Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,9840TG32.1% 78.6 / 12.0 57L41V (TTG→GTG) cycAD‑serine/alanine/glycine/:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (5/33);  new base G (17/1);  total (23/34)
Fisher's exact test for biased strand distribution p-value = 4.25e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AATCTTAGTATTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCAC  >  NC_000913/4429737‑4430231
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                               
aaTCTTAGTATTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGgtt‑gt                                                                                                                                                                                                                                                             <  2:395063/251‑1 (MQ=255)
         aTTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTTG‑TACGGGGTT‑GTTTAT‑GGGGt                                                                                                                                                                                                                                                   <  2:296848/251‑1 (MQ=255)
         aTTCAACCCGCGTATTGTTACCTAATATCGATGAGTCCCTATACAGATTCTTCGTATCATAGACAGACTAAAGGCCGTATAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGTTAGATCAGGTAAAAGTCGTTGCCGATGATCACGCTCCGGCTGAACAGTCTCTACGGCGCAAGCTCACAAACCGACATATTCAGCTTATTTCCATTGTCGGT‑GCC‑AATAG‑TACCTGGTA‑GTATAT‑GGGGt                                                                                                                                                                                                                                                   <  2:336278/251‑1 (MQ=255)
            cATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGTGTT‑GTTTAT‑GGGGTCt                                                                                                                                                                                                                                                 <  2:311943/250‑1 (MQ=255)
             aTCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑CTTTG‑GACGGGGGT‑GTTTTT‑GGGGTGTgg                                                                                                                                                                                                                                               >  2:163916/1‑251 (MQ=255)
              tcacgcgTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑CTTGG‑TTCGGGGTT‑GTTTTT‑GGGGTGTGG‑c                                                                                                                                                                                                                                             >  2:111509/4‑251 (MQ=255)
                 cgcgTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACAGATTCAGCTGATTGCCAGTGGCGGG‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑Caaa                                                                                                                                                                                                                                          <  1:42144/251‑1 (MQ=255)
                     tATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGGGTACGGG‑TT‑GTTTTT‑GGGGTGTGG‑CAAAACg                                                                                                                                                                                                                                       >  1:313016/1‑250 (MQ=255)
                      aTTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCCCATTG‑‑AACGGGGGT‑GTTTTTTGGGGTCTG‑‑CAAAACggt                                                                                                                                                                                                                                     >  1:415262/1‑249 (MQ=255)
                                   atCGATGAGTCACGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTCG‑TCCGGGGGT‑GGTTTT‑GGGGGCTGG‑GCAAACGAGTAGCCTTGACggg                                                                                                                                                                                                                        >  1:482153/1‑251 (MQ=255)
                                        ggAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTc                                                                                                                                                                                                                   <  2:112885/250‑1 (MQ=255)
                                              ccGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCAt                                                                                                                                                                                                            <  1:111509/251‑1 (MQ=255)
                                                      gATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGttt                                                                                                                                                                                                    <  1:7115/251‑1 (MQ=255)
                                                             cgtATCATAGACTGCCTAAAGGCCGTAGCGCCTGAACAACACAGACCGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGC‑GCC‑CATGG‑GACGGGGGT‑GTTTTT‑TGGGTCTGG‑CAAAACGAATTGCCTTGCCGGGCCGTCG‑AGCAttttttttttttttat                                                                                                                                                                                             >  2:216170/1‑239 (MQ=255)
                                                             cgtATCATAGACTGACGAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGa                                                                                                                                                                                             <  2:244735/250‑1 (MQ=255)
                                                                              aaaGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTCATGct                                                                                                                                                                             <  1:434953/249‑1 (MQ=255)
                                                                                            cTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑GACGGGGGT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTTGTTTAT‑ATGATCATTGGTTTTTTTCTCTTTTTTGTGATTCgg                                                                                                                                                             >  1:116815/1‑251 (MQ=255)
                                                                                              gAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGGCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCgg                                                                                                                                                             <  2:512043/249‑1 (MQ=255)
                                                                                                   acacAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑TGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTGCGTTTAT‑TTGATCATTGGTTTTATGCTCTGTTTCGTGATGCGGGC‑AAT‑gg                                                                                                                                                    <  2:366260/251‑1 (MQ=255)
                                                                                                            ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑GACGGGGGT‑GTTTTT‑GGGGGCTGG‑CCAAACGATTAGCCt                                                                                                                                                                                                                               >  1:94871/1‑171 (MQ=255)
                                                                                                            ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATGGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCt                                                                                                                                                                                                                               <  2:94871/171‑1 (MQ=255)
                                                                                                                       aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTTGCA‑ATTG‑‑TACGGGGTTTGTTT‑T‑GGGGTGTGGGCAACA‑GATTTGGCCTGCCCGGGCGTCG‑GTCCTTTTCGTTTATCATG‑TCATTGGTTTTTTGCTCTTTTTCGTGATGCGGGG‑CAC‑GGGGGGATTGCTTCTTTCTAAt                                                                                                                                >  2:15571/1‑251 (MQ=255)
                                                                                                                          aaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACTGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GGTTAT‑GGGGTCTGG‑CAAAACGATTAGCGTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑AGGATCATTGGTTTTATGCTCTTGTTCGTTATGCGGGC‑AGT‑GGGGGGATTGCTGCTTTCGAATCTg                                                                                                                             <  1:217246/251‑1 (MQ=255)
                                                                                                                          aaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCTTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATc                                                                                                                               <  1:398298/249‑1 (MQ=255)
                                                                                                                               aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑CTTGG‑GACGGGGGT‑GTTTTT‑GGGGGTTGG‑CAAAAAGATTAGCCTTTCCGGGGCGTGG‑GTTATTTTCGTTTTT‑ATATTCATTGGTTTTATTCTCTTTTTTGTGATGCGGGC‑AAT‑GGGGGGATTGTTTCTTTCTAAttttgatt                                                                                                                         >  2:369336/1‑243 (MQ=255)
                                                                                                                                     gATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑CTTTG‑TTCGGGGGT‑GTTTAT‑GGGGGCTGG‑CAAAACGATTTGCCTTGCCCCGCCGGCG‑GCCACTTTCGTTTAT‑ATTGTCCTTTGTTTTTTTCTCTTTTTCATGTAGCGGGC‑AATTGGGGGAAT‑GCTCCTTTTGGATTTGGAATACCAATc                                                                                                                  >  1:317182/1‑251 (MQ=255)
                                                                                                                                     gATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAAGCTGGAATACAAATc                                                                                                                  <  1:163916/251‑1 (MQ=255)
                                                                                                                                      aTCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACAGAAATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTAG‑GACGGGGAT‑GATTTT‑TGGGTCTGG‑CAACAAGATTTGCCTTGGCGGGGCGTCGCTTCTTTTTC‑TTTTT‑ATTATTATTGGTTTTATGCTTTTTTTATTGATGCGGGC‑ACT‑GGGGGAGTTTGTGGTTTTGCATCTGGAATACaaaact                                                                                                                 >  1:339487/1‑248 (MQ=255)
                                                                                                                                        cAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCtcc                                                                                                               <  1:286222/251‑3 (MQ=255)
                                                                                                                                          ggTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TCCGGGGGT‑GTTTAT‑GGGGTCTGG‑GAAAACGAATAGGCTTTCCGGGGCGTCG‑ATCATTTTTGTTTTT‑ATTATCATTGGTTTTATGc                                                                                                                                                                              >  1:136918/1‑188 (MQ=255)
                                                                                                                                          ggTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGc                                                                                                                                                                              <  2:136918/188‑1 (MQ=255)
                                                                                                                                             aaaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑CTTGG‑GACGGGGGT‑GTTTTT‑GGGGGCTGG‑CCAAACGATTAGCCTTTCCGGGGCGTCG‑GTCATTTTTGTTTAT‑TTGGTCCTTTGTTTTTTGCTTTTTTTCGTGATGGGGGCGAAT‑GGGGGA‑TTGCTTCTTTCTCATTTTGAATACAAATTTTTTtgtg                                                                                                          >  2:167346/1‑251 (MQ=255)
                                                                                                                                                          gatgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑TGC‑ATTGG‑CAAGGGGGT‑GTTTATTGGGGT‑TGG‑CAAAAAGAATAGGCTTGACGGGGCGTCG‑GTCCTTTTCGGTTAT‑ATGATCCTTGGTTTTATGCTCTTTTTTGTGATGCGGGC‑AAT‑GGGGGGATTGCTGCTTTCGAATCTGGAATACAAATATTTTAATGAATTCGCTTCCgg                                                                                             >  1:249447/1‑250 (MQ=255)
                                                                                                                                                          gatgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTTG‑TACGGGGTT‑GTTTTT‑GGGGGCTTG‑CAAAACGATTAGACTTTCCGGGCCGTCG‑ATCATTTTTGTTTTT‑ATGATCATTTGTTTTTTTCTCTTTTTCGTGATTCGGGG‑AAT‑GGGGGGATTGCTGCTTTCGGATCTGGAATACAAATCTTTTAGTGACTTCGCTTccc                                                                                              >  1:94009/1‑249 (MQ=255)
                                                                                                                                                                  ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑CTTGG‑TACGGGGGT‑GTTTTT‑GGGGTCTTG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGGTGCGGGC‑AAT‑GGGGGGATTTGTGCTTTTGAATCTGGAATAACAATCTTTTTTTGACTTCGCTTCCGATTTACTCg                                                                                     >  1:316659/1‑251 (MQ=255)
                                                                                                                                                                            gAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTgg                                                                             <  1:330454/249‑1 (MQ=255)
                                                                                                                                                                                 gTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGc                                                                                                                                                                              <  1:481501/149‑1 (MQ=255)
                                                                                                                                                                                 gTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGGT‑GTTTTT‑TGGGGCTGG‑CAAAACGATTAGCCTTTCCGGGCCGGCG‑ATCATTTTTGTTTAT‑ATGATCATTGGTTTTTTTCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTTCTGCTTTTGAATCTGGAATACAAAACTTTTTGTGTCTTCGCTTCCGATTTTTTCGGGCCGTGGGCAgggt                                                                      >  1:219531/1‑249 (MQ=255)
                                                                                                                                                                                   cGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGTTTTTATGCTCTTTTGCGTTATGCGGGC‑AAT‑TGTGGAATTGCTGCGTGCGAATCTGGAATAGAAAGCTTGTAGTGACTTGGCTTCCGGGTTATTCGGTCCTGGGGCAGG‑atat                                                                   <  2:552368/251‑1 (MQ=255)
                                                                                                                                                                                       aCGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTc                                                                <  1:58902/250‑1 (MQ=255)
                                                                                                                                                                                       aCGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGA‑GGC‑ATTGG‑TACGGGGGT‑GTTTTT‑GGGGTGTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑CTCCTTTTCGTTTAT‑TTGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTTCTGCTTTCGTATCTGGAATACAAATCTTTTTGTGACTTTGCTTCCGGTTTATTCGGGCCGGGGGCAGG‑ATATTTcc                                                               >  1:411053/1‑250 (MQ=255)
                                                                                                                                                                                         ggCGCAATCTCACAAACCGACATATTCAGCTTATTGCCCTTGGCGGG‑GGC‑CTTGG‑GACGGGGGT‑GGTTAT‑AAGGACAGG‑CCAAAAGCTTAACCTTGCCCGGGCGCCG‑ACCCCATTCGTTTAT‑ATTGTGATTGGTTTTATGCTCTTTTTCGTGCTACGGGC‑AAT‑GGGGGGATTGTCGCTTTCGCATCTGGAATACAAATCTTTTAGGGTCTTCGCTTCCGCTTTTCTCGGGCCGGGGGGAGG‑GTATTTTAcc                                                             >  1:357625/1‑251 (MQ=255)
                                                                                                                                                                                         ggCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTTG‑TACGGGGGT‑GTTTAT‑TGGGGCTGG‑GAAAACGATTAGCCTTGGCGGGCCGTCG‑ATCATTTTTGTTTTT‑ATGGTCATTGGTTTTATGTTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGGATTGCTGCTTTTGAAACTGGGATACAAATATTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATAttttatc                                                             >  1:416773/1‑247 (MQ=255)
                                                                                                                                                                                         ggCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCAcc                                                             <  2:411053/251‑1 (MQ=255)
                                                                                                                                                                                               aTCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTTG‑TTCGGGGGT‑TTTTTT‑TTGGTCTGG‑CAAAACGATTAGCCTTTCCCGGCCGGTG‑CTCATTTTTGTTTTT‑TTGATCATTGGTTTTTTGCTCTTTTTTGTTATGCGGGG‑CAT‑GGGGGGGTTTCTGGTTTTGGATCTGGGATACAAAACTTTTTGTGGCTTTGCTTCCGATTTACTCGGGCCGTGGGCAGGGAT‑TTTTACCGGCTgg                                                       >  2:191513/1‑251 (MQ=255)
                                                                                                                                                                                                   cacaAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGAGTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTGTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAAGCTGGAATACAAATCTTTTAGTGACTTCGCTGCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTgg                                                       <  2:282217/247‑1 (MQ=255)
                                                                                                                                                                                                    acaAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑GACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAAGCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTa                                                  <  2:179711/251‑1 (MQ=255)
                                                                                                                                                                                                      aacccGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTGCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACt                                                <  2:415262/248‑1 (MQ=255)
                                                                                                                                                                                                           gACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACTGGTTc                                           <  1:369336/251‑1 (MQ=255)
                                                                                                                                                                                                               aatTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTG‑GTTTAT‑GGGGTCTCG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACTGGTTctgct                                       <  2:160011/250‑1 (MQ=255)
                                                                                                                                                                                                                atTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGGCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTGTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCGTTGAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCTGCTGGAATTACGGGTTctgct                                       <  2:387590/250‑1 (MQ=255)
                                                                                                                                                                                                                    aGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGtt                                  <  2:416773/251‑1 (MQ=255)
                                                                                                                                                                                                                     gcgtATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGGTGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTg                                 <  1:373701/248‑1 (MQ=255)
                                                                                                                                                                                                                         aTTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCGTGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAg                                                                         <  1:351440/208‑1 (MQ=255)
                                                                                                                                                                                                                                    cGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAg                  <  1:460349/251‑1 (MQ=255)
                                                                                                                                                                                                                                        ggCC‑AGTGG‑TACGGGGGT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGGGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTAACCGGCTGTACTTACTGGTTCTGCTGGGTGGTAGCCGGTAATGCAGACg               <  1:198993/250‑1 (MQ=255)
                                                                                                                                                                                                                                                gg‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGtggtgg         <  2:220648/250‑1 (MQ=255)
                                                                                                                                                                                                                                                       gggTT‑GTTTAT‑GGGGTCTGA‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCAc  >  1:219293/1‑251 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                               
AATCTTAGTATTCATCCCGCGTATTGTTACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGACAGGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGT‑GCC‑ATTGG‑TACGGGGTT‑GTTTAT‑GGGGTCTGG‑CAAAACGATTAGCCTTGCCGGGCCGTCG‑ATCATTTTCGTTTAT‑ATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGC‑AAT‑GGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGG‑ATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCAC  >  NC_000913/4429737‑4430231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑