Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,027,2330TA34.5% 62.2 / 20.9 55intergenic (+5/+376)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base T (23/13);  new base A (0/19);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.42e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCA‑GGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGAT  >  NC_000913/4026984‑4027480
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           
aGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTTTTTCG‑GGGTCACCGGAAGCTGGCAGAGCCTGGTATCTATATTTTCGG‑GCG‑GTTTT‑TGATGG‑GGAAAA‑ATTCCC‑CCGATCTGTTTTGTC‑GAGAGCGGAATGTGCGGGGAGATCGCCC‑GTTTTG‑CGATGCG‑TTTTC‑ATTTATTTTAGatatt                                                                                                                                                                                                                                                            >  1:273704/1‑250 (MQ=255)
   cgcTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCA‑GGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATaaaa                                                                                                                                                                                                                                                         <  1:159197/251‑1 (MQ=255)
            tGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCA‑GGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTAGCTGAGATATAAAAAAACCCGc                                                                                                                                                                                                                                                 <  2:665131/250‑1 (MQ=255)
            tGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTACTGCAAGGA‑AACCGTGCGCCGGCGGAGCATGATATATTTATTTCCGG‑AGG‑TTTTT‑AGATGG‑GGAAAA‑TTGTCC‑CCCAACTGTTTTTGA‑GCGAGAGTAAAGAGCGGGGAGATCGCCTTGTTTGG‑‑GATGCG‑TTTGC‑ATTTATCTGTGAAATAAAAAAAACCGcc                                                                                                                                                                                                                                                >  1:322458/1‑251 (MQ=255)
                      tGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTTCC‑GGATCACGGTACGCTGGCAGAGCAAGATATCTTTATTGCCGG‑GCG‑TTTTT‑TGATGG‑CGAAAA‑TTGTCC‑GCGATCTGTTTTTCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTTG‑CGGTGCG‑TTTGC‑ATTTTTCTGTGGTATAAAAAAAACCCCCCCtcccacgg                                                                                                                                                                                                                                      >  1:529753/1‑244 (MQ=255)
                            gCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCA‑GGAGCACGGGACGCTGGGAGAGCAAGATATCTATATTTCCGG‑ACGGTTTTG‑‑GGTGG‑GGAAAAATTGCCC‑‑CGGGCTGTTTTGCT‑GTGAGCGGAAAGGGCGGGGAGATCGCCT‑GTTTGG‑CGAAGCGGTTTTC‑‑TTTATCTTGGATATAAAAAAAACccccccccacacgcgggg                                                                                                                                                                                                                                  >  1:301680/1‑237 (MQ=255)
                               agtgggggcGGTGGTTGAACAACCGGAGGCGGGCTGGCGTGGGCGTACTGGCACCGTTTTAACGGCGTTATTGCA‑GGATCACGGTCCGCTGGCAGAGCATAATATCTATATTGCCGG‑A‑G‑TTTTGGAGATGG‑CAAAAA‑TTCCCC‑GCGTTTTTTTTTGCA‑GGGGGCGTAATGCGCGGAAAAACCGCCT‑GTTGGG‑CGATGCG‑TTTGC‑TTTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAAc                                                                                                                                                                                                                            <  1:129749/243‑1 (MQ=255)
                                  ggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGAATTTCA‑GGGTCACGGTACGCTGGCAGAGGATGATATCTATATTTCCCG‑ACG‑TTTTT‑AGATGGAGGAAAA‑TT‑CCC‑GCGCTCTGTTTTGCC‑GGGAGCGTAATTGGCGGGGAGAGCGCCT‑GTTTGG‑CGATGCG‑TGTGC‑ATTTATTTGAGATATAAAAAAACACACCCCTGACAGGCGGg‑gagaaaagg                                                                                                                                                                                                                         >  1:569784/1‑247 (MQ=255)
                                      ccGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTTCA‑GGGTCACGGTCCGCCGGCAGAGCATGATATCTATATTTCCGG‑GCG‑GTTTG‑AGATGG‑GGAAAA‑TTTCCC‑CCGCTCTGTTTTTCC‑GTGAGCGGAAATCGCGGGGAGATCGCCC‑GTTTGG‑CGGTTCG‑TTTGC‑ATTTTTCTTGGGTATAAAAAAACCCCCCCCTGACAGGCGGGGAGGAACGGCacc                                                                                                                                                                                                                      >  1:312922/1‑249 (MQ=255)
                                               gAACACCCGGAGGCGGGTGGGGGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCA‑GGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGG‑ACT‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCTT‑GTTTGG‑CGATGCT‑TTTGC‑ATTTATCGGAGATATAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGtt                                                                                                                                                                                                            <  1:19131/250‑1 (MQ=255)
                                                           gggggcgggcGGGGGCGTACTGGCACCGTGTTAACGGCGGTATGGAA‑GGATCACGGTACGCTGGCAGAGAATGATATCTATATTGCCGG‑ACT‑TTTTG‑AGAGGG‑CAAAAA‑TTGCCC‑GCGTTCTGTTTTGCA‑GTGAGCGTAATGCGCGGAAAGATCGCCT‑GTTTGG‑CGAGGCG‑TTTGA‑ATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCAtt                                                                                                                                                                                               <  1:64214/249‑1 (MQ=255)
                                                                       ggggttacgggcagcgtttgaaCGGGGGTATTGGA‑GGAACACGGTAGGCTGGGAGAGCATGATATCTAAAGTCCCGG‑TCG‑TTTTG‑AGATGG‑CAAAAA‑TTGCCC‑GCGATTTGTTTGGCG‑GGGAGCGTAATGCGGGGAAAGATCGCCT‑TTTTGG‑CGATGCG‑TTTGA‑ATTTATCGGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGAc                                                                                                                                                                                   <  1:124793/231‑1 (MQ=255)
                                                                                gggcccgttttaagggggggtttGCG‑GGAACACGGTACGGGGGCAGAGCATAATATCTAGATTGCCGG‑CCG‑TTTGG‑AGAGGG‑CGAAAA‑TTGCCC‑GCGTTTTGTTTTGCA‑GTGGGCTTAAGGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTg                                                                                                                                                                            <  1:480928/228‑1 (MQ=255)
                                                                                  cgccGTGTTAAGGGGGGTATTGCA‑GGATCACGGTCCGCTGGCAGAGAATGATATTTATATGGCGGG‑ATG‑TTTTG‑AGATGG‑CAAAAA‑TTGCCC‑GGGATCTGTTTGGCG‑GTGGGCGTAAGGCGGGGGAAGACCGCTT‑GTTGGG‑CGATGCG‑TTGGC‑ATTTAGTGGATATAAAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCaa                                                                                                                                                                        <  1:666273/249‑1 (MQ=255)
                                                                                         ataccggcggTATTGCA‑GGATAACTGTCCGCTGGCAGGTCTTGATAGCTATTTTGCCGG‑AGT‑TTTTA‑AGATGG‑CGAAAT‑TTGCCG‑CCGATCTTTTTTGCA‑GGGAGCGTAGTGCGCGGGAAGATCGCTT‑TTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAAATATAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGc                                                                                                                                                                <  2:146864/247‑1 (MQ=255)
                                                                                              ggcggttgtGCA‑GGATCACGGTCCGCGGGAAGAGAATGATATCTAT‑TTGCCGGGACG‑TTGTG‑AGATGG‑GGAAAA‑TGGCCC‑GCAGTTTGTTT‑GCAAGTGAGCGTAATGCGGGGAAAGAGCGCGT‑GTTTGG‑CGATGGG‑TTTGC‑ATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGAt                                                                                                                                                           <  1:93597/243‑1 (MQ=255)
                                                                                               gcggTATGGCA‑GGATCCGGGTCCGCTGGGAGAGCATAATATCTATATGGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATTTGTTTGGCA‑GGGAGCGAAAGGCGCGGAAAGATCGCCT‑GTTTGG‑GGATGCG‑TTTGC‑ATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATg                                                                                                                                                          <  1:3498/251‑1 (MQ=255)
                                                                                                 ggTATTGCA‑GGCTCACGGTCCGCTGGCAGAGCATGATATCTATATTGACGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTTGAAAACCC‑TGACCGATGCg                                                                                                                                                        >  2:289302/1‑251 (MQ=255)
                                                                                                        cA‑GGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAAAGGCAACTAAAC‑TGTTATTCAGGGGCATTTAGATCTATGGAGTATCTGGCAAACCA‑TGCCCGGTGCGACGCTGt                                                                                                                                                 >  2:657062/1‑251 (MQ=255)
                                                                                                          gggATCACGGTGCGCTGGAAGAGCATGATATCTATATTGCCGG‑ACG‑TTTGG‑AGAGGG‑CAAAAA‑TTGCCC‑GCGATCTTTTTTGCA‑GTGAGCTTAATGCGGGGGAAGATCGCCT‑GTTTGG‑CGATGGG‑TTGGC‑TTTTATCGGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTc                                                                                                                                                <  1:72960/250‑1 (MQ=255)
                                                                                                           ggATCACGGTACGCTGGCCGAGCATGATATCTATATTGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑CAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTcg                                                                                                                                               >  1:139173/1‑251 (MQ=255)
                                                                                                                      cGCTGGCAGAGCATGATATCTATATTGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAA‑TGTTTTTCATTGGCATTTAGCTCCATGACGTATCCGGCAAACCA‑TTCCCGATGCGGCGCTGTCGCGTCCTAttgg                                                                                                                                    >  2:228024/1‑248 (MQ=255)
                                                                                                                         gggaggagCATGATCTCTAT‑TTGCCGGGACG‑TTGGG‑AG‑TGG‑GGAAAA‑ATGCCCCGCTATGTGTTTGGGA‑GGGGGCGTAGTGCGGGGGAGGATCGCCT‑TTTTGG‑GGATGTT‑TTTCC‑ATTTATCTGAGAAAAAAAAAAACCCGCCCCTAACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTAGTCAGGGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTTTCGCGTCTTATCGTGcc                                                                                                                                 <  2:348771/246‑1 (MQ=255)
                                                                                                                            cAGAGCATGATATCTATATTGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGAt                                                                                                                                                           >  1:343061/1‑222 (MQ=255)
                                                                                                                                 catgattgcTATTTTGCCGG‑GCG‑TTTTG‑AGATGG‑GGAAAA‑TTGCCC‑GCGATGTGTTTGGAA‑GGGGGGGTAATGCGGGGAAAAAGCGCTG‑GTTTGG‑CTAGGAG‑TTTGC‑TTTTATCGGAAAAATAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATa                                                                                                                         <  2:492083/243‑1 (MQ=255)
                                                                                                                                     atttttattttGCCGG‑ACT‑TTGTG‑AGATGG‑CAAAAA‑TTGCCC‑GCGATCTGTTTGGCA‑GTGAGGGTAATGCGGGGGAAGATCGCTT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATAAAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTcc                                                                                                                     <  1:558004/242‑1 (MQ=255)
                                                                                                                                     atatTTATATGGCCGG‑ACG‑TGTGG‑GGATGG‑TAAAAA‑GTGCCC‑GGGACTTTTTTTGGA‑GTGGGCGTAATGGGAGGAAAAATCGCCT‑TTTGGG‑CGATGCG‑TTTGC‑ATTGTTCGGAGAAAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAAGTAAAC‑TGTTATTGAGTGGTATTTAGATCTATTACTGATCTGGCAAACCA‑TGCCCGATGCGACGCTGGCGCGTCTGAGCGTGCCTACAGATATTcc                                                                                                                     <  2:569784/251‑1 (MQ=255)
                                                                                                                                           atatTGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAAATAAAA‑CGTTATTCAGTGGCATTTTGATCTATGACGGATCTGGCAAACCA‑TGCCCGATGCGACGCTTTTGCGTCTTTTCGTGCCTACCAACAATCCGaaacc                                                                                                               >  2:676046/1‑248 (MQ=255)
                                                                                                                                                    g‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCC‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTCTCTGAGATATACAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAT‑TGTTATTCAGTGGCATTTAGCTCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGGCTTCTCGTGTCTACAACTAGTCCGAACCGTAGGCCgg                                                                                                       >  1:639413/1‑250 (MQ=255)
                                                                                                                                                              g‑agaTGG‑CGAAAA‑TTGCCC‑GCGTTCTGTTTTGCA‑GTGAGCGAAATGCGCGGGAAGATCGC‑T‑GTTTGGGCGATGCG‑TTTGC‑ATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGt                                                                                             <  1:479390/251‑1 (MQ=255)
                                                                                                                                                                    gg‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCCAATAAAA‑TGTTATTCAGTGGCATTTAGATTTATGACGTATCTGGc                                                                                                                                                                          >  2:14250/1‑171 (MQ=255)
                                                                                                                                                                                gCCC‑GAGATCTGTTTTGCA‑GTGCGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGCACGGAAACTAAAC‑TGTTATTCCGTGGCATTTAAGTTTATGACGTATCTGGCAAACCA‑TGCCCGACGCGACGCTGTCGCGACATATCGCGCCTAACAATAGTCCGAACCGACGGCCGG‑ATTCGGCGGTTTCGCCGCATCCgg                                                                              >  2:608927/1‑251 (MQ=255)
                                                                                                                                                                                cccc‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTGGG‑CGATGCG‑TTTGC‑TTTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                              <  1:128766/250‑1 (MQ=255)
                                                                                                                                                                                 ccc‑GCGTTATGTTTGGCA‑GAGAGCGGAATGCGGGGGAAGATCCCCT‑GTTTGG‑‑GTTGGGGTTTGC‑ATTTTTCTGAAAAATAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGc                                                                             <  1:289396/251‑1 (MQ=25)
                                                                                                                                                                                     gcgttCTGTTTTGCG‑GTGAGCGTAATGCGCGGAAAGATCGCTT‑GTTGGT‑CAATGCG‑TTTGC‑ATTTATCGGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAAt                                                                          <  1:363781/247‑1 (MQ=37)
                                                                                                                                                                                       gATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGGAGGCCGG‑ATAAGGCGGTTACGCCGCATCCGGCAATTg                                                                        >  1:428787/1‑251 (MQ=255)
                                                                                                                                                                                       cgacttgttttgaaggaggggTAATGCGCGGGGAAAGCGCCT‑GTTTGG‑GGATGGG‑TGTCC‑ATTTATGGGAGAAAAAAAAAAAGCCGCCCCTGGCAGGCGGG‑AAGAACCGCAACTAAAC‑TGTTAATCAATGGCATTTAGATCTATGAAGGATCTGGCAAACCA‑TGCCCGGAGGGACGCTGGCGCGTGTTATCGTGCCTTCAAATAGTCCGGACCGTAAGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAAtt                                                                         <  1:371480/231‑1 (MQ=16)
                                                                                                                                                                                        aTCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAACAAACCAGCCCCTGACAGGCGGG‑AAGAACGGCAACTCAAC‑TGTTATTCCGCGGCCTTTTGCTCTATGACGTATCCGGCAAACCA‑TGCCCGATGCGAACCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑GTAAGGCGTTTAAGCCGCATCCGGCCATTgg                                                                       >  2:582230/1‑251 (MQ=37)
                                                                                                                                                                                              tttGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTAc                                                                                         >  1:522129/1‑227 (MQ=255)
                                                                                                                                                                                               ttGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCCACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAAACA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAAACGTAGGCCGG‑ATAAGGGGGTTACGCCGCATCCGGCAATTTGTGCatg                                                                 >  2:195263/1‑250 (MQ=255)
                                                                                                                                                                                                tGCA‑GTGAGCGTACTGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAACACAACCGCCCCTGCCAGGCGGG‑AAGAACGGGCACTAAACATGT‑CTTCAGTGGCATTTAGCTCTATGACCTTTATGGCCA‑CCACTGCCCGCTGCCACGCCGTCGCCTCTTATAGTTCCCACAACTAACCCGAACCGTAGGCCCG‑AATAGGCGTTTACGCCGCCTCCGGGCATTGGGGCatgtg                                                               >  2:622058/1‑249 (MQ=255)
                                                                                                                                                                                                 ggcagggggcggaaggcgcGGGAAGATCGCCT‑TTGGGG‑CGAGGCG‑TTTGC‑TTTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCatgat                                                               <  2:131893/236‑1 (MQ=25)
                                                                                                                                                                                                 ggagggggggtaaatggggggaaaGACCGCGT‑GTTGGG‑GGATGGG‑TTGGC‑ATTTATCTGAAAAAAAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCatga                                                                <  2:664695/228‑1 (MQ=21)
                                                                                                                                                                                                  gaagggatcGTAAGGCGGGGAAAGATCCCCT‑GTTTGG‑GGTTGCG‑TTTGC‑ATTTATCGAAGAAAAAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCatgat                                                               <  2:273704/242‑1 (MQ=25)
                                                                                                                                                                                                  caggTGAGCGTAGTGCGGGAAAAGATCGCCT‑GTTTGG‑CGATGGG‑TTGTC‑TTTTATCGGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCTTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCatgat                                                               <  1:289302/247‑1 (MQ=25)
                                                                                                                                                                                                       gAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGCACGGCAACTAAAA‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCCGGCAAACCA‑TGCCCGCTGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGtt                                                                                            >  2:477351/1‑216 (MQ=255)
                                                                                                                                                                                                        ggctaaaggcgggtgAAGATCGCTT‑TTTTGG‑CGGTGGG‑TGTGC‑ATTTATCTGAGATATAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGAt                                                        <  2:139173/237‑1 (MQ=25)
                                                                                                                                                                                                            tcattcgcgGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGCTGCg                                                     >  1:461583/6‑250 (MQ=255)
                                                                                                                                                                                                             aatggggggaaaGAGCGCGT‑GTTTGG‑CGATGCG‑TTTGC‑TTTTATGTGAGATAAAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAGCCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCGGCATCCTGCAATTGGTGCAGGATGCCTGATGCGa                                                    <  2:282629/240‑1 (MQ=25)
                                                                                                                                                                                                                 ggcgGGAATATCGCCT‑TTTTGC‑CGAT‑CG‑TTTGCCATTTATATGAAAAAAAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTg                                               <  1:323592/250‑1 (MQ=21)
                                                                                                                                                                                                                    gggAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTACCTGAGATATAAAAAAACCCGCCCCTGACAGGAGGG‑GAGAACGGCCACTCAAA‑TGTTATTTAGAGGCATTTAGATCTATGACGTAACTGGCAAACCC‑TGCCCGATGGGACGCTGTCGCGTCTTATAGTGCCTACAAAAAATCCCAACCCTAGGCCGGCGTACGGCGTTT‑CGCGGCATCCGGCAATTGGGGCATGATACCTGATgacacccggtcc                                            >  2:327739/1‑240 (MQ=255)
                                                                                                                                                                                                                             gCCT‑GTTGGG‑CGGTGCG‑TTGGA‑ATTTATGTGAGAAATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATc                                   <  1:389552/251‑1 (MQ=255)
                                                                                                                                                                                                                                  gTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAAACA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGCGCCTACAAATAGTCCGAAACGTAGGCCGG‑ACAAGGCGTTTACGCCGCATCCGGCAACTGGGGCCTGATGCCCTATGCGAGCCTGTGGCGTCTTATAGTGc                               >  2:380645/1‑251 (MQ=255)
                                                                                                                                                                                                                                      gg‑CGATGCG‑TTTGC‑ATTTATCTGAAAAAAAAAAAACCCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTa                            <  1:195263/250‑1 (MQ=255)
                                                                                                                                                                                                                                              gtttgtGC‑ATTTAGCTGGTATATAGAAAGGCCCGCGCGTGACAGGCGGG‑AAGAACGTCACCTAAAC‑TGTTATTCAGTGGAATTTAGATCTATGGCGTATCTGGCAAACCA‑TGCCCGATGCTACGCTGGCGCGTCTTATCGGGCCGACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGAGGCCTGATGCGACGCTGTCGCGTCTTATCGTTCCTACAAATAg                     <  2:454583/246‑1 (MQ=18)
                                                                                                                                                                                                                                                       aTTTATCTGATATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAAcc             <  1:235141/251‑1 (MQ=255)
                                                                                                                                                                                                                                                                  atatAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCCACTAAAA‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCCTGCCTACAAATAGTCCGAACCGTAGGCCGG‑CTAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGAt  >  1:425933/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                   tataAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCCCCTACAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTCGCAAACCA‑TGCCCGATGCGACCCTGTCGCGTCTTATCGTGCCTACAACTAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGACTCCGGCAATTGGGGCATGATGCCTGATGCGCCCCTGTCGCGGCTTATCGTGCCTACAAATAGTCCGAACCGCAGGCCgcat  >  1:457637/1‑247 (MQ=25)
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           
AGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCA‑GGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGG‑ACG‑TTTTG‑AGATGG‑CGAAAA‑TTGCCC‑GCGATCTGTTTTGCA‑GTGAGCGTAATGCGCGGGAAGATCGCCT‑GTTTGG‑CGATGCG‑TTTGC‑ATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGG‑AAGAACGGCAACTAAAC‑TGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCA‑TGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGG‑ATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGAT  >  NC_000913/4026984‑4027480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑