Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,761	G→A	G25D (GGT→GAT)	mntP →	Mn(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,761	0	G	A	100.0%	120.3 / NA	36	G25D (GGT→GAT)	mntP	Mn(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/19);  total (17/19)

GGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCT  >  NC_000913/1905530‑1906007                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       ggCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAAcc                                                                                                                                                                                                                                      <  2:110650/250‑1 (MQ=255)               gAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGc                                                                                                                                                                                                                       <  2:367934/251‑1 (MQ=255)                aaTACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGTGGCATCAATCGGTAAAGATGCCCCCCTCCATAAACCGAGTTTTTCTGAAga                                                                                                                                                                                                                       >  2:461757/1‑249 (MQ=255)                   acgttCAGTCAGGCGAGTCCATATGCACATCAATCGCTATGCCTTCATGAACGCGAAAACCATCACTGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCAtt                                                                                                                                                                                                                    <  1:173208/247‑1 (MQ=255)                    cTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGc                                                                                                                                                                                                                  >  1:89439/1‑251 (MQ=255)                      ttCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGc                                                                                                                                                                                                                  <  1:388938/249‑1 (MQ=255)                          gtcagGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCg                                                                                                                                                                                                            <  2:89439/251‑1 (MQ=255)                             agGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGTGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTGGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCTCCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGcc                                                                                                                                                                                                         <  1:376323/251‑1 (MQ=255)                                 gagaCCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAATCATTGCGAACCGGCCTTAt                                                                                                                                                                                                     <  2:102172/251‑1 (MQ=255)                                    aCCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGCGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAtttt                                                                                                                                                                                                  >  1:381581/1‑251 (MQ=255)                                           gCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGTACATTGTCATTAATATCACTGCTACTTTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCATTCGGTAAAGATGCCACCCGCCATAGACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGc                                                                                                                                                                                            <  1:514988/250‑1 (MQ=255)                                           gCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGc                                                                                                                                                                                            <  1:79087/250‑1 (MQ=255)                                           gCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCGTATTTTTGGTGc                                                                                                                                                                                            <  2:267424/250‑1 (MQ=255)                                            cacaTCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCg                                                                                                                                                                                          <  1:122980/251‑1 (MQ=255)                                                       cTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCCTGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTAGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTg                                                                                                                                                                               >  1:522810/1‑251 (MQ=255)                                                        tATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGc                                                                                                                                                                                            >  2:433386/1‑237 (MQ=255)                                                        tATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGc                                                                                                                                                                                            <  1:433386/237‑1 (MQ=255)                                                                            aaCCATCACGGTTTGTTGTTCATGTATGTATGTGTGCTGGGGGCGATGATGTGGTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTAGGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCGCCCCTCCAGAAACCGAAATTTTCTGAAGCATTGCGATCCGGCCGGAGTGTTGGTGCCGGCGAAACCCTGACGTCGCTGATCGGCTGGGGa                                                                                                                                                          <  2:241439/251‑1 (MQ=255)                                                                                aTCACGGCTTGTTGTTCATGTATGCATATTTGCTGGGGGCGCTGATGTGTTTTATGGATACCCCGGTCAGGACCTTGTCATGACTATCACTGCTATTGTTCTTCTTGAGATTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGAAAAGAAGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGAGAACCGGCATTATTTGTGGTGCCGTCGACACACTGACGACGCTGATCGGCTGGGGAATgg                                                                                                                                                      >  1:228768/1‑251 (MQ=255)                                                                                     ggCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATg                                                                                                                                                 >  1:293591/1‑251 (MQ=255)                                                                                       cTTGTTGTTCATGTATGAATGTTTGTTGGGGGCCATGCTGTGCTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCCCCCTCCATAAACCGAAATTTTCTGAAGACTTGCGAACCGGCCTTATTTTTGGTGCCGTCGCAACCCTGACGCCCCTGATCGGCTGGGGCATCGGCATGtt                                                                                                                                               >  2:107730/1‑251 (MQ=255)                                                                                        ttgttgTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGtt                                                                                                                                               >  2:197046/1‑250 (MQ=255)                                                                                                     aTGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACAACGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGCATGTCGACGGATGCATTTGCTGCATCCATAGCTACAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGAGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTg                                                                                                                                 >  1:289256/1‑251 (MQ=255)                                                                                                      tGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGt                                                                                                                                >  1:472579/1‑251 (MQ=255)                                                                                                                                           aCCCCGGTGCGGACATTGTCTTGATTATACCTGTTACTATTTTTCCTGCGTTTAGTATGTCGATGGCTGCATCTGCTGCATCAATAGGTAGAGATGCCACAATCCATACACCGACATTTTCTGAAGCATTGCGTACCAGCCTTATTTTTGGTGCCGTCGACCCCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCCGCCGGTTTGTCCTTAAAGGGATCCACTGGATTGCGTTTGTGctgctg                                                                                           >  2:539493/1‑251 (MQ=255)                                                                                                                                                ggTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTTTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATAtt                                                                                      <  2:491389/251‑1 (MQ=255)                                                                                                                                                ggTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATAtt                                                                                      <  2:472579/251‑1 (MQ=255)                                                                                                                                                                         cTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCGTCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGCATgtttcttg                                                               >  1:509399/1‑242 (MQ=255)                                                                                                                                                                           gCTACTGTTCTTCTGGCGGTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAAGTTTCTGAAGCATTGCGAACCGGCCTTATTTTGGGTGCCGACGAAACCCTGTCGCTGCTGATCGGCTGGGGAATGGGCAGGTTAGCCAGCCTGTGTGTCCTTGAATGGATCCACAGAATGGCGAATGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAg                                                             <  1:539493/249‑1 (MQ=255)                                                                                                                                                                                 gtccttctgGCGTTTGGTATGTCGATTGATGCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTGTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGTGTTGGCCTGGAATGGAACCACTGGATTGCGTTTGTGCTGCGGATATTCCTCGGCGGTCGAATGATTATTGATGGttt                                                        <  2:522810/245‑1 (MQ=255)                                                                                                                                                                                           cGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTCAAGATGCCAACCTCCATAAACAGAAATTTTCTGAAGCCTTGCGAAACGGCTTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGCACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGGATGATTATTGAGGGGTTTTGTGgcgc                                               >  1:355023/1‑247 (MQ=255)                                                                                                                                                                                                             atgCATTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGGCCTTGCATGGAACCCCTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGGGAATGTTTATTGTGGGGTTTCTTGTGGCAGATGTTGTAGATGAAGAGCcg                         >  2:446667/1‑251 (MQ=255)                                                                                                                                                                                                                cATTTGCTGCCTCCATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGACCCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGACAGATTATTGATGGTTTTTGTGGCGGAGGTGCTGAAGGTGCAGAGCcgcgg                      >  2:257648/1‑250 (MQ=255)                                                                                                                                                                                                                 aTTTGCTGCATCAATCGGTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTAGTTTTGGTGCCGGCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCGCTGGATTGCGTTTGTGCTGCTGAGATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGcc                     <  1:258158/251‑1 (MQ=255)                                                                                                                                                                                                                                 ggTAAAGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTg     <  1:478873/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaGATGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGGTTTCGTGGCGCCGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCt  >  1:165150/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GGCACGTACGGACTGAATACTTTCAGTCAGGCGAGACCATATGCACATCAATCGCTATGCCTGCATGAACGCGAAAACCATCACGGCTTGTTGTTCATGTATGCATGTTTGCTGGGGGCGATGATGTGTTTTATGGATACCCCGGTCAGGACATTGTCATGAATATCACTGCTACTGTTCTTCTTGCGTTTGGTATGTCGATGGATGCATTTGCTGCATCAATCGGTAAAGGTGCCACCCTCCATAAACCGAAATTTTCTGAAGCATTGCGAACCGGCCTTATTTTTGGTGCCGTCGAAACCCTGACGCCGCTGATCGGCTGGGGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCT  >  NC_000913/1905530‑1906007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑