| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,878,947 | 0 | C | T | 26.1% | 88.0 / 13.9 | 47 | E276K (GAA→AAA) | cas1 | multifunctional nuclease Cas1 |
| Reads supporting (aligned to +/- strand): ref base C (5/29); new base T (12/0); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCGCCAGTCCAGCTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATAACCAGCTGCAAGTATCGCCG > NC_000913/2878699‑2879195 | accGCCAGTCCAGCTATTTGTTCCCAGATCATTTCACGAATTTTTGCTGATACAGCTCCTACATATACCCCTGCACGTACCTCCAACAGCCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATGGCAGCTTCTCCGATGGCCGGCATCTCCCAGTGAAACAGGAAGAGGAATGGCAACGGGCGGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTtct < 2:107415/249‑1 (MQ=255) gTCCAGCTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGACGCGGAATGGCAACAGGTTGTGCATCTTCAGGTGGGGCCGGCGGCTGTTTTtctaccacggc > 2:248316/1‑243 (MQ=255) gCTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTTTGTTTCTcccccggccagcc > 2:251579/1‑240 (MQ=255) cTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCCACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGCACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTTTTTTTCCAGCGG‑CCAGCAc > 1:334344/1‑251 (MQ=255) ttCCCACAGCATTTCACGAAGTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAACCCTCCTCTTAAGCGCGGAGGTGCATTTTCATTGACCACGACCAACACACTCATCTCAGCTACTCCGATGGCCTGCATCGCCCAGTGAAACATGAAGCTGAATGGCAGCAGGCTTTGCACCTTCAGTTTGGGCCGGCGGTTGTATGTCGCCTGCGT‑CAAGCATGTCctc < 2:526330/250‑1 (MQ=255) cccAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTAGTTCTCCAGCGG‑CAAGCACGTCCTCtat < 1:422848/251‑1 (MQ=255) tCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATc < 2:131375/250‑1 (MQ=255) ccaaTTTTTGCGGATTCATCCCCTACCAATACCCCCGCACCTTCCTCCAACAACCAAAATGCTTATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑tt < 1:432225/249‑1 (MQ=255) ttttGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCTGAATCAA‑TTTGGc < 1:457693/250‑1 (MQ=255) ttttGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGGATTTTTCCAGCGG‑GAAGCACGCCCTCTATAAGCGGAAACAA‑ATTGGCt > 1:51051/1‑251 (MQ=255) tttGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGGATTTTTCCACCGG‑GAAAAACGTCCACCATTAGCGGGATCAAGTTTggt > 2:149135/1‑249 (MQ=255) ggATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGtt < 1:261393/251‑1 (MQ=255) aCATCACCTACATACACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTGCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGGGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGGCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTta < 2:96235/250‑1 (MQ=255) cTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTTTTCCGCGG‑CAAGCACGTCCTCTCTAAACGGAATCAA‑TTTGGGTAATGTTTTACTACTGCg > 1:149211/1‑251 (MQ=255) cATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTGTCCAGCGG‑CAAGCACGGTCGCTATAAACGGGATCAA‑TTTGGGTAATGtttttctcgtgctca > 1:315983/1‑239 (MQ=255) aCCCCTGCAAGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGAGGGGCCGGCGGTTGGATTTTACCCGCGGGCAA‑CACACCCTCCATAAGCGGAAACAA‑TTTGGCTAATGGTTTACAACTGCG‑acaaacat > 2:418780/1‑248 (MQ=255) cTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCCGCGG‑CAAGCACGTCCCCTATAAGCGGAATCa > 1:208103/1‑214 (MQ=255) cTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCa < 2:208103/214‑1 (MQ=255) aCCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGcc < 1:299282/250‑1 (MQ=255) aacaacCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGGACATTGTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGa < 2:130398/251‑1 (MQ=255) acCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTc < 1:426832/251‑1 (MQ=255) gCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCAc < 1:312594/249‑1 (MQ=255) gTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGGATCAA‑TTTTGCTAATGTTTTTCTACTGCG‑AAAAATATACCTGCACGCCAAACGGACTTCCCCGTCCGGcccccccgggtt > 1:452713/1‑240 (MQ=255) aaGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTTTTCACCGG‑CCAGCACGTACTCTTTAAACGGGAGCAA‑TTTGGGTAATGTTTTTTTACTGTGCAAAAAT‑TCCCTGTACGCCCAACGGGTTTACCCGTCCGGCGCAccccgggtac > 2:307856/1‑243 (MQ=255) aaGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTa < 2:149211/250‑1 (MQ=255) aGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGGGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGCGTTacg < 2:524046/251‑1 (MQ=255) aGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTTTCCACCGG‑CGCGCACGTCCCCTATAAGCGGAATTAA‑ATTTGCTTATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCCAACAGACTTCTCGGTCCGGCTCACCCGGGGTACGACGCGCCATCTCcaaaact > 1:327681/1‑248 (MQ=255) aGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGGCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCt < 2:364673/251‑1 (MQ=255) gACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTc < 2:95542/251‑1 (MQ=255) aCCACGACCAACATACTCATTTCAGCTACTCCGATGGCATGCCTCTCCCAGAGAAACAGGAAGCGGAATGGCAACAGGATGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTTTCCAGCGG‑GAAGCCCGTCCTCTATAAGAGGAATCAA‑TTTGGCTACTGTTTTTCTACTGCG‑CAAAATATACCTGCACGCCAAAAGGACTTCCCCGTCCGGGTCCCCCCGGTTACGACGCGCTATCTCTAAAgacttcc > 1:63070/1‑245 (MQ=255) gACCAACATACTCATTTCAGCTACTCCTATGGACTGCATCACCCAGGGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCGCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAAGATCCCTGCACGCCAAACGGCCTTCCCGGTCCGGCGCACCAGGGTGACGACGCGCTATCACAAAAGCTTTCGGtgca < 2:45549/251‑4 (MQ=255) aaCTCATGTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTGTTACTACTGCG‑AAAAATATCCCTGCGCGCCAATCGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCGCTCGGTACAACAGTGTc < 2:334344/250‑1 (MQ=255) cAGCAACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTGCCCTGTCCGGCTCACCAGGGTTACGACGCGCTATCGCAAAAGCTTTCGGTACAACAGTTTCAAATTTAat < 2:219308/251‑1 (MQ=255) acgccGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAatgat < 2:124053/246‑1 (MQ=255) ccGATGGCCTGCATCTCCCAGTGAAACATGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAAGATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTGTCGGTACAACAGTGTCAAATTTAATGATGTCTg < 2:535071/251‑1 (MQ=255) aGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAatgatg < 2:111410/228‑1 (MQ=255) aGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATATTCAGGTGGGGCCGGCGGTTGTATTTTTCCACGGG‑CCAACACGGCCTCTATAAACGGAATCAA‑TTTGGGTAATGGTTTTCTACTGCG‑AAAAATATCACTGCACGCCCAAAGGGCTTCCCGGTCCGGCTCACCAGGGGTACGACGCGCTATCTCAAAAACTTTCGGTACAACAGTGTCAAATTTTatgatgagatcgggagagcacacgccta > 1:111410/1‑228 (MQ=255) aaaCAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTTTCCAGCGG‑CAAACACGTCCTCTTTAAGCGGAATCAA‑TTTTGCTAATGTTTTTCTACTGCG‑AAAAATATCCCCTCACGCCCAACGGAATTCCCCGTCCGGGTCACCCAGGTTTCGACGCGCTATCTCAAAAGCTTTCGGTACAAAAGGGTCAAATTTAATGATGTCTGCAATATCGTAAAAAAAAGaaaa > 1:38795/1‑249 (MQ=255) gCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCt < 2:403205/251‑1 (MQ=255) ggAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGATGTATTTTTTCCGCGG‑CAAGCACGTCCTCTATAAGCGGAAACAA‑TTTTGATAATGTTTTACTACTGCG‑AAAAAAATCCCTGCACGCCAAACGGACTTCCCGGTCCGGGCCACCCGGGGTACGACGCGCTATCTCAAAAACTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTgg > 1:238987/1‑250 (MQ=255) aTGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATg < 1:8429/251‑1 (MQ=255) aaCAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTTTCCACCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATTCCTTCACGCCAAACGGACTTCCCGGTCCGGCTCACCCGGGTTTCGACGCGCTATCTCAAAAAATTTCGGTACCACAGGGTCAAAATTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCCTTCCTGTATTcaca > 1:171350/1‑250 (MQ=255) aGGCTGTGCATCTTCAGGTGGTGCCGGCTGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAAGATTCCTGCACGCCAAACGGTCGTCTCGTTCCTGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTGCGGTACAACAGTGTCAAATTTAATTATGTCGGCGATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAAcc < 1:100639/251‑1 (MQ=255) cAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGc < 1:149135/250‑1 (MQ=255) cggcggTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATAACCAGCTg < 1:191540/251‑1 (MQ=255) ggcggTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATAACCAGCTGc > 1:112791/1‑251 (MQ=255) ggcggTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGATAAGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAACTGGTGCATAACCAGCTGc > 1:399859/1‑251 (MQ=255) gTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGGAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATAACCAGCTGCAAGt < 2:171350/251‑1 (MQ=255) gTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTAGCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCGGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATAACCAGCTGCAAGTATc < 1:248316/251‑1 (MQ=255) tctcCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTGCCTTTATGCACAAACCCAATAGCTGGTGCATAACCAGCTGCAAGTATcgccg < 1:541598/250‑1 (MQ=255) | TCCGCCAGTCCAGCTATTTGTTCCCAGATCATTTCACGAATTTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGG‑CAAGCACGTCCTCTATAAGCGGAATCAA‑TTTGGCTAATGTTTTACTACTGCG‑AAAAATATCCCTGCACGCCAAACGGACTTCCCGGTCCGGCTCACCAGGGTTACGACGCGCTATCTCAAAAGCTTTCGGTACAACAGTGTCAAATTTAATGATGTCTGCAATATCGTAAACAAAGGAAAGAGGCTTTCCTGTATGCACAAACCCAATAGCTGGTGCATAACCAGCTGCAAGTATCGCCG > NC_000913/2878699‑2879195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |