Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,362,045	T→C	intergenic (‑111/+506)	cadC ← / ← pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,362,045	0	T	C	100.0%	188.1 / NA	52	intergenic (‑111/+506)	cadC/pheU	DNA‑binding transcriptional activator CadC/tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/29);  total (23/29)

GTGTTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTTTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCG  >  NC_000913/4361806‑4362278                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          gtgtTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGACCAAAGGTAAGTTGACGCCCATTGCGGATAATTTGGCTTATGGACGGCGTAACAAGCCATTCGCCAACGCGCACTACAGGTTGTTGCATAATAGAAACTCATTCGACCAGAGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCAt                                                                                                                                                                                                                                >  1:255050/1‑251 (MQ=255)          aaaGAAAACCAGAAGATCTATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGAAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAAAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTAGTTTTTCCATCTATAGATGGGTTTATTTATATAGTAGTGGGAAATGCAATACGTTATTCTGACCAGCCATAAActtctt                                                                                                                                                                                                                       <  2:629115/251‑1 (MQ=255)          aaaGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAActtctt                                                                                                                                                                                                                       >  1:467666/1‑251 (MQ=255)              aaaaCCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACAGATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGAtt                                                                                                                                                                                                                   <  1:332328/251‑1 (MQ=255)                    agaagaTCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCCTTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACAt                                                                                                                                                                                                               >  2:410960/1‑249 (MQ=255)                    agaagaTCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAg                                                                                                                                                                                                             <  1:557680/251‑1 (MQ=255)                               tAATCCCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGaaa                                                                                                                                                                                                   <  2:491332/250‑1 (MQ=255)                                aaTCTCGGCTCACGGGTAACTTGACGCCAATTGAGGCTAAATTGGTTTATGGACGGAGTAACAAGCAAATCGCCAACGCGAACTCCAGGTTGTTGCATAATCGAAACTAATTCGAAACGGGAATGATGCAATTATAATTCCCAAAACCTATTTTTACCATCTATAGATGGGATTATTTACATCTTATTGGTGAATGCAAGACGTTCTTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGaacgg                                                                                                                                                                                                 >  2:61527/1‑248 (MQ=255)                                 aTCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTACTTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAgg                                                                                                                                                                                                 >  1:288652/1‑250 (MQ=255)                                     cGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGAtt                                                                                                                                                                                              <  1:467577/249‑1 (MQ=255)                                       gCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTAc                                                                                                                                                                                          >  2:183182/1‑251 (MQ=255)                                                tAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAAcc                                                                                                                                                                                  >  1:326924/1‑250 (MQ=255)                                                            cATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTc                                                                                                                                                                     <  2:127490/251‑1 (MQ=255)                                                                 cGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTa                                                                                                                                                                 >  1:120527/1‑250 (MQ=255)                                                                 cGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTAc                                                                                                                                                                >  2:383967/1‑251 (MQ=255)                                                                  ggCTAATTTGGTTTATGGACGGAGTGACAAGCCATTCGCCAACGCGAACTACCGGTTGTTGCATAATCGACACTCATTCAACAAGGGAATGATGCCATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGCGAACCAGAAGTAATATTACTTAcc                                                                                                                                                               >  2:401374/1‑251 (MQ=255)                                                                      aaTTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCTAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAATGATTTTACTGAGAACCAGAAGTAATATTCCTTACCAt                                                                                                                                                             <  1:625170/249‑1 (MQ=255)                                                                       aTTTGGTTTATGGATGGAGAAACAAGCCATTCGCCAACGCGAACTGCAGGATGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCCCAACCTATTTTTACCATATATAGATGGGTTTATTTACATATTATTGGTGCATGCAAGCCGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCa                                                                                                                                                           >  2:106743/1‑250 (MQ=255)                                                                                  ggacgagtaaaCAAGCCATTCCCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGGGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACTAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTGACCATCAAAATGcatcat                                                                                                                                                <  2:298948/241‑1 (MQ=255)                                                                                  ggACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCatcatc                                                                                                                                               <  2:329317/251‑1 (MQ=255)                                                                                   gACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCt                                                                                                                                              >  1:5050/1‑251 (MQ=255)                                                                                    aCGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCtt                                                                                                                                             <  2:129477/251‑1 (MQ=255)                                                                                    aCGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCtt                                                                                                                                             <  2:89430/251‑1 (MQ=255)                                                                                     aggAGTAACAAGCCATGCGCCAAAGCGAACTACAGGTTTTTGCATAATAGAAACGCATTCGAAAAGGGAATGATGAAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATAGTATTGGTGAATGCAAGACGTTGTTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAAGATTCCTTACCGTCAAAATTCATCATCttt                                                                                                                                            <  2:313522/250‑1 (MQ=255)                                                                                         gTAACAAGCCATTCGCCAACGCGAACTACAGGCTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGcc                                                                                                                                         >  1:19703/1‑250 (MQ=255)                                                                                           aaCAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAAGAGAAACTCATTCGAAAAGGGAAGGATGCAATGAAAATTGCCACAACCTATTTTTGCCAGCTATAGATGGGTTTATTTACATATGATTGGTGAAGGCAGGACGTTATTCTGACCAGCCAGAAACTTCTTGATTACATAGTATGACGAAAGTATTTTACTGAGAACCAGAAGTAATAGTCCTTACCATCAAATTTCATCAGCTTTGCCaaa                                                                                                                                      <  2:120527/251‑1 (MQ=255)                                                                                             cAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGa                                                                                                                                    <  1:71593/251‑1 (MQ=255)                                                                                              aaGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGa                                                                                                                                    >  2:290954/1‑250 (MQ=255)                                                                                                  cATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAAt                                                                                                                               >  1:572215/1‑251 (MQ=255)                                                                                                          aaCGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAACATGTTCAGa                                                                                                                        >  2:412042/1‑250 (MQ=255)                                                                                                          aaCGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGaa                                                                                                                       <  2:136526/251‑1 (MQ=255)                                                                                                                       ggTTGTTGCATAATAGCAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATgg                                                                                                           >  1:571901/1‑250 (MQ=255)                                                                                                                            ttgCATAATAAAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAAt                                                                                                      <  2:388431/250‑1 (MQ=255)                                                                                                                                  aataGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCgg                                                                                               >  2:379859/1‑251 (MQ=255)                                                                                                                                     aGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGAAGc                                                                                            >  2:88690/1‑251 (MQ=255)                                                                                                                                                     aGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATg                                                                            >  1:624972/1‑251 (MQ=255)                                                                                                                                                          atgatgCAATGATAATTGCCACAACCTATTTTTACCACCTATAGATGGGTTTATTTACATATTATTGGTGAAGGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGAGTTCGCTGAGAACCAGAAGTAAGATTCCTTACCATCAAAAGTCATCGTCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTg                                                                       <  1:302076/251‑1 (MQ=255)                                                                                                                                                                    gATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCttt                                                              <  2:209609/250‑1 (MQ=255)                                                                                                                                                                    gATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAAGTGTCCGGAGCCCTTACTATTTTAATGGATTGTTAGTCTTTg                                                             <  1:235321/251‑1 (MQ=255)                                                                                                                                                                     aaaaTTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGc                                                            <  1:594069/249‑1 (MQ=255)                                                                                                                                                                           gCCACAACCTATTTTTACCATCTATAGAGGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGGTTGTTAGTCTTTGCATGAGc                                                      <  2:6681/251‑1 (MQ=255)                                                                                                                                                                                  ccTATTTTTACCCTCTAAAGACGGGATTATTTACATATTAACGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTCCCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGa                                                >  2:35883/1‑250 (MQ=255)                                                                                                                                                                                          tACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATAc                                        <  1:383967/250‑1 (MQ=255)                                                                                                                                                                                              aTCTATAGATGGGTTTCTTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCCTAAACTTCTTGATTACATAGTATTACGAAAGGATTTTCCTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTtc                                   >  2:260538/1‑251 (MQ=255)                                                                                                                                                                                                  ataGAGGGTTTTATTTACATATTATTGGAGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGAGACATTtctc                                 <  2:619646/249‑1 (MQ=255)                                                                                                                                                                                                    tgATGGTTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTCCCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTTTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTGTGCATGAGCAAGCGAACTGATACATTTCTCttt                              <  2:480876/249‑1 (MQ=255)                                                                                                                                                                                                     gATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATg                                                                                                            >  1:484654/1‑171 (MQ=255)                                                                                                                                                                                                     gATGGGTTTATTTACAAATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATg                                                                                                            <  2:484654/171‑1 (MQ=255)                                                                                                                                                                                                                ttaCATATTATTGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAg                  <  2:375106/250‑1 (MQ=255)                                                                                                                                                                                                                         attGGTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcat        <  1:290954/251‑1 (MQ=255)                                                                                                                                                                                                                            ggTGAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcatca      <  2:195640/250‑1 (MQ=255)                                                                                                                                                                                                                               gAATGCAAGACGTTATTCTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCg  <  1:183182/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                          GTGTTGAGCAAAGAAAACCAGAAGATCGATTAATCTCGGCTCAAGGGTAAGTTGACGCCCATTGCGGCTAATTTGGTTTATGGACGGAGTAACAAGCCATTCGCCAACGCGAACTACAGGTTGTTGCATAATAGAAACTCATTCGAAAAGGGAATGATGCAATGATAATTGCCACAACCTATTTTTACCATCTATAGATGGGTTTATTTACATATTATTGGTGAATGCAAGACGTTATTTTTACCAGCCATAAACTTCTTGATTACATAGTATTACGAAAGGATTTTACTGAGAACCAGAAGTAATATTCCTTACCATCAAAATTCATCATCTTTGCCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCG  >  NC_000913/4361806‑4362278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑