| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 266,049 | 0 | A | C | 28.8% | 70.3 / 11.6 | 52 | G13G (GGT→GGG) | ykfB | protein YkfB |
| Reads supporting (aligned to +/- strand): ref base A (24/13); new base C (1/14); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCC‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCC > NC_000913/265800‑266290 | aatGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCC‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTAGTCCGTATTGCCCTGACC‑GTAACCC‑GGCTGCCAGAACCCCGGG‑ATAGCAGGGGCGTTAAAG‑TTCGCGAGCA‑GT‑aca < 2:386778/250‑1 (MQ=255) atGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCC‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACAcc < 1:549529/251‑1 (MQ=255) tGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTCTCGTCATCGTACGAACTGACCACCTTATCAACGGCACGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCC‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGACTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACAccc > 1:534880/1‑251 (MQ=255) gTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAATCCATGCTGGTCC‑GGGTTCCCTGTGCAGTGGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTAGTCCGTATTGCCCTGACC‑GTAACCC‑TGCGGTCAGAACCCGGGG‑ATAGCAGATGCATTAAAG‑CTCGCGAGCA‑GT‑ACAccc < 1:210638/250‑1 (MQ=255) cATCACTACGGTGATAGTAGTGACGTCATCGTGCGAACTAACCATATTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTAC‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTACGCTGGCTTCGGTCACGATGTATTCCGTATTGCACTGACC‑GTAACCC‑TGCTGCCAGAACACCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGAc > 1:84494/1‑251 (MQ=255) aCGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCC‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTg > 2:491294/1‑250 (MQ=255) aaTAGTAGTGTCTTCATCGTGCGAACTGCCCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATTCTGATAAAACCCATTCTGTTCC‑GGTTTCCCTGTGAAGTTGATGGTAA‑ACGTCTTTCCGCGGCCTTCGGTCACGCTGTATTCCGTATTGCCCTGCCC‑GTACCCC‑TGCTGCCAGA‑CCCCGGGTATAGCAGAGGATTTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGCCTTAGTGAAAGt < 1:407427/249‑1 (MQ=255) gggcgtaaTCGTGCGACCTGACCATCTTTTCATGGGCAAGGGTAAGAAAGACTGAATGCTAAAAGAAACCATTCTGGCCC‑GGGTTCCGTTTGCAGTGGATGGAAA‑CCTTCTTTCCGCGGGCTTCGTTCACGCTGTATTCCGGTTTGCCCTGACC‑GAAACCC‑TGCTGCCAGACCCCCGGT‑ATAGCAGGGGGATTAAAG‑CTCGCGGGAG‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCAtt < 1:284608/244‑1 (MQ=255) cATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGCAAGACTGAATGCTGATCGCAACCATTCTGGTCC‑GGGATCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGtc > 2:49857/1‑251 (MQ=255) gCGACCTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAACCCATTCTGGTCC‑GGGTTCCCTGTGCAGTTGATGGAAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCtt < 1:221843/250‑1 (MQ=255) accggACCATCTGGCCACCGGAAAGGGAAAGAAAGACTGAATGCGGATAGAACCCATTCTGGTCG‑GGGTTCCCTGTGCAGTTGATGGAAA‑ACGTCTTCCCGCGGGCTGCGGTCACGCTGTATCCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGACCCCGGGG‑ATAGCAGAGGCATAAAAT‑CTCGCGAGCA‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTc < 1:226649/247‑1 (MQ=255) aaCTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCC‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTTTTCCTTATTGCCTTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTGGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTc < 1:509346/251‑1 (MQ=255) tGTCATCGGAAAGGGTAAGAAAGACTGAAGGCGGATAGAAACCATTCTGGTCC‑GGGTTCCCTGTGAAGTTGATGGAAA‑CCGTCTTCCCGCTGGCTTCGGTCCCGCTGTATTCCGTAGTGCCCTGACC‑GAAACCC‑GGCTGCAAAACCCCCGGG‑ATAGCAGAGCCATAAAAG‑CTCGCGAGAA‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTAt < 1:364430/249‑1 (MQ=255) ggCAGGGGTAAAAAAGCCTGAATGCTGATAGAA‑CCCTTCCGGTCCCGGGTTCCCTTTGCATTTGATGGTAG‑ACGTTTTGCCGTGGGCTTCGGTCCCGCTGTTTCCCTTTTTGCCCTGCCG‑GTACCCT‑TGCCGACAGACCCCCGGA‑ATAGCAGTGGCATTAAAG‑CTCGC‑AGCAAGT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGGATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTg < 1:64444/251‑1 (MQ=255) gaaagaCTGAATGCTGATAGAAACCATTCTGGTCC‑GGGTACCATGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCg > 1:499586/1‑250 (MQ=255) agaCTGAATGCTGATAGAAACCATTCTGGTCC‑GGGTTCCCTGTGAAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGAGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAggg > 1:292860/1‑251 (MQ=255) gATAGAAACCATTCTGGTCC‑GGGTTCCCGGTGCAGTTGATGGAAA‑ACGTCTTCCCGCTGGCTTCGGTCCCGCTGTATTCCTTATTGCCCTGCCC‑TTAACCC‑TGCTGCCAGACCCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACCCCCGCCAGCATAAACCGa < 1:520977/170‑1 (MQ=255) gATAGAAACCATTCTGGTCA‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCTGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGa > 2:520977/1‑170 (MQ=255) gAAACCATTCTGTCCC‑GGTTTCCCTGTGCAGTTGTTGGTAA‑CCGTCTTCCCGCTGGCTTCGGTCGCGTTGTTTCCCTTATTGCCTTGCCC‑GTACCCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGCAATTAAAG‑CTCGCGAG‑A‑GTTCCACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGcccc < 1:320154/251‑1 (MQ=255) ccATTCTGGTCC‑GGTTTCCCTGTGCAGTTGATGGAAA‑ACGTCTTTCCGCTGGCTGGGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTACCCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CCCGCGAGAA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCa < 1:491294/251‑1 (MQ=255) ccATCCTGGTCG‑GGGTTCCCGGGGCTGTTG‑GGGTAACACGTCTTCCGGCGGTCTTCGGTCACGTTGTATTCCGTAT‑GCCCCGGCC‑GGAACCCCTGCTACCAGACCCCGGGG‑ATAGCAGAGTCATTAAAG‑CCCGCGAGCG‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCa < 2:125274/251‑1 (MQ=255) cgggccc‑GGGTCCCCTTGGCATTTGATGGAAA‑CCGTCTTTCCGCTGGCTTCGGTCCCGCTGTATCCCGTATTCCCCTGACC‑GTACCCC‑GGCTGCCAGAACCCCGGG‑ATAGCAGAGGAATTAAAG‑CCCGCGAGCA‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGttt < 1:366793/245‑1 (MQ=255) gTCC‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCg > 1:289993/1‑251 (MQ=255) ggggtccttgtgCAGTTGATGGTAA‑ACTTCTTTCCGCTGGCTTCGGTCACGCTGTATCCCGTATTGCCCTGACC‑GGACCCC‑GGCTCCCAGAACCCGGGG‑AAGGCAGAGGCATTAAAG‑CTCGCTGGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCGGATTGTCAGTGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCAt < 1:49857/243‑1 (MQ=255) ggTTCCTTGTGCTGTTGATGGTAA‑CCGTCTTTCCGCGGGCTTCGGTCCCGCTGTATTCCGTTTTGCCCTGACC‑GTAACCC‑TGCTGCCAGACCCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCG‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATg < 2:21664/251‑1 (MQ=255) gTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGa > 1:364798/1‑156 (MQ=255) ttCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATggg > 2:52151/1‑251 (MQ=255) ttCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATggg > 1:215868/1‑251 (MQ=255) ttCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGAATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATggg > 2:249691/1‑251 (MQ=255) cccTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCGCGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCg > 2:406321/1‑251 (MQ=255) cccTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCg > 2:440523/1‑251 (MQ=255) tgCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTc > 1:314351/1‑251 (MQ=255) gTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTgg > 1:415096/1‑251 (MQ=255) tttCCGCTGGCTTCGGTCCCGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGtat > 1:482028/1‑251 (MQ=255) ccgctgctttCGGTCACGCTGTCTTCCGTATTGCCCTGACC‑GTACCCC‑TGCTGCCAGACCCCCGGG‑ATGGCAGAGGATTTAAGG‑CTCGCGAGCA‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTTCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACg < 1:112957/243‑1 (MQ=255) gcggccACGCTGTATCCCTTATTGCCC‑GACCCGTAACCC‑TGCTGCCAGACCCCCGGG‑AAAGAAGAGGAATTAAAG‑CTCGCGAGCA‑GT‑CCACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTc < 1:118580/245‑1 (MQ=255) ggTCACGCGGTATCCCGTATCGCCCAGACC‑GTAACCC‑TGCTGCCA‑AACCCCGGGGATAACAGGGGCATTAAAG‑CGCGCGAGCA‑GT‑CCCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACTGGTCCGTCAcc < 2:59327/251‑1 (MQ=255) gTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCAcct > 1:482487/1‑251 (MQ=255) ccgcggTATTCCGTATTGCCCTGCCC‑GTAACCC‑TGCTGCCAGACCCCGGGG‑ATAGCAGAGGCATAAAAG‑CTCGCGAGCA‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCAcctcct < 2:430574/246‑1 (MQ=255) cgcgttatcccGTATTGCCCTGACC‑GTAACCC‑GGCTGCCAGACCCCCGGG‑ATAGCAGAGACATAAAAG‑CCCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCACTCTTGTCGCCGTTGTCGTTTTTTTATTCCTGATTGGCAGGGTTCGAGGGTATCAGTAGCTTCCCCATCGGTTTGCCGTCAGGTGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTg < 1:502801/242‑1 (MQ=255) ccgaacagaacccgtgcggccacacccccGGG‑ATAGCAGAGGAATAAAGG‑CACGCGAGCA‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGtt < 2:160402/226‑1 (MQ=255) c‑tgctgACAGACCCCCGGG‑ATAGCAGAGGCATTACAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATCAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTACTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTAATCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTACAGTTTGATGTTATCGGCAATacac > 2:239116/1‑251 (MQ=255) cgtctgcCATACCCCCGGG‑AGAGGAGAGGCATTAAGG‑CTCGCTGGCT‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATacac < 1:106367/248‑1 (MQ=255) gctgcCAGACCCCCGGG‑ATAGCAGAGGC‑TTAAAGGCTCGAGAGGA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTGTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATacac < 1:52151/249‑1 (MQ=255) aGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGTAAGCATAAACCGACTTAGTGAAACTATCGTCTTTCTTGCCTCTTTTGTCGTTGTTTTATTACTGATTGACCGGGTTCGAGGGTATCAGTAGCTGTCCCATCAGTTTGCCGTCCTGGGCGTACTCAAAGTATTTTTCTTTGGTGTACGGGTCCGTCACCACCTGGTATTCCAGTTTGATGTTATAGGCAATACACAGTGCAtt > 1:550723/1‑251 (MQ=255) aTAGCAGAGGAATTAAGG‑CTCGGGAGCA‑GT‑TCACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCGGGGATTCCAGTGTGATGTTAGCGGCAATACACAGCGCATTCATCAGTGGCt < 1:73606/251‑1 (MQ=255) agcagaggcattaaccccggcggtcA‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCGTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTgg < 2:215868/227‑1 (MQ=255) agagGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACg > 2:85602/1‑251 (MQ=255) cATTAAAG‑CCCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGtttt < 1:421756/251‑1 (MQ=255) gcgAGCA‑GT‑ACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCatat > 2:77126/1‑251 (MQ=255) gAGCA‑GT‑ACACCCGCCAGCATAAAACGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATcc > 1:126018/1‑251 (MQ=255) aGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCACTACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTgactatcc > 2:279/1‑243 (MQ=255) a‑GT‑ACACCCGCCAGCATACACCGACTTAGTGAAAGTATTGTCATTCCTGTATCCTTTGTCGTTGTTTTATTCCTGCTTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTAtt > 2:503558/1‑143 (MQ=255) | AATGTACTGCTGGTGATCCATCACTACGGTGATAGTAGTGTCGTCATCGTGCGAACTGACCATCTTGTCATCGGCAAGGGTAAGAAAGACTGAATGCTGATAGAAACCATTCTGGTCC‑GGGTTCCCTGTGCAGTTGATGGTAA‑ACGTCTTTCCGCTGGCTTCGGTCACGCTGTATTCCGTATTGCCCTGACC‑GTAACCC‑TGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAG‑CTCGCGAGCA‑GT‑ACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAGGGTATCAGTAGCTGCCCCATCAGTTTGCCGTCATGGGCGTACTCAAAGTATTTTTCTTTGGTATACGGGTCCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCC > NC_000913/265800‑266290 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |