| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 119,396 | 0 | C | T | 27.1% | 121.0 / 18.5 | 70 | S39F (TCT→TTT) | ampE | protein AmpE |
| Reads supporting (aligned to +/- strand): ref base C (12/39); new base T (19/0); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCG > NC_000913/119152‑119640 | gattgattGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑g < 1:407713/250‑1 (MQ=255) gattgCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGgcgc < 1:569386/251‑1 (MQ=255) tgCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGCTCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGCGCGCCTGTTTATGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAAAAGTTTTTTCTC‑GGGCGCACg > 2:355632/1‑251 (MQ=255) gCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGCG‑GAACATTTTTTTTTC‑gggggcgcgt > 2:69472/1‑244 (MQ=255) aTCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTGCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCGGTTTAAGTTGTGCGAGCACTGGCAGCTTGATCAGCGTCTTGAAGCGTTCTTTCGACGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGtt < 2:553028/249‑1 (MQ=255) ggATATCGCTAAAAACATGACGGGCCATTGTGATATTGCTCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTTGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCTGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCAt < 1:80606/251‑1 (MQ=255) atCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGAc < 1:166980/250‑1 (MQ=255) gCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCatt < 1:25689/251‑1 (MQ=255) cTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTTTT‑GGGGGCACGTTA‑GGCCTGAcccttt > 1:165861/1‑247 (MQ=255) aaaaaCATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTGGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCattatt < 1:93970/251‑1 (MQ=255) aaaaaCAGGTCGTGCCATTGTGATATTTCGCCTGATCGGAAAACCGAGCCCGGTCCTGCATGTGATTAGGCACGGTTTCTTGTGCTGGTCAGCAAGTAGACAACATGACGCTATTTACAAGCTTTCTGGTTTTAATGTTCGATCTCCTTTTTAAGATGCGCGAGCACTGGCAGCTTGAGCATCGGCTGGAAGGGTTGTTTCGGCTGGTT‑AAGCATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCattat < 1:242285/250‑1 (MQ=255) aaCATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGGCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGTTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGGAGCGTTCTTTCGGCGGGTG‑AAACATGTTTCTCTC‑GGGCTCACGTTA‑GGCATGACCATTATTGCg < 2:547031/251‑1 (MQ=255) ccATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑gg < 2:17441/221‑1 (MQ=255) cATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAATATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑At < 1:187514/251‑1 (MQ=255) gCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTAGTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGATGCGTTCTTTCGGCGGGTG‑AGACACTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGa < 1:534603/251‑1 (MQ=255) aTTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑CAACATTTTTTTCTC‑GCGCGCCCGCTA‑TGCATGCCCATTATTTCG‑CTGGGGGGGACtt > 2:264599/1‑251 (MQ=255) atatTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAC‑TTTTTCTTTC‑CGGCGGGCGTTT‑GGCCTGACCATTATTGCG‑CTGGGCGTGGCTTTTTTat > 1:214580/1‑250 (MQ=255) gcgcCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTa < 1:432258/251‑1 (MQ=255) gagcCGGATCGGAAAACCGAGCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTGCTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑tt < 2:5414/248‑1 (MQ=255) cGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTg < 1:355632/251‑1 (MQ=255) ggAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGc < 1:457783/251‑1 (MQ=255) ggAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGCCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGAGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTTTT‑GGGGGCCCGTTATGCCAGGACC‑TTTTTGCG‑TTGGGCGGGGCTTTTTTTCTG‑TTTAGCGCATTGc > 1:365729/1‑251 (MQ=255) aaCCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCTC‑GGGCGCCCGTTA‑AGCCAGACCATTATTGTG‑CTGGGGGGGGCTTTTTTACTG‑TTACGCGCATTGCAggg > 1:321504/1‑251 (MQ=255) gATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTAc < 2:141135/228‑1 (MQ=255) gATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCTC‑GGGCGCACGTTA‑GGCCTGACCCTTATTGCG‑ATGGGCGGGGCTTTTTTAc > 1:141135/1‑228 (MQ=255) cccGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCGGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTGACTGGTGTTAAGTTTCGAGCGCCTGGTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTAGTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTg < 1:341514/251‑1 (MQ=255) ccGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAAAATTTTTCTCTC‑GGGCGGACGTTA‑GGTCTGACCATTTTTGCG‑ATGGGCGGGCCTTTTTTACTG‑TTTCGCGCATTGCCGGGGGAATTGt > 1:302526/1‑251 (MQ=255) ccTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCTGGTG‑AAACATTTTTCTCTC‑GGGTGCACTGTA‑GGCAGGACCATTATTGCG‑ATTGGCTTGACTTTATTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAAc < 1:50158/251‑1 (MQ=255) ttGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTccc < 1:476688/250‑1 (MQ=255) tGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCTC‑GGGCGCCCGTTA‑GGCATGACCATTATTGCG‑ATGGGGGGGGCTTTTTTACTG‑TTACGCCCATTGCCGGGGGTATTGTT‑CAACGTTCCCACGCtc > 1:204847/1‑250 (MQ=255) aCGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑GACCATTTTTTTCTC‑TGGCGGCCGGTA‑AGCATGGACATTTTTGCG‑GTGGGCGTGACTTTTTTTTTGGTTA‑GCGCATTGCAGGGGGTATTTTT‑CAACGTTcccccggtcctggg > 1:503709/1‑240 (MQ=255) tCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGGGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATGGTT‑CAACGTTCCCACGCTACTGGTGTGgc < 2:165861/250‑1 (MQ=255) ggTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTT‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTgg > 1:412724/1‑251 (MQ=255) gTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGCTCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTC‑GGGCGCCCGTTA‑GGCCTGACCATTATTGCG‑ATGGGCGTGGCTTTTTTTCTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTTCCCCGCCACTGGTGTGGCTGCTGAtttgt > 1:73418/1‑248 (MQ=255) cAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTAGAGCGCCTGTTTAAGTTGGGCGAGCACTGACAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑CAACATTTTTTTCTC‑AGGCGCACCTTA‑GGGACGGCCATTATTGCG‑ATGGGCGGGGCTTTTTTATTG‑TTTCGAGCATTGCAGGGGGGATTGTTTCAAC‑TTTCCACACCAATGGTGTGgctggtgctttggttgct > 1:565691/1‑237 (MQ=255) aacaacaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTGCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCTCGTTA‑GGCATGACCATTATGGCG‑ATGGGCGTGACTTTTTTGCTG‑TTACGCGCATTGCAGGGAGTATGGTT‑CAACGTGCCCACGCTACTGGTGTGGCTGCTGATGGGTTTGCTG‑TGTAt < 2:503709/250‑1 (MQ=255) acaacaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCGGATTGGTTTGCGG‑TGTAtt < 1:443622/251‑1 (MQ=255) cTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGGCTTTTTTACTG‑TTTCGCGCATTGCAGGGAGGATTGTT‑CAAAGTTCCCACGCTACTGGGGTGGCTGCTGATTGGTTTTCTG‑TGTATTGGCGCAGGTa > 1:325253/1‑250 (MQ=255) aCAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑TAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACTCGCATTGCAGGGAGTATTGGG‑CAACGTTCCCACGCTACTGGTGTGGCGGCTGATTGGTGTGCTG‑GGTAGTGGCGCATGTAAAGTTCg < 2:487644/251‑1 (MQ=255) cAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCAATGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAAACTTTTTCTCTC‑CGGCGCACGGTT‑GGTATGACCATTATTTCG‑ATGGGCGAGAATTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGCTACCACGCTACTGGTGTGGGTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGttagt > 1:290347/1‑248 (MQ=255) aCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑GAAAATTTTTCTCTCCGGGGGC‑CGGTT‑GGCATTGCCATTTTTTCGGATGG‑CGTGACTTTTTTTCTG‑TTAAGCGCCTTGTAGGGAGGATTTTT‑CAACGTTCCCACGCTACTGGGGGGGCTTGTGATTGGGTTTCTG‑GGTATTGGTGCAGGGAAAgatacttt > 1:15231/1‑244 (MQ=255) aCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTC‑GGGCGCACGTTA‑GGCATGACCCTTTTTGCG‑GTGGGCGTGACTTTTTTTCTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCCCGCTACTGGTGGGGCTGCTGTTTTGTTTGCTG‑TGTATTGGCGCAGGGAAAGTTCGTCt > 1:460594/1‑251 (MQ=255) cctgaCTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCtt < 2:426230/247‑1 (MQ=255) cTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGAG‑AAACATTTTTTTCTC‑GGGCGCACGGTA‑GGCATGACCCTTATTGCG‑ATGGGCGTGACTTTTTTTCTT‑TTTCGCGCATTGCAGGGGGTATTGTT‑CAACGTTCCCACGCCACTGGTGTGGCTGCTGGTTGGTTTTCTT‑TGTTTTTGCGCAGGTAAAGTTCGTCttt > 1:236513/1‑250 (MQ=255) aCTGGTGTTAATTTTCGAGCTCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCGTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTT‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGAGTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCAtt < 1:523392/251‑1 (MQ=255) ggTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATc < 1:454251/251‑1 (MQ=255) ggTGTTAATGTTCGAGCGCCTGTTGAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCTCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTGACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTGCCCACGCTACTTGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGGCTTCATTa < 2:550933/249‑1 (MQ=255) ttttCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑GAACATTTTTTTCTC‑CGGCGCACGGTA‑GGCGTGGCCCCTATTTCG‑GAGGGGGTGGCTTTTTTTCTG‑TTACGCGCCTTGCAGGGGGGATTTTT‑TAACCTTCCCCCGCTACTGGGGGGGGTGCTGATTGGTTTTTTG‑TGGTTTTGCGCCGGGAAAGTTCGTCTTctttttatttctttt > 2:366406/1‑237 (MQ=255) ttCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTTCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATc < 1:69472/250‑1 (MQ=255) cGAGCGCCTGGTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGGCTTCATTATCATGCTTATCTg < 2:302526/250‑1 (MQ=255) gAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGCAGCGTTCTTTCGGCGGGGG‑AAACCTTTTTTTCTC‑GGGCGCACGCTA‑GGCATGACCATTTTTGCG‑ATGGGAGGGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGGATTTTT‑CAAAGTTCCCACGCTAATGGGGTGGCTGCTGATTGGTTTTCTG‑TGTATTGGGGCCGGTAAAGTTTGTCTTTATTTTTATGCTTATCttac > 1:54045/1‑248 (MQ=255) gAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGgc < 2:14214/213‑1 (MQ=255) cTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCTC‑GGGCGCACCGTA‑GGCATGACCATTATTGCG‑GCGGGCGGGACTTTTTTACTG‑TTACGCGCATTGCAGGGGGGATTGTT‑CAACGTTCCCACGCTACTGGGGTGGCTGCTGGTTGGGTTGCTG‑GGTATTGGGGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAgctgc > 1:19008/1‑251 (MQ=255) cTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAAAATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGGGTGGGTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGGAAAGTTCGTCTTTCTTATCATGCTTATCTGCAAgctgc > 1:447292/1‑251 (MQ=255) tAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATGTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCAGATTGGTTTGCTG‑TGTATTTGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCa < 1:515951/250‑1 (MQ=255) aaGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCAc < 1:496874/250‑1 (MQ=255) gTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTTTCTC‑GGGCGCACCGTA‑GGCATGACCATTATTTCG‑AGGGGCGTGACTTTTTTTCTG‑TTTCGCGCATTGCAGGGAGGATTTTT‑CAACGTTTCCACGCTACTGGTGGGGCTGCTGATTGGTTTGCTG‑TGTGTTTGCGCCGGGAAAGTTCGTCTTCATTATCCTGCTTATCTTACAGCTGCTTCCCGt > 1:316783/1‑250 (MQ=255) gTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCGC‑GGGCGCACGTTA‑GGCATGACCATTAGTGCG‑AGGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGATTATTGTT‑CAACGTTCCCACGCTACTGGTGTGgctgct < 1:36002/177‑1 (MQ=255) aGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑GAAAATTTTTTTTTC‑GGGCGCACGTTA‑GGGCTTACCCTTTTTGTG‑GTGGGGGTGGCTTTTTTTCTG‑TTACGCGCATTGCAGGGGGTATTTTT‑CAACGTTTCCACGCTACTGGTGTGGGTGCTGATTGGGTTGCTG‑TGGATTGGGGCCGGGAAAGGTCGTCTTTATTATTCTGCTTTTCTggtaggtgcttcccggtatgat > 2:59773/1‑227 (MQ=255) aGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTTTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTTCTG‑TTACGCGCATTGCAGGGAGGATTGTT‑CAACGTTCCCCCGCTACTGGTGTGGCTGCTGATTGGGTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGc > 1:339809/1‑251 (MQ=255) gCACTGGCAGCTTTATCATCGTCCTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCC‑ATGGGCGTGACCTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGcc < 1:418162/251‑1 (MQ=255) tGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGG‑AAACATTTTTCTCTC‑GGGCGCACGGTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCTGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGTTTGGTTTGCTG‑TGTATTGGCGCAGGTAACGTTCGTCTTCATTTTCATGCTTATCTGACAGCTGGTTCACGTAATGATAGCCATGc > 1:416384/1‑251 (MQ=255) aGCTTGATCATCGGCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGt < 2:339809/251‑1 (MQ=255) atcatcGTCTTGAAGCGTTCTTTCGGCGGGGG‑GAACATTTTTCTTTC‑GGGCGCCCGGTA‑GGCATGACCCTTATTGCG‑ATGGGCGGGGCTTTTTTACTG‑TTACGCGCAATTCAGGGAGTATTTTT‑TAACGTTTCCACGCTACTGGTGTGGGTGCTGGTTGGTTTTCTGGTGT‑TTGGCGCAGGGAAAGTTCGTCTTCATTTTTCTGCTTTTTTGACAGCTGCTTCACGGAATGATAGGCATCTCCGGGCCACg > 2:394221/1‑251 (MQ=255) aGCGGTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAAc < 2:204847/251‑1 (MQ=255) cggcggGTG‑AAAACTTTTTCTCTC‑GGGCGCACGTTA‑GGCCTGACCATTATTGCG‑GTGGGCGTGACTTTTTTTCTG‑TTTCGCGCATTGCAGGGGGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGGTGCTGATTGGTTTGCTG‑TGGATTTGCGGCGGGAAAGTTCGTCTTCCTTATCATTCTTATCTGACCGCTGCTTCACGGAATGATAACCATGCCCGTGCCCCGACGGCTGGCggaaccacccctat > 2:262993/1‑238 (MQ=255) ggcggGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGAtt < 1:366406/251‑1 (MQ=255) ggcggGGG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCAGTCTAGCG‑ATGGGCGTGACTTTCTTAATG‑TTACGCGCATTGCAGGGCGCATTGTT‑CAACGTTCCCACGCTACTGGTGTGGATGCTGGTTGGATTGCTG‑TGTATTCGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGATGCTTCACGTAATGATAGCCATGACCGTGCCACGTTGGCTGGCGAACCCACCATGAtt > 1:349005/1‑251 (MQ=255) cATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGCCTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCCGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAACGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCGCCATGATTCACGGCGTccc > 1:299398/1‑251 (MQ=255) agttttCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCAGTGCAGGGAGTAGTGTG‑CAACGTTCCCACGCTACTGGTGTTGCTGCTGAGTGGGTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCGCGTAATGATAGCCTTTCCCGTGCCACGAGAGCTGGCGAACGCACCATGATTCACGGCGTCCCg < 2:227378/249‑1 (MQ=255) | GATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTG‑AAACATTTTTCTCTC‑GGGCGCACGTTA‑GGCATGACCATTATTGCG‑ATGGGCGTGACTTTTTTACTG‑TTACGCGCATTGCAGGGAGTATTGTT‑CAACGTTCCCACGCTACTGGTGTGGCTGCTGATTGGTTTGCTG‑TGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCG > NC_000913/119152‑119640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |