Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,768	C→T	W362* (TGG→TGA)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,768	0	C	T	100.0%	248.4 / NA	73	W362* (TGG→TGA)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/32);  total (41/32)

GCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCGGAGTCACAGC‑ACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATAC  >  NC_000913/3468522‑3468993                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       gCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAgg                                                                                                                                                                                                                                    >  1:172258/1‑251 (MQ=255)      aTGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTc                                                                                                                                                                                                                               <  1:123368/251‑1 (MQ=255)                 gcGACAGAAGTTTTATAATGTGCTTCTTTATATGCTACGATGCTGTCCGCGCTGTTCGCTTTGCTGGCCGGGAAACCATCCGGAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACAta                                                                                                                                                                                                                    <  2:324281/250‑2 (MQ=255)                          gTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGaa                                                                                                                                                                                                           <  2:451845/251‑1 (MQ=255)                          gTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCATGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGa                                                                                                                                                                                                            >  2:512302/1‑250 (MQ=255)                              tataATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACTCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAAt                                                                                                                                                                                                        <  2:84223/250‑1 (MQ=255)                               ataATGTGCATAGTTATAATCTACGATGATGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCAGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGTAGAGGTAGATGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTTATCGTTGGTATCCCGTGGGCAATCTTTAGACCCAACCCTTCAGGCGTTCTTCTAAACATTGCCATTGACAATa                                                                                                                                                                                                       >  2:67053/1‑250 (MQ=255)                                 aaTGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAAc                                                                                                                                                                                                     >  2:235184/1‑250 (MQ=255)                                  aTGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACACATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACAt                                                                                                                                                                                                   >  1:537068/1‑251 (MQ=255)                                    gttcATCGTGATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTGATTGGAGTTTTCAGCGCTGGTACGCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATgg                                                                                                                                                                                                 <  2:548817/248‑1 (MQ=255)                                    gtgCATCGATATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAATCATCCGCAACAATGACGCCGCCGTTATCCCTTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGCCGCCATGGGTTATTGGAGTTTTCAGAGCCGGTCCCCCGTGGGCAATCTTCAGACCCAACCCATCAGGCGTTCTTGTAACCATTACCATTAACAATAACTTgg                                                                                                                                                                                                 >  2:268601/1‑251 (MQ=255)                                          cGTTATAATCTCCGATGCTTTCCGCGCTGTACGCTTTGCTGCCCTGGAAACCGTCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAtt                                                                                                                                                                                           <  2:446060/251‑1 (MQ=255)                                             tataATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCg                                                                                                                                                                                        >  2:92022/1‑251 (MQ=255)                                                aaTCTACGATGCGGTCCGCGCTGGACGCTTTGCGGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGTCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACAGGGTAAAGTTCGCTa                                                                                                                                                                                     <  2:215475/251‑1 (MQ=255)                                                    tACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCt‑t                                                                                                                                                                                >  2:165485/1‑251 (MQ=255)                                                    tACGATGCTGTCCGCGCTGTACGCTTTGATGGCCTGGAAAACCTCCGCAACAATGACGACGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAAAATTACCATTGAAAATAACATGGTAAATTTCGCTACCt‑t                                                                                                                                                                                >  2:540360/1‑251 (MQ=255)                                                       gATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCt                                                                                                                                                                             >  1:96318/1‑251 (MQ=255)                                                        aTGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTg                                                                                                                                                                            >  1:507332/1‑251 (MQ=255)                                                          gCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGc                                                                                                                                                                          >  1:149390/1‑251 (MQ=255)                                                          gCTGTCCGCGCTGTACCCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGc                                                                                                                                                                          <  2:363220/251‑1 (MQ=255)                                                          gCTGGCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTGCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTgg                                                                                                                                                                           <  1:257286/250‑1 (MQ=255)                                                             ggccGCGCTGTCCGCTTTGCTGGCCGGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAGTTCGCTACCT‑TCCTGGcc                                                                                                                                                                         <  2:89827/247‑1 (MQ=255)                                                               ccGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTc                                                                                                                                                                     <  1:278891/251‑1 (MQ=255)                                                                cgcgCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGGTCTTGTAAACATTCCCATTGAAAATAACATGGTAACTTTCGCTACCT‑TCCTGGCCGCTcc                                                                                                                                                                    >  2:398267/1‑251 (MQ=255)                                                                   gcTGTCCGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGCCGCCATGGGTTATTGGAGTTTTCCGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACg                                                                                                                                                                 >  2:274174/1‑251 (MQ=255)                                                                   gcTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCCTGGGTTATTGGCGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACCTTACCCTTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTccccg                                                                                                                                                                 >  2:338599/1‑248 (MQ=255)                                                                    cTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGTCGCCGCCGTTATCCATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGAAGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATACCATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑c                                                                                                                                                               >  1:239041/1‑251 (MQ=255)                                                                             ttGCTGGCCTGGAACCCATCCGCAACAGTGACCCCGCCGTTATCAATTTCACAGGGAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGCACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGTAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGTTCCACG‑CTTGCAAt                                                                                                                                                        <  2:467735/249‑1 (MQ=255)                                                                             ttGCGGGCCTGGAAACCCTCCGCAACAAGGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAAt                                                                                                                                                        <  2:154833/249‑1 (MQ=255)                                                                                   gCCTGGAACCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATc                                                                                                                                                <  2:96318/251‑1 (MQ=255)                                                                                   gCCTGGAACCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATc                                                                                                                                                <  1:100606/251‑1 (MQ=255)                                                                                       gtaaaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGtt                                                                                                                                             <  2:292130/248‑1 (MQ=255)                                                                                        gAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCGTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTTCCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTaa                                                                                                                                           <  1:274174/251‑1 (MQ=255)                                                                                         aatccacccGCAACAATGACGCCTACGTTACCAATTTCACAGGTAGTCGGGTTACCGTATTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGCCCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAAc                                                                                                                                          <  1:147710/244‑1 (MQ=255)                                                                                          aaCCATCCGCAACAATGGCGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTGCCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACg                                                                                                                                         <  1:243297/251‑1 (MQ=255)                                                                                             cATCCGCAACAATGACGCCGCCGTTATCCATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCtt                                                                                                                                      >  1:221025/1‑250 (MQ=255)                                                                                             cATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCt                                                                                                                                       >  1:7630/1‑250 (MQ=255)                                                                                                 cccaacaaGGCCGCCGCCTTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGTTACCCCGTGGGCAATCTTTAGACCCACCCCATCAGGCTTTCTT‑TTAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGGAATCGTTAACGCTATCCGc                                                                                                                                 <  1:439986/249‑1 (MQ=255)                                                                                                   caacaaTGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTCCCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGACAATAACATGGTAACTTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGCGTAATCGTTAACGCTATCGGCt                                                                                                                                >  2:47688/1‑251 (MQ=255)                                                                                                     tcccggttcccgccgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCt                                                                                                                                <  2:135812/240‑1 (MQ=255)                                                                                                          ggcgccgcCGTTATCAATTTCACAGGTAGGCGGTTTACCGTACTGACTCAAGTCCGCTGCGGTAGCTGTTCGCTCGAGACGCCATGGGGTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAAGCTTTAGAGCCAGCCCATCAGGCGGTCTTGTAAACAAAACCATTGAAAAGATCATGGCAGATTTCGCTACCG‑TCCTGGCGGCTGCACG‑CTTGCTAGGAGCAATCGGTGACGCGTTCCGCTGGTGTTg                                                                                                                         <  2:235121/249‑1 (MQ=255)                                                                                                              ccgccgTTCTCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCt                                                                                                                                >  1:135812/1‑240 (MQ=255)                                                                                                                gccgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCt                                                                                                                   >  2:295490/1‑251 (MQ=255)                                                                                                                gccgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCt                                                                                                                   <  1:59084/251‑1 (MQ=255)                                                                                                                 ccgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTACCGCTATCCGCTGGTGTTGATGGCTc                                                                                                                  >  2:442177/1‑251 (MQ=255)                                                                                                                  cgTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACAGTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCGATCCGCTGGGGTTGATGGCTCg                                                                                                                 <  1:461921/251‑1 (MQ=255)                                                                                                                        cAATTTCACAGGTAGGCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCACGCTGtt                                                                                                            <  2:221025/250‑1 (MQ=255)                                                                                                                          aTTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGCTCGCTGTACGCTCGAAACGCCATGGGTTATTGGAGTTTTCAGCGCAGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTACACATTACAATTGAAAATAACATGGTAACTTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATc                                                                                                         >  1:557958/1‑251 (MQ=255)                                                                                                                            ttCACCGGTAGTCGGCCTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCg                                                                                                       >  1:431336/1‑251 (MQ=255)                                                                                                                            ttCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGGTGGCTCGCTGTTATCCg                                                                                                       >  2:437561/1‑251 (MQ=255)                                                                                                                                                 gTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGttt                                                                                  >  2:23737/1‑251 (MQ=255)                                                                                                                                                 gTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATCGCTTATTGGAGTTTTCAGCGCAGACACCCCGCGGGCAATCTTTAGACAAAACCCATCAGGCGTACCTGTAAAAATTACCATTGAAAATAACATGGCAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGCAATCGTTAACGCTATCCGCTGGCGTTGATGGCTCGCTGTTATCCGGCGTTGGGGTCACCGGTGttt                                                                                  >  1:364343/1‑251 (MQ=255)                                                                                                                                                   aCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGttgcc                                                                                <  2:187427/251‑4 (MQ=255)                                                                                                                                                   aCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATATTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑cc                                                                               >  1:146062/1‑251 (MQ=255)                                                                                                                                                     tGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCgg                                                                             >  2:19771/1‑251 (MQ=255)                                                                                                                                                      gACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCg                                                                              <  2:149390/249‑1 (MQ=255)                                                                                                                                                                cGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCaa                                                                 <  1:235184/251‑1 (MQ=255)                                                                                                                                                                 gCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGCGTTTTCAGCGCTAGTACCCCGTGCGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCCTTGAAAATAACATGGAAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAAc                                                                >  1:532770/1‑251 (MQ=255)                                                                                                                                                                   aGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGCTGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCg                                                              >  2:202408/1‑251 (MQ=255)                                                                                                                                                                   aGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGAAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCg                                                              >  2:207324/1‑251 (MQ=255)                                                                                                                                                                       gTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCGGAGt                                                          >  1:462319/1‑251 (MQ=255)                                                                                                                                                                         cgCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCATCGCTGGTCCCCCTTTGGCAATCTTTAGACCCAACCCATCAGGCGTTCGTGTAAACATTACCAATGAAAATAACATGGTAAATTTCGCTACCA‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGCGTTGGGGTCACCGGTGGTT‑CCGG‑AATTGGATCAACCGGAGTc                                                         <  2:537068/249‑1 (MQ=255)                                                                                                                                                                          gCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATAAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCGGAGTcac                                                       >  1:239348/1‑251 (MQ=255)                                                                                                                                                                           cTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCGGAGTcac                                                       >  1:19006/1‑250 (MQ=255)                                                                                                                                                                                  cTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCACTCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGATGGGGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGCGTTT‑CCGG‑AGTTGGATCAACCGGCGTCACAGC‑ACCGc                                              >  2:328367/1‑251 (MQ=255)                                                                                                                                                                                     gagaCGACATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGCAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCGGAGTCACAGC‑ACCGCCat                                           >  2:187359/1‑251 (MQ=255)                                                                                                                                                                                          gCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCGGAGTCACAGC‑ACCGCCattatt                                       >  2:449426/1‑250 (MQ=255)                                                                                                                                                                                            cATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCGGAGTCACAGC‑ACCGCCATTATTATc                                    >  1:65852/1‑251 (MQ=255)                                                                                                                                                                                                    aTTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGACAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGCGTTGGGGTCACCGGTGTTT‑CCGG‑CGTTGGATCAACCGGAGTCACAGC‑ACCGCCATTATTATCCGGCTTa                             >  1:554187/1‑250 (MQ=255)                                                                                                                                                                                                         aGTTTTCAGCGCAGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCGGAGTCACAGC‑ACCGCCATTATTATCCGGCTTAACGGa                        >  1:435372/1‑250 (MQ=255)                                                                                                                                                                                                               cAGCGCGGGTCCCCCGGGGGCAATTTTGAGACCCACCCCATCAGGCGTTTTTGTAAACATTACCATTAAAAAAAACATGTAAAATTTCGCT‑CCTGTCCGGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCTCTGTTGTTGATGGCTCGCTGTTATCCGGATTTGGGGTCACCG‑TGTTTTCCGG‑AGTTGGATCACCCGGAGTCACAGC‑ACCGCCATTATTATCCGGCTTAACGGAACAGGa                  <  1:227369/250‑1 (MQ=255)                                                                                                                                                                                                                         ccccggTGGGCAATCTTTAGACCCACCCCGTGAGGCGTTCTTGAAAACATATCAATTGAAAAAAAAATGGTAAATTTCCCT‑CCC‑TCCCGGGCGCCCCCCGGCTTCCAAGGAGTAATCGTAAACGATACCCGCGGGTTTTGGTGGGTCGCTGTTATCGGGGGTGGGGGGCACCGGT‑TTT‑CCGGGAGTTGGATCAACCGGAGTTACTGC‑ACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAc       <  1:512302/246‑1 (MQ=255)                                                                                                                                                                                                                              gtgggaattCTTTAGACCCACCCCATAAGGCGTTTTTGAAAACATTACCATGAAAAAAAACAGGGTAAATTCCGCTCCCT‑TCCTGGCCGCTCCACG‑TTTCCAATGAGTAATCGTAAACGCTATCCCGTGGTTTTGTTGGGGCGCTTTTACCCGGAGGTGGGGTCCCCGGTTTTT‑CCGG‑ATTTGGTGCACCCGGTGTCTC‑GCTACCGCCGTTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATAc  <  1:93073/243‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       GCCCCATGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCT‑TCCTGGCCGCTCCACG‑CTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTT‑CCGG‑AGTTGGATCAACCGGAGTCACAGC‑ACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATAC  >  NC_000913/3468522‑3468993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑