Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,174,041	A→T	E320D (GAA→GAT)	birA →	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,174,041	0	A	T	100.0%	223.3 / NA	63	E320D (GAA→GAT)	birA	DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (34/29);  total (34/29)

GGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCGGTAAAAGCCCCTTCGC  >  NC_000913/4173795‑4174284                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ggcggcCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGataaa                                                                                                                                                                                                                                                  <  1:480098/250‑1 (MQ=255)    ggcCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATa                                                                                                                                                                                                                                                >  1:612512/1‑249 (MQ=255)      ccATGCTAATACGTGAATTACGTGCTGAGTTCGAACTCTTACAACAAGAAGGATTGGCACCTTATCTGTCGAGCTGGGAACAGCTGGATAATTTTATTACTCGCACAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGTGGAATAGACAAACAGGGGGCTTTCTTACTTGAGCAGGATGAAATAATCAAACACTGGATGGTCGGCGAAATATCCCTGCGTAGTGCCGATAAATAAGa                                                                                                                                                                                                                                             >  1:677901/1‑250 (MQ=255)                  gTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGACCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTc                                                                                                                                                                                                                                <  2:464016/251‑1 (MQ=255)                    gAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGc                                                                                                                                                                                                                              <  2:75828/251‑1 (MQ=255)                        tACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTcc                                                                                                                                                                                                                           <  1:268834/250‑1 (MQ=255)                         aCGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTcccc                                                                                                                                                                                                                         <  1:149651/251‑1 (MQ=255)                         aCGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTcccc                                                                                                                                                                                                                         <  1:61189/251‑1 (MQ=255)                            tgctgcGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGAGCTGGGAAAAGCTGGATACTTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGGTGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGc                                                                                                                                                                                                                      >  2:619676/1‑251 (MQ=255)                                  gTTGGAACTCTTCGAACACGAAGGATTGGCCCCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAACCTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAatt                                                                                                                                                                                                                 >  2:622960/1‑250 (MQ=255)                                   ttGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAattat                                                                                                                                                                                                               >  1:367189/1‑251 (MQ=255)                                      gAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAAGTATTTg                                                                                                                                                                                                            <  1:509193/251‑1 (MQ=255)                                             tCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTAGTTGCGTAGt                                                                                                                                                                                                      <  1:208237/250‑1 (MQ=255)                                                  cAAGAAGGATTGGCACCTGATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGAGAAAGAAATATTTTGCATTTCACGCTGAATAGACAAACAGGCGGATTGATTACTTGAGCCGGATTGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGTTAAATAATAAAGGTGATTATTCTCTCCCCTACAAATTAGTTGCGTTGTCTgtcc                                                                                                                                                                                                <  2:440243/251‑4 (MQ=255)                                                      aagGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATCCTTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCt                                                                                                                                                                                                                        >  1:321702/1‑223 (MQ=255)                                                      aagGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCtctt                                                                                                                                                                                            >  1:168455/1‑251 (MQ=255)                                                      aagGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACGTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGGAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCt                                                                                                                                                                                                                        <  2:321702/223‑1 (MQ=255)                                                            tGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTAcc                                                                                                                                                                                       <  1:292522/250‑1 (MQ=255)                                                                    tATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGAtt                                                                                                                                                                               >  1:472126/1‑250 (MQ=255)                                                                    tATCTGGCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGAtt                                                                                                                                                                               >  1:145906/1‑250 (MQ=255)                                                                        tGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCAc                                                                                                                                                                          >  1:363954/1‑251 (MQ=255)                                                                             cgcTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTGATCATTGGTGATAAAGAAATATTTGGCAGTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGATCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGCAAGGGGAGTATTCGCTCGGCTGCAAATTATTTGCGTAGACTGACCTCGACTACCGCATGATTAGcccgtttc                                                                                                                                                                     <  2:125543/251‑8 (MQ=255)                                                                                  ggAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAg                                                                                                                                                                <  1:643002/251‑1 (MQ=255)                                                                                   gAAAAGCTGGATAATTTTATTAATCGCCCAGTGAACCTTCTAATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACCAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATACAACCATGGATGAGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCACTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAgg                                                                                                                                                               >  2:409288/1‑251 (MQ=255)                                                                                           ggATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAAc                                                                                                                                                        <  2:94138/250‑1 (MQ=255)                                                                                           ggATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACt                                                                                                                                                       >  1:165420/1‑251 (MQ=255)                                                                                                   ttattaATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGgcgcgc                                                                                                                                                <  1:486536/250‑1 (MQ=255)                                                                                                                   gAAACTTCTCATTGGTGATAAAGCAATATTTGGCATTTCACGCGGACTAGACAAACAGGGGGCTTTATTACTTGAGAAGGATGGAATAATACAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGCTCAGCACTTTTCGTCAGGATTAAACTGTCGCGCTCACGCGTAGGTAGAat                                                                                                                               >  2:574146/1‑251 (MQ=255)                                                                                                                   gAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAat                                                                                                                               >  1:87575/1‑251 (MQ=255)                                                                                                                       cTTATCATTGGTGATCAAGAACTATTTGGAATTTCACGCGGAATACACAAACCGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTCGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGAATAAACTGGCGCGCTCACGAGTAGGTAGAATAttt                                                                                                                           >  2:660709/1‑251 (MQ=255)                                                                                                                                  tGATAAAGAAATATTTGGCATTTCACGCGGAATCGACACACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGtt                                                                                                                >  2:112672/1‑251 (MQ=255)                                                                                                                                   gATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTc                                                                                                               <  1:495260/251‑1 (MQ=255)                                                                                                                                   gATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATGCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGGCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTc                                                                                                               <  1:622960/251‑1 (MQ=255)                                                                                                                                      aaaGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACATGAGCATGATGGAAGAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGAGGAGTATTCGCTCCCCTGCAAATTATTTGCGGAGTCTGACCTCTTCTACCGCATGATTAGCACGTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAAGATTTTGCTTTAAGTTCAGc                                                                                                            <  2:367189/251‑1 (MQ=255)                                                                                                                                           aaTATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATCTTTTGCTTTAAGTTCAGCCAGtt                                                                                                       >  2:507589/1‑251 (MQ=255)                                                                                                                                              atTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGTAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCGACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCttt                                                                                                                     <  1:636688/234‑1 (MQ=255)                                                                                                                                              atTTGGAAGTTCCCGCGGAATAGACAAATAGGGGGCTTTATTCCTTGAGCTGGCTGGATTAATAAACCTCTGGACGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCACATTATTTGCGTAGTCTGACCTCTTCTACCGCATGCTTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGCATATTTTGCttt                                                                                                                     >  2:636688/1‑234 (MQ=255)                                                                                                                                                ttGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGAtc                                                                                                   <  2:194640/250‑1 (MQ=255)                                                                                                                                                ttGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGAATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGAAGATAAATAAGAAAGGGGAGTATTCTCTCCCCTGCAAATTATTTGCTTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTTCTTTAAGTTCGGCCAGTTGAtc                                                                                                   <  1:571310/250‑1 (MQ=255)                                                                                                                                                  ggCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTGTGCTGTAAGTTCAGCCAGTTGAtctc                                                                                                 <  1:189820/250‑1 (MQ=255)                                                                                                                                                    cATTTCACGCGGAATAGACAAACAGGGTGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGTTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCttt                                                                                              <  2:612512/251‑1 (MQ=255)                                                                                                                                                     aTTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTc                                                                                             >  1:161549/1‑251 (MQ=255)                                                                                                                                                     aTTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTc                                                                                             >  1:344852/1‑251 (MQ=255)                                                                                                                                                          aCGCGGCATAGCCATACAGGGGGATTTATTATTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATCTCCCTGCGTAGTGCAGATACATAAGACAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATCTTTTGCTTTACGTTCAGCCAGTTGATCTCTTTCcaca                                                                                         >  2:259739/1‑250 (MQ=255)                                                                                                                                                               gAATAGCCAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATACCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAATTTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTCGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCCCCATGTc                                                                                    >  2:68097/1‑250 (MQ=255)                                                                                                                                                                   aGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGc                                                                               >  1:517352/1‑251 (MQ=255)                                                                                                                                                                    gACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGCGTATTCGCTCCCCTGCAAATTATTTGTGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGc                                                                               >  1:434331/1‑250 (MQ=255)                                                                                                                                                                       aaaCAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGTTGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTc                                                                           >  2:58725/1‑251 (MQ=255)                                                                                                                                                                                  tttATTACTTGAGCAGGATGGCATAATAAAACCCTGGATGGGCGGTGCAATATCCCTGCGTAGTGCAGATAACTAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGcttc                                                                >  2:412389/1‑251 (MQ=255)                                                                                                                                                                                        aCTTGAGCAGGATGGAATAATAAAACCCTGTATGGGCGGTGAAATATCCCGTCGTAGTGCAGATAAATAAGAAAGGGGAGTAGTCGCTCCCCTGCAAATTAATTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGcttcttcgt                                                           <  2:15246/250‑3 (MQ=255)                                                                                                                                                                                         cTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCttt                                                          >  1:301087/1‑250 (MQ=255)                                                                                                                                                                                           tGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAg                                                        <  1:479739/250‑1 (MQ=255)                                                                                                                                                                                                             aaaaCCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                                     >  2:246237/1‑251 (MQ=255)                                                                                                                                                                                                              aaaCCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                                     >  1:524899/1‑250 (MQ=255)                                                                                                                                                                                                                aCCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGaaa                                  <  1:528627/251‑1 (MQ=255)                                                                                                                                                                                                                       aTGGGCGGTGAAATATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGaa                           <  1:616099/251‑1 (MQ=255)                                                                                                                                                                                                                                gAAATATCCCTGCGTAGTGCAGATAAATACGAAACGGGAGTATTCGCTCCCCTGAAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGAAGTCTTAATCGCTTCTTCTTTAGTTAATTCCGTGTAGTTATGAAAATAGGAATCCGGGTCg                  >  2:599212/1‑251 (MQ=255)                                                                                                                                                                                                                                 aaaTATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCgg                 >  1:223906/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaTATCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCATCCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGCTAAGCACTTTTCGTCAGGATTCAACTGGCGCGCTCACGAGTAGGCAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTCATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCGGt                >  1:265711/1‑251 (MQ=255)                                                                                                                                                                                                                                   atatCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                                     >  1:452610/1‑229 (MQ=255)                                                                                                                                                                                                                                   atatCCCTGCGTAGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCGTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCAGGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATg                                     <  2:452610/229‑1 (MQ=255)                                                                                                                                                                                                                                               aGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTGGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGGTATGAAAATAGGAATCCGGGTCGGTAAAAGCCCCTTc    <  1:599786/250‑1 (MQ=255)                                                                                                                                                                                                                                               aGTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGAAAATAATTTGCGTAGTCTGACCTCTTCAACCGCCTACTTAGCACTTTTCGTCCGGACTAAACTGCCGCGCTAAAGAGTAGGTAGACTCTTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTCGTTAATTTCGCGTAGTTATGAACATAGGAATCCGGGTCGGTAAAAGCCCCTTcg   >  1:665314/1‑251 (MQ=255)                                                                                                                                                                                                                                                gTGCAGATAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCGGTAAAAGCCCCTTcgc  <  2:165420/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATAATTTTATTAATCGCCCAGTGAAACTTATCATTGGTGATAAAGAAATATTTGGCATTTCACGCGGAATAGACAAACAGGGGGCTTTATTACTTGAGCAGGATGGAATAATAAAACCCTGGATGGGCGGTGAAATATCCCTGCGTAGTGCAGAAAAATAAGAAAGGGGAGTATTCGCTCCCCTGCAAATTATTTGCGTAGTCTGACCTCTTCTACCGCATGATTAGCACTTTTCGTCAGGATTAAACTGGCGCGCTCACGAGTAGGTAGAATATTTTGCTTTAAGTTCAGCCAGTTGATCTCTTTCCACAATGTCATGGCAGTCTTAATCGCTTCTTCTTTAGTTAATTTCGCGTAGTTATGAAAATAGGAATCCGGGTCGGTAAAAGCCCCTTCGC  >  NC_000913/4173795‑4174284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑