Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,393	G→A	intergenic (‑75/+20)	glyQ ← / ← ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,393	0	G	A	100.0%	200.5 / NA	52	intergenic (‑75/+20)	glyQ/ysaB	glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/32);  total (20/32)

CGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTT  >  NC_000913/3725160‑3725636                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    cGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAg                                                                                                                                                                                                                                     <  2:30636/250‑1 (MQ=255)        ggCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAAc                                                                                                                                                                                                                             <  1:74707/251‑1 (MQ=255)            ccAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTAGTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGTATGGAAGATATACAGATCAAAACGGGAAAAAGCAGGGCTTAACGCAt                                                                                                                                                                                                                         <  2:436304/251‑1 (MQ=255)                  cgcgCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGaaa                                                                                                                                                                                                                    <  2:486950/250‑1 (MQ=255)                  cgcgCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAAGGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCGGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGAGCAGTATACCCGCTGGATGGAAGAGATACAGTACAAAACGGGAAAAAGCAGTGCTTAACGCATGGaa                                                                                                                                                                                                                     <  2:519093/249‑1 (MQ=255)                   gcgcAGACAGGGCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGa                                                                                                                                                                                                                  <  2:212745/251‑1 (MQ=255)                    cgcAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGAt                                                                                                                                                                                                                 <  2:410228/251‑1 (MQ=255)                         aCAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGCCCACTGGGTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAGGGTCGGGATCAAGCCCTGGACGGTCCTGGAATCAAACTTTTGCGTATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaa                                                                                                                                                                                                             >  1:521485/1‑250 (MQ=255)                         aCAGGCCATTGGGTGAGAGGTTCCTGCGCCGTCTTCCATGTCCAATGCTTGACGAATGGTGCAGCCCTGGCGAGCCCAGTACTAATGTAAGGTCAGGCTCAAGCACTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGGGGCTTTAAAGGAAGGAATCAGTATACCCGCTGGCTGGAAGATATCCAGTACaaaa                                                                                                                                                                                                                                                  >  2:349481/1‑213 (MQ=255)                               cATTGGGTGAGAGTGTCACGCTCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGAGCAAGCCCTGGAAGGTCCTGGTATCAAACGGTTGCATATGATTTCGTGCTGGATACGTGTGGATTTAAATGAAGGGATCAGTATACCCGCGGGACGGAAGAGATACAGTACAAAACGGGAAAAAGCAGGGCCTAACGCATGGAAAGATGCaaaaaaggg                                                                                                                                                                                                      <  2:53882/251‑4 (MQ=255)                                         gagGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCATGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTTATTGGTGCTGGATACGTGTGGATTTAAAGGGAGGGATCAGTATGGCCGCTGGATTGGAGATATGCATTACAAAACGGGAAAAAGCAGGTCTTAACGCATGGAAAGATGTAAAAAATGGCCATCCGCGt                                                                                                                                                                                            <  1:549614/251‑1 (MQ=255)                                           ggTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCa                                                                                                                                                                                          <  1:451279/251‑1 (MQ=255)                                               cccGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAg                                                                                                                                                                                      <  1:161755/251‑1 (MQ=255)                                                    gcCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAg                                                                                                                                                                                 <  2:144526/251‑1 (MQ=255)                                                      cGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCATGGCGAGCCCAGTACTCCTGTAAGGTCAGGATCAAGCCCTGGAAGGACCTGGTATCAAACTTTTGCATATTATTTCATGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAAAGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGAc                                                                                                                                                                               >  1:543104/1‑251 (MQ=255)                                                         cTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACaa                                                                                                                                                                             >  1:134686/1‑250 (MQ=255)                                                                   cAATGGTTGACCAATGGTGCAGCCCTGGCGAGCCCAGTCATCCTGTAAGGTCAGGATCAAGCCCTGGCAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCttt                                                                                                                                                                   >  2:515572/1‑250 (MQ=255)                                                                              cAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGtata                                                                                                                                                       <  1:463615/251‑1 (MQ=255)                                                                                aTGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAAc                                                                                                                                                     <  1:515572/251‑1 (MQ=255)                                                                                          ccTGGCGAGCCCAGGAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGTTCCTGGTAGCAAACTTTGGCATATTATGTCGTGCGGGAGACGTGTGGAGTTAAAGGAAGGGATCAGTATACCCGCTGGAGTGAATATATACACTACAAAACGGGAAAATGCACGGCTTAACGCAGGGAAAGATGTAAAAAAGGGCCATCCGCGGCAATAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTc                                                                                                                                           <  1:546785/251‑1 (MQ=255)                                                                                                gAGCCCAGTAATGCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGACACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCt                                                                                                                                     >  2:67464/1‑251 (MQ=255)                                                                                                  gCCCAGTAATCCTGTAATGTCAGGATAAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATAAGTATACCCGCTGGATGGAAGATATACAGGAGAAAACGGGAAAAAGCAAGGCGGAACGCATGGAAAGATGCAAAAAAAGGCCATCCGCGGGAAAAGAACAGACAAACCGCGCTTTACGGAACGTATCTCCGCGGATTCCATAGCTTc                                                                                                                                   <  2:283303/251‑1 (MQ=255)                                                                                                  gCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAGACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTc                                                                                                                                   <  1:229358/251‑1 (MQ=255)                                                                                                   cccAGTAATCCTTTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGGATCAAACTTTTGCATATTATGTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTTGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCCTTACGGAAGGTATAACCTCGCATTTCATAGCTTcc                                                                                                                                  <  2:383237/251‑1 (MQ=255)                                                                                                   cccAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCGCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTcc                                                                                                                                  <  2:267241/251‑1 (MQ=255)                                                                                                     cAGTCATCCTTTAAGGTCAGGGTCAAGCCCTGGAAGGTCCTGGTATCAAACTTTGGCATATTATTTCGTGCTGGATGCGTATGGATGTAAAGGAAGTGATCAGTATGCCCGGTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAGCAGACATGCTGCTCCGTACGGAAGGTATAACCGCACGTTTCATAGCTTCCCt                                                                                                                                <  2:341015/251‑1 (MQ=255)                                                                                                      aGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTg                                                                                                                               >  2:157236/1‑251 (MQ=255)                                                                                                       gTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTgg                                                                                                                              <  2:358629/251‑1 (MQ=255)                                                                                                         aaTCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGaa                                                                                                                            <  1:330274/251‑1 (MQ=255)                                                                                                                                    ggAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGAtttt                                                                                                 >  2:96209/1‑251 (MQ=255)                                                                                                                                         gTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCTGTGACGTCGATTTTTTGCt                                                                                            <  1:204601/251‑1 (MQ=255)                                                                                                                                               gTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGaa                                                                                                                                                                                                                                            <  1:412278/101‑1 (MQ=255)                                                                                                                                               gTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGaa                                                                                                                                                                                                                                            >  2:412278/1‑101 (MQ=255)                                                                                                                                                 aTCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGgtg                                                                                    >  2:170455/1‑251 (MQ=255)                                                                                                                                                  tCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGgtgt                                                                                   >  1:250933/1‑251 (MQ=255)                                                                                                                                                                  tattTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGCAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTGGCCGGTGTTTTAAGGTTTTgccgc                                                                   >  2:271296/1‑251 (MQ=255)                                                                                                                                                                       cGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAATCGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCTCATTTCATAGCTGCCCTGGAACTGTTCGAAGGCGGTGACTTCGATTGTTTGCTAGCCGGTGTTAGATCGTTTTGCCGCCTGac                                                              <  2:81572/251‑2 (MQ=255)                                                                                                                                                                               aTACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGTTGGATGTAAGATATCCAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAACATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTAAGGAAGGTATAACCGCGCATTTAATAGCTTCCCTGGAAATGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTgaat                                                      >  1:535531/1‑248 (MQ=255)                                                                                                                                                                                 aCGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATa                                                     >  2:57879/1‑250 (MQ=255)                                                                                                                                                                                            tAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACACAACGGGAAAAAGCAGGGCTTAACGCATGGACAGATGCAAAAAATGGCCATACGCGTCAAAAGCACAGACACACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcct                                         >  1:245172‑M1/1‑243 (MQ=255)                                                                                                                                                                                               aggaaggGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTGCCCTGGAACTGTTCGAAGGCGGGGACGTCGATTTTTTGCGCGCCGGGGTTATAACGTTTTTCCGCCTGATCCTTGAATAACGCatcggcctcgg                                      <  2:245172‑M1/251‑12 (MQ=255)                                                                                                                                                                                                  aaggGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggatt                                   <  1:408072‑M1/251‑15 (MQ=255)                                                                                                                                                                                                   agggatacgtataccagATGGATGGAAGATATACAGTACAAACCGGGAAAAAGCAGGGCTTAACGCATGGACAGATGCAAAAAATGACCATCCGCGTCAAAAGAACAGAAAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCATCCCTGGAACTGTTCGAAGGCGGTGACGTCGCTTTTTTGCTCGCCGGTGTTCTAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattc                                  >  2:397242‑M1/9‑236 (MQ=255)                                                                                                                                                                                                        tCAGTATACCCGCTGGATGGAAGATATACAGTACAAACCGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCAATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtt                             >  1:53079‑M1/1‑231 (MQ=255)                                                                                                                                                                                                         cAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacggt                             <  2:155824‑M1/250‑21 (MQ=255)                                                                                                                                                                                                                cccGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGGATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGGCGATTTTTGGCTGGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtg                     <  1:397242‑M1/251‑29 (MQ=255)                                                                                                                                                                                                                  cGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgtt                   >  1:230059‑M1/1‑221 (MQ=255)                                                                                                                                                                                                                    cTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACCCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGAATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgttttgtactgtat                  >  1:99113‑M1/1‑219 (MQ=255)                                                                                                                                                                                                                      ggatggaAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgttt               <  2:134686‑M1/251‑35 (MQ=255)                                                                                                                                                                                                                         tggaAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGACTGGAAAAATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttg            >  1:39598‑M1/1‑214 (MQ=255)                                                                                                                                                                                                                                   aCAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATTGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagc  <  1:271296‑M1/251‑48 (MQ=255)                                                                                                                                                                                                                                     aGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAg                                                                                                                  <  1:165643/137‑1 (MQ=255)                                                                                                                                                                                                                                     aGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAg                                                                                                                  >  2:165643/1‑137 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    CGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTT  >  NC_000913/3725160‑3725636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑