Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,582	C→A	C44F (TGC→TTC)	ysaB ←	putative lipoprotein YsaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,582	0	C	A	100.0%	284.9 / NA	78	C44F (TGC→TTC)	ysaB	putative lipoprotein YsaB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/36);  total (42/36)

AAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATTTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCC  >  NC_000913/3725348‑3725827                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      aaaGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctc                                                                                                                                                                                                                                       <  2:448915‑M1/251‑10 (MQ=255)  aaggaaggGATCAGTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcg                                                                                                                                                                                                                                      <  1:119052‑M1/251‑11 (MQ=255)    ggaaggGATCCGTATACCCGCTGGATGGAAGATCTACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACCGACAAACTGCTCTTTACGGAAGGTATAAACGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGAGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcgg                                                                                                                                                                                                                                     >  2:540503‑M1/1‑239 (MQ=255)             cAGTATGCCCGCTGGATGGAAGATATAAAGTAAACAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCAATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgttt                                                                                                                                                                                                                           >  2:249519‑M1/1‑230 (MQ=255)               gTATACCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttat                                                                                                                                                                                                                         >  2:249658‑M1/1‑228 (MQ=255)                 ataCCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatc                                                                                                                                                                                                                        >  1:196767‑M1/1‑226 (MQ=255)                   aCCCGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagt                                                                                                                                                                                                                     >  1:4757‑M1/1‑224 (MQ=255)                      cGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCACAAGAACCGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgtt                                                                                                                                                                                                                  >  1:670456‑M1/1‑221 (MQ=255)                      cGCTGGATGGAAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgtt                                                                                                                                                                                                                  >  2:484921‑M1/1‑221 (MQ=255)                      cGCTGGATGGAAGATATACAGGACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTGGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggccgcggattcacgtttatcagtgtt                                                                                                                                                                                                                  <  2:92592‑M1/251‑31 (MQ=255)                       gcgggatggaagaTATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATTGAAAGATGCAAAAAATGGACATCCGCGGCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCTCCTGATCTTTGAATAACGCatcggccgcggattcacgtttatcagtgttg                                                                                                                                                                                                                 <  2:237635‑M1/248‑32 (MQ=255)                       gCTGGATGGAAGATATACAGTACAAAACGGGAACAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGCCAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttg                                                                                                                                                                                                                 >  2:346635‑M1/1‑220 (MQ=255)                          ggatggaAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgttt                                                                                                                                                                                                              <  2:278269‑M1/251‑35 (MQ=255)                            atggaAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgttttt                                                                                                                                                                                                            >  1:300235‑M1/1‑215 (MQ=255)                             tggaAGATATACAGTACAACACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGCATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttg                                                                                                                                                                                                           >  1:149612‑M1/1‑214 (MQ=255)                              ggaAGATATACAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgg                                                                                                                                                                                                          >  1:526737‑M1/1‑213 (MQ=255)                               gaAGATATAAAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttggg                                                                                                                                                                                                         >  1:547800‑M1/1‑212 (MQ=255)                                        cAGTACAAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGAGTCACAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGAGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggccgcc                                                                                                                                                                                                >  2:216171‑M1/1‑203 (MQ=255)                                                 acggggcaaagcagggcTTAACGCATGGAACGATGCACAAACTGGACATCCGCGTCAAAAGAACAGCCAAACTGCTCTTTACGGAAGGCATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGACGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCAGCCTGATCCTTGACTACCGCctcggcctcggattcacgtttatcagtgttgtttttgggctggaagccagaaggg                                                                                                                                                                                         >  2:206797‑M1/8‑194 (MQ=255)                                                  cGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCGGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACTTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagt                                                                                                                                                                                      <  2:478814‑M1/251‑59 (MQ=255)                                                   gggAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAACAACGCCCCTCAGCGACAAAAGACCAGACAACCTGCTCTTTCCGGAAGGTATAACCGCGAATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGCCGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagcagggagtc                                                                                                                                                                                     >  1:637355‑M1/1‑192 (MQ=255)                                                   gggAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCAGCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttggttttgggctggcagccagaagggagtc                                                                                                                                                                                     <  2:87115‑M1/251‑60 (MQ=255)                                                       aaaaGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggc                                                                                                                                                                                 <  1:242244‑M1/251‑64 (MQ=255)                                                              gggCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatatt                                                                                                                                                                          <  1:562175‑M1/251‑71 (MQ=255)                                                              gggCTTAACGCATGGAAAGATGCAAAAAATGGCCAGCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatatt                                                                                                                                                                          <  1:469790‑M1/251‑71 (MQ=255)                                                                       caatgGAAAGATGCAAAAAATGGCCAGCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggccgcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaata                                                                                                                                                                 <  2:261316‑M1/249‑80 (MQ=255)                                                                            gAAAGATGCAAAAAATGGCCATCAGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccg                                                                                                                                                            >  1:226424‑M1/1‑167 (MQ=255)                                                                              aaGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccggg                                                                                                                                                          <  1:249658‑M1/251‑87 (MQ=255)                                                                              aaGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccggg                                                                                                                                                          >  2:301002‑M1/1‑165 (MQ=255)                                                                                  tGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcg                                                                                                                                                      >  1:549903‑M1/1‑161 (MQ=255)                                                                                   gCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCATGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgc                                                                                                                                                     >  2:276927‑M1/1‑160 (MQ=255)                                                                                     aaaaaaTGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgattttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcg                                                                                                                                                    >  1:619501‑M1/1‑158 (MQ=255)                                                                                      aaaaaTGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgtgttcgcgat                                                                                                                                                  <  2:210080‑M1/251‑95 (MQ=255)                                                                                       aaaaTGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgatt                                                                                                                                                 <  1:166685‑M1/251‑96 (MQ=255)                                                                                          aTGGCCATCCGCGTCAAAAGAACAGACAAACTGCGCTGTACGGAAGGTATAACCGCGCATTGCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggatgcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggcggatattttgacaataatccggttgcgcgattctc                                                                                                                                              <  2:315467‑M1/251‑99 (MQ=255)                                                                                                          aaaGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaa                                                                                                                                 >  1:410009‑M1/1‑137 (MQ=255)                                                                                                            aGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaat                                                                                                                            >  1:259781‑M1/1‑135 (MQ=255)                                                                                                            aGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTGCATAGCTTCCCTGGAACTGTTCGAAGGCGGTTACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcgtattcgcgtttatcagtgttgtttttgggctggcagctagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaat                                                                                                                            <  1:660622‑M1/251‑117 (MQ=255)                                                                                                                   aCAAACTGCTCTTTACGGAAGGGATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataattttt                                                                                                                     >  1:601888‑M1/1‑128 (MQ=255)                                                                                                                               ttACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatg                                                                                                           >  1:81063‑M1/1‑116 (MQ=255)                                                                                                                               ttACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatg                                                                                                           <  2:81063‑M1/249‑134 (MQ=255)                                                                                                                                  cGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttg                                                                                                      <  1:164110‑M1/251‑139 (MQ=255)                                                                                                                                     atggtagacccgcgcattccAGAGCTGCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcccgtgtatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataaccccaaagaataatgtttagaggtgatgagttgctt                                                                                                   <  2:574771‑M1/242‑142 (MQ=255)                                                                                                                                         tataACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGCTTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagcagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagtt                                                                                               >  1:396425‑M1/1‑106 (MQ=255)                                                                                                                                            aaCCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCAGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttac                                                                                             >  2:588538‑M1/1‑103 (MQ=255)                                                                                                                                             aCCGCGCATTTCATAGCTTCCCTGGAAATGTTCGAAGGCGGAGACGTCGATTTTATGCTCGCCGGGGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacat                                                                                           >  2:9320‑M1/1‑102 (MQ=255)                                                                                                                                                 cgcATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacg                                                                                       <  1:588538‑M1/251‑154 (MQ=255)                                                                                                                                                       tCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgt                                                                                  <  2:300235‑M1/250‑159 (MQ=255)                                                                                                                                                        cATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCTGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtat                                                                                <  1:16424‑M1/251‑161 (MQ=255)                                                                                                                                                        cATAGCTTCCCTGGAACTGTACGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttttttgtgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttgcataacgattgtat                                                                                <  2:257241‑M1/251‑161 (MQ=255)                                                                                                                                                           aGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgac                                                                             >  2:68083‑M1/1‑88 (MQ=255)                                                                                                                                                                       aCTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggcgtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataac                                                                  >  2:65834‑M1/1‑76 (MQ=255)                                                                                                                                                                          gTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgc                                                              >  1:588681‑M1/1‑73 (MQ=255)                                                                                                                                                                           ttCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCAGCCTGATCCTTGAATAACGCatcggcctcggcttcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccggcttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgct                                                             >  1:337017‑M1/1‑72 (MQ=255)                                                                                                                                                                              gAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgta                                                          <  2:138418‑M1/251‑183 (MQ=255)                                                                                                                                                                                 ggCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgt                                                           >  1:184859‑M1/1‑66 (MQ=255)                                                                                                                                                                                     gTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcaca                                                   <  1:480369‑M1/251‑190 (MQ=255)                                                                                                                                                                                     gTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcac                                                    >  1:576147‑M1/1‑62 (MQ=255)                                                                                                                                                                                          gTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggttatgagttgcttagttacataacgagtgtatgacgaaggcataacatgctgtagatcacatcagg                                              <  1:249519‑M1/251‑195 (MQ=255)                                                                                                                                                                                           tCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacat                                                                <  2:8489‑M1/232‑177 (MQ=255)                                                                                                                                                                                           tCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacat                                                                >  1:8489‑M1/1‑56 (MQ=255)                                                                                                                                                                                             gATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtga                                           <  2:547800‑M1/251‑198 (MQ=255)                                                                                                                                                                                                ttttgcatccccggttttataaCGTTGGGCCGCCTGATACTGTAATAACGCatcggcgtcggattcacgtttatgagtgttgtgtttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgatactcgccataacaccaaagaataatttttagaggtgaggagttgcttagttacataacgattttatgacgaaggcataacatgctgtagatcacatcaggtgaac                                         <  2:566970‑M1/240‑200 (MQ=255)                                                                                                                                                                                                    tgcccgccgGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccg                                     <  2:396425‑M1/246‑204 (MQ=255)                                                                                                                                                                                                     gCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGACTAACGCatcggcctcggattcacgtttatcagagttgtttttgggctggcagccagacgggagtcaggctgatattttgacaatactccgggttcgcgattctcgccataaccccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgacggcataacatgctgtagatcacatcaggtgaacgccgt                                    >  1:667762‑M1/1‑46 (MQ=255)                                                                                                                                                                                                     gCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcccatcaggtgaacgccgta                                   <  1:535168‑M1/251‑206 (MQ=255)                                                                                                                                                                                                      cTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgta                                   >  1:139320‑M1/1‑45 (MQ=255)                                                                                                                                                                                                         gCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgctgagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaa                               <  1:346635‑M1/251‑210 (MQ=255)                                                                                                                                                                                                          ccGGTGTTATAACGTTTTGCCGCCTGATCCTTGAAGAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaaa                              <  2:4757‑M1/251‑211 (MQ=255)                                                                                                                                                                                                            ggTGTTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaaaat                            >  1:88908‑M1/1‑39 (MQ=255)                                                                                                                                                                                                             gtgtTCTAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaatactttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaaaat                            >  2:52283‑M1/1‑38 (MQ=255)                                                                                                                                                                                                             gtgtTATAACGTTTTGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaaaata                           <  1:657316‑M1/251‑214 (MQ=255)                                                                                                                                                                                                                     aCGTTTTGCCGCCTGATCCTTGACTGACGCatcggcctcggatccacgtttctccgtgctgtttttgggctggcagccagaagggcgccaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaaaatatcttgtg                    >  2:597044‑M1/1‑30 (MQ=255)                                                                                                                                                                                                                           tGCCGCCTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggcggatattttgacaatattccgggttcgcgagtctcgccataacaccaaagaataatttgtagaggttatgagttgcttaggtacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaaaatatcttgtgattcag              <  1:431742‑M1/250‑227 (MQ=255)                                                                                                                                                                                                                               gccTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaaaatatcttgtgattcagatcac         >  2:65206‑M1/1‑20 (MQ=255)                                                                                                                                                                                                                                ccTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaaaatatcttgtgattcagatcac         >  1:165498‑M1/1‑19 (MQ=255)                                                                                                                                                                                                                                ccTGATCCTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagggagtcaggctgatattttgacaataatccggggtcgcgattctcgccataacaccaaagaataatttttagaggggatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggggaacgccgtaagaaaatatcttgtgattcagatcac         >  1:442121‑M1/1‑19 (MQ=255)                                                                                                                                                                                                                                      ccTTGAATAACGCatcggcctcggattcacgtttatcagtgttgtttttgggctggcagccagaagtgagtcaggctgatattttgacaataatccgggttcgcgattctcgccataacaccaaagaataatttttagaggtgatgagttgcttagttacataacgattgtatgacgaaggcataacatgctgtagatcacatcaggtgaacgccgtaagaaaatatcttgtgattcagatcacaaagatt  <  1:304176‑M1/251‑239 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      AAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACAGACAAACTGCTCTTTACGGAAGGTATAACCGCGCATTTCATAGCTTCCCTGGAACTGTTCGAAGGCGGTGACGTCGATTTTTTGCTCGCCGGTGTTATAACGTTTTGCCGCCTGATCCTTGCATAACGCTTCCATATTCAACGAACGCAGAGGATCAACCTTTACCCGTTGTGCTTTCTGCACGGGTGACGGTATAGAACAACCCACTAGCACCATAAGCGCCATTGCCGGAAAGAAAGCGTTCATCATCATTAAATTACCGTCTGGTTTGCAGGTGAGTCTTATTATTTATATCGGTATAGTGGCTGATATCTTACGAATTTCCTTAAGCCTCTATAAGCTGTAGTGATAAAACTCAGATTATTCC  >  NC_000913/3725348‑3725827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑