Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	499,060	T→G	D305A (GAC→GCC)	aes ←	acetylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	499,060	0	T	G	100.0%	206.4 / NA	65	D305A (GAC→GCC)	aes	acetylesterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/32);  total (33/32)

GGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGG‑TTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGACGGCTGTCATCCAGCAGCGGATCGAACTCCGCCCCGGCAATAAAACAGGGCGGAACTTCGCGAGTGAGATCATTATTAAACAGACAGTAATACGGCGACTCGCGGTCCGCGTCGTTGCTTAAATATGCCTCTTCGTAC  >  NC_000913/498817‑499305                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       ggAGAAAAAGGCGTAGGTCATATTCAGGGGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGAGTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAAGGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTTAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑t                                                                                                                                                                                                                                                <  1:499086/251‑1 (MQ=255)   agaAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑ttt                                                                                                                                                                                                                                              <  1:237578/251‑1 (MQ=255)                      atTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgt                                                                                                                                                                                                                           <  1:481653‑M1/251‑8 (MQ=255)                         cAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATTAAAATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCGAAACTTTTTGTCGCGTATCGCTAAAGCTGAGCGGGAAAGAACTGAGCGCCCTCGCGAAGAGCCTCTGCGGCGGGTTTtctaattctgttgatccgagg                                                                                                                                                                                                                         >  2:451059/1‑232 (MQ=255)                          aGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTTGAAGAGATTGCCGAGCAAAACCGTTAGGTCTTCCTCGGTGCCGGCGATAAAAAATATGAATAGATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTGGTTGCCGCGTAGCACTAAAGCTGAGCGGTGAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTGTCGTCATCCGTTtattcgtaggc                                                                                                                                                                                                                       <  2:337681‑M1/251‑12 (MQ=255)                                 gtgCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagac                                                                                                                                                                                                                <  1:252805‑M1/251‑19 (MQ=255)                                   gCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAAAGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGGAAAGAAATGAGCGCCGTCGCGAAGAGCCCCGGCGGCGG‑GTTTCATCATCCGTGtagtcgtaggccttagacat                                                                                                                                                                                                              >  1:92036‑M1/1‑231 (MQ=255)                                    cccGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatt                                                                                                                                                                                                             <  1:261975‑M1/251‑22 (MQ=255)                                              gctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgat                                                                                                                                                                                                   <  2:483815‑M1/251‑32 (MQ=255)                                                  cGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcga                                                                                                                                                                                               <  1:25044‑M1/251‑36 (MQ=255)                                                   ggATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGGAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑GTTTCATTATCCGGGgagtcggaggcattaaacaattttgcctgatgcgac                                                                                                                                                                                              >  1:406922‑M1/1‑215 (MQ=255)                                                     aTTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCGCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgc                                                                                                                                                                                            <  2:493443‑M1/251‑39 (MQ=255)                                                              agaCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgt                                                                                                                                                                                   <  2:395502‑M1/251‑48 (MQ=255)                                                                     ggAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatc                                                                                                                                                                             <  2:406922‑M1/250‑54 (MQ=255)                                                                                      aaaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcggcttatcaggcctacaaccggtgcc                                                                                                                                                           >  1:138683‑M1/1‑180 (MQ=255)                                                                                       aaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTTCGGCGCTTAATGCCACGCCGGAACCTATCGAAATGATGGCTAATCTTGTTGCCGCGCCTCGCTAAAGCTTAGCGGGAAAGAACTGAGCGCCGTCGCGGAGAGCCTCGGCGGCGG‑GTTTCATCCTCCGTGtagtcggtgggattagacacttttgcctgatgcgacgcttgacgcgcattattaggcctacaaccggtgccg                                                                                                                                                          >  1:400101‑M1/1‑179 (MQ=255)                                                                                         aCCGTGAGGTCCTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAAGGCCACTCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCAGAGCGGTAAAGAACTGATCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcatgcctacaaccggtgccgca                                                                                                                                                        <  1:208717‑M1/251‑75 (MQ=255)                                                                                           cGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTTCGGCGCTTTATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGGGG‑TTTTTATCATCCGTGtaggtgtaggccttagacatttttgcctgatgggaggcttggcgcgtctttttaggcctccaaccggtgcagcctc                                                                                                                                                      >  1:17578‑M1/1‑175 (MQ=255)                                                                                                   cTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATcc                                                                                                                                                                                                                                     <  1:344137/164‑1 (MQ=255)                                                                                                      ccTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattgg                                                                                                                                            <  2:166152‑M1/250‑87 (MQ=255)                                                                                                                     gAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatg                                                                                                                            >  1:294117‑M1/1‑149 (MQ=255)                                                                                                                         aaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgat                                                                                                                        >  1:57706‑M1/1‑145 (MQ=255)                                                                                                                         aaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgat                                                                                                                        >  1:92147‑M1/1‑145 (MQ=255)                                                                                                                         aaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTGGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggggccgcatccggcaattggtgcacaatgcctgatgcgat                                                                                                                        <  1:162594‑M1/251‑107 (MQ=255)                                                                                                                         aaaTATGAATAGATGCCGGCGCTTAAGGACACGCCGAAACATATCGAAATGATGGCGAATCTTGTGGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCTTCGCGAAGAGCCTCGGCGGCGG‑TTGTCATCATCCGTGtagtcgtagtcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctactaccggtgccgcatccggcaattggtgcacaatgcctgatgcgat                                                                                                                        <  2:457905‑M1/251‑107 (MQ=255)                                                                                                                                 atatatTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGCTGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcc                                                                                                                                                                                                       >  1:504022‑M1/1‑137 (MQ=255)                                                                                                                                 atatatTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCTGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatgtgtgcc                                                                                                                                                                                                       <  2:504022‑M1/164‑28 (MQ=255)                                                                                                                                   atatTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgc                                                                                                               >  1:334895‑M1/1‑135 (MQ=255)                                                                                                                                         cGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttat                                                                                                        >  1:196489‑M1/1‑129 (MQ=255)                                                                                                                                         cGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcccgatgcgatgcttgacgcgtcttat                                                                                                        <  1:187217‑M1/251‑123 (MQ=255)                                                                                                                                                  aTGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctac                                                                                               >  1:402261‑M1/1‑120 (MQ=255)                                                                                                                                                    gCCACGCCGGAACATATCGAAATGATGGCGAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACGGAGCGCCGTCGCGAAGAGCCGCGGCGGCGG‑TTTTCAGCAGCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcgtgacgcgtcttatcaggccgacaaccggtgcctcatccgtcaattgttgcacaatgcctgatgcgatgcttgacgcatcgtagcagtcctaca                                                                                              <  2:276071‑M1/250‑133 (MQ=255)                                                                                                                                                     ccACGCCGGCAAATATCGAACTGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGCACTGAGCGCCGTCGCGCAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagccatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgctgcttgacgcatcttatcaggcctacaat                                                                                            >  2:553071‑M1/1‑117 (MQ=255)                                                                                                                                                            ggAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTAGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtacc                                                                                     >  1:398879‑M1/1‑110 (MQ=255)                                                                                                                                                              aaCATATCGAACTGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccgg                                                                                   >  2:484307‑M1/1‑108 (MQ=255)                                                                                                                                                                     cGAAATGATGGCGAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggta                                                                            <  2:402261‑M1/251‑151 (MQ=255)                                                                                                                                                                      gAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAACGAAATGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggt                                                                             >  2:507651‑M1/1‑100 (MQ=255)                                                                                                                                                                       aaaTGATGTCTAATCTTGTTGCCGCGGATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcggcttatcaggcctacaaccggagccgcatccggcaattagtgcaccatgcctgatgcgatgcttgacgcatcttatcaggcctacaatggggaccggatcggtagg                                                                          >  2:447721‑M1/1‑99 (MQ=255)                                                                                                                                                                       aaaTGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtagg                                                                          >  1:425313‑M1/1‑99 (MQ=255)                                                                                                                                                                            atgGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggg                                                                     >  2:180151‑M1/1‑94 (MQ=255)                                                                                                                                                                              ggCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatctgatcaggcctacaatgggtaccggatcggtaggccggat                                                                    <  1:383433‑M1/250‑159 (MQ=255)                                                                                                                                                                                  aaTCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGCGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggc                                                               >  1:268797‑M1/1‑88 (MQ=255)                                                                                                                                                                                  aaTCTTGTTGCCGCGTAGCGCTAAAGCGGAGCGGTAAAGAACTGGGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATAATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaaggggtaccggatcggtaggccggataagg                                                                <  2:14191‑M1/250‑163 (MQ=255)                                                                                                                                                                                      ttgttgCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggaaaaggcgttt                                                           >  2:93296‑M1/1‑84 (MQ=255)                                                                                                                                                                                              gcgTATCGCTAAAGCGGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggatagggcgtttacgccgca                                                   <  2:48448‑M1/251‑176 (MQ=255)                                                                                                                                                                                                    cGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggc                                             <  1:565743‑M1/251‑182 (MQ=255)                                                                                                                                                                                                       aaagtgttggcggtaaagaGATGAGCGCCGGCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGgagtcgtaggcattaggcatgtgggcctgatgcgccgcttggcgcgtctgatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaattggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaag                                          <  2:102932‑M1/242‑185 (MQ=255)                                                                                                                                                                                                            cTGAGCGGTCAAGAACTGAGCGCCGTAGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaataat                                       >  1:461741‑M1/1‑62 (MQ=255)                                                                                                                                                                                                              gAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaataga                                    <  1:71639‑M1/250‑191 (MQ=255)                                                                                                                                                                                                                 cGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑CTTTCATAATCCGTGcagtcgtaggcattagacatttgtgcctgatgcgacgcttgccgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcctcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttccgccgcatccggcaagaatagagcac                                >  2:266470‑M1/1‑57 (MQ=255)                                                                                                                                                                                                                  ggTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgctgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcacc                               >  1:108727‑M1/1‑56 (MQ=255)                                                                                                                                                                                                                  ggTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcacc                               >  1:270918‑M1/1‑56 (MQ=255)                                                                                                                                                                                                                     aaaGAACGGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagt                            <  2:92036‑M1/251‑199 (MQ=255)                                                                                                                                                                                                                      aagaccggagcgccgTCGCGAAGAGCCTCGGCGGCGG‑TGTTCAGCATCCGTTgagtcgtaggctttaggcatgtgtgcctgatgcgacgcttcacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggttcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagtt                           <  2:398879‑M1/244‑200 (MQ=255)                                                                                                                                                                                                                      aaGAACTGCGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcctccggcaagcatagagcacccgtt                           >  2:265082‑M1/1‑52 (MQ=255)                                                                                                                                                                                                                          aCTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtaggcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcagtcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatatagcaccagttaacc                       <  2:400101‑M1/251‑204 (MQ=255)                                                                                                                                                                                                                             gAGCGCCGTCGCGAAGCGCCTCCGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggcaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccga                     >  1:115549‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                             gAGCGCCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaa                    <  1:451059‑M1/251‑207 (MQ=255)                                                                                                                                                                                                                              aGCGCCGTCGCGAAGCGACTCGGAGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgccgcgtcttatcaggactacaaccggtgacgcctccggcaattggtgcacaatgcctgatgcgccgcttgacgcatcttatcaggcataccatgggtaacggatcggtaggccggataaggcgtttacgccgcctccggcaagaatagagaacccgttaacctaac                   >  2:337039‑M1/1‑44 (MQ=255)                                                                                                                                                                                                                               gcgcCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtacc                                                                                     <  1:526387‑M1/184‑142 (MQ=255)                                                                                                                                                                                                                               gcgcCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtacc                                                                                     >  2:526387‑M1/1‑43 (MQ=255)                                                                                                                                                                                                                                 gcCGTCGCGAAGAGCCTCGGCGGCGG‑TTTTCCTAATCCGTGcagtcgtaggtattcgacatttgtgcatgatgcgacgcttgccgcctcttataaggcctacaaccggtgccgcatccggccattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccgggacaggcggttacgccgcatccggcaagactagagcaccagttaaccgcaattc                >  2:511985‑M1/1‑41 (MQ=255)                                                                                                                                                                                                                                          aaGAGCCTCGGCGGCGG‑TTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtatgccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaacttactctgcgcc       <  1:252452‑M1/251‑220 (MQ=255)                                                                                                                                                                                                                                            gagCCTCGGCGGCGG‑TTTTCATCATCCGTAtcgtcgtaggccttagacatttgtgcctgatgccacgcttgacgcgtcttaacaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcacaagttaaccgaacttactctgcgccc      >  2:289441‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                                               ccTCGGCGGCGG‑TTCTCATCATCCGTGtagtcgtaggcattcgacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaacaggcgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaacttactctgcgcccaaat  >  2:240585‑M1/1‑27 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       GGAGAAAAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGG‑TTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGACGGCTGTCATCCAGCAGCGGATCGAACTCCGCCCCGGCAATAAAACAGGGCGGAACTTCGCGAGTGAGATCATTATTAAACAGACAGTAATACGGCGACTCGCGGTCCGCGTCGTTGCTTAAATATGCCTCTTCGTAC  >  NC_000913/498817‑499305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑