| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,828,506 | 0 | T | G | 31.2% | 59.4 / 18.7 | 65 | V257G (GTG→GGG) | srlD | sorbitol‑6‑phosphate 2‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (11/33); new base G (18/2); total (30/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGTTGGCGTGGGCCGTTC > NC_000913/2828256‑2828754 | gAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGt < 1:142529/251‑1 (MQ=255) ggCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGTTGA‑TGtt < 2:190195/251‑1 (MQ=255) gCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTc < 1:4574/251‑1 (MQ=255) aTTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACGGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCTACAAAGTACCGCTCAAACGCGTCTGCGAGTATCAAGATGTGCTGAATATGCGGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTTCTG‑GACCGGACTGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTg < 1:262935/251‑1 (MQ=255) aTTACGGTGCATTAACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGATGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACCGTCGATCAATGTCACCGGCGGGCAGGTGA‑TGtttgg > 2:263509/1‑248 (MQ=255) tACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGGTCTggt > 1:265684/1‑249 (MQ=255) ccggggCATTCACTGATGCTCGGTAACCGGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGAt < 2:531127/245‑1 (MQ=255) ggTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGAGTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGTACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCaa < 1:364815/251‑1 (MQ=255) tCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATAAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCACGATGTGCTGAATATGCTGCTGTTCTA‑CGCCACTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGCAAGGTGA‑TGGTCTGATCAACAGCGGGGATCCATTa > 1:267127/1‑250 (MQ=255) ggTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGA‑TGTTCTGATCAACAGCGGAGATCCATTaa > 1:102843/1‑249 (MQ=255) aaCCTGCTGAAATCTCCTATTTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAAACGGATCCAGTGGAGTAGTATT‑ACATCGACAAAGTACCGCTCAAACCCGGCTGCGATTATCAAGATGGGCTGAATATGCTGCTGTTCTA‑CGCCAGGCCGAAGGCGTCGTACTG‑CACCGGACAGTCGAGCAATGTGACCGGTGTTCATGTGA‑TTTTCTGATCAACAGGGGATATGCATTAAGGagc < 2:270756/251‑3 (MQ=255) gctgAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGGACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTcc < 2:561070/249‑1 (MQ=255) ccGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGGATT‑ACATCGACCAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAg < 2:27287/251‑1 (MQ=255) gTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATAGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTTAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCTTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGAGCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATg < 1:281928/250‑1 (MQ=255) cAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCTGGGGG‑TGTTCTGATCAACAGCGGAGATCCCTTAAGGATCTCCGTGAGACTATAGAATGCCTgg > 1:395066/1‑250 (MQ=255) cAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGCGA‑TGTTCTGATCAACAGCGGGGATCCATTAAGGATCTCCGTTAGACTATAGAATGCCTg > 1:15946/1‑250 (MQ=255) aGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGGCCGGGGG‑TGTTCTGCTCAACAGCGGGGATCCATTAACGATCTCCGTGGGACTATAGAAGGcccgtt > 2:525037/1‑247 (MQ=255) gTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCt < 1:3238/249‑1 (MQ=255) cgACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGGGACTATAGAATGCCTGATGCGCTCAGCTCCTCAGGCAt > 1:329177/1‑250 (MQ=255) gCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGTGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACt > 1:190011/1‑251 (MQ=255) gggTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTc < 1:328285/250‑1 (MQ=255) ggTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCg < 1:308071/251‑1 (MQ=255) ccaacccGGATCAAGTCGAACA‑TATTTACATCTAAAAAGTCCCGACCAAACGCGGCTGCGATTATCAAGATGTGCTAAATATGCTGCTTTTCTC‑CGCCGGTCCTAAGGCGTCTTACTC‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TTTTCTAATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGCCTATAGAATGCCTGATGCGCTACTCTCATCAGGCATACAGGGTTTCCGCCAc < 2:313948/246‑1 (MQ=255) cGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGG‑TGTTCTGATCAACAGCGGGGATCCATTAAGGATCTCCGTGAGACTATAGAAAGCCTGCTGCGCTACGCTCATCAgggctaaagggctttcgcccccacatt > 2:261530/1‑226 (MQ=255) ggATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTTTGGTCAACAGCGGGGGTCCCTTTAGGATCTCCGTGGGGATTTAGAATGCCCGGTGCGCTCCGCCCATCCGGCCTCCACGGCTTTCGCCACCACAttt > 2:560853/1‑250 (MQ=255) aTCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGGGATCCATTAAGGGTCTCCTTGAGACTATAGAATTCCCTATGCGCTACGCTCATCAGGCATAAAAGACCTCCGCCACTACATTa > 2:438538/1‑249 (MQ=255) aaGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGAATTCCGCCACTACATTAAGGa > 1:328100/1‑250 (MQ=255) ggtcatgattaactCGACAATGTCCCGCCCAAACGCGGCCGCTATTGTCAAGTTGTACAAAATTTGCTGCT‑TTCTAACTCCAGTCCTAAGTCATCTTCCGG‑CTCCGGAGATTCGATCAGGCTCACCGGCGGCCATGTAA‑TGTTCTGTTCAACAGCGGAAATCCTTTAAGATTCTCCGGGTGACTATAAATTGCCGGTTGCCCTACGCTCTTGAGGCATAAAGGACTTCCGCCACTACATTAAGGAAAAGtt < 1:303136/238‑1 (MQ=255) gTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGc < 1:381289/211‑1 (MQ=255) aCATCGACAATGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCGGAATATGCTTCTGTTCTA‑CCCCAGTCCTAAGGCGTCGTCCGG‑CACCGAACAGTAAATCAATGTCACCGGTGGTCAGGGGA‑TGTTCTGATCAACAGGGAAGACCCATTAAGGATCGCCGGAAGACTATAGAATGCCTGAGGCGCTACGCTCAGCAGTCATACGGGTCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCg < 1:81759/251‑1 (MQ=255) aCATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGG‑TGGTCTGATCAACCGCGGAGGGCCATTAAGGATCTCCGGGAGACTATAGAATGCCTGATGCGCTACGCTCATTAGGCCTACACGACTTCCGCCACTACCTTAAGGAAAGGTTATGGTATCCg > 2:131481/1‑251 (MQ=255) ccGACAAAGTACCGCTAAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCTTACTG‑CACCGGACAGTCGAACAATGGCGCCGGCGTTCAGTTGA‑TGTTCTAACCAACAGCGGAGATCAATTAAGGATCTCATGGAGCCTATAGAATGCCTGATGCTCTACGCTCATCAGGCTTACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCAc < 1:362283/250‑1 (MQ=255) cGACAAAGTACCGCTCAACCGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGCCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCGGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGTCATACAGGTCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACt < 1:525037/251‑1 (MQ=255) gACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAAGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCCACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtc > 1:12305/1‑251 (MQ=255) aCAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGGCACCGGCGGTCAGGTGG‑TGTTCTGATCAACAGCTGAGGTCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGc < 1:535192/200‑1 (MQ=255) cAAAGTCCCGCTCAACCGCGGCGGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCATCCCTAAGGCGTCGTACTG‑CCCCGGACAGGCGATCAATGTCCCCGGCGGTCAGGTGA‑GTTTCTGATCAACAGCGGAGATCCATTAAGTCTCCCGGTGAGACTATAGAATGCCTGATGCGCTACCCTCATCAGCCAGACAGTACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtcat < 1:263509/251‑1 (MQ=255) cAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCCTTAAGGATCTCCCGGAGACTATAGAATTCCTGATGGGCTACGCCCATCCGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGGATcccccatcat > 1:418919/1‑243 (MQ=255) aaaaGTCCCGCTCAAACGCGGCTGCGATTACCAAGATGTGCTAAATAGGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CCCCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATAAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACtcat < 1:9974/250‑1 (MQ=255) aaaGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAACAGCGGGGATCCATTAAGGATTTCCGTGAGACTATAGAAAGCCTGGTGCGCTACGCTCATCAGGCATACAGGGCTTCCGCCACTACATTAAGGAAAAGTTATGGGATCCGCACtcctc > 2:363649/1‑248 (MQ=255) ccGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCGTTTCTC‑CGCCAGCCCAAAGGCTTCGTACTG‑CCCCGGACAGTCGATCAATGTCCCCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGTTCCATAAAGGATCTCCGTGAGACTATAGAATGCCTTATGCGCTACGCTCATCAGTCATACAGGGCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTc < 1:80472/251‑1 (MQ=255) cGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGG‑TGTTTTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGGCTATAGAATTCCTGATGCGCTACGCTCCTCCGGCATACAGGACTTCCGCCACTACATTAAGGAAAAATTATGGTATCCGCACTCATCACCGTCg > 1:150679/1‑251 (MQ=255) aaaCGCGGCGGGGATTTACAGGATGTGCTGAATAGTCTGATGTCCTC‑CGCGAGTCCTAAGGCGTCGT‑CTGGCACCGGACAGTATATAAATGTCACCGGCGGTCAGGTGA‑TGTTCTGAACAACAGGGGAGACCCATTAAGGATCTCCGTGAGACTATAAAATGCCTGATGCGCTCCGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGtt < 2:434108/251‑1 (MQ=255) cgcgGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGAATGT‑ATGGTCAACAGCGGAGATCCATTAAGGCTCTCCGTGCGACTCTAGAATGCCCGATGCGCCACGCCCATCAGGCATACAGGACCTCCGCCCCCACATTAAGGGAAAGGTATGGTATCCGCACTCCTTCCCGTCGcccctatc > 1:326212/1‑245 (MQ=255) gggttgtgtttataaagttttgCTGAATATGCTGCTGTTCTA‑CGCAGGTCCAAAGGCGTCGTACTG‑CCCCGGACAGCCGTACAATGTCCCCGGCGGTCAGGTGA‑TGTTCGAATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAAGGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGc < 1:17492/231‑1 (MQ=255) gCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTTTAGAATGGCTGATGCGCTACGCACATCCGGCATAAAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCTCCCTTATCCCCTggg > 1:329091/1‑250 (MQ=255) aTCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGG‑TGTTCTGATCAAAAGCGGGGCTCCATTAAGGATCTCCGTGAGACTATATAATGCCCGATCGGCTACGCTCATCAGGCCTAGAGGACTTCCGCCACTCCATTAAGGAAAAGTTATTGTATCTACACTCATCCCCGTCGCCGTTAATGCCTGGGGTgcgc > 1:570925/1‑251 (MQ=255) cAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGTTCAAAAGCGGAGATCCCTTAAGGCTCTCCGTGGGACAATAGAATGCCTGGTGCGCTACGCCCATCAGGCCCACCGGACTTCCGCCCCTACATTAAGGAAAAGTTATGGTATCCCCACTCATCACCGTCCCCGCTATCGCCTggggggggcc > 2:93954/1‑242 (MQ=255) aGATGTGCTAAATATGCTGCTGTTGTC‑CGCCAGTCCAAAGGCGCCGTACGG‑CCCCGGAAAGTGGATCAATGTCACCGGCGGTCGGGTGA‑GGTTCGGATCAACAGCGGAGATCCATTAGGGATCTCCGGGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACt < 2:155398/251‑1 (MQ=255) aGATGTGCGAAATATGTTGCTGTTCTA‑CGCCAGCCGTAAGGCGTCGTACTG‑CACGGGACAGTCGATCAATGTCACCGGGGGTCAGGTGA‑TTTTCTGATCAACAGGGGAGATCCATTAAGGATCTCGGTAATACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACTGTACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACt < 1:410278/251‑1 (MQ=255) gctaaaTATGCTGCTGTTCTC‑CGCCAGGCCAAAGGGGTCGTCCTC‑CCCCGGCCAGTCGATCAATGTCCCCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAAGGCCTGAGGCGCTACGCCCAGCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑tt < 1:29592/247‑1 (MQ=255) atGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCCGGGGA‑TGTTATGGTCCACAGCGGGGATCCATTAAGGGTCTCCGGGAGAATATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCCCTACATTAAGGGAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGGGGGCGCAACTGGCC‑TTAggcggc > 1:506515/1‑251 (MQ=255) ggtgtgcgaggcatgTCCTAAGGCGTCGTACTG‑CACAGAAGAGACGAGAAATGTCACCGGCGTTCAGGTGT‑TTATATAAGAAACAGAGGAGGTCAAATAAGGATCGCGGCGAGATTATACATAGCCTGAGGGGCATCGCGCATCATGCATAAAGTACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACGCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGc < 1:69489/236‑1 (MQ=255) ccaggccaaaGGCGTCGTCCTG‑CCCCGGACAGTCGATCAATGTCCCCGGGGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATAAAGGATCTCCGGGAGACTATAGAAGGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGtt < 1:560853/243‑1 (MQ=255) aGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTCTGATCAACAGCCGAGATCCATTAAGGGTCTCCGTGAGACTATAGAATGCCTGATGCGCCTCGCTCATCAGGCATACAGGACTTTCGCCACTACATTAAAGAAAAGATATGGGATCCGCACTCATCACCGTCGCCCTTATCGCCCGGGGTGCGCCACTGGCC‑TTAGGCGGCTGGCAAATTTTTCGtttt > 2:154695/1‑251 (MQ=255) aGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGGGA‑TGTTATGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATATAATACCCTATGCGCTACGCTCATCAGGCATACAGGACATCCGCCACTACATTCAGGAAAAGTTATGGTATCCGCACTCATCACCCCCCCCGTTATTGCCTTGTTTGCGCAAACGGCC‑TTCAGCGGCTGGCACATTTTTCGtttt > 2:172970/1‑251 (MQ=255) cgtcgtACTG‑CACCGGTCAGTCGATCAATGTCACCGGCGGTCCGGGGA‑TGTTCTGATCAACAGCGGAGATCCACTAAGGGTCTCCGTGGGACTATAGAAAGCCTGATGCGCTTCCCTCCTCAGGCATACAGGGCTTTCGCCACTACATTAAGGAAAAGTTATGGTATCCGCCCTCATCCCCGTCCCCGTTATCGCCTGGTGTGCGCCACTGGCC‑TTAGGCGGCTGGCCAATTTCTTGTTTTactcgggcct > 2:432542/1‑242 (MQ=255) cgtACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCCGGTGA‑TGGTCTGGTCAAAAGCGGAGATCCCTTAAGGGTCTCCGTGGGACTATTGAATGCCTGATGGGGTACGCTCATTAGGCATACAGGACTTCCGCCACTACATTAAGGGAAAGTTATGGTATCCGCACTCATCACCGTCGCCGGTAACGCCCGGTGTGGGCCACTGGCCCTTAG‑CGGCTGGCCAATTTTTCGTTTTTACCATGCCtttc > 2:171688/1‑249 (MQ=255) ccGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCACCAGCGGAGATCCATTAAGGATCTCCGTGAGACTAGAAAATGCCGGATGCGCTACGCTAATCAGGAATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTAt < 1:438538/251‑1 (MQ=255) gtcAGTCGATCAAGGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATAAAGGATCTCCGTGAGACTATAGAATGCCTGAGGCGCTACCCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGcc < 1:432542/249‑1 (MQ=255) caaagccaatcatgacccccGGCGGTCAGGTGA‑TGTTCTAACCAGCAGCGGAGATCCTTAAAGGATCTCCGGAAGACTATAAAATCCCTGATGCGCTACGCTCATCAGGCATCCAGGCCTACCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTTGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGcc < 2:108898/233‑1 (MQ=255) tCGATCAATGTCACCGGCGGTCAGGTGA‑TGGTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCa > 1:127491/1‑250 (MQ=255) gtccccggggggcAGGTGA‑TGTTCTAATCAACAGCGGAGATCAATTAAGGATCTCCGTGAGAATATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGtt < 1:131481/239‑1 (MQ=255) gTCACCGGCGGTAAGGTGA‑TGTTCTGAACAAAAGCGGAGATCCATTAAGGATCTCCGTGAGCATAAAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGt < 1:172970/250‑1 (MQ=255) gcccgggcggTCGGGGGA‑TGTTGTGATAACCAGCGGAGACCAATTAAGGTTCTCGGTGAGCATAGAGAATGCCGGATGCCCTACGCTAACGAGTCATAGAGTCTTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATGTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGtt < 2:418919/245‑1 (MQ=255) aGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGCCTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGTTGGCGTGGGCCGtt > 1:264734/1‑251 (MQ=255) ggTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGCTGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGTTGGCGTGGGCCGTTc > 1:85809/1‑251 (MQ=255) | GAGTACGGCATTACGGTGCATTCACTGATGCTCGGTAACCTGCTGAAATCGCCGATGTTCCAGTCACTGTTGCCACAATACGCGACCAAGCTGGGTATCAAACCGGATCAAGTCGAGCAGTATT‑ACATCGACAAAGTACCGCTCAAACGCGGCTGCGATTATCAAGATGTGCTGAATATGCTGCTGTTCTA‑CGCCAGTCCTAAGGCGTCGTACTG‑CACCGGACAGTCGATCAATGTCACCGGCGGTCAGGTGA‑TGTTCTGATCAACAGCGGAGATCCATTAAGGATCTCCGTGAGACTATAGAATGCCTGATGCGCTACGCTCATCAGGCATACAGGACTTCCGCCACTACATTAAGGAAAAGTTATGGTATCCGCACTCATCACCGTCGCCGTTATCGCCTGGTGTGCGCAACTGGCC‑TTAGGCGGCTGGCAAATTTCTCGTTTTAACCGTGCCTTCGACACACTATGCCAGCAAGGGCGGGTTGGCGTGGGCCGTTC > NC_000913/2828256‑2828754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |