New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	800643 =	19 (0.300)	3 (0.050)	3/482	3.7	7.7%	intergenic (‑68/‑116)	ybhD/ybhH	putative DNA‑binding transcriptional regulator YbhD/putative isomerase YbhH
	?	NC_000913	= 4460243	53 (0.840)					coding (122/465 nt)	nrdG	anaerobic ribonucleoside‑triphosphate reductase activating protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTTTGTTAACGTAACCTTGCGAGTTGTACGCTCAAATAGTTGAACATGTAAATCTTCCTCCATCTTTTTTATTCTTCGCGTTAATGCAGGTTGCGTAATATTGAGTAATTTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGAT  >  NC_000913/800393‑800890                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        ttttGTTAACGTCCCCTTGCGAGTTGTACGCTAAAATAGTTGGACATGTAAATCTTGATCCATCTTTTTTATTCTTCGAGTTAATGCAAGTTGCGTAATATTGAGTAATTTAGCGGCATTTTTAAACGAACTGGACTCTGCCTGTATGAGAAATGCCTTCATAGTTGTTAATTCATGCTTCATTATTACTCCGGAAAATGGATGCGACGCTTTTGGGAGCCTGGCCGTTAGAAATTTTGACTGCAGTTAGc                                                                                                                                                                                                                                                         >  2:526422/1‑251 (MQ=255)          cGTAACCTTGCGAGTTGTACGCTCAAATAGTTGAACATGTAAATCTTCCTCCATCTTTTTTATTCTTCGCGTTAATGCAGGTTGCGTAATATTGAGTAATTTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTaa                                                                                                                                                                                                                                                 >  2:270183/1‑250 (MQ=255)              aCCTTGCGAGTTGTATGCTCAAATAGTTGAACATGTAAATCTTCCTCCATCTTTTTTATTCTTCGCGTTAATGCAGGTTGCGTAATATTGAGTAATTTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCTGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCATCTTAAATTaa                                                                                                                                                                                                                                            <  1:87749/251‑1 (MQ=255)                                    aTAGTTGAACATGTAAATCTTCCTCCATCTTTTTTATTCTTCGCGTTAATGCAGGTTGCGTAATATTGAGTAATTTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACGTGAGAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGtt                                                                                                                                                                                                                      <  1:346028/251‑1 (MQ=255)                                          gAACATGTAAATCTTCCTCCATCTTTTTTATTCTTCGCGTTACTGCCGGTTGCGTAATATTGACTAATTTAGCGGCCTTGTTAACCGCACTGGACTCTGCCAGTATGACACATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCt                                                                                                                                                                                                                 >  1:376445/1‑250 (MQ=255)                                                 tAAATCTTCCTCCATATTTTTTATTCTTAGCGTTAAAGCAGGTTGCGTAATTCTGATTTATTTAGCGTCATTGTTAAAAGAACTGGCCTCTGCCAGTATGACAAATGCCTTCATACTTGATACTTCATGCTTCATTATTACTCCGGAACATGGACGCGACGATTTTGGTTGGCTGGCCGTAAACAATTTTAAATGCATTTAGCCAACTTACATTAATGATAAAATGTTATTAATCGTTGAGCTAAAGtaat                                                                                                                                                                                                         >  2:63089/1‑248 (MQ=255)                                                             cATCTTTTTTATTCTTCGCGTTAATGCAGGTTGCGTAATATTGAGTAATTTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCttt                                                                                                                                                                                             <  1:407163/251‑1 (MQ=255)                                                                                      gCAGGTTGCGTAATATTGAGTAATTTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGATCTAAAGTCATTAGAGATGCTTTTCCGTTAATGTATCCATATCGCaa                                                                                                                                                                     <  2:376445/250‑1 (MQ=255)                                                                                      gCAGGTTGCGTAATATTGAGTAATGTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAAGTTTAACTGCATTTAGCCAGCTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAAt                                                                                                                                                                    <  1:499617/251‑1 (MQ=255)                                                                                                      tGAGTAATTTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATTGAAGCTACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCAGTAGAGATGCTTTGCCCTTAATGGAACCATATCGCAATAAGGTATGTTTTTaa                                                                                                                                                     <  2:46539/250‑1 (MQ=255)                                                                                                      tGAGTAATTTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTaa                                                                                                                                                     <  2:411435/250‑1 (MQ=255)                                                                                                                   ggCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCCTACTTGATAATTCATGCTTCATTCTTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGACGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCattat                                                                                                                                       >  1:539924/1‑251 (MQ=255)                                                                                                                         gTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGa                                                                                                                                  >  2:470503/1‑250 (MQ=255)                                                                                                                                                               tCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATAGCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATTATGCTAGGTGGAACCTCg                                                                                            <  2:539924/250‑1 (MQ=255)                                                                                                                                                                    cTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTACAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGgc                                                                                      >  2:33771/1‑251 (MQ=255)                                                                                                                                                                        ataattattGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTTGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATGAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTGTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCTAGGTGGAACCTCGAGGTGCGCGt                                                                                  <  2:377335/243‑1 (MQ=255)                                                                                                                                                                         tAATTCATGCTTCATTATCCCTCCGAAAAATGGCAGCGCCGATTTTGGGTGGCTTGCCTTTAAAAATTTTAACTGAATTTAGCCACCTTAAATTATTGAAAAAATGTTATTAATCGTTGAGCTAAAATCCTTAGTGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAACAAATACCCTGCGTGATGATGCGAGGTGGACCCTCGAGGGGCGCGtt                                                                                 >  2:389154/1‑251 (MQ=255)                                                                                                                                                                                      attattACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTACATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGa                                                                     >  1:482660/1‑250 (MQ=255)                                                                                                                                                                                                                                      gaaaaTTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAAtt                    >  2:414389/2‑251 (MQ=255)                                                                                                                                                                                                                                                         aaccAAATTAAAATAAAGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCTCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGAt  <  2:174329/248‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        TTTTGTTAACGTAACCTTGCGAGTTGTACGCTCAAATAGTTGAACATGTAAATCTTCCTCCATCTTTTTTATTCTTCGCGTTAATGCAGGTTGCGTAATATTGAGTAATTTAGCGGCATTGTTAAACGAACTGGACTCTGCCAGTATGACAAATGCCTTCATACTTGATAATTCATGCTTCATTATTACTCCGGAAAATGGAAGCGACGATTTTGGGTGGCTGGCCGTTAAAAATTTTAACTGCATTTAGCCAACTTAAATTAATGAAAAAATGTTATTAATCGTTGAGCTAAAGTCATTAGAGATGCTTTGCCCTTAATGTAACCATATCGCAATAAGTTATGTTTTTAAATTGAGGGCATTATTATGAAAAAAATACCCTGCGTGATGATGCGAGGTGGAACCTCGAGGGGCGCGTTCCTGTTAGCGGAACATTTACCCGAAGATCAAACGCAGCGCGATAAAATATTGATGGCAATTATGGGTTCCGGTAACGAT  >  NC_000913/800393‑800890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.