New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2609308	72 (1.130)	3 (0.050)	3/486	3.7	3.6%	coding (822/1668 nt)	hyfG	hydrogenase 4 catalytic subunit HyfG
	?	NC_000913	3617755 =	90 (1.420)					coding (741/765 nt)	nikD	Ni(2(+)) ABC transporter ATP binding subunit NikD
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTCTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2609059‑2609308 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCGCACGGTGGATTTAACGGAAAACATCAACATCAGGCGGTGCTGAATAACGTTTCCCTGACCCTGAAAAGCGGCGAAACCGTCGCCCTGCTGGGGCGCAG  >  NC_000913/3617755‑3617915                                                                                                                                                                                                                                                                                                                                                                                                                             CACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAACTTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGCGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTCTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCACTTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCC                                                                                                                                                                  >  2:468796/1‑251                                                                                       GCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGCAGAACCCATCTTATCGGACCGCGAGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCAGTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCCTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCGCACGGTGGATTTAACGGAAAACAT                                                                            >  1:228239/1‑251                                                                                                                                                                 ATTTGCGGTTTTGCCCACAGTGTGGCCTAGACCAAGTCGGTTGAAAATGCACAGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCCTACGGTATGTAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCCCACGGTGGATTTAACGTAAAACATCAACATCAGGCGGTGCTGAATAACGTTGCCCTGACCCTGATAAGGGGCGAGACCGTCGTCCTGCTGGGGCGCAG  <  2:228239/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                             CACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGATGGGCTACAACGAAGTGACCTTCTTATCGGACCGCGTGTGTGGGATTTGCGGTTTTGCCCACAGTGTGGCCTATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2609059‑2609308 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTACGGTATGGAGCTGGCATCATGACTTTACTTAACATCTCCGGCCTTTCCCATCACTATGCGCACGGTGGATTTAACGGAAAACATCAACATCAGGCGGTGCTGAATAACGTTTCCCTGACCCTGAAAAGCGGCGAAACCGTCGCCCTGCTGGGGCGCAG  >  NC_000913/3617755‑3617915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.