Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	367.5 / NA	97	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (51/46);  total (51/46)

CTTTTACCATCAACGTGTTAGCAACGTTGAGATTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGT  >  NC_000913/3560210‑3560692                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               cttTTACCATCAACGTGTTAGCAACGTTGAGATTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTCTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGcgcagg                                                                                                                                                                                                                                            >  1:120772/1‑246 (MQ=255)  ttttACCATCAACGTGTTAGCAACGTTGAGATTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGcgcagg                                                                                                                                                                                                                                            >  1:471051/1‑245 (MQ=255)  ttttACCATCAACGTGTTAGCAACGTTGAGATTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGcgcagg                                                                                                                                                                                                                                            >  1:36345/1‑245 (MQ=255)    ttACCGTCATCGTGTTAGAAACGTTGA‑AATGTGTGGTG‑CAATTGCACAATTTG‑CTGTTATTGCGCGGTGCGTGCCCTCCCTCTCCCGGCGGGGCCCCGATCTCGAAACACAGCGTCGACCCTTGGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGAGGCCC‑GCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCCTGCCACGGCTTGTTAACC‑GAACTGG‑AAGG‑CGGCGCCGCACCGCCATGATGGCGCAGGAt                                                                                                                                                                                                                                          <  2:356344/250‑1 (MQ=255)    ttACCATCAACGTGTTAGCAACGGTGAGATTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATc                                                                                                                                                                                                                                         >  1:126706/1‑251 (MQ=255)       ccaacAACTTGTAAGCAACGTTAGGATTGT‑TGGTGAAAAT‑GCGCAAATTG‑CTGTGTTTGACCAGTGTGTGCGTTACCGCTTCCGTGGTGGGGCCGAGATCAAAAAAGAGCTTCGACCAATTGGGG‑ATTTGCTCCT‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGGGTGTTAACC‑GACCGGT‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAgg                                                                                                                                                                                                                                      <  1:214607/247‑1 (MQ=255)           cAACGTGTTAGCAACGTTGAGATTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGtttt                                                                                                                                                                                                                                  >  1:297760/1‑251 (MQ=255)                     gCAACGTTGAGATTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                          >  1:359821/1‑249 (MQ=255)                      aaaCGTTGAGATTGT‑GGGTGACAAT‑GCGCAATTTG‑TTGTGATTGAGCAGGGCGGCCGCTCCCGCTTCCGGCGGGGGGCCGATATGAATAAACAGCGTCGAGCCATGGGGG‑ATTTGCTCCG‑CCTCTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGc                                                                                                                                                                                                                       <  1:18090/250‑1 (MQ=255)                            tGAGATTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCAGCCCGCTCATTg                                                                                                                                                                                                                 >  2:549263/1‑251 (MQ=255)                             gagaTTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCGTGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                                 >  1:120110/1‑250 (MQ=255)                             gagaTTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTgg                                                                                                                                                                                                                >  2:458681/1‑250 (MQ=255)                                aTTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGt                                                                                                                                                                                                             >  2:116390/1‑251 (MQ=255)                                  ggt‑tgGTAAAAAT‑GCGCAAATTG‑TTGTGATTGAGCATGGCGGGCCCTACCGCTCCCGGCGGGGGCCCGATATGAATAAACAGCGCCGGGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg                                                                                                                                                                                                           <  1:511959/250‑1 (MQ=255)                                   gt‑tgGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                          >  1:484286/1‑251 (MQ=255)                                      tgGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                                                                                                                                        >  2:28975/1‑251 (MQ=255)                                          gACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa                                                                                                                                                                                                     >  2:273303/1‑250 (MQ=255)                                            aaaT‑GCG‑AAATTGGCTGTGATTGAGCAGGTCGTGCGCTACCGCTTCGGGCGTGGTGCCGATATCGATAAACAGCGTCAAGCCTTTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                                                                                                                  <  1:206594/250‑1 (MQ=255)                                               t‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc                                                                                                                                                                                               >  1:216216/1‑251 (MQ=255)                                                ggcgcAAATTG‑TGGTGATGGAGCGGTGCGTGCGCTACCGCTCCCGGCGGGGTCCCGATTTCGAAAAACAGCGTCGAGCCATGGGGG‑TTTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATAGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTc                                                                                                                                                                                               <  1:41604/250‑1 (MQ=255)                                                        atggttgggATTGAGCGGTGCGGGCGCTCCCGTTGCGGGGGTGGTGCAGATCTCGAAAAACGGCGGCGAGCCATTGGGG‑ATTGGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                                                                                                                        <  1:495850/243‑1 (MQ=255)                                                              gtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                                  >  2:457577/1‑251 (MQ=255)                                                                    gAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt                                                                                                                                                                            >  1:153914/1‑251 (MQ=255)                                                                      gCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑CTTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTCACC‑GAACTGG‑ACGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGCGAAATGc                                                                                                                                                                          >  2:125597/1‑251 (MQ=255)                                                                        aGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                                                                                                                                                                        >  1:430304/1‑251 (MQ=255)                                                                             gtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                                    >  1:29150/1‑250 (MQ=255)                                                                             gtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc                                                                                                                                                                    >  1:321650/1‑250 (MQ=255)                                                                                 gcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGTGCCGCCAGCTCATTGAGGTCGCGGCGAATAGACTGCGGGCTGAGGGAGAAATGCTCTACCAGctc                                                                                                                                                               >  2:2492/1‑251 (MQ=255)                                                                                  cTACCGCTTCCGGCGCGGTGCCGCTATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCCCGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCCCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGctct                                                                                                                                                              >  2:224168/1‑251 (MQ=255)                                                                                    aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                            >  1:186/1‑251 (MQ=255)                                                                                      cGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg                                                                                                                                                          >  2:468784/1‑251 (MQ=255)                                                                                         ttCCGGCGTGGTGCCGATACCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCCGGATCCGGTTTTGCTCCGCCAGCTCATTTAGGTCGCGGCGGATAGTCTGCGGGCTGACGgaga                                                                                                                                                                               >  2:287069/1‑227 (MQ=255)                                                                                            gggCGGGGTGCCGATATCGATAAACGGCGTCGGGCCTTTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                                    <  1:81035/250‑1 (MQ=255)                                                                                            cGGCGAGGCGCCGATATCGATAAACAGAGTCAAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGACGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCCGTCGGAGGAATGCTCTATCAGCTCTTCGGTACTg                                                                                                                                                     >  2:50813/1‑250 (MQ=255)                                                                                               cGTGGTGCAAATACCGTTAAACAGCGTCGAGCCTTGGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGGACTGACAt                                                                                                                                                 <  1:147824/251‑1 (MQ=255)                                                                                                         atatCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg                                                                                                                                       >  1:483574/1‑251 (MQ=255)                                                                                                          tatCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                                                                                                                                      >  2:325739/1‑251 (MQ=255)                                                                                                           atCGAAAAACAGGTTCGAGCCATGGGGA‑ATTTGCGCCG‑CCTCTTTGCGGGCGATGCGC‑TTTTTGTCTGCGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGAGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTTACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                                     <  1:528975/251‑1 (MQ=255)                                                                                                             gagataaccggctccggCCATTGGGT‑TTTTGCGCCGGCCTCTTTGCGGGGGATGCGC‑TCTTTTTCTGCGGTCTGGGTGGCCTGGCGTTCGTCCCACGGCGTGGTACCC‑GAACGGG‑AAGG‑CAGCCCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCATCTCTTCGGTTCTGGCATAACCCTGCTGtt                                                                                                                                     <  2:242953/234‑1 (MQ=255)                                                                                                                 aaaCAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                                >  1:500744/1‑250 (MQ=255)                                                                                                                       gcgtggaccATTGGGGGTTTTGCCCCG‑CCCCTTGGGGGGCGATGCGC‑TCTTTTTCTCCGGTCTGGGTGGCCTTGCGATCGTCCCACGGCGGGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                                                                                                                          <  2:297760/244‑1 (MQ=255)                                                                                                                        ggcGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGGGGCCTTGCTATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CCGCGCCGCACCGCCATGCTGGCGCAGGAACAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAACAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCTGTACTGACACAACCCTGCTGTTTAACCAGTTCGat                                                                                                                        >  2:106890/3‑251 (MQ=255)                                                                                                                           gAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GACCTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataat                                                                                                                     >  1:69717/1‑251 (MQ=255)                                                                                                                                cctgggggtattgtcccccccccTTTGCGGGCG‑TGCCCCTCTTTTTCTCCGGCCGGGGTGGCCTTGCGATCGGCCCACGGCGTGTTAACC‑GAACGGG‑AAGG‑CCGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAcc                                                                                                                  <  2:321650/229‑1 (MQ=255)                                                                                                                                  ggggggtttggccccg‑ccgCTTGGCGGGGGATGCGC‑TCTTTTTCCGCGGGCTGGGGGGCCTTGCGACGGTGCCAGGGCGTGTTAACA‑GAACGGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                               <  2:415315/238‑1 (MQ=255)                                                                                                                                  ggggggagtggCTCCG‑CCCCTTGGCGGGGGAGGCGC‑TCTTTTTCGTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GATGGGG‑AGGG‑CCGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                               <  2:178029/241‑1 (MQ=255)                                                                                                                                   gggg‑ATTTGCTCCG‑CCACTTTGCGGGCGCAGCGC‑TCTTTTTCTTCGGCCTGTGCGGCCTTCAGATCGTGCCACAGCGTGTTCACA‑GAACTGG‑AAGG‑CAGCGACGCCCCGCCCTGATGGCGCCGGAACAGGTTTTGCTCCGCCAGATCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCACCTCTTCGGTACTGACATAACCCTGCTGTTTAACAAGTTCGATAATCCCGTtgg                                                                                                             >  1:311359/1‑250 (MQ=255)                                                                                                                                    ggg‑ATTTGCTCCG‑CCACTTGGCGGGCGATGCGC‑TCTTTTTCTTCGGTCGGGGTGGCCTGGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg                                                                                                            <  1:445220/251‑1 (MQ=255)                                                                                                                                           tccTCCG‑CCCCTTTGCGGGCGATGCAC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTCCCACGGCGTGTAAACC‑GAACTGG‑AAGG‑CAGCGCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                       <  1:2492/248‑1 (MQ=255)                                                                                                                                            tctgccgcgatgttgggggCGATGCGC‑TCTTTTTCTGCGGTGTGGGGGCCCTTGGGATCGTGCCAGGGCGGGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGtt                                                                                                       <  2:201457/234‑1 (MQ=255)                                                                                                                                            gCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtg                                                                                                      <  1:116390/250‑1 (MQ=255)                                                                                                                                            cccccG‑CCACTTTGCGGGCGAGGCGC‑TCTTTTTCTTGGGCCTGGGTGGCCTGGCGATCTGGCCACGGCGTGTTAAC‑‑GAACGGGGAAGC‑CAGCGCCGCACCGCCAGGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCTATAATACCGTTGTGACGTtgt                                                                                                     <  2:306565/248‑1 (MQ=255)                                                                                                                                                cG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGTTGGCCTTGCGATCGGGCCACGGCGTGTTAACC‑GAACGGG‑AAGG‑CAGCGCCGCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGAGAATTCCGTTTTGTCGTTGtgttt                                                                                                 <  1:470558/251‑1 (MQ=255)                                                                                                                                                   ccACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGCTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt                                                                                               >  2:214169/1‑251 (MQ=255)                                                                                                                                                     aCTTTGGGGGCGATGCGC‑TCTTTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTgtttgttt                                                                                             <  1:108139/251‑1 (MQ=255)                                                                                                                                                       tttGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                                                            >  2:155446/1‑250 (MQ=255)                                                                                                                                                          gCGGGCGATGCGC‑TCTTTTTCTTCGTTCTGGGGGGCCTTGGGTCCGTGCCACGGCGTTTTAACC‑GACCGGG‑AAGG‑CAGCCCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAtt                                                                                         <  2:29150/250‑1 (MQ=255)                                                                                                                                                          gCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                        <  1:5627/251‑1 (MQ=255)                                                                                                                                                          gCGGGCGATGCCC‑TCTTTTTCTTCGGTCTGGGTGGACTTGCGATCGTGACAGGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGAAGCACCGCCATGATGGCGCAGGATCACGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGTGAATAGTCTGCGGGCTGACCGAGAAATGCTCGACCAGCTCTTCTGTACTGACATAAACCTGCTGTTTATACAGTTCGATACTACCGTTTTGCAGTTGTGTTTGTCTCAttt                                                                                        >  2:593052/1‑251 (MQ=255)                                                                                                                                                           ggggCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTta                                                                                       <  1:273303/250‑1 (MQ=255)                                                                                                                                                            gggCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCTTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtat                                                                                      <  1:196329/251‑1 (MQ=255)                                                                                                                                                             ggCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTtat                                                                                      >  1:164578/1‑250 (MQ=255)                                                                                                                                                                gATGCGC‑TCTTTTTCTTCGGTCAGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCAATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGACCTGACATAAGCCTGCTGTTTAACAAGTTCGATACTCCCGTTGGTACGTTGTGTTTGTTTAATTTCTAAAt                                                                                  >  2:24086/1‑251 (MQ=255)                                                                                                                                                                   gcgc‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCAATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGct                                                                                                                                                                >  1:286552/1‑170 (MQ=255)                                                                                                                                                                    cgc‑TCTTTTTCTTCTGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCAGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                              >  2:533387/1‑251 (MQ=255)                                                                                                                                                                    cgc‑TCTTTTTCTTCGTTCTGGGTGGCCTGGCGATCGGGCCACGGCGTGTTAACC‑AAACTGA‑AAGG‑CAGCCCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCt                                                                              <  1:518903/251‑1 (MQ=255)                                                                                                                                                                     cc‑tctTTTTCTTCGGTCGGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CGGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTCCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTg                                                                             <  1:28975/250‑1 (MQ=255)                                                                                                                                                                              tctccgggcggggTGGCCTGGCGACCGTGCCACGGCG‑GTTAACCCGAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattat                                                                      <  2:470992/240‑1 (MQ=255)                                                                                                                                                                                 tcggtcggGGTGGCCTTGCGATCGGGCCCCGGCGTGTTAACC‑GACCGGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattatt                                                                     <  1:134999/248‑1 (MQ=255)                                                                                                                                                                                 tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCAGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAATCATGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTATTTTATAACTCCCTGGAATTAttttt                                                                  >  2:19793/1‑250 (MQ=255)                                                                                                                                                                                  cGGTCGGGGGGGCTTTGCGATCGGGCCCCGGCGGGTAAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt‑c                                                                 <  2:492147/250‑1 (MQ=255)                                                                                                                                                                                   ggtcggggggcccTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                                   <  2:99623/109‑1 (MQ=255)                                                                                                                                                                                   ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGact                                                                                                                                                                                                   >  1:99623/1‑118 (MQ=255)                                                                                                                                                                                        gggTGGCCTTGGGAGCGGGCCGCGGGGTTTTAACC‑GAACGGG‑AGGG‑CACCGCCGCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGTAATTAtttt‑cgttt                                                             <  2:161793/248‑1 (MQ=255)                                                                                                                                                                                         ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTcgc                                                         >  2:16082/1‑251 (MQ=255)                                                                                                                                                                                         ggTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTcgc                                                         <  1:328777/251‑1 (MQ=255)                                                                                                                                                                                         ggTGGCCTTGCGATCGAGCCAGGGCGTGAAGATT‑GAACTGT‑AAGG‑CAGCGCCTAACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAGTGATGTCTCGGCGAATAGTCTGCGGGCTGACAGAGATATGCTCTACCAGCTCTTCGGTACTGACATGACCCTGCGGTTTAACCATTTCTATAATACCGTTGTGGTGATGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTcgc                                                         <  1:562564/251‑1 (MQ=255)                                                                                                                                                                                          gTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTcgcg                                                        >  1:198232/1‑251 (MQ=255)                                                                                                                                                                                           ggccctggagatgtggCCACGGCGTGTGAACC‑GAAC‑GG‑AAGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTTCGTTTTcgcg                                                        <  2:487932/236‑1 (MQ=255)                                                                                                                                                                                                 tGCGATCGTCCCACGGCGTGTTAACC‑GAACGGG‑AAGG‑CAGGGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTG‑CGTTTTCGCGCATTg                                                   <  1:458681/249‑1 (MQ=255)                                                                                                                                                                                                  gCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGc                                                >  2:418377/1‑251 (MQ=255)                                                                                                                                                                                                            cACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATcaaca                                       >  2:317191/1‑250 (MQ=255)                                                                                                                                                                                                                    gtTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCAt                               <  1:6392/250‑1 (MQ=255)                                                                                                                                                                                                                    gtTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc                              <  1:317191/251‑1 (MQ=255)                                                                                                                                                                                                                    gtTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATc                              <  1:458765/251‑1 (MQ=255)                                                                                                                                                                                                                        aCC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGCTAATACCGTTGTGACGTTGTGTTTGTTTCATTTATACATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                           >  1:40858/1‑250 (MQ=255)                                                                                                                                                                                                                        aCC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTa                          <  1:156807/251‑1 (MQ=255)                                                                                                                                                                                                                          c‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa                        <  1:587540/251‑1 (MQ=255)                                                                                                                                                                                                                              aCTGG‑AAGG‑CAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACACAAGCCATCGCTAAAccc                     >  2:177343/1‑251 (MQ=255)                                                                                                                                                                                                                                ggg‑AAGG‑CAGCCCCGCACCGCCAGGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAc                   <  2:198232/250‑1 (MQ=255)                                                                                                                                                                                                                                 gg‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACg                  >  2:217202/1‑251 (MQ=255)                                                                                                                                                                                                                                  g‑AAGG‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACgg                 >  2:299508/1‑251 (MQ=255)                                                                                                                                                                                                                                    aggg‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGc                <  2:483574/249‑1 (MQ=255)                                                                                                                                                                                                                                      gg‑CAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGTCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgttt                                                                                                 >  1:304375/1‑169 (MQ=255)                                                                                                                                                                                                                                          ggcgcCGCACCGCCATGATGGCGCAGGATCAGGTTTTGATCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc           <  1:224168/250‑1 (MQ=255)                                                                                                                                                                                                                                            cgccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCCGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg         >  2:327845/1‑251 (MQ=255)                                                                                                                                                                                                                                            cgccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt‑cgttt                                                             <  1:83396/199‑1 (MQ=255)                                                                                                                                                                                                                                            cgccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt‑cgttt                                                             >  2:83396/1‑199 (MQ=255)                                                                                                                                                                                                                                             gccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCgg        >  1:9526/1‑251 (MQ=255)                                                                                                                                                                                                                                                   ccGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAtgt  >  1:310993/1‑251 (MQ=255)                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               CTTTTACCATCAACGTGTTAGCAACGTTGAGATTGT‑TGGTGACAAT‑GCGCAAATTG‑CTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCG‑CCACTTTGCGGGCGATGCGC‑TCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACC‑GAACTGG‑AAGG‑CAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTT‑CGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGT  >  NC_000913/3560210‑3560692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑