                 aggtgaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGgc                                                                                                                                                                                             >  2:248101‑M2/104‑180 (MQ=255)                                                                                                     gaaataatcccccttcaaggcgctgcatcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCa                                                                                                                   <  2:154554‑M2/151‑1 (MQ=255)                                                                                                             cccccttcaaggcgctgcatcgacagcgccttttctatataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTc                                                                                                          <  2:197283‑M2/160‑1 (MQ=255)                                                                                                                              catcgacatctccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCCCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGTCGCGTATTCTGACTGGTTTTGAACTATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCGTTCACGCAGTAATGCACcgc                                                                                         <  1:410151‑M2/177‑1 (MQ=255)                                                                                                                              catcgacagcgccttttctttataaattcctaaagttgttttcttgcgattttgtcgctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTGTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAGCAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTGGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACcgc                                                                                         <  1:271032‑M2/177‑1 (MQ=255)                                                                                                                                   acagcgccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGtcg                                                                                     >  1:576189‑M2/70‑250 (MQ=255)                                                                                                                                        gccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGtcgatcg                                                                                 >  1:444480‑M2/65‑249 (MQ=255)                                                                                                                                         ccttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGtcgatcg                                                                                 >  1:531637‑M2/64‑248 (MQ=255)                                                                                                                                          cttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCAACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGAc                                                                             >  1:530261‑M2/63‑251 (MQ=255)                                                                                                                                           ttttctttataaattcctaaagttgttttcttgcgattttgtctctctctaaaccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCCCAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGAc                                                                             >  1:571663‑M2/62‑250 (MQ=255)                                                                                                                                                 ttataaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCa                                                                        <  1:288669‑M2/194‑1 (MQ=255)                                                                                                                                                     aaattcctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTAt                                                                  <  2:227267‑M2/200‑1 (MQ=255)                                                                                                                                                           ctaaagttgttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTattggggc                                                            >  2:473691‑M2/46‑248 (MQ=255)                                                                                                                                                                   gttttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCagcagc                                                    <  1:554343‑M2/214‑1 (MQ=255)                                                                                                                                                                      ttcttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGt                                                  <  2:323773‑M2/216‑1 (MQ=255)                                                                                                                                                                        cttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTg                                               >  1:389060‑M2/33‑251 (MQ=255)                                                                                                                                                                         ttgcgattttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGt                                              >  1:419980‑M2/32‑251 (MQ=255)                                                                                                                                                                              attttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGCTGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGAAAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAgcgc                                         >  2:561002‑M2/27‑251 (MQ=255)                                                                                                                                                                                 ttgtctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAAt                                      >  1:289171‑M2/24‑251 (MQ=255)                                                                                                                                                                                     ctctctctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCCCGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTttgtagcgacata                                     >  2:540015‑M2/20‑243 (MQ=255)                                                                                                                                                                                           ctaacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGGCGATCGAGACAGACACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAAtt                               <  1:336884‑M2/235‑1 (MQ=255)                                                                                                                                                                                            taacccgcataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTtgct                           >  1:140531‑M2/13‑251 (MQ=255)                                                                                                                                                                                                    ataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGCCGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGCTTTGAACAATCCACCAAGCGAGGTCGTTGTCGTGGCCCTGGTTGCATCGCGGATTCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACCGCCACGTTATTGGTGCCCACCAGCGTTTGTAGCGCCCTACGAATTTGCTGTgtccg                    >  2:303‑M2/5‑248 (MQ=255)                                                                                                                                                                                                    ataaTCGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCACTAAGAATTTGCTGTGTc                      >  2:400567‑M2/5‑248 (MQ=255)                                                                                                                                                                                                      aaTCGCGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCCCAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACg                 >  2:586517/1‑251 (MQ=255)                                                                                                                                                                                                         cGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGtt               <  1:479939/250‑1 (MQ=255)                                                                                                                                                                                                         cGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTc              <  2:191923/251‑1 (MQ=255)                                                                                                                                                                                                         cGAGCGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTc              >  2:412387/1‑251 (MQ=255)                                                                                                                                                                                                             cGTGACGTTGCGCTCCATTGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGCAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATCAGAATTTGCTGTGTCAGACGTTCCTGc          >  2:534673/1‑251 (MQ=255)                                                                                                                                                                                                                 aCGTTGCGCTCCATTGTTCCTGCCGTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTTTGTCAGACGTTCCTGCACCt      <  1:443883/251‑1 (MQ=255)                                                                                                                                                                                                                   gTTGCGCTACATTGTTCCTGCCTTTTACTCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGTGTCAGACGTTCCTGCACCTGc    >  2:224682/1‑251 (MQ=255)                                                                                                                                                                                                                      gcgcTCCATTGTTCCTGCCTTTTAATCAGTGGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTAGTCGGACTGGATTTGAACAATCCACCAAGAGATGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTTCTGTGTCAGACGTTCCTGCACCTGCgg  <  2:389060/250‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 TTGCAGGCCCGCACCAAACAGTAGCGCAAAAAGCGTGAGGAATTTCACCTGGCCGATGAGATCAAGAAATGCCCAAGTCCATGCATCCCGCGGCGTAATAGCACCGTACCAGGCGGGATTGAGATAAGCCGCCTTTGGTAGCCCAAAGGCGCTGATGTTTAATAGCAGGATCCCCAGAATGGCGACGCCGCGAACAAAATCGAGCGTGACGTTGCGCTCCATGGTTCCTGCCTTTTAATCAGTTGTGATGACGCACAGCGCGCAGAAACTCGTGGCGCGTATTCTGACTGGATTTGAACAATCCACCAAGAGAGGTCGTTGTCGTGGCACTGGTTGCATCGCGGATGCCACGCGCCTTCACGCAGTAATGCACCGCGTCGATCGAGACAGCCACGTTATTGGTGCCCAGCAGCGTTTGTAGCGCAATAAGAATTTGCTGCGTCAGACGTTCCTGCACCTGCGG  >  NC_000913/2242746‑2243208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑