Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,027,2330TA31.7% 51.9 / 14.6 41intergenic (+5/+376)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base T (22/6);  new base A (0/13);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.54e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGG  >  NC_000913/4026983‑4027474
                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 
gAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCCGGGTCCCGGGACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTT‑GTGATGGGGGAAAATGCCCGCGATCTGTTTTG‑TCGTGAGCGGAATGCGCGGGGAGATA‑CCCTGTTTTG‑GGA‑TGC‑GTT‑TGCATTTTTTTGAGatat                                                                                                                                                                                                                                                   >  1:438940/1‑251 (MQ=255)
gAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGatat                                                                                                                                                                                                                                                   <  2:447912/251‑1 (MQ=255)
        ttCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGATTTCAGGGGCACGGGACGCTGGCCGAGCATGATATTTATATTTCCGGGCGTTTTTGGAAGGGGGAAAATTGCC‑GCGGGCTGTTTTT‑CAGTGAGGGGAAAGCGCGGGGAGATC‑GCCTGTTTGG‑GGA‑GTC‑GTT‑TTCATTTTTTTGAGATATAAAAAAAc                                                                                                                                                                                                                                           >  1:408385/1‑251 (MQ=255)
          cGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAAcc                                                                                                                                                                                                                                          <  1:269068/250‑1 (MQ=255)
           gTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTACCGGCGGCATTTGACGATCAACGCACGCTGGAAGAACATGGTATTTATCTTTCCGGGCGTTTT‑TAGCTGGCGAAAATTGCCCGCCATCTGTTTTG‑CAGTGGGCGGAATGCGCGGGGAGATT‑GCCTGTTTTG‑AGG‑AGC‑GTT‑TGCATTTATCTGTGATATaaaaaaaaccc                                                                                                                                                                                                                                        >  1:269350/1‑247 (MQ=255)
                                aGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGGAGGATCACGGGACGCTGGCAGGGCATGATATTTATTTTGCCGGGCGGTTT‑GAGGTGGCGGAAAATGCCCGCGATCTGTTTTG‑CAGTGAGCGGAATGCGCGGGAAGATC‑GCCTGTTTGG‑GGG‑TGC‑GTT‑TGCCTTTATCTGAGATATAAAAAAAACCCCCCCCGACAGGCGGggagaa                                                                                                                                                                                                                    >  1:333509/1‑250 (MQ=255)
                                                    aacCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCCGGGACACGGTACGCTGGCAGAGCATGATATCTTTATTTTCGGACGTTTTTGGGTGGGGGAAAATTACCC‑CGATCTGTTTTTTCGGT‑AGCGGAATTCGCGGGGAGATC‑GCCTGTTTGG‑GGA‑TGC‑GTT‑TGCATTTTTTTTAGGTATTAAAAAACCCGCCCCTGACAAGCGGggagaaaacgcaatcaaccgttttttt                                                                                                                                                                                               >  2:432226/1‑230 (MQ=255)
                                                                gCTGGCGGGGGCGTTCGGGCACCGTGTTAGCGGCGGTATTGCAGGATCACGGTCCGCTGGCAGAGCAGGATATCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTTTTTTG‑CATGGAGCGTAATGCGCGGGAAGATC‑GCGTGTTGGG‑CGA‑TGC‑GTT‑GGCATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGa                                                                                                                                                                                   <  1:177905/251‑1 (MQ=255)
                                                                       ggggCGTACGGGCACCGTGTTACCGGCGTTATGGAAGGATCACGGTACGCTGGCAGAGCATGATATCTATATGGCCGGACTTTTT‑GAGATGGCAAAAATTGCCCGCGTTCTGTTTTG‑CAGTGAGCTTATTGCGCGGAAAGATC‑CCTTGTTTGG‑CGT‑TGC‑GTT‑TGATTTTATCTAAAATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGa                                                                                                                                                                            <  1:300678/250‑1 (MQ=255)
                                                                        gggggTACGGGCACGGTTTTAACGGCGGTATTGCAGGATCCCGGTACGCGGGCAGAGCATGTTATTTATATTGCCGGTCGTTTT‑GAGATGGCAAAAATTGCCCGGGTTCTTTTTTG‑CAGGGGGCGTATGGCGCGGGAAGATC‑GCTTGTTGGG‑CGT‑TGC‑GTT‑TGCATTTATCTGAAATAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGAc                                                                                                                                                                           <  2:25575/247‑1 (MQ=255)
                                                                           ccgttctgCACGGTGTTAACGGGGGTATTGCGGGATCAGGGTACGCGGGCAGAGCATAATTTCTATTTTGCCGGACGTTTA‑GAGATGGCGAAAATTGCCGGCGATCTGTTTTG‑CAGTGAGCGTAAGGGGGGGGAAGATC‑GCGTGTTTGG‑GGA‑TGC‑GTT‑TGCATTTATCGGAGATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGt                                                                                                                                                                         <  1:112342/243‑1 (MQ=255)
                                                                                          ttacgggcggTATGGCAGGATCGGGGTAGGCGGGCAGAGCATAATATCATTATTGCCGGCCGTTTG‑GGGATGGCAAAATTTGCCCGCGATTTGTTTTG‑CAGGGAGCGAAATGCGCGGAAAGATC‑GC‑TGTTTGGGCGT‑TG‑‑GTTGAGCGTTTATCGTAAATATAAAAAACCCCGCCCCTGACAGTCGGGAAGAACGGCAACTAAACTGTGATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATg                                                                                                                                                          <  2:229322/245‑1 (MQ=255)
                                                                                           aaaCGGCGTTAGTGCAGGATCCGGGTCCGTTGGCAGAGCATGATATCATTATTGCCGGAGGTTTG‑GAGAGGGCAAAAATTGCCCGCAGTCTGTTTTG‑CAGGGAGCTTAAGGCGCGGAAAGCTC‑GCTTGTTTGG‑CGA‑GGC‑TTT‑GGAATTTATCTGAAAAAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGcc                                                                                                                                                        <  2:11193/250‑1 (MQ=255)
                                                                                            aactgcggTATGGCAGGATCACGGTACGCTGGAAGAACATGATATCTATTTTGCCGGAGGTTTT‑GAGGTGGCAAAATTTGCCCGGGGTCTGTTTGG‑CAGTGAGCGGAATGCGGGGTAAAATC‑GCCT‑TTTGG‑GGAAGGC‑GTT‑GGAATTTATCTGAGAAAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGccc                                                                                                                                                       <  2:316664/247‑1 (MQ=255)
                                                                                               ggcggTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTT‑GCGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGACGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCCTTTAGCTTTATAACGTATCTGGCAAACCATGCCCGa                                                                                                                                                     >  1:538141/1‑250 (MQ=255)
                                                                                                   gTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCg                                                                                                                                                 >  1:441141/1‑250 (MQ=255)
                                                                                                     atggCAGGATCACGGTACGCGGGCAGAGCATGATATCTATATGGCCGGACGTTTT‑GAGATGGCAAAAATGGCCCGCGTTTTGTTTTG‑AAGTGGGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑GGA‑TGC‑GTT‑TGCATTTATCTGAAAAAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGAc                                                                                                                                               <  2:441141/247‑1 (MQ=255)
                                                                                                               cACGTTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTTTTTTG‑CGGTGAGCGTAATGCGCGGAAAGATC‑GCCTTTTTGG‑CGA‑TGC‑TTT‑TGCATTTATCTGAAATAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAAGCATGCCCGATGCGACGCTGTCGCGTc                                                                                                                                    <  1:494668/251‑1 (MQ=255)
                                                                                                                  ggTACGCTGGCAGAGCCTGATATCTATATTGCCGGACGTCTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTCATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAACAACCCGACCCTGACCGGCGGGAAGAAACGCAACTAAACTGTTATTCAGTGGAATTTACATCTATGACGTAACTGGCAAAACATACCCGATGCGACGCTGTTGCGtgttt                                                                                                                                 >  2:279011/1‑247 (MQ=255)
                                                                                                                      cGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTT‑GAGATGGCGACAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCCGTGGCATTTTGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGt                                                                                                                             >  1:488479/1‑251 (MQ=255)
                                                                                                                          ggCAGAGCATGATATCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTTTCGTGCCt                                                                                                                         >  1:458152/1‑251 (MQ=255)
                                                                                                                             agagCATGATATCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTAc                                                                                                                       >  1:294722/1‑250 (MQ=255)
                                                                                                                                  atgttatatatagtccgggtctttgt‑GAGAGGGAGAAAATTGCCCGCGATCTGTTTGG‑CAGTGAGCATAATGCG‑GGGGAGATCCGCTTGTTGTG‑CGA‑GGCTGTT‑T‑CATTTATCTGATATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGTCGTAGCTGACAAACCATGCCTGATGCGACGCTGTCGTGTCTTAGCGTGCCTACAAATAg                                                                                                                 <  2:516561/226‑1 (MQ=255)
                                                                                                                                     atatCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTTGCAAACCATGCCCGATGGTACGATGTCGCGTCTTATCGTGCCTACAAATAGtac                                                                                                              >  1:126145/1‑249 (MQ=255)
                                                                                                                                          tataTTGCGGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAAGGCGGGGAAAGATC‑GCTTGTTTGG‑CGA‑TGG‑TTT‑TGCATTTATCTGAAATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAAc                                                                                                          <  1:324864/250‑1 (MQ=255)
                                                                                                                                           tatagttccggactttgggagaGGGCAAAAATTGCCCGCGATCGGTTTTG‑CGGTGAGCGTAAGGCGCGGGAAG‑TC‑CCCCGTTTGGGCGT‑TGA‑TTT‑TGCTTTTACCTGAGATATAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGGATCTGGCAGACCATGCCCGTTGCTACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAAc                                                                                                          <  1:85756/232‑1 (MQ=255)
                                                                                                                                            tatTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGatat                                                                                                                                                                                                                                                   <  2:475350/111‑1 (MQ=255)
                                                                                                                                            tatTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGatat                                                                                                                                                                                                                                                   >  1:475350/1‑111 (MQ=255)
                                                                                                                                                 ccGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGGAGAACGGCAAATAAACTGTTATTAAGTGGCATTTAGATCTATGGCGTATCTGGCAAACCATGCCCGGTGCGACGCCGTCGCGTCTTATCCTGCCTACCAATAGTCCGAACCGCAGGcc                                                                                                  >  1:257109/1‑251 (MQ=255)
                                                                                                                                                        tttt‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAAAGGCCACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGGATCTGGCAAACCATACCCGATGCGACCCTGTGGCCTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATa                                                                                             >  1:220779/1‑249 (MQ=255)
                                                                                                                                                             gagaTGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACTGCAAATAAACTGTTATTCAGTGGCATTTAGATCTATGGCGTATCTGGCAAACCCTGCCCGATGCGACGCCTTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAggagt                                                                                       >  2:55774/1‑248 (MQ=255)
                                                                                                                                                               gaTGGCGAAATTTGCCCGCATTCTTTTTGG‑CAGTGAGCTTAATGCGCGGAAATATC‑GCTTGTTTGG‑CGA‑TGC‑TTT‑TGCATTTATCTAAAATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGttt                                                                                     <  1:224352/251‑1 (MQ=37)
                                                                                                                                                                    agaaattggcccgggttCTGTTTTG‑CGGGGAGCGTATGGGGGGGGAAGACC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGAAAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTAcgcc                                                                                <  2:284944/235‑1 (MQ=37)
                                                                                                                                                                                        ttttG‑CAGTGAGCGTAATGGGCGGAAAGATC‑GCTTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGAAAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCa                                                             <  1:432226/250‑1 (MQ=255)
                                                                                                                                                                                                         aTGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGGTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACg                                            >  1:7245/1‑251 (MQ=255)
                                                                                                                                                                                                                ggAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAAAGGCCACTAAAATGTTATTCAGAGGCATTTAGATCTATGAAGTATCTGGCAAACCAAGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAAACATCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGGGCCTGATGCCTGATGCGCCGCTGTcgc                                     >  2:275532/1‑251 (MQ=25)
                                                                                                                                                                                                                 gAAGACC‑GCTTGTTTGG‑CGT‑TGC‑GTT‑TGCATTTATTTGAAATATAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTcgcg                                    <  1:170488/251‑1 (MQ=255)
                                                                                                                                                                                                                     aTC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCCACTAAAATGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACCCTGTCGCGTCTTATCGCGCCTAAAAAAAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCCCATCCGGCAATTGGGGCATGATGCCTGATGCCCCGCTGGCGCGTCtt                                >  2:31096/1‑251 (MQ=37)
                                                                                                                                                                                                                       c‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCACCCATGACAGGCGGGAAAAACCGCAACAAAACAATTATTTAATGGCATTTTGATCTACGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAAACGTAGGCCGGAAAAGGAGTTTACGCCGCATCCGGCATTTGGTGCATGATACCTGATGCGACGCTGTCGCGCCTTAt                              >  1:530049/1‑251 (MQ=21)
                                                                                                                                                                                                                          gccttttgggggA‑TGC‑TTT‑TGCTTTTATCGGAAATAAAAAAAACCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATc                             <  2:430166/240‑1 (MQ=21)
                                                                                                                                                                                                                              ttggg‑CGA‑TGC‑GTT‑TGCATTTATCTGAAATAAAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGc                         <  1:170331/247‑1 (MQ=255)
                                                                                                                                                                                                                                      a‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAAATAAACTGTTATTCCGTGGCATTTAGGTCTATGACGGATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGCCCGAACCGTAGGCCGGATAAGGCGGTTACGCCGCCTCCGGCCATTGGTGCATGAAGGCTGATGCCACGCTGTCGCAGCTTATTGTGTCTAccaa                  >  2:109502/1‑247 (MQ=25)
                                                                                                                                                                                                                                              t‑tGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCtt                                                                                                                                  >  2:488151/1‑132 (MQ=255)
                                                                                                                                                                                                                                                    tttATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGGCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCTTGCCTACAAATAGTCCGAACCg      >  2:54246/1‑251 (MQ=255)
                                                                                                                                                                                                                                                        tCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGCACGGCAACTAAACTTTTATTCCGTGGCTTTTTGATCTTTGACGTATCTGGTAAACCATGCCCTTTGCGACACTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTACGCCGGCTAAGGCGTTTACGCCGCCTCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTTTCGTGCCCACAAATAGTCCGAACCGTAgg  >  1:392378/1‑251 (MQ=21)
                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 
GAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTT‑GAGATGGCGAAAATTGCCCGCGATCTGTTTTG‑CAGTGAGCGTAATGCGCGGGAAGATC‑GCCTGTTTGG‑CGA‑TGC‑GTT‑TGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGG  >  NC_000913/4026983‑4027474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑