Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,465,513	0	C	G	25.6%	74.8 / 12.7	39	G71G (GGC→GGG)	yfdC	inner membrane protein YfdC
Reads supporting (aligned to +/- strand):  ref base C (6/23);  new base G (10/0);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 1.26e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGTGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTGCTGCTGGGTAATATTCTCGGGACAGGTATTGCG  >  NC_000913/2465276‑2465762                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                              acgagCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTTATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGGGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCCTGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATTGGCTCTTCGGTAC‑Tgg                                                                                                                                                                                                                                                 <  1:439607/247‑1 (MQ=255) aCGTGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑Tgg                                                                                                                                                                                                                                                 <  1:27039/251‑1 (MQ=255)    tGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGGGTGTCGAGGGGGGCTGCGTTAA‑Tgggc                                                                                                                                                                                                                                               >  1:160368/1‑248 (MQ=255)            cAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGat                                                                                                                                                                                                                                       <  1:216957/250‑1 (MQ=255)                                       aaaTTGATCAACACAGCGACGAAAGTGAAGTGGAGAGCGAAGAAAAAGAGTGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGGCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGtt                                                                                                                                                                                                                                                       <  2:480049/208‑1 (MQ=255)                                        aaTTGATCAACACAGCGAAGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTATGTCGAGGGGGGCTTCGTTAC‑TGGCAAAAGGGATATTTTAAGTCggaatgggaggggggtggg                                                                                                                                                                                                          >  1:496308/1‑233 (MQ=255)                                                          cgaAATGGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGAGGAAGACCGACTCCCCACCCGGGCGATGGCAATTCATGAGCATATCCGGCAGGATGGTTAAAAGGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCGATTGTGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACGGGAAGGTGTGCCGGGCAGctt                                                                                                                                                                                                   <  1:248401/240‑1 (MQ=255)                                                                  aaGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGt                                                                                                                                                                               <  2:394678/251‑1 (MQ=255)                                                                   aGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCGTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGtt                                                                                                                                                                              <  1:291709/251‑1 (MQ=255)                                                                    gTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCTAGTCGAACTGGGAGGGGGGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGttt                                                                                                                                                                             >  1:72884/1‑250 (MQ=255)                                                                                gaaAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGCCCGCCTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGttt                                                                                                                                                                <  2:153734/251‑1 (MQ=255)                                                                                   aaaGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTTAAGGTGGGGGCTTTGTTAC‑TGGGCAAAGGGGCATTTTCAGTCGGAATGGAAAGGGGGCCGGGGGGGTTCTTTTTGGGGAATCTC‑GGGTATACCTTTTGGttttt                                                                                                                                                              >  1:370364/1‑250 (MQ=255)                                                                                    aaGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACGCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCGTTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTttatt                                                                                                                                                            <  1:315459/251‑1 (MQ=255)                                                                                      gagCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTTTTGCTGGGCGCTTCTTTGC‑CGGGCAAAGGGGTATTTTAAGTAGACCTGGGAGGAGTGCCGGGGCGCTTCTTTCTTGGGAATCTC‑GGTTTTACCTTT‑TGttttttttt                                                                                                                                                          >  1:315649/1‑246 (MQ=255)                                                                                              cAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAg                                                                                                                                                                                                      <  1:110591/201‑1 (MQ=255)                                                                                               aaaaaaaTAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAAtctc                                                                                                                                                                                   <  1:337626/219‑1 (MQ=255)                                                                                                      tagaaatagaTGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTATGTGGATGGGGGCTTTGTTAC‑TGGCGAAAGGGAGATTTTAAGTCGAACTGGAAGGGGTGCCGGGCAGCTTCTTGCTGGGGAATCTT‑GGGTATAACTTT‑GGTTTTTTTTTCGTCATTATTGCCCGc                                                                                                                                          >  1:559740/1‑251 (MQ=255)                                                                                                              gaTGAAGACCGACCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTGCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCATTATGGCCCGCCAGCAatt                                                                                                                                  <  1:4579/251‑1 (MQ=255)                                                                                                               aTGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGCTGGGGGGTTCGTTTC‑TGGGAAAAGGGGGATTTCAAGTCGGACTGGAAGGGGGGCCGGGCAGGTTCTTTCGGGGGAAGCTC‑GGTTTTACCTTTTGGTTTT‑TTTTTTTCCTTTTGGCCCGCCAGCAattt                                                                                                                                 >  1:308086/1‑250 (MQ=255)                                                                                                                    gACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGAACGTCCATGGGCGCCACTTTCA‑TCGCAAAAAGGGGATTTCAAGCAGAAATTGAAGGAGTGGCGGGCAACTTCTTGCTGGAGAATCCCCGGTT‑TTCCTTT‑AGTTTTATTTTCCTCATTATTGgccgcccgacatttttttt                                                                                                                            >  1:69984/1‑238 (MQ=255)                                                                                                                     cccGACCCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTAcc                                                                                                                           <  2:308086/250‑1 (MQ=255)                                                                                                                       cGACTCCCCTCCCGGGCTATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGGAGGGGGCTTCGTTAC‑TGGGAAAAGGGGTATTTCAAGTCGAACTGGGAGGTGGGCCGGGCAGCTTCTTGTTGGAGGATATC‑GGGTATTCCTTT‑TGTTTTTTTTTTGTCATTATTGCCCGCCCGCAAATATTTTCCgc                                                                                                                         >  1:501488/1‑250 (MQ=255)                                                                                                                        gACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCAtt                                                                                                                                                   <  2:482878/224‑1 (MQ=255)                                                                                                                         aCTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGGGTGTCGATGGGCGCGTCGTTAC‑TGGCAAAAGGGATATTTTAAGTCGAACTGGAAGGGGTGGCGGGCAGCTTCTTTCTGGGGAATTTC‑GGGTATTACTTT‑GGTTTTTTTATCGTCCTTTTTGGCCGCCCGCAATTTTTTTCCgca                                                                                                                        >  1:418562/1‑248 (MQ=255)                                                                                                                                          tGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTTGGTGGGGGCTTCCTTACCTGGCCAAGGGAATTTTTCA‑GTCCAACTGGGAGGGGTGGCGGGCCGCTTTTTGGTTGGGAATGTC‑GCATTTAACTTT‑GGTTTTATTATCGTCTATATGGCCCCCCCGCAATTAtttttccaaaataaacggactccggg                                                                                                       >  1:107423/1‑227 (MQ=255)                                                                                                                                               aTTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGGTATTTTAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTTCTGGGGGATCTC‑GGGTATACCTTT‑TGTTTTTTTATCGTCATTATTGCCCGCCCGCAATTATTTACCGCAAAAACCCTGACTGCGG‑GACTAcc                                                                                                >  1:6467/1‑251 (MQ=255)                                                                                                                                                             ccGCCAGGATGGTGAAAAAGAGCTGGAACGCGCCGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCGGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCTAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGATATACCTTT‑GTTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTACCCTTCATGCAAAAAc                                                                                  <  2:551813/251‑1 (MQ=255)                                                                                                                                                              cGCCAGGAGGGTGAAAAAGACCTGGAACGCGACGCAATTGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCATCTTTAC‑TGGCAAAAGGCATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTTCTGGGGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGGCATTATGGCCCGCCAGCAATTGTCTACCGAAAGTACCGTGACTGCGG‑TACTACCCGCCATGGAAAAAcc                                                                                 <  2:279421/251‑1 (MQ=255)                                                                                                                                                                  aGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGGGCTTCGTTAC‑TGGCAAAAGGGGTATTTTAAGTCGAAATGGGAGGTGTGCCGGGCAGCTTCTTTCTGGGGAATCTC‑GGTTATACCTTT‑GGTTTTTTTATCGTCATTATGGCCCGCCCGCAATTATTTACCGAAAATACCGTGACTGCGG‑TAATACCCCTCATGCAAAAACCGAc                                                                              >  1:321213/1‑250 (MQ=255)                                                                                                                                                                   ggATGGTGAAAAAGAGCGGGAACGCGACGCAAGGGCGCTACTGTGGTCATCCATTGCGGCGGGTCGGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTACCCGTCATGCAAAAACCGACaa                                                                            <  1:222/251‑1 (MQ=255)                                                                                                                                                                       ggTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTACCCGTCATGCAAAAACCGACAATGAg                                                                        <  1:112124/251‑1 (MQ=255)                                                                                                                                                                        gTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTAACGATGGGGGCTTCGTTTA‑TAGCCAAAGGGATATTTCAACTCCAACTGGAAAGGGTGCCCGGGAGCTTCTTTCTGGGGAATCTC‑GGGTCTACCTTT‑GGGTTTTTTTTAGTCCTTATTGCCCGCCCGCCATTATTTTTCGAAAATAACGTGACTGCGG‑TGCTACCCCTCATGCaaaaaaccccaaagagg                                                                       >  1:261255/1‑239 (MQ=255)                                                                                                                                                                            aaaaGAGCGGGAACGCGACGCAAGGGCGCGACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTG‑GGTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTACCCGTCATGCAAAAACCGACAATGAGCAACg                                                                   <  2:501488/251‑1 (MQ=255)                                                                                                                                                                                             aCGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTTGAAGGGCGCCTCGTTTC‑TGGGGAAAGGGGTATTTTAAGGAGAACTGGGAGGGGTGGCCGGCCGCTTTTTGCTGGGGAATCTC‑GGTTATACCTTT‑GTTTTTATTTTCGTCATTTTGGCCCCCCAGCAAATATTTTACGCAAATACCCTGACTGCGG‑TGCTACCCGGCCTGCAAAAACCGACAAAAAGCAACATCGGCTTACTTATACgg                                                  >  2:170531/1‑251 (MQ=255)                                                                                                                                                                                                  aTGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTAGATGGGGGCTTTGCTAC‑TGGCAAAAAGGATATTTTAAGTCGGACTGGAAGGGGGGCCGGGCCGATTCTTGCTGGAGAATCTC‑GGGTATACCTTT‑TGTTTTTTTATCGTCATTTTGGCCCGCCAGCAATTATTTTCCGAAAATAACCTGACTGCGGGAACTACC‑GCCATGCAAAAAACGACAATTAGCAACCTCGGCTTTCTTATACGGtttat                                             >  1:53768/1‑248 (MQ=255)                                                                                                                                                                                                          atgggggTCAGCCATTGGGGCGGGTCGGTCGAGGGGCGCTTCGTTAC‑TGGCAAAAGGGATTTTTCAAGTCGAACTGGAAGGTTGGCCGGGCATCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑TGTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGT‑TACTACCCGTCATGCAAAAACCGACAATGAGCAACTTCGGCTTACTTATACGGTTATGGGGcgtcg                                     <  2:496308/246‑1 (MQ=255)                                                                                                                                                                                                                     ccATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTGCTGCTGGGt                          <  1:53879/251‑1 (MQ=255)                                                                                                                                                                                                                             gcggGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTGCTGCTGGGTAATATtct                  <  2:418562/251‑1 (MQ=255)                                                                                                                                                                                                                             gcggGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTCCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCCC‑GGTTATACCGTT‑GTTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTTCCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTGCTGCTGGGTAATATtct                  <  2:370364/251‑1 (MQ=255)                                                                                                                                                                                                                                             gcgcTTCGTTAC‑TGGCAAAAGGGATATGTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCAGTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATTGGGCGTCGTGCTGCTGGGTAATATTCTCGGGACAGGTATTgcg  <  1:516679/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                              ACGTGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCCCGGGCGATGGCAATTCATGAGCATATCCGCCAGGATGGTGAAAAAGAGCTGGAACGCGACGCAATGGCGCTACTGTGGTCAGCCATTGCGGCGGGTCTGTCGATGGGCGCTTCGTTAC‑TGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTC‑GGTTATACCTTT‑GGTTTTATTATCGTCATTATGGCCCGCCAGCAATTATTTACCGAAAATACCGTGACTGCGG‑TACTACCCGTCATGCAAAAACCGACAATGAGCAACGTCGGCTTACTTATACGGTTATGGGGCGTCGTGCTGCTGGGTAATATTCTCGGGACAGGTATTGCG  >  NC_000913/2465276‑2465762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑