Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,215	+C	intergenic (‑26/+23)	yjjA ← / ← dnaC	DUF2501 domain‑containing protein YjjA/DNA replication protein DnaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,214	1	.	C	100.0%	166.6 / NA	48	intergenic (‑25/+24)	yjjA/dnaC	DUF2501 domain‑containing protein YjjA/DNA replication protein DnaC
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/22);  total (26/22)

GTTCATGTTATCTGCGCTTAAGGCCTGGTTTCCGCTGCTAAGCAAGTTAGTTAATGACGCGAGCGACCAACCGCCTTCCTGTGTCGTGCTGTTTTGGTTGCCAAGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCG‑CAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGG  >  NC_000913/4599971‑4600452                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               gtgcgtgtggtcaccggtatcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagagagcttcgttcattcctgaTGTGTCGCCAGtat                                                                                                                                                                                                                                          >  1:330265‑M2/238‑251 (MQ=255)            caccggtatcagtttcggttttgtcttctacgtactggaacagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggagcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaacagaatgaacgaataaagggagcgaaagctccccttattattgttagacacgtgtgcttcgttcattcctgaTTCTTCGCCAGTATACCGCCAGaa                                                                                                                                                                                                                                >  2:212766‑M2/227‑250 (MQ=255)               cggtatcagtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACgg                                                                                                                                                                                                                            >  1:72921‑M2/224‑251 (MQ=255)                      agtttcggttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTa                                                                                                                                                                                                                      >  1:164226‑M2/217‑250 (MQ=255)                            ggttttgtcgtcgacgtactggaccagatcttcggcccgctgacgttggtttagggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcatgcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATActct                                                                                                                                                                                                               <  2:87435‑M2/41‑1 (MQ=255)                             gttttgtcttctacgtactggaccagatcttcggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCtt                                                                                                                                                                                                              >  1:401984‑M2/210‑251 (MQ=255)                                     ttttacgtactggcccaaatctgcggcccgctgccgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGt                                                                                                                                                                                                       <  2:3525‑M2/49‑1 (MQ=255)                                       caaagtcctggcccagatctgcggcccgttgacgttggtttgtggcatccctccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaattagaaaatcgtaaccgaatgaacgaataaagggagcgaaagcgccctttattattggtagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTa                                                                                                                                                                                                     <  2:333681‑M2/51‑1 (MQ=255)                                                            cggcccgctgacgttggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATccc                                                                                                                                                                                <  1:339777‑M2/72‑1 (MQ=255)                                                                    ggacgtgggtttatgtcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcatgcctgaTGTGTCGCCAGTATACCGTCAGAAACGGGGATTAATGCTCTTTACCTGTTTCCCGGCTACGGTAGCTATCCCAGTTGAAGa                                                                                                                                                                       <  1:61530‑M2/81‑1 (MQ=255)                                                                          tggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaattagcctgtggctgctactgagaaaatcgtaaccgaatgaacgaataaagggtgcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAg                                                                                                                                                                        >  1:272001‑M2/165‑244 (MQ=255)                                                                           ggtttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCAccc                                                                                                                                                                 >  2:169937‑M2/164‑250 (MQ=255)                                                                              ttatggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCg                                                                                                                                                                                                                                                 >  1:46653‑M2/161‑167 (MQ=255)                                                                              ttagggcatcccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCg                                                                                                                                                                                                                                                 <  2:46653‑M2/7‑1 (MQ=255)                                                                                       cccgccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTa                                                                                                                                                      <  1:323322‑M2/98‑1 (MQ=255)                                                                                          gccgatcatcggcgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAccc                                                                                                                                                   >  2:421214‑M2/149‑249 (MQ=255)                                                                                                      cgcactgttgccaagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTc                                                                                                                                     <  1:195778‑M2/115‑1 (MQ=255)                                                                                                      cgcactgttgccaagcgccaccttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaaagaacgaataaagggagcgaaagctccctttattattgttagcaaagtgagcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACATGTTACCCCGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCACATCCGGTc                                                                                                                                     >  2:557320‑M2/137‑251 (MQ=255)                                                                                                         actgttgccaagcgcaagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtcaccgaatgaacgaatacagggagcgaaagctccctttattattgttagcaaagggtgcttcgctcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTCCCTGTTCACCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTCCCCAGGCGCATACGGTCCAt                                                                                                                                  >  2:484412‑M2/134‑251 (MQ=255)                                                                                                               gccaagggccagcttcttcttaatcagcctgtggctgttaatgaggaaatcgtaaccaaataaacgaattaagggagcgaaagctccctttattattgttagcaaagtgtgcttagttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCCGGAGCATACGGTCCATCAcgcg                                                                                                                            >  2:256625‑M2/128‑251 (MQ=255)                                                                                                                 caagcgccagcttcttcttaatcagcctgtggctgttactgagaaaatcgtacccgaatgaacgaataaagggagcgaaagctccctttattattgttcgcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAcg                                                                                                                              >  1:61363‑M2/126‑247 (MQ=255)                                                                                                                 caagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAcg                                                                                                                              <  2:61363‑M2/122‑1 (MQ=255)                                                                                                                  aagcgccagcttcttcttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTc                                                                                                                         >  1:397653‑M2/125‑251 (MQ=255)                                                                                                                                 cttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGt                                                                                                                                                        <  1:195356‑M2/96‑1 (MQ=255)                                                                                                                                 cttaatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGt                                                                                                                                                        >  2:195356‑M2/110‑205 (MQ=255)                                                                                                                                    aatcagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtttgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTTTTACCCATGCGCATACGGGCCATCACGCGTTCGGCCAGCGACGTGGTCAt                                                                                                       <  2:207258‑M2/145‑1 (MQ=255)                                                                                                                                    aaacagacggtggcgcttaatgagaaaatcgtaacagaatgaacgaataaagggagtgaaagcttcctttattattgttagcaaagtgttcttcgttcatgtctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTGGAAGATCACCCACAAACTGTTACCCAGGCGAATACGGTCGATCACGCGTTCGCCCCGCAACTTGGGCAt                                                                                                       <  2:186994‑M2/145‑1 (MQ=255)                                                                                                                                       cagcctgtggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAtctc                                                                                                    >  1:387540‑M2/104‑251 (MQ=255)                                                                                                                                               ggctgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCatat                                                                                            <  2:320684‑M2/156‑1 (MQ=255)                                                                                                                                                  tgttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaacgctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTAc                                                                                         >  1:364437‑M2/93‑251 (MQ=255)                                                                                                                                                   gttaatgagaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTAc                                                                                         <  1:169830‑M2/159‑1 (MQ=255)                                                                                                                                                           gaaaatcgtaaccgaatgaacgaataaagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTc                                                                                 >  1:426404‑M2/84‑250 (MQ=255)                                                                                                                                                                                     aagggagcgaaagctccctttattcttgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaaga                                                       >  1:204893‑M2/58‑250 (MQ=255)                                                                                                                                                                                     aagggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaaga                                                       >  1:18151‑M2/58‑250 (MQ=255)                                                                                                                                                                                        ggagcgaaagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGaagaag                                                     >  1:139295‑M2/55‑249 (MQ=255)                                                                                                                                                                                               aagctccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATGCTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGAt                                            >  1:118938‑M2/48‑251 (MQ=255)                                                                                                                                                                                                    ccctttattattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATcgac                                        <  1:117672‑M2/208‑1 (MQ=255)                                                                                                                                                                                                          attattgttagcaaagtgggctgcgttcattccttaTGTGTCGCCAGTAGACCGCCAGAAACGGGGATTAATACTCTTTACCTTTTACCCGGCTACGGAAGCTATCCCAGTTGAAGATCACCCGCAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCATCTTGGTCATCTCTTCCATATTACTGTTGGGCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGACGATCGACGATCTg                                  <  2:204893‑M2/214‑1 (MQ=255)                                                                                                                                                                                                            tattgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGtt                               >  1:474243‑M2/35‑251 (MQ=255)                                                                                                                                                                                                             attgttagcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGtt                               <  1:365207‑M2/217‑1 (MQ=255)                                                                                                                                                                                                                   agcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTgatgat                         <  1:421214‑M2/223‑1 (MQ=255)                                                                                                                                                                                                                   agcaaagtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATc                        <  2:139295‑M2/224‑1 (MQ=255)                                                                                                                                                                                                                        agtgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACttt                   <  2:290309‑M2/229‑1 (MQ=255)                                                                                                                                                                                                                          tgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACt                                                                                        <  2:220994‑M2/160‑1 (MQ=255)                                                                                                                                                                                                                          tgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACt                                                                                        >  1:220994‑M2/21‑180 (MQ=255)                                                                                                                                                                                                                          tgtgcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACttttt                 >  2:295550‑M2/21‑251 (MQ=255)                                                                                                                                                                                                                             gcttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGt              >  2:188805‑M2/18‑251 (MQ=255)                                                                                                                                                                                                                              cttcgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGt              >  2:491758‑M2/17‑250 (MQ=255)                                                                                                                                                                                                                                 cgttcattcctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACGCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTAttt          <  2:401984‑M2/238‑1 (MQ=255)                                                                                                                                                                                                                                         cctgaTGTGTCGCCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCgg  <  1:403667‑M2/246‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               GTTCATGTTATCTGCGCTTAAGGCCTGGTTTCCGCTGCTAAGCAAGTTAGTTAATGACGCGAGCGACCAACCGCCTTCCTGTGTCGTGCTGTTTTGGTTGCCAAGTTCGCTGGCGGCGCTGGAGAGCGCATCTTTCCAGGACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCG‑CAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGG  >  NC_000913/4599971‑4600452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑